检索结果-南通市图书馆

Acta Oceanologica Sinica 2006年第4期25卷 91-98页

作者： CHEN Shixi HONG Wanshu ZHANG Qiyong wu renxie WANG Qiong State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China Department of Oceanography Xiamen University Xiamen 361005 China

The rates of oxygen consumption, tolerance of hypoxia and desiccation of the Chinese black sleeper （ Bostrichthys sinensis） and mudskipper （Boleophthalmus pectinirostris） embryos were investigated. The pattern of oxygen consumption of the Chinese black sleeper embryos was similar to that of the mudskipper ones. The lowest rates of oxygen consumption [ （ 1.65 ±0. 66） nmol/ （ind. ·h） ] of the Chinese black sleeper embryos 16 h after fertilization and the lowest rates of oxygen consumption [ （0.79± 0.08 ）nmolf（ ind. · h） ] of the mudskipper embryos 6 h after fertilization were recorded, respectively. Then the rates of oxygen consumption of these two species embryos increased gradually until hatching [ （8.26 ± 1.70 ） nmolf（ ind.· h） in the Chinese black sleeper, （2.69 ± 0.23 ）nmolf（ ind. · h） in mudskipper]. After exposure to hypoxia water （0.16 mg/dm^3）, bradycardia of the embryos occurred in both the Chinese black sleeper and the mudskipper. However, the Chinese black sleeper embryos survived approximately 45 min longer than the mudskipper ones. After exposure to desiccation at a relative humidity of 58%, bradycardia of the embryos was observed in both the Chinese black sleeper and the mudskipper, and the Chinese black sleeper embryos lived approximately 9 min longer than the mudskipper ones.

关键词： Chinese black sleeper (Bostrichthys sinensis) mudskipper (Boleophthalmus pectinirostris) embryo oxygen consumption rate hypoxia tolerance desiccation tolerance

Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq, and cross-amplifi cation to related species

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2019年第1期37卷 245-255页

作者： NIU Sufang ZHAI Yun wu renxie ZHANG Haoran TIAN Letian DENG Jiaxin XIAO Yao College of Fisheries Guangdong Ocean University

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this ?shery resources. The recently developed speci?c-locus ampli?ed fragment sequencing(SLAF-seq) is an effcient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites(mono-to hexa-nucleotide repeats) were identi?ed using SLAF-seq technology, of which di-nucleotide was the most frequent(13 590, 47.02%), followed by mono-nucleotide(8 138, 28.15%), tri-nucleotide(5 727, 19.81%), tetra-nucleotide(1 104, 3.82%), pentanucleotide(234, 0.81%), and hexa-nucleotide(112, 0.39%). One hundred and thirty-two microsatellite loci(di-and tri-nucleotide) were randomly selected for ampli?cation and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity( Ho) and expected heterozygosity( He) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and0.836, respectively. The polymorphism information content(PIC) ranged from 0.341 to 0.941(mean=0.806).However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in ?ve phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-ampli?ed in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides *** present microsatellites provided the ?rst known set of microsatellite DNA markers for D. maruadsi,D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the ?sheries man

关键词： Decapterus maruadsi SLAF-seq microsatellite markers cross-amplification

DNA barcoding of the family Sparidae along the coast of China and revelation of potential cryptic diversity in the IndoWest Pacific oceans based on COI and 16S rRNA genes

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2018年第5期36卷 1753-1770页

作者： wu renxie ZHANG Haoran LIU Jing NIU Sufang XIAO Yao CHEN Yongxia College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China Laboratory of Marine Organism Taxonomy and Phylogeny Institute of OceanologyChinese Academy of SciencesQingdao 266071China College of Life Sciences Hebei UniversityBaoding 071002China

