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Cell Research 1997年第2期7卷 207-215页

作者： wu ya lan GUO HUANG FAN JIAN ZHAO YIZHANG TIAN HUA ZHOU lan MA GANG PEI(Shanghai Institute of Cell Biology, Chinese Academy ofSciences, Shanghai 200031, ChinaShanghai Medical University, Shanghai 200032, China) Shanghai Medical University Shanghai 200032 China

Expression of opioid receptor-like receptor (ORL1)and its endogenous peptide agonist nociceptin/orphaninFo (N/OFQ) during mouse embryogenesis have been investigated. Transcripts of ORL1 and N/OFQ were detected by RT-PCR in mouse brain of day 8 embryo (E8)and the expression continued afterwards. Northern blotanalysis revealed abundant expression of ORL1 at postnatal day 1 (P1) and N/OFQ at E17 and P1 in the brain butnone was detected in other embryonic tissues. The presence of functional ORL1 in mouse embryonic brain wasalso confirmed by specific binding of [3H] N/OFQ (kd=1.3±0.5 nM and Bmax = 72±9 fmol/mg protein) as wellas by N/OFQ-stimulated G protein activation.

关键词： Opioid receptor-like receptor (ORL1) nociceptin/orphanin FQ (N/OFQ) mouse embryogenesis functional expression

Molecular characterization and functional expression of opioid receptor-libe1 receptor

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Cell Research 1997年第1期7卷 69-77页

作者： wu ya lan LU PU KUN LING JIAN ZHAO ZHI JIE CHENG lan MA GANG PEI(Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China)(Shanghai Medical University, Shanghai 200032, China) Shanghai Medical University Shanghai 200032 China

The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.

关键词： Opioid receptor-libe1 receptor (ORL1) nociceptin/orphanin FQ (N/OFQ) SK-N-SH cells extracellular acidification functional expression

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新型柴油机喷油规律测试系统的设计

农机化研究 2007年第6期29卷 203-204,207页

作者：吴亚兰唐娟兰欣山东建筑大学机电学院济南250101 山东大学能源与动力工程学院济南250061

基于柴油机喷油器,设计了一种柴油机喷油规律测试系统。对喷油器进行改装设计,用以容纳、计量柴油机在喷油过程中喷出的油量,用位移传感器测量喷出油量的容积变化,通过数据处理得到喷油规律;设计了卸压装置,以防止测量系统因油压过高而... 详细信息

基于柴油机喷油器,设计了一种柴油机喷油规律测试系统。对喷油器进行改装设计,用以容纳、计量柴油机在喷油过程中喷出的油量,用位移传感器测量喷出油量的容积变化,通过数据处理得到喷油规律;设计了卸压装置,以防止测量系统因油压过高而受损。实验结果表明,该测试系统可以快捷地测量柴油机的喷油规律。

关键词：动力机械工程喷油规律测试系统设计喷油器

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“剂型”名词源流考释

中华中医药杂志 2019年第4期34卷 1487-1489页

作者：许霞吴亚兰安徽中医药大学合肥230012

"剂型"是医学和药学领域常用名词,是指将原料药加工制成适合于医疗或预防应用的形式。殷商朝就已有酒剂、汤剂等剂型的出现,从《五十二病方》始,历代众多医学书籍多记载有一些剂型。有关名词"成剂药"在《肘后备急方》中首次提出,《伤寒... 详细信息

"剂型"是医学和药学领域常用名词,是指将原料药加工制成适合于医疗或预防应用的形式。殷商朝就已有酒剂、汤剂等剂型的出现,从《五十二病方》始,历代众多医学书籍多记载有一些剂型。有关名词"成剂药"在《肘后备急方》中首次提出,《伤寒明理论·卷下·麻黄汤方》较早记载了"制剂"一词,与"剂型"术语内涵有别,但却包含了一定的剂型之意。《中医药的科学方向》中较早的记载了"剂型"一词。自"剂型"一词在中医药界使用以来,之后的著作、辞典、工具书及教科书类多有沿用,对后世有较大影响。

关键词：剂型源流考证

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改进柴油机螺旋撑簧油环性能的研究

内燃机学报 2006年第1期24卷 83-87页

作者：谢宗法曲云山吴亚兰张小印山东大学机械工程学院山东济南250061 山东轻工业学院机电工程系山东济南250100 山东建筑工程学院机电工程系山东济南250101

