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Effects of Different Preceding Crops on Soil Micro-ecological Environment and Yield of Cucumber

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Journal of Northeast Agricultural University(English Edition) 2011年第4期18卷 6-14页

作者： LIU Shouwei LIU Shuqin PAN Kai WANG lili wu Fengzhi College of Horticulture Northeast Agricultural University Harbin 150030 China

Pepper, celery, eggplant and tomato were used as preceding crops to study their effects on the yield, soil microorganism quantity and soil enzyme activities of cucumber. Results showed that four preceding crops all increased soil microorganism quantity in cucumber, but decreased population of Fusarium oxysporum. The effect of pepper was more significant than that of the others Populations of soil bacteria, fungi and actinomyces of pepper treatment were significantly higher than those of the other treatments, except that the populations of fungi had no significant difference with celery and eggplant treatments on 50 days after transplanting, while that of Fusarium oxysporum was fewer than that of the other treatments. The soil microorganism quantity in celery and eggplant treatment was more significant than tomato, but lower population of Fusarium oxysporum. Four preceding crops all increased sol enzyme activities, lnvertase and urease activities of pepper treatment were significantly higher than those of the other treatments, catalase activities of pepper, celery and eggplant treatments were significantly higher than those of tomato and the control treatments All preceding crops remarkably increased cucumber yield, with pepper as the highest. Comparing with the control, cucumber yields of pepper, celery and eggplant treatments increased by 24.9%, 13.6% and 11.9%, respectively. Results suggested that four preceding crops all improved soil microbial ecology and increased cucumber yield. The pepper was the most suitable preceding crop, then followed by celery and eggplant. Tomato had the similar effect as the control.

关键词： preceding crop cucumber yield soil microorganism soil enzyme

Construction of plant expression vector of Pseudopleuronectes americanus antifreeze protein gene

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Journal of Northeast Agricultural University(English Edition) 2007年第3期14卷 206-211页

作者： LI Shufeng ZHAO Wei YUAN lili wu Jiang YAN Yunqin College of Life Sciences Northeast Agricultural University Harbin 150030 China

The Pseudopleuronectes americanus antifreeze protein gene was synthesized and control sequences were added such as 35S promoter and nos terminator that can facilitate the transcription and Ω sequence and Kozak sequence that can improve the expression in translation level, the high expression cassette of antifreeze protein was constructed. This cassette was connected to pBI121.1 and finally got the high expression vector pBRTSAFP introduced into the maize callus. The expression of gus gene that linked to the antifreeze protein gene was detected, and the results was that the gus gene can express strongly and instantaneously.

关键词： vector construction antifreeze protein control sequence

Cross-species recognition of bat coronavirus RsYN04 and cross-reaction of SARS-CoV-2 antibodies against the virus

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Zoological Research 2023年第6期44卷 1015-1025页

作者： Runchu Zhao Sheng Niu Pu Han Yue Gao Dezhi Liu Chunliang Luo Honghui Liu Bo Liu Yanli Xu Jianxun Qi Zhihai Chen Weifeng Shi lili wu George F.Gao Qihui Wang Institute of Physical Science and Information Anhui UniversityHefeiAnhui 230039China CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China College of Veterinary Medicine Shanxi Agricultural UniversityJinzhongShanxi 030801China School of Life Sciences Hebei UniversityBaodingHebei 071002China Center of Infectious Disease Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases in Universities of Shandong Shandong First Medical University&Shandong Academy of Medical SciencesTaianShandong 271000China University of Chinese Academy of Sciences Beijing 100049China