Sparids are of considerable economic importance in marine fishery and aquaculture in China, and the species diversity of this group is considered relatively high. However, the accurate species identification and delimitation of sparids in China remain unaddressed. In this study, we used mitochondrial cytochrome oxidase subunit ?(COI) and 16S ribosomal RNA(16S) genes to conduct DNA barcoding and species delimitation in eleven sparid species from the coastal waters of China. Based on Kimura-2 parameter genetic distances, the mean intraspecific/interspecific variation for COI and 16S were calculated as 0.004/0.152 and 0.002/0.072, respectively. All the conspecific individuals formed monophyletic clusters in neighbour-joining trees of both markers. An obvious barcoding gap was detected for each species, and a common genetic threshold of 1.3% sequence divergence was defined for species delimitation in both markers. Although the sequence variation of 16S was generally lower than that of COI, the results indicated that sparid species could be ef fectively and accurately identified and delimited by COI as well as 16S. Thus, we propose that the COI gene serve as the standard DNA barcode for sparids, and that the 16S gene could also be an ideal candidate barcode. Moreover, each of the six sparid species( Argyrops spinifer, Rhabdosargus sarba, Dentex hypselosomus, Acanthopagrus latus, Acanthopagrus australis and Acanthopagrus berda) showed high intraspecific divergence(>1.3% genetic threshold) with the remarkable geographic lineages in the Indo-West Pacific oceans, which supported that potential unrecognized cryptic species were in them. The potential cryptic diversity revealed here might be primarily attributed to the allopatric divergences caused by the long-term geographic isolation between the Indian and West Pacific oceans or between the opposite sides of the Indian Ocean. The results further suggest that a revision of taxonomic status of these species is required, follow

关键词： SpaJcidae DNA barcoding species delimitation cryptic species Chinese coast Indo-West Pacific

Population structure and genome‑wide evolutionary signatures reveal putative climate‑driven habitat change and local adaptation in the large yellow croaker

在线全文

学校读者我要写书评

暂无评论

Marine Life Science & Technology 2023年第2期5卷 141-154页

作者： Baohua Chen Yulin Bai Jiaying Wang Qiaozhen Ke Zhixiong Zhou Tao Zhou Ying Pan renxie wu Xiongfei wu Weiqiang Zheng Peng Xu Fujian Key Laboratory of Genetics and Breeding of Marine Organisms College of Ocean and Earth SciencesXiamen UniversityXiamen 361102China National Key Laboratory of Mariculture Breeding Ningde Fufa Fisheries Company LimitedNingde 352000China State Key Laboratory of Marine Environmental Science Xiamen UniversityXiamen 361102China Institute of Biotechnology Fujian Academy of Agricultural SciencesFuzhou 350000China College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China Ningbo Academy of Oceanology and Fishery Ningbo 315012China

The large yellow croaker(Larimichthys crocea)is one of the most economically valuable marine fsh in China and is a notable species in ecological studies owing to a serious collapse of wild germplasm in the past few *** stock division and species distribution,which have important implications for ecological protection,germplasm recovery,and fshery resource management,have been debated since the ***,it is still uncertain even how many stocks exist in this *** address this,we evaluated the fne-scale genetic structure of large yellow croaker populations distributed along the eastern and southern Chinese coastline based on 7.64 million SNP *** with the widely accepted stock boundaries proposed in the 1960s,our results revealed that a climate-driven habitat change probably occurred between the Naozhou(Nanhai)Stock and the Ming-Yuedong(Mindong)*** boundary between these two stocks might have shifted northwards from the Pearl River Estuary to the northern area of the Taiwan Strait,accompanied by highly asymmetric *** addition,we found divergent landscapes of natural selection between the stocks inhabiting northern and southern *** northern population exhibited highly agminated signatures of strong natural selection in genes related to developmental processes,whereas moderate and interspersed selective signatures were detected in many immune-related genes in the southern *** fndings establish the stock status and genome-wide evolutionary landscapes of large yellow croaker,providing a basis for conservation,fsheries management and further evolutionary biology studies.