根据活塞环/缸套快速磨损模拟试验结果,发现螺旋撑簧油环的磨损量较大。该油环磨损后随开口间隙的增大,其接触比压迅速降低,严重影响了其刮油能力。通过对油环结构的改进,在开口间隙增量为2mm时,改进后的油环接触比压比原环提高45.7%;... 详细信息

根据活塞环/缸套快速磨损模拟试验结果,发现螺旋撑簧油环的磨损量较大。该油环磨损后随开口间隙的增大,其接触比压迅速降低,严重影响了其刮油能力。通过对油环结构的改进,在开口间隙增量为2mm时,改进后的油环接触比压比原环提高45.7%;通过快速模拟磨损试验发现铌铸铁油环耐磨性能比铬钼铜油环提高87.3%,并降低了油环的制造成本。装机使用说明,改进后的铌铸铁螺旋撑簧油环具有良好的使用性能。

关键词：柴油机油环快速模拟磨损试验切向弹力接触比压磨损性能

CaCl_2作为异硫氰酸曙红固体基质室温磷光增强剂及其在免疫分析中的应用

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高等学校化学学报 2005年第1期26卷 49-51页

作者：吴娅兰李隆弟刘佳铭朱国辉清华大学化学系北京100084 漳州师范学院化学系漳州363000

Room temperature phosphorescence(RTP) signal intensity of eosin-ITC on a polyamide membrane(PM) was very low, but the signal was increased obviously once some inorganic salts were added into the system at λ ex/λ em=532 nm/685 nm. The enhancement effect of ⅡA element ions(such as Ca 2+, Sr 2+ and Ba 2+) on RTP of eosin-ITC is much obvious than that of Pb 2+ and Hg 2+ used constantly as an outer heavy atom perturber in phosphorimetry. Based on that, a new solid substrate room temperature phosphorescence immunoassay(SS-RTP-IA) for the determination of the human IgG was proposed to demonstrate the feasibility using eosin-ITC as phosphorescent marker. It was found that a good linear relationship with the ΔI P was obtained when the amount of human IgG in the range from 6.25 to 400 pg/spot(corresponding to the mass concentration: 15.63 ng/mL—1 μg/L, sample volume of 0.4 μL/spot). The regression {equation} of working curve can be expressed as ΔI p=3.062+0.00864x(pg)(n=7, r=0.996), The detection limit calculated as 3S b/k is 0.394 pg/spot. The method was applied directly to the determination of IgG in human serum with a satisfying result. These results show that eosin-ITC can be used conveniently as a RTP marker of high sensitivity.

关键词：固体基质室温磷光免疫分析法异硫氰酸曙红 CaCl2作增强剂人IgG

Regioselective Synthesis of Novel Pyrazole Subunit Containing Isosteviol Derivatives

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Regioselective Synthesis of Novel Pyrazole Subunit Containin...

Tuning the interfacial reaction environment via pH-dependent and induced ions to understand C–N bonds coupling performance in NO3- integrated CO2 reduction to carbon and nitrogen compounds over dual Cu-based N-doped carbon catalyst

有机合成创新—产业化的新动力——中国化学会全国第三届有机合成化学与过程学术讨论会

作者： Liu,Congjun wu,ya Xu,Hao lan,Yubao Zhao,Hua Tao,Jingchao~* (Department of Chemistry,New Drug Research & Development center,Zhengzhou University,Zhengzhou 450052)

A large amount of interests have been raised in pyrazole derivatives that exhibit wide applications in the field of medicine,agricultural chemicals,perfume and so on,due to possessing a range of bioactivity including pesticidal, bactericidal,antimicrobial,antivirus,anticancer and other biological activities. In view of pyrazole subunit containing isosteviol derivatives(1,2) with high in vitro cytotoxic activities against mice melanoma cell lines(B 1 6-F1 0) that had been synthesized in our group,a series of novel isosteviol

关键词： isosteviol derivatives regioselective synthesis bioactivities

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Journal of Energy Chemistry 2025年第1期100卷 273-285页

作者： Tianhang Zhou Chen Shen Zhenghao wu Xingying lan Yi Xiao College of Carbon Neutrality Future Technology State Key Laboratory of Heavy Oil Processing China University of Petroleum (Beijing) Institute of Materials Science TU Darmstadt Department of Chemistry Xi'an Jiaotong-Liverpool University Department of Mechanical and Mechatronics Engineering and Waterloo Institute of Nanotechnology University of Waterloo Waterloo

