检索结果-南通市图书馆

Science china(Life sciences) 2010年第3期53卷 385-397页

作者： wu si1 & liang peiji2 1institute of neuroscience,shanghai institutes for biological sciences,state key laboratory of neurobiology,chinese academy of sciences,shanghai 200031,china 2Department of Biomedical Engineering,shanghai Jiaotong University,shanghai 200240,china 1. institute of neuroscience Shanghai Institutes for Biological Sciences State Key Laboratory of Neurobiology Chinese Academy of Sciences Shanghai 200031 China2. Department of Biomedical Engineering Shanghai Jiaotong University Shanghai 200240 China

The ultimate goal of Computational neuroscience(CNS) is to use and develop mathematical models and approaches to elucidate brain *** is a young and highly multidisciplinary *** heavily interacts with experimental neuroscience and such other research areas as artificial intelligence,robotics,computer vision,information science and machine *** paper reviews the history of CNS in china,its current status and the prospects for its future *** of CNS research in china are also presented.

关键词： computational neuroscience experimental neuroscience artificial intelligence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fibroblast growth factor 7 acts as a modulator of nociception

Fibroblast growth factor 7 acts as a modulator of nociceptio...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hui LIU~1,Qing-Feng wu~1,Qiong WANG~2,Lan BAO~2,Xu ZHANG~1 1 institute of neuroscience and state key laboratory of neuroscience,2 institute of Biochemistry and Cell Biology and laboratory of Molecular Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences, shanghai 200031,china

＜正＞Fibroblast growth factor（FGF）7,which is also known as keratinocyte growth factor（KGF）,is a member of the FGF *** studies regard it as a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial ***,its potential functions in the nervous system are poorly *** found that FGF7,together with its high-affinity receptor FGFR2b,was expressed in both dorsal root ganglion（DRG）and spinal cord ***7 mRNA was consistently increased in DRGs after peripheral nerve injury and transiently up-regulated after intraplantar injection of complete Freund’s *** injection of recombinant human FGF7 exacerbated the formalin-induced hyperalgesia, while application with either the antibodies against FGF7 or recombinant FGF R2β（Ⅲb）Fc chimera alleviated inflammatory nociceptive ***,behavior tests and wide-dynamic range neuron recordings of FGF7 knockout mice showed a defect in nociceptive *** together,these results suggest that FGF7 may contribute to inflammatory pain sensation and play an important role in nociceptive transmission.

关键词： fibroblast growth factor 7 inflammatory pain nociceptive transmission

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Rab5 dependent membrane endocytosis is required for Semaphorin3A induced axon growth cone collapse

Rab5 dependent membrane endocytosis is required for Semaphor...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Kong-Yan wu~1,Ai-Li SHENG~1,Wen-Wen LIU~2,An-Qi GENG~1,Xing-Yu JIANG~2,Xiao-Bing YUAN~1, Zhen-Ge LUO~(1,*) 1 institute of neuroscience,state key laboratory of neuroscience,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 CAS key Lab for biological Effects of Nanomaterials and Nanosafety,National Center for NanoScience and Technology,Beijing 100190,china

＜正＞Guidance cues are critical for axon navigation and ultimately for the wiring of the neuro-circuits during nervous system *** cones of growing axons can sense both attractive and repulsive cues in the environment and make a corresponding ***3A,a negative guidance cues,can collapse growth cones of outgrowing axons,however,the intracellular molecular mechanisms are not well *** we show that the small GTPase Rab5 is involved in growth one collapse in response to ***5 activity assay shows that Rab5 is transiently activated after treatment with *** cone collapse is abolished by down-regulation of Rab5 by specific siRNA in response to *** advantage of live cell FRET assay,we demonstrate that semaphorin3A can activate Rab5 and promote the retrograde trafficking of Rab5 positive endosomes in growth ***5 is required for endocytosis of the growth cone plasma membrane during growth cone collapse. Over-expression of Rab5 leads to axon growth cones collapse in vitro and impedes projecting axons growth in vivo. These findings indicate that in response to negative guidance molecules semaphorin3A,axon growth cones driving endocytosis of the plasma membrane associated with the activated function of Rab5 resulted in growth cone collapse.