关键词： Climate adaptation Habitat change Large yellow croaker Phylogeographic structure

珠带鱼和带鱼未定种Trichiurus sp.2的分子系统进化关系

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国水产科学 2012年第3期19卷 373-380页

作者：郭刘军吴仁协刘静广东海洋大学水产学院中国科学院海洋研究所

针对南海大型带鱼新种——珠带鱼(Trichiurus margarites Li,1992)与日本产带鱼未定种***.2(sensu Nakabo,2002)之间的系统进化关系及分类地位不明确问题,本研究通过测定采自海南岛西岸珠带鱼的线粒体16S rRNA基因部分序列,结合已报道的... 详细信息

针对南海大型带鱼新种——珠带鱼(Trichiurus margarites Li,1992)与日本产带鱼未定种***.2(sensu Nakabo,2002)之间的系统进化关系及分类地位不明确问题,本研究通过测定采自海南岛西岸珠带鱼的线粒体16S rRNA基因部分序列,结合已报道的***.2及其近缘种的同源序列,对其16S rRNA基因序列变异进行分析。基于Kimura双参数模型计算,海南岛西岸珠带鱼与日本九州、海南岛东岸及印度洋***.2间的净遗传距离(0.000～0.006)远低于属内其他种间的净遗传距离(0.037～0.061),表明二者的遗传分化程度处于种内水平。邻接法(NJ)和最大似然法(ML)构建的分子系统树显示,珠带鱼与***.2以极高的置信度(NJ 100%,ML 96%)聚为一支单系,并与其他带鱼形成姐妹群。根据所得分子数据并结合形态学研究资料,可以确定日本产带鱼未定种即是珠带鱼,它广泛分布于印度西太平洋。综合3种带鱼属鱼类在西北太平洋的种间分化事件,对珠带鱼的起源及演化进行了初步推测。

关键词：珠带鱼带鱼未定种 16S rRNA序列系统进化分类地位

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国鲷科鱼类分类和系统发育研究进展

中国水产科学 2011年第2期18卷 472-480页

作者：樊冀蓉吴仁协赵元莙刘静3 重庆师范大学重庆市动物生物学重点实验室广东海洋大学水产学院中国科学院海洋研究所海洋生物分类与系统演化实验室

鲷科(Sparidae)隶属于硬骨鱼纲(Osteichthyes)、鲈形目(Perciformes)、鲈亚目(Percoidei)。目前已报道世界有34属133种,广泛分布于大西洋、印度洋和太平洋的温带至热带水域,在世界海洋渔业中占有重要地位。虽然此类群系统学研究已有250... 详细信息

鲷科(Sparidae)隶属于硬骨鱼纲(Osteichthyes)、鲈形目(Perciformes)、鲈亚目(Percoidei)。目前已报道世界有34属133种,广泛分布于大西洋、印度洋和太平洋的温带至热带水域,在世界海洋渔业中占有重要地位。虽然此类群系统学研究已有250多年历史,但是在亚科、属、种(亚种)等不同分类阶元上仍然存在颇多争议和疑问,有必要对其进行深入研究。本文总结了近百年来有关鲷科鱼类的分类和系统发育研究的文献资料,厘定了中国鲷科鱼类的有效物种和种名,修订了过去文献中出现的主要同物异名和错误的命名种,初步确认中国鲷科鱼类现有7属18种(或亚种);在探讨鲷科鱼类分类地位的基础上,指出了当前中国鲷科鱼类系统分类研究中存在的问题,展望了今后研究的方向,旨在为深入研究鲷科鱼类以合理保护和利用其资源提供参考依据。

关键词：鲷科分类系统发育

采用高通量技术开发花鲈二碱基重复微卫星标记

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

基因组学与应用生物学 2020年第2期39卷 507-513页

作者：翟云吴仁协牛素芳沈榕张浩冉广东海洋大学水产学院湛江524088

花鲈是中国重要的捕捞和养殖对象之一。本研究利用高通量测序法开发花鲈微卫星标记,共获得60632个二至六碱基重复微卫星序列,从52747个二碱基重复序列中随机挑选150个位点进行引物合成及多态性检测,最终开发出27个具有多态性的微卫星标... 详细信息

