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Molecular Plant 2012年第1期5卷 270-280页

作者： Zi-yu li Bin li Ai-wu Dong State Key Laboratory of Genetic Engineering Institute of Plant BiologyDepartment of BiochemistrySchool of Life SciencesFudan UniversityShanghai200433China

Plant cells frequently undergo endoreduplication, a modified cell cycle in which genome is repeatedly rep- licated without cytokinesis. As the key step to achieve final size and function for cells, endoreduplication is prevalent during plant development. However, mechanisms to control the balance between endoreduplication and mitotic cell di- vision are still poorly understood. Here, we show that the Arabidopsis TCP （CINCINNATA-like TEOSINTE BRANCHED1~ CYCLOIDEA-_PCF）-family transcription factor gene AtTCP15 is expressed in trichomes, as well as in rapidly dividing and vascular tissues. Expression of AtTCP15SRDX, AtTCP15 fused with a SRDX repressor domain, induces extra endoredupli- cation in trichomes and cotyledon cells in transgenic Arabidopsis. On the contrary, overexpression of AtTCP15 suppresses endoreduplication in trichomes and other examined cells. Misregulation of AtTCP15 affects the expression of several im- portant genes involved in cell-cycle regulation. AtTCP15 protein binds directly to the promoter regions of CYCA2.3 and RETINOBLASTOMA-RELATED （RBR） genes, which play key roles in endoreduplication. Taken together, AtTCP15 plays an important role in regulating endoreduplication during Arabidopsis development.

关键词： Arabidopsis TCP transcription factor endoreduplication cell cycle gene expression.

Fabrication of stable MWCNT bucky paper for solar-driven interfacial evaporation by coupling c-ray irradiation with borate crosslinking

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Nuclear Science and Techniques 2021年第12期32卷 13-25页

作者： yu-Qing Qiao yu Gu yu-Sen Meng Hai-Xia li Bo-wu Zhang Jing-Ye li MOE Key Lab of Resource Chemistry Shanghai Key Lab of Rare Earth Functional MaterialsCollege of Chemistry and Materials ScienceShanghai Normal UniversityShanghai 200234China Yellow River Institute of Eco-Environmental Research Yellow River Basin Ecology and Environment AdministrationZhengzhou 450000China shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China School of Civil and Architectural Engineering Nanjing Tech University Pujiang InstituteNanjing 211134China

Herein,we report a facile solution process for preparing multi-walled carbon nanotube(MWCNT)bucky paper for solar-driven interfacial water *** process involves vacuum filtrating a dispersion of MWCNTs that was modified by polyvinyl alcohol(PVA)under c-ray irradiation on a cellulose acetate microporous membrane,followed by borate *** transform infrared spectroscopy,Raman spectroscopy,and thermogravimetry confirmed the success of PVA grafting onto MWCNTs and borate crosslinking between modified MWCNT *** as-prepared crosslinked MWCNT bucky papers(BBP membranes)were used as a solar absorber,by placing them on a paper-wrapped floating platform,for interfacial water evaporation under simulated solar *** BBP membranes showed good water tolerance and mechanical stability,with an evaporation rate of 0.79 kg m^(-2.h^(-1)and an evaporation efficiency of 56%under 1 sun illumination in deionized ***,the BBP membranes achieved an evaporation rate of 0.76 kg m^(-2.h^(-1)in both NaCl solution(3.5 wt%)and sulfuric acid solution(1 mol L-1),demonstrating their impressive applicability for water reclamation from brine and acidic *** evaporation rate of 0.70 kg m-2.h-1(very close to that from deionized water)was obtained from the solar evaporation of saturated NaCl solution,and the BBP membrane exhibited unexpected stability without the inference of salt accumulation on the membrane surface during long-term continuous solar evaporation.

关键词： c-ray irradiation Multi-walled carbon nanotubes Bucky paper Solar-driven interfacial water evaporation Desalination

Treatment of Anthraquinone Dyeing Wastewater by Microelectrolysis and Coagulation Process

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Treatment of Anthraquinone Dyeing Wastewater by Microelectro...

