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Acta Pharmaceutica Sinica B 2021年第2期11卷 505-519页

作者： Kaiyue Yu Xiuming Yu Sisi Cao Yixuan Wang Yuanhao Zhai Fengdie Yang Xiaoyuan Yang Yi Lu Chuanbin wu yuehong Xu School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China

Psoriatic arthritis(PsA)is a complicated psoriasis comorbidity with manifestations of psoriatic skin and arthritic joints,and tailoring specific treatment strategies for simultaneously delivering different drugs to different action sites in PsA remains *** developed a need-based layered dissolving microneedle(MN)system loading immunosuppressant tacrolimus(TAC)and antiinflammatory diclofenac(DIC)in different layers of MNs,i.e.,TD-MN,which aims to specifically deliver TAC and DIC to skin and articular cavity,achieving simultaneous alleviation of psoriatic skin and arthritic joint lesions in *** vitro and in vivo skin permeation demonstrated that the inter-layer retained TAC within the skin of~100μm,while the tip-layer delivered DIC up to~300μm into the articular ***-MN not only efficiently decreased the psoriasis area and severity index scores and recovered the thickened epidermis of imiquimod-induced psoriasis but also alleviated carrageenan/kaolin-induced arthritis even better than DIC injection through reducing joint swelling,muscle atrophy,and cartilage ***,TD-MN significantly inhibited the serum TNF-αand IL-17 A in psoriatic and arthritic *** results support that this approach represents a promising alternative to multi-administration of different drugs for comorbidity,providing a convenient and effective strategy for meeting the requirements of PsA treatment.

关键词： Layered microneedles Need-based drug delivery Tacrolimus Diclofenac sodium Psoriasis Psoriatic arthritis

A new sulfur-doped source and synergistic effect with nitrogen for carbon dots produced from glucose

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Chinese Physics B 2019年第12期28卷 345-351页

作者： Lige Fu yuehong Yin Guohua Cao Pingping wu Jian Wang Lingling Yan Baoqing Zhang Ming Li School of Physics and Electronic Information Henan Polytechnic UniversityJiaozuo 454000China School of Materials Science and Engineering Henan Polytechnic UniversityJiaozuo 454000China

The nitrogen and sulfur co-doped carbon dots(N, S-CDs) with increased luminescence were synthesized by a hydrothermal process in one green pot by using glucose, and a new sulfur-doping source of sodium sulfite was *** synergistic effect of the N and S groups was well discussed through the structure analysis of Fourier transform infrared spectra and x-ray photoelectron spectra. The surface states of N, S-CDs embody more complicated functional groups, and S element exists as –SSO3, –C–SO3, and SO-42groups due to the introduction of sodium sulfite. The sulfur-containing groups passivate the surface of the CDs, and the relatively high sulfur groups may reduce the non-radiation centers. The fluorescence is affected by the hydroxyl group of the solvent. The quenching of Fe3+ ion to fluorescence and the sensitivity of fluorescence to p H were also investigated.

关键词： carbon dots glucose sodium sulfite nitrogen and sulfur codoping synergistic effect

基于网络药理学探讨柴芩温胆汤治疗糖尿病胃轻瘫的作用机制

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中国药物滥用防治杂志 2024年第1期30卷 48-51页

作者：蒋丽娜罗倩刘琴刘罗苗吴源陶周月红湖南中医药大学第一中医临床学院湖南长沙410007 湖南中医药大学第一附属医院湖南长沙410007 浏阳市中医医院湖南浏阳410300

目的:运用网络药理学方法探析柴芩温胆汤(CQWWD)治疗糖尿病胃轻瘫(DGP)的潜在作用机制。方法:利用中药系统药理学数据库和分析平台和本草组鉴获取CQWWD的主要活性成分和作用靶点,在GeneCards、OMIM、DisGeNET、Drug Bank、PharmGKB、TT... 详细信息

目的:运用网络药理学方法探析柴芩温胆汤(CQWWD)治疗糖尿病胃轻瘫(DGP)的潜在作用机制。方法:利用中药系统药理学数据库和分析平台和本草组鉴获取CQWWD的主要活性成分和作用靶点,在GeneCards、OMIM、DisGeNET、Drug Bank、PharmGKB、TTD数据库中预测DGP靶点,将药物作用靶点与DGP预测靶点取交集靶点,运用STRING数据库和Cytoscape3.7.2软件构建PPI网络,运用DAVID数据库行GO功能富集和KEGG通路富集分析。结果:筛选出CQWWD中的263个作用靶点,DGP疾病靶点共1439个,交集靶点61个,GO富集分析得到224个条目,涉及224个生物过程、18个细胞成分、38个分子功能,KEGG通路富集包含111条目。结论:本研究初步揭示CQWWD治疗DGP是多成分、多靶点、多通路的过程,为后续研究提供了支持。