关键词： growth cone Semaphorin3A Rab5 endocytosis plasma membrane FRET microfluidic culture system electroporation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Lipid rafts mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X3 receptor

Lipid rafts mediates ATP-induced endocytosis,signal transduc...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xu-Qiao CHEN~(1,#),Bin WANG~(1,#),Chengbiao wu~2,Jin PAN~1,Bo YUAN~3,Yuan-Yuan SU~1,Xing-Yu JIANG~3,Xu ZHANG~4 Lan BAO~(1,*) 1 laboratory of Molecular Cell Biology,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 Department of neurosciences,School of Medicine,University of California,San Diego,La Jolla,California,USA 3National Center of Nanoscience and Technology,Beijing 100190,china 4 institute of neuroscience and state key laboratory of neuroscience,shanghai institutes for biological sciences, chinese academy of sciences,shanghai 200031,china

P2X receptors are a family of cation channels expressed in neurons and nonneuronal *** play a variety of roles including synaptic transmission,muscle contraction,neuronal secretion and inflammation response. Among P2X receptors,the P2X subtype is selectively expressed in dorsal root ganglion *** raft is a specialized membrane microdomain and participate many signal transduction *** report showed that P2X receptor is localized in the lipid rafts of DRG *** have found P2X receptor engaged in retrograde transport in the form of signaling *** we investigated the role of lipid rafts in a,B-MeATP-dependent endocytosis and signal transduction,and retrograde transport of P2X *** of lipid raft with MBCD or nystatin abolished ATP-dependent endocytosis of P2X receptor and ATP-induced ERK activation in P2X receptor-expressing HEK293 *** vivo sucrose gradient fractionation with vesicular pellets prepared from sciatic nerve axoplasm showed that abundant P2X receptor was localized in lipid raft *** transported P2X receptors were colocalized with the lipid raft marker caveolinla in sciatic *** terminal treatment with MPCD attenuated retrograde transport of P2X receptor in axon of dorsal root ganglion ***,lipid raft mediates ATP-induced endocytosis,signal transduction and retrograde transport of P2X receptor.

关键词： P2X3 receptor lipid raft endocytosis ERK retrograde transport

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

ATP-dependent retrograde axonal transport of P2X3 receptor in primary sensory neurons

ATP-dependent retrograde axonal transport of P2X3 receptor i...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Bin WANG~1,Xu-Qiao CHEN~1,Cheng-Biao wu~2,Jin PAN~1,Bo YUAN~3,Yuan-Yuan SU~1,Xing-Yu JIANG~3, Xu ZHANG~4,Lan BAO~(1,*) 1 laboratory of Molecular Cell Biology,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 2 Department of neurosciences,School of Medicine,University of California,San Diego,La Jolla,California 92093- 0662,USA 3 National Center for Nanoscience and Technology,Beijing 100080,china 4 institute of neuroscience and state key laboratory of neuroscience,institutes for biological sciences,chinese academy of sciences,shanghai 200031,china

Objective P2X receptors are ATP-gated cation channels and play variable roles in pathophysiological *** P2X receptors,P2X

关键词： primary sensory neuron P2X3 receptor axonal retrograde transport

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A novel artemisinin derivative, 3-(12-β-artemisininoxy) phenoxyl succinic acid (SM735), mediates immunosuppressive effects in vitro and in vivo

