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Nano Research 2024年第4期17卷 2388-2399页

作者： Jing Shao Yusheng Fang xiaobing wu Muhammad Imran Abdullah Youkun Tao College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060China School of Science Harbin Institute of TechnologyShenzhen 518055China

The sluggish kinetics of oxygen evolution reaction(OER)is the key tailback for hydrogen production from the water *** OER with thermodynamically auspicious methanol oxidation reaction(MOR)can significantly boost the H_(2) and value-added products ***,it is currently challenging to achieve a synergistic manipulation of product selectivity and performance for MOR ***,we report NiSnPH@OOH/CC(CC=carbon cloth)perovskite hydroxide nanosphere as an efficient MOR electrocatalyst with high activity,stability,and selectivity towards methanol oxidation to formate.A surface amorphous layer of defect rich NiOOH was generated in operando by selective Sn leaching with stable perovskite hydroxide bulk structure,which mitigates the oxidative power and optimizes the local coordination environment of the active NiOOH *** situ Raman combined with electrochemical studies further confirm the key active species,NiOOH,generated in operando enhance the MOR and blocking the over oxidation of methanol to CO_(2).As a result,NiSnPH@OOH/CC effectively masks the OER and attains>99%selectivity with 100%Faradic efficiency for *** results of this study show the advances of NiSnPH@OOH/CC as an efficient electrocatalyst for MOR and also suggest its potential applications for various small organic molecules oxidation.

关键词： perovskite hydroxide small organic molecules nickel oxy(hydroxide) value-added products methanol oxidation reaction

Mitogenomic analysis of the genus Panthera

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Science China(Life Sciences) 2011年第10期54卷 917-930页

作者： WEI Lei wu xiaobing ZHU LiXin JIANG ZhiGang Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources College of Life Sciences Anhui Normal University Wuhu 241000 China Faculty of Animal Science Suzhou Vocational Technology College Suzhou 234000 China Department of Chemistry and Life Sciences Chuzhou University Chuzhou 239000 China Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100080 China

The complete sequences of the mitochondrial DNA genomes of Panthera tigris,Panthera pardus,and Panthera uncia were determined using the polymerase chain reaction *** lengths of the complete mitochondrial DNA sequences of the three species were 16990,16964,and 16773 bp,*** of the three mitochondrial DNA genomes included 13 protein-coding genes,22 tRNA,two rRNA,one O L R,and one control *** structures of the genomes were highly similar to those of Felis catus,Acinonyx jubatus,and Neofelis *** phylogenies of the genus Panthera were inferred from two combined mitochondrial sequence data sets and the complete mitochondrial genome sequences,by MP (maximum parsimony),ML (maximum likelihood),and Bayesian *** results showed that Panthera was composed of Panthera leo,***,***,Panthera onca,***,and ***,which was included as the most basal *** phylogeny within Panthera genus was *** (*** (*** (***,(***,***)))).The divergence times for Panthera genus were estimated based on the ML branch lengths and four well-established calibration *** results showed that at about 11.3 MYA,the Panthera genus separated from other felid species and then evolved into the several species of the *** detail,*** was estimated to be founded about 8.66 MYA,*** about 6.55 MYA,*** about 4.63 MYA,and *** about 4.35 *** these estimated times were older than those estimated from the fossil *** divergence event,evolutionary process,speciation,and distribution pattern of ***,a species endemic to the central Asia with core habitats on the Qinghai-Tibetan Plateau and surrounding highlands,mostly correlated with the geological tectonic events and intensive climate shifts that happened at 8,3.6,2.5,and 1.7 MYA on the plateau during the late Cenozoic period.

关键词： Panthera uncia Panthera pardus Panthera tigris mtDNA phylogeny divergence time Qinghai-Tibetan Plateau

ISSR Markers as a Tool for Assessing Genetic Diversity in the Chinese Alligator (Alligator sinensis)

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Asian Herpetological Research 2012年第4期3卷 310-315页

作者： Chuanpeng NIE xiaobing wu Yanyan LI Juan ZHAO College of Life Sciences Anhui Normal University Zoology Practical Skills Training Center Fuyang Teachers College

Eight different inter simple sequence repeat(ISSR) markers were used as tools to investigate genetic variability and population differentiation in the Chinese alligator,Alligator sinensis,in this study. Eleven polymorphic bands(17.2%) out of a total of 64 were generated from 110 individuals in three populations. Analysis of molecular variation showed that most of the genetic variation(98.0%) occurred within the populations. Dendrogram relationship based on Nei’s unbiased genetic diversity illustrated that two breeding populations were genetically closely related. The Nm value of the study was 4.520,suggesting that high levels of gene flow existed and no differentiation appeared in the populations. In a reconstructed Neighbor-Joining tree,the haplotypes coming from the same populations did not gather as a class,suggesting the three populations had no apparent geographic pattern. This study shows that ISSR markers could be well applied as a feasible tool to assess genetic diversity in Chinese alligator individuals.