关键词：柴芩温胆汤糖尿病胃轻瘫网络药理学作用机制

包头市某牧区小学生隐孢子虫和贾第鞭毛虫感染调查

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环境卫生学杂志 2020年第1期10卷 21-24页

作者：王华然杨忠委孙栋良杨栋金敏师丹阳武月红李君文尹静军事科学院军事医学研究院环境医学与作业医学研究所天津市300050 内蒙古科技大学包头师范学院

目的了解内蒙古牧区小学生隐孢子虫和贾第鞭毛虫感染情况,为预防工作提供科学依据。方法选取包头某旗小学牧区学生为对象,利用采样袋采集新鲜粪便,低温保存运至实验室。采用改良抗酸染色检测隐孢子虫,卢戈氏碘液染色检测贾第鞭毛虫。结... 详细信息

目的了解内蒙古牧区小学生隐孢子虫和贾第鞭毛虫感染情况,为预防工作提供科学依据。方法选取包头某旗小学牧区学生为对象,利用采样袋采集新鲜粪便,低温保存运至实验室。采用改良抗酸染色检测隐孢子虫,卢戈氏碘液染色检测贾第鞭毛虫。结果共采集小学生粪便样本191份,隐孢子虫总感染率为37.2%(71/191),男生和女生的感染率分别为40.0%(44/110)和33.3%(27/81),差异无统计学意义(χ^2=0.888,P>0.05);贾第鞭毛虫总感染率为14.7%(28/191),男生和女生的感染率分别为14.5%(16/110)和14.8%(12/81),差异无统计学意义(χ^2=0.003,P>0.05);隐孢子虫和贾第鞭毛虫混合感染率为5.8%(11/191),男生和女生的混合感染率分别为7.3%(8/110)和3.7%(3/81),差异无统计学意义(χ^2=1.095,P>0.05)。结论牧区生源小学生中隐孢子虫和贾第鞭毛虫感染率较高,当地卫生部门应加强卫生宣传和管理。

关键词：隐孢子虫贾第鞭毛虫小学生牧区感染率

Comparative pharmacokinetics of tetramethylpyrazine phosphate in rat plasma and extracellular fluid of brain after intranasal,intragastric and intravenous administration

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Acta Pharmaceutica Sinica B 2014年第1期4卷 74-78页

作者： Dongmei Meng Haoyang Lu Shanshan Huang Minyan Wei Pingtian Ding Xianglin Xiao yuehong Xu Chuanbin wu School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China The First Affiliated Hospital of Guangzhou Medical College Guangzhou 510120China School of Pharmacy Shenyang Pharmaceutical UniversityShenyang110016China

The purpose of this study was to compare the pharmacokinetic profiles of tetramethylpyrazine phosphate(TMPP)in plasma and extracellular fluid of the cerebral cortex of rats via three delivery routes:intranasal(i.n.),intragastric(i.g.)and intravenous(i.v.)*** i.n.,*** *** of a single-dose at 10 mg/kg,cerebral cortex dialysates and plasma samples drawn from the carotid artery were collected at timed *** concentration of TMPP in the samples was analyzed by *** area under the concentration-time curve(AUC)and the ratio of the AUCbrain to the AUCplasma(drug targeting efficiency,DTE)was calculated to evaluate the brain targeting efficiency of the drug via these different routes of *** ***,TMPP was rapidly absorbed to reach its peak plasma concentration within 5 min and showed a delayed uptake into cerebral cortex(t_(max)=15 min).The ratio of the AUCbrain dialysates value between *** and *** was 0.68,which was greater than that obtained after ***(0.43).The systemic bioavailability obtained with *** was greater than that obtained by the ***(86.33%vs.50.39%),whereas the DTE of the nasal route was 78.89%,close to that of oral administration(85.69%).These results indicate that TMPP is rapidly absorbed from the nasal mucosa into the systemic circulation,and then crosses the blood-brain barrier(BBB)to reach the cerebral *** administration of TMPP could be a promising alternative to intravenous and oral approaches.