花鲈是中国重要的捕捞和养殖对象之一。本研究利用高通量测序法开发花鲈微卫星标记,共获得60632个二至六碱基重复微卫星序列,从52747个二碱基重复序列中随机挑选150个位点进行引物合成及多态性检测,最终开发出27个具有多态性的微卫星标记。群体遗传学检测结果显示,27个微卫星标记的等位基因数为6~22(均值12.667),观测杂合度(Ho)为0.323~1.000(均值0.614),期望杂合度(He)为0.723~0.934(均值0.861),多态信息含量(PIC)为0.665~0.915(均值0.830),各位点PIC值均大于0.500,表明所开发的二碱基重复微卫星标记均具有较高的多态性。哈迪-温伯格平衡(HWE)检测结果显示,14个标记符合HWE,其中LM2-11与LM2-2、LM2-16之间存在连锁不平衡(p<0.050),其他符合HWE的标记间不存在连锁不平衡。Wilcoxon检验结果显示,花鲈群体并未偏离L-shaped分布,表明其经历过近期的遗传瓶颈效应。本研究开发的27个微卫星位点可为花鲈的分子标记辅助育种、增殖放流遗传效应评估、群体遗传学等研究提供有效的分子标记。

关键词：花鲈高通量测序微卫星标记

石斑鱼转录因子NR1D1和NR1D2在冷胁迫中的调控作用

在线全文

学校读者我要写书评

暂无评论

应用海洋学学报 2024年第1期43卷 147-159页

作者：苗奔奔林楚曹文科梁燕珊吴仁协牛素芳梁镇邦广东海洋大学水产学院广东湛江524088 南方海洋科学与工程广东省实验室(湛江) 广东湛江524025

NR1D1和NR1D2转录因子属于核受体转录因子家族,二者通过调控靶基因表达参与众多生物学过程,尤其在昼夜节律调节中发挥重要作用。本研究通过分析NR1D1和NR1D2的蛋白质分子特征以及在冷胁迫下珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀... 详细信息

NR1D1和NR1D2转录因子属于核受体转录因子家族,二者通过调控靶基因表达参与众多生物学过程,尤其在昼夜节律调节中发挥重要作用。本研究通过分析NR1D1和NR1D2的蛋白质分子特征以及在冷胁迫下珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)的基因调控关系以及它们所参与的通路,揭示它们在鱼类低温环境中的功能作用。生物信息学比较分析显示NR1D1和NR1D2基因序列均有23个开放阅读框,编码的蛋白质均属于不稳定的亲水性蛋白质。NR1D1和NR1D2蛋白质均含有ZF-C4类锌脂蛋白结构域和Hormone_Recep核激素受体的配体结合域,主要配体为含铁原卟啉IX。这两种蛋白主要在细胞核中发挥调控基因表达的作用,且与昼夜节律通路的多个关键基因有直接作用关系。结果表明,在冷胁迫下NR1D1和NR1D2作为转录因子可通过调控靶基因的表达,参与昼夜节律调节并促进糖脂代谢、线粒体氧化等过程,为珍珠龙胆石斑鱼提供必要的能量,有助于增强其耐寒性。

关键词：海洋生物学珍珠龙胆石斑鱼冷胁迫核受体转录因子家族昼夜节律调节

基于SLAF-seq技术的沙带鱼(Lepturacanthus savala)微卫星标记开发以及在近缘种中的通用性

在线全文

学校读者我要写书评

暂无评论

基因组学与应用生物学 2018年第8期37卷 3331-3338页

作者：张浩冉梁镇邦吴仁协牛素芳梁炎王权韦欢肖瑶孙贝广东海洋大学水产学院

沙带鱼是带鱼科中一种重要的海洋经济鱼类,广泛分布于印度-西太平洋海域。近年来,过度捕捞和渔业生态环境变化使沙带鱼的自然资源和种群结构受到了严重影响。因此,为了制定出科学合理的资源保护和管理措施,急需开发出大量多态的沙带鱼... 详细信息

