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Genetic mapping of quantitative trait loci for the stigma exsertion rate in rice(Oryza sativa L.)

Journal of Integrative Agriculture 2017年第7期16卷 1423-1431页

作者： Md Habibur Rahman ZHANG Ying-xin SUN Lian-ping ZHANG Ke-qin Md Sazzadur Rahman wu wei-xun ZHAN Xiao-deng CAO Li-yong CHENG Shi-hua Key Laboratory for Zhejiang Super Rice Research/National Center for Rice Improvement/China National Rice Research Institute Hangzhou 310006P.R.China Department of Agricultural Extension Ministry of AgricultureDhaka 1215Bangladesh Plant Physiology Division Bangladesh Rice Research InstituteGazipur 1701Bangladesh

The efficiency of hybrid rice seed production can be improved by increasing the percentage of exserted stigmas. To identify quantitative trait loci （QTLs） for this trait, we conducted QTL mapping using 75 chromosome segment substitution lines （CSSLs） developed from a cross between the donor parent, Xieqingzao B （XQZB）, a maintainer line which has high stigma exsertion and the recurrent parent, Zhonghui 9308 （ZH9308）, a restorer line which has low stigma exsertion. Atotal of nine QTLs （qSSE5, qSSE10, qSSE11, qDSE10, qDSE11, qTSE5, qTSE6, qTSE10, and qTSE11） for single stigma exsertion （ssE）, dual stigma exsertion （DSE） and total stigma exsertion （TSE） were assessed in two environments （Hainan and Zhejiang）. Six of these QTLs （qSSE10, qSSE11, qDSE10, qDSE11, qTSE10, and qTSE11） were found in both environ- ments, while one QTL （qTSE6） was found in only Hainan, and two QTLs （qSSE5 and qTSE5） were found in only Zhejiang. The qSSE10, qSSE11, qDSE10, qDSE11, qTSE6, qTSE10, and qTSE11 alleles, which are derived from the parent XQZB, exhibited a positive additive effect. In contrast, the qSSE5 and qTSE5 alleles, which are derived from the parent ZH9308, exhibited a negative additive effect. The SSE, DSE and TSE traits were significantly correlated with each other in an en- vironmentally dependent manner. These results indicated that the lines showing higher values for SSE were more likely to exhibit increased values for DSE, which would ultimately increase TSE. To evaluate the advantage of exserted stigmas for cross-pollination, single, dual and total stigma exsertion should be considered separately in future attempts at genetic improvement to achieve increased production of rice hybrid seeds. This study also provides information for fine mapping, gene cloning and particularly marker-assisted selection （MAS）, on the latter and with an emphasis the phenotypic effects and implications of the QTLs for practical use in hybrid rice breeding.

关键词： hybrid rice stigma exsertion rate QTL mapping

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时钟信号竞争型三值CMOS边沿触发器

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电子学报 2000年第9期28卷 126-127页

作者：吴训威韦健汪鹏君宁波大学电路与系统研究所宁波315211 浙江大学信电系杭州310027

本文利用时钟信号的竞争冒险现象 ,提出了CMOS时钟信号竞争型三值D型边沿触发器的逻辑设计 .通过PSPICE程序模拟 ,证实了该设计具有正确的逻辑功能 ,而且与传统的三值D型维持阻塞触发器相比 ,它具有更简单的结构和更低的功耗 .

关键词：多值逻辑边沿触发器竞争冒险 CMOS

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K-Domain Splicing Factor OsMADS1 Regulates Open Hull Male Sterility in Rice

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Rice science 2015年第5期22卷 207-216页

作者： SUN Lian-ping ZHANG Ying-xin ZHANG Pei-pei YANG Zheng-fu ZHAN Xiao-deng SHEN Xi-hong ZHANG Zhen-hua HU Xia XUAN Dan-dan wu wei-xun LI Zi-he CAO Li-yong CHENG Shi-hua National Center for Rice Improvement / Key Laboratory for Zhejiang Super Rice Research China National Rice Research Institute College of Agronomy Shenyang Agricultural University

We identified the rice floral organ development mutant, termed as open hull and male sterile 1 （ohms1）, from the progeny of the indica restorer line Zhonghui 8015 treated with 60Co γ-ray irradiation. The ohms1 mutant exhibited an open hull and lemma- and palea-like structure conversion between the anthers and stigma, which resulted in the ohms1 mutant spikelet showing‘tridentate lemma＇. The ohms1 mutant was entirely sterile but had 60%-70% fertile pollen. Genetic analysis and gene mapping showed that ohms1 was controlled by a single recessive gene, and the mutant gene was fine-mapped to a 42-kb interval on the short arm of chromosome 3 between markers KY2 and KY29. Sequence analysis of the four open reading frames in this region revealed that the mutant carried a single nucleotide transformation （A to G） at the last base of the fifth intron, which was likely corresponded to ohms1 phynotype, in an MIKC type MADS-box gene OsMADS1 （LOC_Os03g11614）. Enzyme digestion and cDNA sequencing further indicated that the variable splicing was responsible for the deletion of the sixth exon in ohms1, but no structural changes in the MADS domain or amino acid frame shifts appeared. Additionally, real-time fluorescent quantitative PCR analysis showed that the OsMADS1 expression level decreased significantly in the ohms1 mutant. The expression levels of rice flowering factors and floral glume development-related genes also changed significantly. These results demonstrate that OsMADS1 may play an important role in rice floral organ development, particularly in floral glume development and floret primordium differentiation.

关键词： rice open hull male sterile ohms1 gene mapping alternative splicing floral organ

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