关键词： Tetramethylpyrazine phosphate Intranasal delivery Pharmacokinetics Microdialysis Rat

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山羊卵母细胞的体外成熟研究

中国农学通报 2005年第9期21卷 1-3,16页

作者：赵晓娥李向臣高立功吴月红山灵刘凤军王永胜张涌西北农科技大学生物工程研究所陕西杨凌712100

1.比较了在卵母细胞成熟液中添加相同浓度(10ng/ml)的表皮生长因子(EGF),胰岛素样生长因子(IGF),EGF+IGF后,不同生长因子对卵母细胞体外发育率的影响。结果表明,在成熟培养液中添加EGF后,其卵母细胞的成熟率(70.9%)与对照组(67.3%)相比... 详细信息

1.比较了在卵母细胞成熟液中添加相同浓度(10ng/ml)的表皮生长因子(EGF),胰岛素样生长因子(IGF),EGF+IGF后,不同生长因子对卵母细胞体外发育率的影响。结果表明,在成熟培养液中添加EGF后,其卵母细胞的成熟率(70.9%)与对照组(67.3%)相比有所提高,但差异不显著;在成熟培养液中分别添加IGF、EGF+IGF,其卵母细胞的成熟率分别为80.0%,82.4%,显著高于对照组卵母细胞的成熟率(67.3%)。2.比较了不同季节采集的卵母细胞在体外成熟的情况。结果发现,12月份到来年的1月份,采集的卵母细胞,其体外成熟率最高,为81.0%;11月份采集的卵母细胞,其体外成熟率次之,为74.1%;3—5月份和9—10月份采集的卵母细胞,其体外成熟率为66.0%、69.6%;6—7月初采集的卵母细胞,其体外成熟率最差,为54.7%。3.比较了OM液pH值对卵母细胞体外成熟的影响。当OM培养液的pH值为7.2和6.8￣7.0时,卵母细胞成熟率的为66.2%和61.0%,显著高于pH≥7.5时的成熟率(50.9%,P<0.05)。

关键词：羊卵母细胞体外成熟 EGF IGF 季节 pH值卵母细胞成熟胰岛素样生长因子表皮生长因子

山羊转t-PA突变体基因的体细胞核移植研究

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中国农学通报 2005年第6期21卷 1-4页

作者：赵晓娥安志兴李向臣高立功吴月红山灵刘凤军王永胜张涌西北农科技大学生物工程研究所陕西杨凌712100

比较了用转t-PA突变体基因的胎儿成纤维细胞和卵丘细胞作供体时核移胚的发育情况。结果表明:卵丘细胞作供体时,核移胚的卵裂率(82.9%)和桑葚胚率(55.9%)均高于胎儿成纤维细胞卵裂率(75.7%)和桑葚胚率(48.7%),但差异不显著(P>0.05);卵丘... 详细信息

比较了用转t-PA突变体基因的胎儿成纤维细胞和卵丘细胞作供体时核移胚的发育情况。结果表明:卵丘细胞作供体时,核移胚的卵裂率(82.9%)和桑葚胚率(55.9%)均高于胎儿成纤维细胞卵裂率(75.7%)和桑葚胚率(48.7%),但差异不显著(P>0.05);卵丘细胞作供体时的囊胚率(19.7%)则高于胎儿成纤维细胞(11.0%),差异显著。(P0.05);用SOF液培养时,添加10%bFF,其囊胚率(11.2%)高于添加10%FBS(5.5%)(P>0.05)。说明用SOF液优于CR1aa液。

关键词：变体基因体细胞核移植胎儿成纤维细胞 PA 山羊卵丘细胞供体细胞囊胚率卵裂率差异显著饥饿处理转基因 SOF 添加桑葚发育

血清降钙素原和高敏C反应蛋白在革兰氏阴性菌感染的腹膜透析相关性腹膜炎中的临床应用

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中国医药导报 2024年第22期21卷 108-113页

作者：王月洪由天辉吴志敏锁丹黄柳齐高丽梅戴美芬郑晶广东药科大学护理学院广东广州510310 广东药科大学继续教育学院广东广州510240 广东省佛山市南海区人民医院护理部广东佛山528200 广东省佛山市南海区人民医院肾内科广东佛山528200

目的探讨血清降钙素原(PCT)、高敏C反应蛋白(hs-CRP)在革兰氏阴性菌感染的腹膜透析相关性腹膜炎(PDAP)中的临床应用价值。方法回顾性分析2017年12月至2023年8月广东省佛山市南海区人民医院收治的165例腹膜透析患者的临床资料,根据培养... 详细信息