亚太绿色氧化还原技术国际研讨会

作者： wu yun-tao1* Chu Jin-yu1 wu Chun-du1 li Ning1 Yang Jian2.1School of Environment Jiangsu University Zhenjiang Jiangsu 2.2.13 china 2.ollege of Environmental Science and Engineering Tongji University shanghai 2.0092.china

The dyeing wastewater with anthraquinone is a highly basic effluent that is bio- refractory (B/C = 0.079~0.136). The wastewater was treated by the microelectrolysis and flocculation process. Influence

关键词： dyeing wastewater anthraquinone microelectrolysis coagulation

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《疟原虫核酸检测多重PCR法》标准解读

中国寄生虫学与寄生虫病杂志 2024年第4期42卷 521-524,528页

作者：王真瑜江莉余晴张耀光吴寰宇陈健朱民上海市疾病预防控制中心上海200336

遵循《团体标准化第1部分:良好行为指南》(GB/T 2.004.1—2.16)相关规定,按照《标准化工作导则》(GB/T 1.1—2.09)体例编制《疟原虫核酸检测多重PCR法》(T/SPMA 004—2.2.)。标准由7章组成,包括“适用范围”“规范性引用文件”“术语和... 详细信息

遵循《团体标准化第1部分:良好行为指南》(GB/T 2.004.1—2.16)相关规定,按照《标准化工作导则》(GB/T 1.1—2.09)体例编制《疟原虫核酸检测多重PCR法》(T/SPMA 004—2.2.)。标准由7章组成,包括“适用范围”“规范性引用文件”“术语和定义”“仪器设备”“试剂材料”“检测步骤”和“废弃物的处理和防止污染的措施”,另附有1个资料性附录(技术原理)和4个规范性附录(2.检测方法、试剂配制、样本准备)。该标准已由上海市预防医学会发布公告(沪预医会[2.2.]34号)实施,为各级疾病预防控制机构和医疗机构规范操作、提高样本检测质量提供了技术依据,弥补了《疟疾的诊断》(WS2.9—2.15)核酸检测尚无细化操作步骤之不足。

关键词：疟原虫核酸 PCR 标准

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基于潮流模拟的琼州海峡东口动力地貌分析

海洋地质前沿 2024年第9期40卷 38-48页

作者：仝长亮吴祥柏陈飞朱钰李高聪贾建军海南省海洋地质资源与环境重点实验室海口570206 海南省地质测试研究中心海口570206 海南省海洋地质调查院海口570206 江苏科技大学船舶与海洋工程学院镇江519082 广东海洋大学电子与信息工程学院湛江524088 华东师范大学海洋科学学院上海200241

潮流是塑造琼州海峡东口海底地貌的主要动力,研究潮流作用下的沉积物运动特征,有助于更好地理解海峡的水沙通量、沉积过程和地貌演化。根据对2.08和2.15年研究区2.9个站位所采集的表层样品调查数据的研究统计,海峡东口沉积物以砂砾质为... 详细信息

潮流是塑造琼州海峡东口海底地貌的主要动力,研究潮流作用下的沉积物运动特征,有助于更好地理解海峡的水沙通量、沉积过程和地貌演化。根据对2.08和2.15年研究区2.9个站位所采集的表层样品调查数据的研究统计,海峡东口沉积物以砂砾质为主,其分布规律与潮流的强弱和方向关系密切,运动形式主要为推移方式。为了进一步研究沉积物的运移与海底地貌的动态关系,利用沉积动力学模型估算了琼州海峡东口的潮流过程、沉积物输运率和侵蚀淤积强度等指标。结果显示,大潮时,东向急流的流速为0.9~2.0 m/s,西向急流为0.8~1.7 m/s,中潮和小潮的潮流流速约为大潮时的50%~60%。总体上,东向流强于西向流,但在海峡北岸和中央水道,西向流始终强于东向流。大潮时,全区沉积物的活动频率普遍较高,均值可达60%,其平均输运率在10^(−2.kg/(m·s)量级;而中小潮期间,活动频率均值仅为18%左右,平均输运率比大潮期小1~2.数量级。沉积物整体输运方向为东向,但在海峡北岸净输运方向为西向。从侵蚀淤积强度的分布可知,研究区的浅滩堆积速率较高,水道的侵蚀速率较高,量级可达10^(−1) m/a。

关键词：动力地貌推移质模拟潮流模拟琼州海峡东口

上海某社区老年人全氟和多氟烷基化合物摄食暴露风险评估

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生态毒理学报 2024年第4期19卷 182-193页

作者：李彤王宇吴限好陈绩林楠冯楠楠上海交通大学新华临床医学院上海200092 上海交通大学仁济临床医学院上海200127 上海交通大学公共卫生学院上海200025

目前在人群体内可广泛检测出全氟和多氟烷基化合物(per-and poly-fluoroalkyl substances,PFAS),但对高年龄层人群PFAS的暴露特征研究有限。本文旨在评估上海老年人群PFAS摄食暴露风险及其来源特征。采用横断面调查研究方法,共获取上海... 详细信息