关键词： inter simple sequence repeat(ISSR) genetic diversity Chinese alligator endangered species

Sequence variability analysis on major histocompatibility complex class Ⅱ DRB alleles in three felines

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Frontiers in Biology 2008年第1期3卷 55-62页

作者： Qian WANG xiaobing wu Peng YAN Shuai ZHENG Anhui Key Laboratory of Conservation and Exploration for Important Biological Resources College of Life SciencesAnhui Normal UniversityWuhu 241000China Department of Histology and Embryology Wannan Medical CollegeWuhu 241001China Department of Clinical Medicine Wannan Medical CollegeWuhu 241001China

The variation of the exon 2 of the major histo-compatibility complex(MHC)class II gene DRB locus in three feline species were examined on clouded leopard(Neofelis nebulosa),leopard(Panthera pardus)and Amur tiger(Panthera tigris altaica).A pair of degenerated primers was used to amplify DRB locus covering almost the whole exon *** 2 encodes theβ1 domain which is the most vari-able fragments of the MHC class II ***-strand conformational polymorphism(SSCP)analysis was applied to detect different MHC class II DRB *** recombinant plasmids for each individual were screened out,isolated,purified and sequenced *** eight distinct haplotypes of exon 2 were obtained in four ***-in 237 bp nucleotide sequences from four samples,30 vari-able positions were found,and 21 putative peptide-binding positions were disclosed in 79 amino acid *** ratio of nonsynonymous substitutions(d_(N))was much higher than that of synonymous substitutions(d_(S)),which indicated that balancing selection probably maintain the variation of exon *** neighbor joining(NJ)and PAUP maximum parsimo-ny(MP)methods were used to reconstruct phylogenetic trees among species,*** displayed a more close relationship between leopard and tiger;however,clouded leopard has a comparatively distant relationship form the other two.

关键词： major histocompatibility complex(MHC) DRB locus exon 2 feline variability

Enhanced hydrogen production by insertional inactivation of adhE gene in Klebsiella oxytoca HP1

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Chinese Science Bulletin 2007年第4期52卷 492-496页

作者： ZHU JunBo LONG MinNan XU Fangcheng wu xiaobing XU HuiJuan Department of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China Bio-Energy Center School of Life Science Xiamen University Xiamen 361005 China

Ethanol is the main byproduct of anaerobic H2-producing fermentation in Klebsiella oxytoca HP1. Two moles of NAD(P)H are consumed to yield one mole of ethanol that may decrease bacterial hydrogen production. In this article the adhE gene that codes for acetaldehyde dehydrogenase was disrupted for the first time. A homologous recombination vector pTA-Str was constructed in which the adhE gene was disrupted by inserting an aminoglycoside-3'-adenyltransferase (aadA) gene. As expected, the vector includes the insertion 5′-adhE-aadA-adhE-3′. The amplified DNA fragment 5′-adhE-aadA-adhE-3′ from pTA-Str was transformed into K. oxytoca HP1 and one recombinant was obtained. PCR analysis of the resulting genomic DNA indicated the appropriate deletion and insertion. Compared with the H2-production of wild type K. oxytoca HP1, the hydrogen yield of the mutant increased by 16.07% and ethanol concentration decreased by 77.47%, suggesting that inactivation of the adhE gene in K. oxy- toca HP1 is a potential method for enhancing bacterial H2-production.

关键词：产酸克雷伯氏菌厌氧发酵氢气产量 adhE基因插入失活同源重组

The Impact of Stream Landscape on Genetic Structure and Dispersal Patterns in Stream Salamander(Pachyhynobius shangchengensis)

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Asian Herpetological Research 2020年第3期11卷 205-218页

作者： Tao PAN Hui WANG Peng YAN Chenling ZHANG Wenliang ZHOU xiaobing wu Baowei ZHANG School of Life Sciences Anhui UniversityHefei 230601AnhuiChina Anhui Province Key Laboratory of the Conservation and Exploitation of Biological Resource School of Life SciencesAnhui Normal UniversityWuhu 241000AnhuiChina Jiangsu Key Laboratory of Biofunctional Molecule Chemistry and Chemical Engineering School of Life SciencesJiangsu Second Normal UniversityNanjing 210013JiangsuChina

Compared with other terrestrial environments,the stream environment generally presents a linear spatial structure and relatively simple *** a stream landscape,the dispersal direction of stream-type organisms usually presents a linear structure along the stream,which results in the limited dispersal and the genetic differentiation of stream-type organisms across different stream *** Shangcheng Stout Salamander(Pachyhynobius shangchengensis)is a narrowly distributed stream salamander in Dabie Mountains of East *** the present study,we tested for the impact of stream landscape(*** and underground river)on genetic structure and dispersal pattern in *** based on 12 nuclear microsatellite loci from 195 individuals in 3 populations(A,B and C)from three closely connected sections within one *** diversity results suggested that Population B contains relatively high genetic diversity for *** when compared to the other populations(A and C).Detectable genetic differentiation was found(FST=0.008,P=0.007)among three populations,which was also supported by the Structure,FCA analysis and relatedness estimates of each pair of individuals among *** assignment test suggested that *** has philopatric males and female-biased dispersal(mean female Alc=.0.031,SE=0.225;mean male Alc=0.026,SE=0.198).Female-biased dispersal was also supported by analyses for each sex(*** autocorrelation,Genetic distance,Relatedness analysis).Our study indicated that small and isolated populations(A and C)had relatively low genetic diversity due to the limited population *** stream salamanders,landscape features(*** and underground river)can influence the ability of an individual to disperse through the landscape,and consequently influence the formation of strong genetic differentiation of ***.