Following the outbreak of coronavirus disease 2019(COVID-19),several severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)-related coronaviruses have been *** research has identified a novel lineage of SARS-CoV-2-related CoVs in bats,including RsYN04,which recognizes human angiotensin-converting enzyme 2(ACE2)and thus poses a potential threat to ***,we screened the binding of the RsYN04receptor-binding domain(RBD)to ACE2 orthologs from 52animal species and found that the virus showed a narrower ACE2-binding spectrum than ***,the presence of the T484W mutation in the RsYN04 RBD broadened its *** also evaluated 44 SARS-CoV-2antibodies targeting seven epitope communities in the SARS-CoV-2 RBD,together with serum obtained from COVID-19 convalescents and vaccinees,to determine their cross-reaction against *** showed that no antibodies,except for the RBD-6 and RBD-7 classes,bound to the RsYN04 RBD,indicating substantial immune differences from ***,the structure of the RsYN04 RBD in complex with cross-reactive antibody S43 in RBD-7 revealed a potently broad epitope for the development of therapeutics and *** findings suggest RsYN04 and other viruses belonging to the same clade have the potential to infect several species,including humans,highlighting the necessity for viral surveillance and development of broad anticoronavirus countermeasures.

关键词： SARS-CoV-2-related coronavirus RsYN04 ACE2 Antibody Structure

Antiplasticizing Effect of MOCA on Poly(vinyl chloride)

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Journal of wuhan University of Technology(Materials Science) 2011年第1期26卷 83-87页

作者：张藕生张超灿 School of Materials Science and Engineering Wuhan University of Technology

An obvious antiplasticizing effect has been observed in PVC with small amount of MOCA, 3,3’-dichloro-4,4’-diamino-diphenylmethane. PVC-MOCA interaction and crystallization behavior of PVC/MOCA blends were investigated in detail to explain the mechanism of antiplasticization on the basis of a series of techniques including DMA, FTIR, and DSC. The results of mechanical properties tests show that the tensile strength of PVC with 5 phr of MOCA reaches a maximum value, 69.5 Mpa, which is about 23 % higher than that of pure PVC. The rise in tensile strength was attributed to an antiplasticizing effect of MOCA on PVC as confirmed by DMA measurements. The evidences from FTIR reveal that a strong hydrogen-bonding interaction takes place between the nitrogen atom of –NH2 groups in MOCA and the methine proton of PVC repeat units. The results of DSC analysis indicate that crystallization behavior of MOCA is suppressed completely and the crystallinity of PVC decreases with the increase of MOCA amount.

关键词： poly(vinyl chloride) MOCA antiplasticization crystallinity interaction

Cloning and expression analysis of a dirigent protein gene from the resurrection plant Boea hygrometrica

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Progress in Natural Science:Materials International 2009年第3期19卷 347-352页

作者： Renhua wu, lili Wang, Zhi Wang, Haihong Shang, Xia Liu, Yan Zhu, Dongdong Qi, Xin Deng * Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Resurrection plants are tolerant to extreme dehydration and are useful model systems to study genes that play a role in drought tolerance. A gene fragment encoding a dirigent protein that is predicted to function in lignin biosynthesis was identified from leaves of the resurrection plant Boea hygrometrica via cDNA microarray screening. A cDNA, designated BhDIR1, containing the complete predicted open reading frame, was obtained by 50-RACE. BhDIR1 transcripts were found to be accumulated in response to changes in plant dehydration status, exogenously applied phytohormones and signaling molecules, and temperature stresses. BhDIR1 encodes a protein of 199 amino acids, which shows 20-40% similarity to dirigent proteins reported from other plants. BhDIR1 is predicted to contain a cleavable signal peptide at the N-terminal, and its plasma membrane/cell wall localization was confirmed using a GFP fusion protein assay. Consistent with this discovery, the acid-soluble lignin content decreased in dehydrated B. hygrometrica leaves. Taken together, our results indicate a protective role for a dirigent protein in response to drought stress by changing the physical characters of lignin which in turn is predicted to affect the mechanical strength and flexibility of the plant cell wall.