目前在人群体内可广泛检测出全氟和多氟烷基化合物(per-and poly-fluoroalkyl substances,PFAS),但对高年龄层人群PFAS的暴露特征研究有限。本文旨在评估上海老年人群PFAS摄食暴露风险及其来源特征。采用横断面调查研究方法,共获取上海市60~79岁老人有效问卷68份,并结合“第六次全国总膳食研究”中PFAS膳食浓度,应用蒙特卡洛模型对膳食暴露量进行概率计算,最终根据欧洲食品安全局发布的4种PFAS,包括全氟辛酸(perfluorooctanoic acid,PFOA)、全氟辛烷磺酸(perfluorooctane sulfonic acid,PFOS)、全氟己烷磺酸(perfluorohexane sulfonic acid,PFHxS)和全氟壬酸(perfluorononanoic acid,P FNA)每周可耐受摄入量评估健康风险。结果表明,该人群膳食结构以水果(1440.0 g·week^(-1))、谷物(1341.0 g·week^(-1))和奶制品(835.0 g·week^(-1))为主,分别占总膳食的2..8%、2..1%和14.4%;4种PFAS总摄食暴露量为(102..7±140.3)pg·kg^(-1)·d^(-1),水产品贡献最多(53.5%);PFOA、PFOS、PFHxS和PFNA的摄食暴露量分别为(308.4±36.5)、(519.2.104.2.、(49.8±7.6)和(73.3±13.6)pg·kg^(-1)·d^(-1),鸡蛋(48.9%)、水产品(75.1%)、谷物(64.9%)和水产品(76.3%)分别为PFOA、PFOS、PFHxS和PFNA的主要贡献源。PFOA和PFOS摄食暴露导致的健康风险(health risk,HR)分别为0.36和0.32.鸡蛋(48.9%)和水产品(75.1%)分别为最大贡献者。4种PFAS摄食暴露的总健康风险为1.63,水产品贡献最大(53.5%),其次是鸡蛋(2..9%)和肉类(15.5%)。男女人群中各PFAS导致的健康风险相对关系基本与总人群一致,女性人群由水产品贡献的暴露风险显著高于男性(均值67.3%>43.8%,P1)。综上,上海老年人群的PFAS摄食暴露风险普遍较高,水产品是4种PFAS包括PFOS的主要暴露来源,鸡蛋和肉类对于PFOA的暴露更加突出。本研究明晰了上海地区老年人群的PFAS摄食暴露风险和来源特征,为全氟化合物的来源、累积及迁移规律的研究提供基础数据。

关键词：全氟和多氟烷基化合物老年人群健康风险评估膳食暴露概率评估

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Rab5 dependent membrane endocytosis is required for Semaphorin3A induced axon growth cone collapse

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Rab5 dependent membrane endocytosis is required for Semaphor...

针刺治疗子宫内膜异位症相关性不孕:一项随机对照试验研究方案

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Kong-Yan wu~1,Ai-li SHENG~1,Wen-Wen liU~2.An-Qi GENG~1,Xing-yu JIANG~2.Xiao-Bing yuAN~1, Zhen-Ge LUO~(1,*) 1 Institute of Neuroscience,State Key Laboratory of Neuroscience,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 2.0031,china 2.CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety,National Center for NanoScience and Technology,Beijing 100190,china

＜正＞Guidance cues are critical for axon navigation and ultimately for the wiring of the neuro-circuits during nervous system *** cones of growing axons can sense both attractive and repulsive cues in the environment and make a corresponding ***3A,a negative guidance cues,can collapse growth cones of outgrowing axons,however,the intracellular molecular mechanisms are not well *** we show that the small GTPase Rab5 is involved in growth one collapse in response to ***5 activity assay shows that Rab5 is transiently activated after treatment with *** cone collapse is abolished by down-regulation of Rab5 by specific siRNA in response to *** advantage of live cell FRET assay,we demonstrate that semaphorin3A can activate Rab5 and promote the retrograde trafficking of Rab5 positive endosomes in growth ***5 is required for endocytosis of the growth cone plasma membrane during growth cone collapse. Over-expression of Rab5 leads to axon growth cones collapse in vitro and impedes projecting axons growth in vivo. These findings indicate that in response to negative guidance molecules semaphorin3A,axon growth cones driving endocytosis of the plasma membrane associated with the activated function of Rab5 resulted in growth cone collapse.