关键词： Dabie Mounta ins genetic structure landscape features Pachyhynobius shangchengensis sexbiased dispersal

A MULTI-CRC SELECTIVE HARQ SCHEME FOR MIMO SYSTEMS

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Journal of Electronics(China) 2008年第1期25卷 53-58页

作者： Song Fei Tong xiaobing wu Qihui Wang Jinlong Institute of Communications Engineering PLA University of Science and Technology Nanjing 210007 China

A multi-Cyclic Redundancy Check (CRC) selective Hybrid Automatic-Repeat-reQuest (HARQ) scheme for improving the throughput efficiency of Multiple Input Multiple Output (MIMO) systems is proposed in this paper. According to different feedback information from the receiver, the proposed HARQ scheme employs two strategies, referred to as retransmission frame selection and space diversity. These two strategies decrease the successive frame errors upon retransmission. Theoretic analysis and computer simulation results show that this HARQ scheme achieves higher throughput than the existing HARQ schemes even in poor conditions of low Signal-to-Noise Ratio (SNR).

关键词： Hybrid Automatic-Repeat-reQuest () Multiple Input Multiple Output (MIMO) Cyclic Redundancy Check (CRC) Space diversity

STUDY ON PREPARATION OF REACTION BURNING SILICON CARBIDE BY CARBON POWDER

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Journal of wuhan University of Technology(Materials Science) 1997年第3期12卷 35-40页

作者：武七德 wuhan University of Technology China Institute of Mater. Sci. and Tech. Wuhan Univ. of Tech. Wuhan 430070 China

The technology of preparing reaction burning silicon carbide (RBSC) by replacing SiC/C with entirely carbonaceous raw materials is investigated. Experimental results show the predominant factors of successfully preparing RBSC are as following:strictly controlling the porosity and pore diameter of biscuit, obtaining ideal carbon network permeating of Si and completely reaction between Si and beta-SiC.

关键词： silicon carbide reaction burning carbonaceous raw material biscuit structure

A novel method for purification of recombinant adeno-associated virus vectors on a large scale

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Chinese Science Bulletin 2001年第6期46卷 485-489页

作者： wu xiaobing DONG Xiaoyan wu Zhijian CAO Hui NIU Dongbin QU Jianguo WANG Hong HOU Yunde State Key Laboratory for Molecular Virology and Genetic Engineering Beijing 100052China Laboratory of Morphology Institute of VirologyChinese Academy of Preventive MedicineBeijing 100052China

A novel method for recombinant adeno-associ-ated virus (rAAV) purification on large scale is described. The method involves three steps, including chloroform treatment, PEG/NaCl precipitation and chloroform extraction. The whole procedure can be performed in four hours. Using this purification method, we can reproducibly obtain, from 4 × 109 of proviral cells cultured in roller bottles, purified rAAV-GFP stocks with titers of around 5×1013 particles/mL and purity greater than 95%. The infectious titers of the vector stocks were up to 2×1012 TU/mL, thus particle-to-infectivity rate was about 25. Under an electronic microscope, most rAAV particles appeared full and a few were in intermediate form. Empty particles were rarely seen. The purified rAAV-GFP stocks have been successfully used in in vitro and in vivo transfection experiments. Therefore, this new method offers a simple, rapid and cost-effective way for large-scale rAAV purification.

关键词： recombinant adeno-associated virus chloroform purifi- cation.

Nest-site Use by the Chinese Alligator (Alligator sinensis) in the Gaojingmiao Breeding Farm,Anhui,China

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Asian Herpetological Research 2011年第1期2卷 36-40页

作者： Jianjun WANG xiaobing wu Dawei TIAN Jialong ZHU Renping WANG Chaolin WANG Anhui Provincial Key Laboratory for Conservation and Exploitation of Biological Resources College of Life SciencesAnhui Normal University Anhui Research Center for Chinese Alligator Reproduction

Nest-site and nesting material used by the Chinese alligator （Alligator sinensis） was studied at the Gaojingmiao Breeding Farm, Langxi County, Anhui, China from May to September 2009. In this study, artificial nesting materials were placed in 43 potential nesting sites before the nesting season, 11 of which were used. Additionally, eight nests were built at natural sites without artificial nesting materials provided. Seven environmental variables were measured at each nest site： distance from water, height from water surface, sunlight duration, nearest bank slope, nest site slope, vegetation coverage and concealment. Statistical analyses indicated that concealment was significantly different between used and unused nest sites, with concealment being significantly correlated to the use of materials- placed sites. In comparing the nests at artificial vs. natural sites, only the nearest bank slope differed significantly. Further, principal component analysis of natural nests indicated that the duration of nest exposure to sunlight and vegetation coverage were more influential than the other factors studied.

关键词： Chinese alligator nesting artificial nest natural nest habitat use reintroduction