引用

Acta Pharmacologica sinica 2005年第11期26卷 1352-1358页

作者： Wen-liang ZHOU~2, Jin-ming wu~3, Qing-li wu~2, Jun-xia WANG~2, Yu ZHOU~2, Ru ZHOU~2, Pei-lan HE~2, Xiao-yu LI~2, Yi-fu YANG~2, Yu ZHANG~3, Ying LI~3, Jian-ping ZUO~(2,4) Laboratories of ~2 Immunopharmacology and ~3 Synthetic Chemistry, Graduate School of the chinese academy of sciences, state key laboratory of Drug Research, shanghai institute of Materia Medica, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 201203, china Laboratorie of Immunopharmacology Graduate School of the Chinese Academy of Sciences State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China Laboratorie of Synthetic Chemistry Graduate School of the Chinese Academy of Sciences State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Aim: To study the immunosuppressive activity of SM735 {[3-(12-β-artemisininoxy)] phenoxyl succinic acid}, a synthetic artemisinin derivative with nonsteroidal antiinflammatory drug structure, with the aim of finding potential immunosuppressive agents. Methods: Concanavalin A (ConA), lipopolysaccharide (LPS), and mixed lymphocyte reaction (MLR), were used to induce the proliferation of splenocytes, and [~3H]-thymidine incorporation was used to evaluate the proliferation of splenocytes. Cytokine production was promoted with ConA, LPS, or PMA plus ionomycin, and was detected with the enzyme-linked immunosorbent assay. Dinitrofluorobenzene (DNFB) and sheep red blood cells (SRBC) were used to induce delayed-type hypersensitivity and quantitative hemolysis of SRBC (QHS) mouse models, as criteria for the evaluation of in vivo immune activity. Results: SM735 strongly inhibited the proliferation of splenocytes induced by ConA, LPS, or MLR, withIC50) values of 0.33 μmol/L, 0.27 μmol/L, and 0.51 μmol/L, respectively. When compared with a CC50value of 53.1 μmol/L, SM735 had a favorable safety range. SM735 dose-dependently inhibited proinflammatory cytokine production [including interleukins (IL)-12, interferon (IFN)-γ and IL-6] induced by LPS or PMA plus ionomycin. Upon ConA stimulation, SM735 suppressed IFN-γ in a dose-dependent manner, but did not affect IL-2 secretion. SM735 also strongly suppressed both T-cell-mediated delayed-type hypersensitivity (DTH) and B-cell-mediated QHS reactions. Conclusion: SM735 had strong immunosuppressive activity in vitro and in vivo, suggesting a potential role for SM735 as an immunosuppressive agent, and established the groundwork for further research on SM735.

关键词： artemisinin non-steroidal anti-inflammatory agents SM735 immuno-suppressive activity

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cation-chloride co-transportors involved in cyclothiazide induced depolarizing shift of EGABA in CA1 pyramid neurons during seizure

Cation-chloride co-transportors involved in cyclothiazide in...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Zheng wu Yun WANG~* institutes of Brain Science and state key laboratory for Medical neurobiology Fudan University shanghai 200032 china

＜正＞Imbalance between inhibitory and excitatory in the brain is one of the basic mechanisms for epileptogenesis. Previous study from our lab has shown cyclothiazide（CTZ） is a potant convulsant in inducing epilepsy both in vitro and in vivo by activating glutamatergic and suppressing GABAergic ***-R-induced fast response depends on the concentration gradient of intracellular and extracellular chloridion,which is regulated partly by cation-chloride cotransportors （CCCs）.The aim of current study is to investigate the functional role of CCCs in CTZ induced epileptogenesis. Rat hippocampal slice（P21-P30） were incubated with 50μM CTZ for 2 hours before CA1 pyramid neurons were recorded using whole cell patch *** induced robust spontaneous discharges to epileptiform bursts in CA1 pyramid neurons and a depolarizing shift in EGABA（control = -62.56±1.06 mV,n = 14 vs CTZ = -56.15±0.93 mV,n=l 1;P＜0.05）.To determine the contributions of CCCs in setting EGABA under these conditions,furosemide,a CCCs inhibitor,was tested on its effect on *** with previous reports,furosemide（100μM） also induced a depolarizing shift in EGABA（Furosemide = -57.58±1.51 mV,n = 5）.In conclusion,our results demonstrate that down-regulation of CCCs activity may contribute to the CTZ-induced epileptiform activity in CA1 pyramid neurons.

关键词： epilepsy EGABA shift cyclothiazide hippocampus KCC2

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Development of starburst amacrine cells in the mouse retina

Development of starburst amacrine cells in the mouse retina

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Rong-Yu LIAO Hai-Tian liang Shi-Gang HE~* state key laboratory of Brain and Cognitive sciences institute of Biophysics chinese academy of sciences Beijing 100101 china

＜正＞Objective A type of interneuron,starburst amacrine cells（SACs） is discovered in the rabbit *** are the only neurons in the retina known to synthesize both ACh and GABA and provide critical input to direction selective ganglion *** present study investigated the morphological changes of SACs in developing mouse *** （1） The soma of SACs in the developing mouse retina was stained with anti-ChAT antibody.（2） Vertical sections of mouse retina were stained with ChAT and VAChT（3） The morphology of SACs at different developmental stages（PO,P3,P5,P8, P13,adult） was labeled with ***（1） The dendritic field of SACs predicted based on increasing eyeball diameter was bigger than the measurement.（2） The coverage factor increased as the retina developed.（3） The branching point index and pixel area index of SACs underwent an increase along with the development of ***（1） The dendrites of SACs grew faster than retina during P0-P13 but retracted after P13.（2） The dendritic inner part of SACs became simpler when the retina developed.