目的探讨血清降钙素原(PCT)、高敏C反应蛋白(hs-CRP)在革兰氏阴性菌感染的腹膜透析相关性腹膜炎(PDAP)中的临床应用价值。方法回顾性分析2017年12月至2023年8月广东省佛山市南海区人民医院收治的165例腹膜透析患者的临床资料,根据培养结果将其分为革兰氏阴性菌组和非革兰氏阴性菌组;比较两组一般资料及实验室检查指标;采用多因素logistic回归分析革兰氏阴性菌感染PDAP发生的影响因素;受试者操作特征(ROC)曲线评价PCT、hs-CRP对革兰氏阴性菌感染PDAP的预测价值。结果165例腹膜透析患者共发生268例次PDAP,其中革兰氏阳性菌127例次、革兰氏阴性菌69例次、真菌5例次、分枝杆菌1例次、混合感染11例次、未找到病原菌55例次。两组PCT、hs-CRP、中性粒细胞与淋巴细胞比值及估算肾小球滤过率比较,差异有统计学意义(P<0.05)。多重共线性分析显示,方差膨胀因子均<10,提示各自变量间不存在多重共线性。多因素logistic回归分析显示,PCT(OR=1.085,95%CI:1.038~1.134,P<0.001)、hs-CRP(OR=1.008,95%CI:1.003~1.013,P=0.003)是革兰氏阴性菌感染PDAP发生的独立影响因素。ROC曲线分析显示,PCT联合hs-CRP对革兰氏阴性菌感染PDAP的诊断预测价值高于各项单独预测。结论PCT、hs-CRP是革兰氏阴性菌感染PDAP发生的独立影响因素,PCT联合hs-CRP对革兰氏阴性菌致PDAP具有较高预测价值。

关键词：腹膜透析腹膜透析相关性腹膜炎革兰氏阴性菌降钙素原高敏C反应蛋白

An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

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Virologica Sinica 2022年第1期37卷 138-141页

作者： Ying Tang Yiqin Wang Yuchang Li Huai Zhao Sen Zhang Ying Zhang Jing Li yuehong Chen Xiaoyan wu Chengfeng Qin Tao Jiang Xiaoping Kang Department of Virology Beijing Institute of Microbiology and EpidemiologyAMMSBeijing 100071China State Key Laboratory of Pathogen and Biosecurity AMMSBeijing 100071China Beijing University of Chemical Technology Beijing 100029China Technology Center of Erenhot Customs Erenhot 011100China Lifereal Biotech.Inc. Hangzhou 311100China Anhui Medical University Hefei 230032China

Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-Co V-2)is a novel coronavirus that causes the outbreak of coronavirus disease 2019(COVID-19)(Li et al.,2020a).Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19(wu et al.,2020a;Zhu et al.,2020).Currently,a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection(Corman et al.,2020;Hussein et al.,2020;Ruhan et al.,2020;Veyer et al.,2020).Conventional qPCR involves virus inactivation,nucleic acid extraction,and qPCR amplification ***,the process is complicated,which usually takes longer than 2 h,and requires biosafety laboratories and professional ***,qPCR is not suitable for use in field or medical units.

关键词： al. diagnosis acute

GCK合并HNF-1B基因突变致青少年起病的成人型糖尿病家系1例

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皖南医学院学报 2024年第5期43卷 509-510,F0003页

作者：朱钥红周梦云吴涵翟清张安素姚新明高家林皖南医学院第一附属医院弋矶山医院内分泌科安徽芜湖241001

目的:分析葡萄糖激酶(GCK)合并肝细胞核因子1B(HNF-1B)基因突变致青少年起病的成人型糖尿病(MODY)家系1例。方法:对先证者及其父母、弟弟完善静脉血全外显子基因检测,先证者明确诊断为GCK-MODY合并HNF-1B-MODY。结果:患者及其母亲均发生... 详细信息

目的:分析葡萄糖激酶(GCK)合并肝细胞核因子1B(HNF-1B)基因突变致青少年起病的成人型糖尿病(MODY)家系1例。方法:对先证者及其父母、弟弟完善静脉血全外显子基因检测,先证者明确诊断为GCK-MODY合并HNF-1B-MODY。结果:患者及其母亲均发生GCK、HNF-1B基因突变,患者弟弟发生CGK基因突变。结论:基因检测对诊断MODY至关重要,需重视患者的早期诊断,减少误诊、误治。

关键词：青少年起病的成人型糖尿病葡萄糖激酶肝细胞核因子1B