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Determination of 23 β_2-agonists and 5 β-blockers in animal muscle by high performance liquid chromatography-linear ion trap mass spectrometry

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Science china Chemistry 2010年第4期53卷 832-840页

作者： wu yongning1,2, miao hong1, fan sai1,2 & zhao yunfeng1,2 1 institute of nutrition and food safety, chinese centre for disease control and prevention, beijing 100021, china 2 College of Veterinary Medicine, china Agricultural University, beijing 100193, china 1. institute of nutrition and food safety Chinese Centre for Disease Control and Prevention Beijing 100021 China2. College of Veterinary Medicine China Agricultural University Beijing 100193 China

A high performance liquid chromatography-linear ion trap mass spectrometry method using isotope dilution technique has been developed for the simultaneous determination of 23 β2-agonists and 5 β-blockers in animal muscle tissues. Pork and chicken muscle samples were acid hydrolyzed and extracted with 5% trichloracetic acid in water, and then cleaned up using MCX solid phase extraction (SPE) cartridge. Methanol and 0.1% formic acid were used as mobile phases for gradient elution. A Waters AtlantisT3 column was used for separation. ESI positive ion scan mode was used with selective reaction monitoring. 9 β2-Agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 23 β2-agonists and 5 β-blockers were 5-200 μg/L, the coefficient of correlation was not less than 0.995, and the limit of detection for each compound in the muscle tissue was below 0.2 μg/kg. The recoveries of each compound in the spiked samples at three levels 5, 10, 20 μg/kg were in the range of 47.3%-123.7%, and the relative standard deviations were in the range of 3.2%-25.7%. The developed method is sensitive and specific for the determination of β2-agonists and β-blockers in pork and chicken muscle samples.

关键词： β2-agonists β-blockers muscle determination HPLC-ITMS

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