Dual atomic catalysts （DAC）,particularly copper （Cu2）-based nitrogen （N） doped graphene,show great potential to effectively convert CO2and nitrate （NO3-） into important industrial chemicals such as ethylene,glycol,acetamide,and urea through an efficient catalytical process that involves C–C and C–N ***,the origin of the coupling activity remained unclear,which substantially hinders the rational design of Cu-based catalysts for the N-integrated CO2reduction reaction （CO2RR）.To address this challenge,this work performed advanced density functional theory calculations incorporating explicit solvation based on a Cu2-based N-doped carbon (Cu2N6C10) catalyst for *** calculations are aimed to gain insight into the reaction mechanisms for the synthesis of ethylene,acetamide,and urea via coupling in the interfacial reaction *** to the sluggishness of CO2,the formation of a solvation electric layer by anions （F-,Cl-,Br-,and I-） and cations (Na+,Mg2+,K+,and Ca2+) leads to electron transfer towards the Cu *** process significantly accelerates the reduction of *** results reveal that*CO intermediates play a pivotal role in N-integrated ***,the Cu2-based N-doped carbon catalyst examined in this study has demonstrated the most potential for C–N coupling to *** findings reveal that through the process of a condensation reaction between*CO and NH2OH for urea synthesis,*NO3-is reduced to*NH3,and*CO2to*CCO at dual Cu atom *** dual-site reduction facilitates the synthesis of acetamide through a nucleophilic reaction between NH3and the ketene ***,we found that the I-and Mg2+ions,influenced by pH,were highly effective for acetamide and ammonia synthesis,except when F-and Ca2+were ***,the mechanisms of C–N bond formation were investigated via ab-initio molecular dynamics simulations,and we found that adjusting the micro-environment can change the dominant side reaction,shift

关键词： pH-dependent micro-environments C–N coupling Dual Cu-based nitrogen carbon catalysts Explicit solvation model Ion stabilizer AIMD

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柴油机工作状态快速检测与故障诊断

拖拉机与农用运输车 2007年第1期34卷 87-89,92页

作者：吴亚兰唐娟兰欣郑希江山东建筑工程学院济南250014 山东大学济南250061

基于磁电传感器和高压油管夹持传感器,检测柴油机瞬时转速图谱和高压油管供油时的膨胀情况,对比柴油机各缸的压缩压力、做功能力及供油压力波形,结合柴油机的整机动力性,对柴油机工作状态进行不解体检测。本文介绍了该检测系统的检测原... 详细信息

基于磁电传感器和高压油管夹持传感器,检测柴油机瞬时转速图谱和高压油管供油时的膨胀情况,对比柴油机各缸的压缩压力、做功能力及供油压力波形,结合柴油机的整机动力性,对柴油机工作状态进行不解体检测。本文介绍了该检测系统的检测原理,及检测系统在柴油机现场性能检测中的应用。

关键词：柴油机状态检测瞬时转速

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6R机器人正运动学分析方法研究

机械设计与制造 2014年第3期 5-7,11页

作者：郑红梅邬亚兰合肥工业大学机械与汽车工程学院安徽合肥230009

6R机器人正运动学分析是指给定各关节的关节空间变量值,求解末端执行器在工作空间的位置和姿态。利用6R弧焊专用机器人的机械本体结构,在D-H坐标系和旋量理论坐标系下建立了末端执行器的运动学方程,利用MATLAB软件求解位姿矩阵;在虚拟... 详细信息

6R机器人正运动学分析是指给定各关节的关节空间变量值,求解末端执行器在工作空间的位置和姿态。利用6R弧焊专用机器人的机械本体结构,在D-H坐标系和旋量理论坐标系下建立了末端执行器的运动学方程,利用MATLAB软件求解位姿矩阵;在虚拟样机软件中建立了机器人运动学模型,进行运动学仿真得到末端执行器不同状态下的位移曲线。并将三种方法所得末端执行器的位姿进行了比较,验证了这三种方法的正确性。研究结果为后续的动力学分析、轨迹规划奠定了基础。

关键词： 6R机器人正运动学 D-H 旋量理论虚拟样机