关键词： growth cone Semaphorin3A Rab5 endocytosis plasma membrane FRET microfluidic culture system electroporation

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World Journal of Acupuncture-Moxibustion 2024年第2期34卷 156-162页

作者：吴楚婷董晓燕李欣荣曹阳黄彩梅李铭旸胡佳玮高源李语然吴焕淦张春雁上海中医药大学附属岳阳中西医结合医院上海200437 复旦大学附属妇产科医院上海200011 上海市长宁区妇幼保健院上海200051

Background:Acupuncture has been proven to improve the pregnancy rate in infertile *** is a common cause of *** far,there are no studies of the efficacy of acupuncture on endometriosis-associated infertility(EAI).Therefore,we will conduct a randomized controlled trial(RCT)to investigate whether acupuncture could have higher pregnancy rates in patients with EAI compared with sham *** study protocol is reported in this ***/design:The current trial is a multicenter randomized controlled study undergoing in shanghai,*** 2.4 eligible patients with EAI will be randomly assigned into the acupuncture group and the sham acupuncture group in a 1:1 *** the acupuncture group,patients will be treated with acupuncture on Guanyuan(CV4),Zhongji(CV3),Zigong(EX-CA1),Xuehai(SP10),Zusanli(ST36),Sanyinjiao(SP6),and Taichong(LR3),twice a week,for 3 menstrual cycles,and 12.menstrual cycles will be followed *** Park sham device will be used to perform non-transdermal acupuncture in the sham acupuncture *** operation points will be located at 1.5 cm away beside these acupoints in the acupuncture *** intervention period is the same as that in the acupuncture *** primary outcome will be pregnancy *** outcomes will include ultrasound results,cancer antigen 12.(CA12.),antiMullerian hormone(AMH),sex hormones,and the Endometriosis Health Profile-5(EHP-5).Outcomes will be collected at baseline,the end of treatment,and follow-up visits at 3,6,and 12.menstrual *** data,including major adverse events,will be recorded in electronic case report forms and analyzed using SPSS ***:This study will help to evaluate whether acupuncture is effective in increasing the pregnancy rate of infertile women with endometriosis.

关键词： Endometriosis-associated infertility(EAl) Acupuncture Randomized controlled trials(RCTs) Protocol

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The FAST Core Array

astronomical Techniques and Instruments 2024年第2期1卷 84-94页

作者： Peng Jiang Rurong Chen Hengqian Gan Jinghai Sun Boqin Zhu Hui li Weiwei Zhu Jingwen wu Xuelei Chen Haiyan Zhang Tao An National astronomical Observatories Chinese Academy of SciencesBeijing 100101China Guizhou Radio astronomical observatory Guizhou UniversityGuiyang 550000China shanghai astronomical {2. Chinese Academy of SciencesShanghai 200030China

The Five-hundred-meter Aperture Spherical Radio Telescope(FAST)Core Array is a proposed extension of FAST,integrating 2. secondary 40-m antennas implanted within 5 km of the FAST *** original array design will combine the unprecedented sensitivity of FAST with a high angular resolution(4.3"at a frequency of 1.4 GHz),thereby exceeding the capabilities at similar frequencies of next-generation arrays such as the Square Kilometre Array Phase 1 or the next-generation Very Large *** article presents the technical specifications of the FAST Core Array,evaluates its potential relatively to existing radio telescope arrays,and describes its expected scientific *** proposed array will be equipped with technologically advanced backend devices,such as real-time signal processing systems.A phased array feed receiver will be mounted on FAST to improve the survey efficiency of the FAST Core Array,whose broad frequency coverage and large field of view(FOV)will be essential to study transient cosmic phenomena such as fast radio bursts and gravitational wave events,to conduct surveys and resolve structures in neutral hydrogen galaxies,to monitor or detect pulsars,and to investigate exoplanetary ***,the FAST Core Array can strengthen china's major role in the global radio astronomy community,owing to a wide range of potential scientific applications from cosmology to exoplanet science.

关键词： FAST Radio telescope Interferometry Synthesis array

First report of microcystin production in Microcystis smithii Komrek and Anagnostidis(Cyanobacteria) from a water bloom in Eastern china

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Journal of Environmental Sciences 2011年第1期23卷 102-107页

作者： Yang liu Wenhua Tan Xingqiang wu Zhongxing wu Gongliang yu Renhui li Key Laboratory of Aquatic Biodiversity and Conservation Biology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

A water bloom sample collected from Lake Dishui in shanghai was characterized. The morphological identification showed that Micorcystis wesenbergii and Micorcystis smithii were the main component of the bloom. Five strains of M. smithii were successfully isolated. Their 16S rRNA gene sequences based phylogenetic tree showed that the five strains of M. smithii intermixed with strains of other morphospecies in Microcystis. A fragment of mcy gene encoding for microcystin synthetase was detected in one of the five M. smithii strains （CHAB 2.83）, indicating its potential of microcystin production. High performance liquid chromatography analysis confirmed M. smithii CHAB 2.83 to produce microcystin-RR as 1550 μg per gram dry weight cells. The present investigation, for the first time, reported the isolated strains of M. smithii and microcystin production from M. smithii.

关键词： Cyanobacteria Microcystis smithii microcystin-RR mcyA 16S rRNA gene