关键词： retina direction-selective ganglion cell(DSGCs) starburst amacrine cell(SAC) coverage factor branching point index pixel area index.

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Ca2+-dependent suppression of synaptic transmission by presynaptic CB1 receptors in rat retinal ganglion cells

Ca2+-dependent suppression of synaptic transmission by presy...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Xiao-Han WANG,Yi wu,Xiong-Li YANG,Zhong-Feng WANG~* institute of neurobiology,institutes of Brain Science and state key laboratory of Medical neurobiology,Fudan University,shanghai 200032,china

Previous work has demonstrated that endocannabinoid serves as a retrograde signaling to regulate neurotransmitter release via activating presynaptic CB 1 receptors in the CNS,which plays a critical role in neural development, synaptic plasticity,learning and memory,*** CB 1 receptors are widely expressed in amacrine cells,bipolar cells and the inner plexiform layer in the retina,functions of endocannabinoid are still *** the present work,we explored modulation by cannabinoid of glycine receptor-mediated miniature inhibitory postsynaptic currents(mIPSCs) and AMPA receptor-mediated miniature excitatory postsynaptic currents(mEPSCs) in retinal ganglion cells(RGCs) by patch-clamp techniques in rat retinal *** normal 2 mM Ca-containing bath solution,external application of 5μM WIN55212,a CB1 receptor agonist,significantly suppressed the frequency of *** effect was reversed by co- application of 10μM AM251,a CB1 receptor antagonist,indicating that the effect was mediated by CB1 receptors localized in the terminal of glycinergic amacrine cell presynaptic to *** contrast,WIN55212 failed to affect the frequency of mEPSCs in the same *** extracellular Ca([Ca]o)-free condition,in which the frequency of mIPSCs was significantly reduced,WIN55212 persisted to suppress the frequency of ***[Ca]o was elevated from 2 mM to 5 mM,the frequencies of both mIPSCs and mEPSCs were increased ***,under higher[Ca]o, WIN55212 dramatically inhibited the enhanced frequencies of both mIPSCs and ***-application of AM251 reversed the WIN55212-induced *** results suggest that cannabinoid signaling suppresses synaptic transmission in rat RGCs in a Ca-dependent *** regulation of inhibitory and excitatory synaptic transmission by cannabinoid signaling suggests versatile functions of this signaling in the retina.

关键词： CB1 receptor mIPSC mEPSC retinal ganglion cell

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Voltage-sensitive dye imaging of dysgranular cortex activation evoked by ipsilateral whisker stimulation

Voltage-sensitive dye imaging of dysgranular cortex activati...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Zhi-Jie WANG~1,Xin GAO~1,Bing LI~(1,*),Jian-Young wu~(2,*) 1 state key laboratory of Brain and Cognitive Science,institute of Biophysics,chinese academy of sciences,Beijing 100101,china 2 Department of Physiology and Biophysics,Georgetown University,Washington,DC 20057,USA

＜正＞Integration of bilateral sensory signals is important for the survival of an *** primary somatosensory cortex（si） can be activated by both contralateral and ipsilateral whisker stimulation,though it does not receive directly ipsilateral sensory *** has been proposed that the activity evoked by ipsilateral whisker stimulation is transmitted from contralateral si,but the pathway for the transmission remains *** use voltage-sensitive dye imaging to visualize the whisker-evoked responses in rat somatosensory cortex and surrounding *** find that ipsilateral whisker stimulation activates first the dysgranular zones（DZ） outside the barrel cortex,and the evoked activity forms a propagating wave that spreads into the whisker sub-barrel *** stimulation to the contralateral si further confirms the *** initiation sites in the DZ activation are close to each other when different ipsilateral whiskers are stimulated.A comparison between the responses to contralateral and bilateral stimulation indicates that the involvement of DZ activation may improve the discriminability of the *** results suggest that ipsilateral whisker stimulation first initiates activation in the corresponding barrel in the contralateral side,and activates the ipsilateral DZ via the interhemispheric connections between the DZ *** ipsilateral somatosensory activation has a non-specific representation in the DZ and does not overlap with that of contralateral whisker *** activation may participate in the sensory processing,gain control,and bilateral sensory integration.

关键词： dysgranular zones primary somatosensory cortex interhemispheric connections ispilateral whisker stimulation voltage-sensitive dye imaging

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：