沙带鱼是带鱼科中一种重要的海洋经济鱼类,广泛分布于印度-西太平洋海域。近年来,过度捕捞和渔业生态环境变化使沙带鱼的自然资源和种群结构受到了严重影响。因此,为了制定出科学合理的资源保护和管理措施,急需开发出大量多态的沙带鱼微卫星位点以促进其种群遗传学研究。本研究采用SLAF-seq技术,首次在沙带鱼基因组DNA中筛选出28个多态性微卫星位点。每个位点的等位基因数为2~18,平均值9.11;观测杂合度和期望杂合度分别为0.077~0.871（平均值0.634）和0.151~0.942（平均值0.711）;各位点多态信息含量为0.137~0.919,平均值0.670。经过Bonferroni校正后,有3个位点显著偏离哈迪-温伯格平衡（HWE）,并显示出较高的近交系数和无效等位基因频率,其余25个位点均符合HWE。这些多态性微卫星DNA标记可为沙带鱼的种群遗传资源研究提供有效的分子标记和技术支撑。此外,跨物种扩增结果表明,有19个微卫星标记在4种带鱼属鱼类中具有较好的通用性,这些标记可为阐明带鱼科属间或种间的系统进化关系提供新的研究手段和视角。

关键词：沙带鱼微卫星标记高通量测序 SLAF-seq技术跨物种通用性

在线全文

学校读者我要写书评

暂无评论

大黄鱼野生和养殖群体的COI序列变异分析

广西科学 2023年第4期30卷 794-803页

作者：雷凤玲陈敏芳蒙扬鑫牛素芳吴仁协潘滢广东海洋大学水产学院广东湛江524088 宁德市富发水产有限公司大黄鱼育种国家重点实验室福建宁德352103

为阐明大黄鱼Larimichthys crocea野生和养殖群体的遗传变异特性,本研究采用COI基因序列对徐闻东岸野生群体(XWD)、硇洲岛野生群体(NZD)、闽粤东野生群体(MYD)、象山养殖群体(XSYZ)和宁德养殖群体(NDYZ)等5个大黄鱼群体的遗传多样性水... 详细信息

为阐明大黄鱼Larimichthys crocea野生和养殖群体的遗传变异特性,本研究采用COI基因序列对徐闻东岸野生群体(XWD)、硇洲岛野生群体(NZD)、闽粤东野生群体(MYD)、象山养殖群体(XSYZ)和宁德养殖群体(NDYZ)等5个大黄鱼群体的遗传多样性水平、单倍型分布、遗传分化、历史动态等进行比较分析。结果显示,3个野生群体的遗传多样性水平(h=0.757 6-0.877 5、π=0.002 6-0.003 1)明显高于2个养殖群体(h=0.194 7-0.495 2、π=0.000 8-0.001 0),二者的单倍型分布频率差异明显;XSYZ与NDYZ、MYD群体间存在显著的遗传分化(F_(st)=0.128 5、0.086 0,P0.05)。历史动态分析支持野生大黄鱼经历了晚更新世的群体数量扩张,但不支持养殖大黄鱼发生群体扩张。研究表明群体间遗传多样性的差异反映了不同进化力量在大黄鱼野生和养殖群体遗传中的作用机制。养殖实践和人工选育对东海区大黄鱼群体间的遗传分化起到了重要作用,而群体扩张、养殖逃逸、人工放流以及物种的扩散能力等因素可能促使粤西海域和东海区大黄鱼群体间具有遗传同质性。本研究揭示了粤西海域和东海区大黄鱼群体种质资源状况以及野生群体与养殖群体间的遗传特性差异,可为大黄鱼种质资源保护和利用提供科学依据。

关键词：大黄鱼 COI基因序列变异遗传分化种质资源

同方期刊数据库