关键词： Resurrection plant Dirigent protein Drought Lignin Cell wall

Ge/Pb/Si(111)生长中Ge原子沿团簇边缘扩散的三维Monte-Carlo模拟

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计算物理 2013年第3期30卷 441-446页

作者：吴黎黎吴锋民浙江师范大学凝聚态物理研究所浙江金华321004

用动态Monte-Carlo方法对Ge在单层表面活性剂Pb覆盖的Si(111)表面上沿团簇边缘扩散进行三维模拟.重点讨论Ge原子是否沿团簇边缘扩散,沿边缘扩散时的最大扩散步数及最近邻原子数对三维生长的影响,并计算薄膜表面粗糙度研究三维生长模式.... 详细信息

用动态Monte-Carlo方法对Ge在单层表面活性剂Pb覆盖的Si(111)表面上沿团簇边缘扩散进行三维模拟.重点讨论Ge原子是否沿团簇边缘扩散,沿边缘扩散时的最大扩散步数及最近邻原子数对三维生长的影响,并计算薄膜表面粗糙度研究三维生长模式.模拟表明Ge沿团簇边缘扩散的行为对薄膜生长模式的影响很大,同时讨论了ES势对三维生长模式的影响.

关键词：沿团簇边缘扩散表面活性剂表面粗糙度 Monte-Carlo模拟

含主动限流控制的MMC-HVDC电网直流短路故障电流解析计算

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电工技术学报 2024年第3期39卷 785-797页

作者：吴丽丽茆美琴施永教育部光伏系统研究工程中心(合肥工业大学) 合肥230009

传统的MMC-HVDC电网直流短路故障电流计算一般采用等效RLC电路方法。但是该方法没有考虑主动限流控制(ACLC)对等效电路RLC参数的影响,因此无法直接用于含ACLC的MMC-HVDC电网的直流短路故障电流的解析计算问题。该文通过将ACLC的控制效... 详细信息

传统的MMC-HVDC电网直流短路故障电流计算一般采用等效RLC电路方法。但是该方法没有考虑主动限流控制(ACLC)对等效电路RLC参数的影响,因此无法直接用于含ACLC的MMC-HVDC电网的直流短路故障电流的解析计算问题。该文通过将ACLC的控制效果等效为虚拟阻抗产生的压降,建立了体现ACLC的控制影响的等效RLC电路,以包含电流变化率限流环节的典型ACLC算法为例,推导了直流双极短路故障条件下ACLC的控制参数与虚拟阻抗的映射关系以及相应的直流故障电流解析表达式。最后,以基于含ACLC的半桥型四端MMC-HVDC电网为例,建立PSCAD/EMTDC仿真模型,对采用ACLC的直流双极短路故障电流解析计算结果进行了验证。结果表明:故障后10ms内直流短路故障电流的PSCAD仿真结果与所提出的含ACLC的等效RLC电路解析计算结果的平均误差在8.29%以内,最大误差在10.99%。因此,使用所提出的方法具有工程实用性。

关键词：模块化多电平换流器主动限流控制虚拟阻抗 RLC电路直流短路故障电流计算

小人物与大潮流——清末民初的政治潮流与徐宝山的选择

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民国档案 2021年第1期 70-76页

作者：吴莉莉南京大学历史学院 225000 扬州工业职业技术学院

在清末民初的历史大潮流中,出身卑微的徐宝山,只能算是一个"小人物"。此人年轻时通过打拼成为青帮盐枭,并被清政府收编为缉私营管带。戊戌变法失败后,他参与自立军起义,支持维新派;辛亥革命时,他投身革命阵营,成为南京临时政府的重要武... 详细信息

在清末民初的历史大潮流中,出身卑微的徐宝山,只能算是一个"小人物"。此人年轻时通过打拼成为青帮盐枭,并被清政府收编为缉私营管带。戊戌变法失败后,他参与自立军起义,支持维新派;辛亥革命时,他投身革命阵营,成为南京临时政府的重要武装力量;最后,他投靠袁世凯,成为资产阶级革命派的死敌。徐宝山政治立场的变化,一是使我们在研究孙中山等领袖人物对历史的作用外,可以比较具体地考察"小人物"对历史大潮的影响;二是使我们清晰地回看历史大潮澎湃而来时,除了宏观的正面冲击力、进步性和建设性外,还可以观察其微观方面的某些误缺性、破坏性和复杂性,观察某些政策对不同的小人物命运的主宰和其诉求的忽略;三是提醒我们如何在社会治理过程中,兼顾无数小人物的利益,使他们成为积极的因素,避免大潮流对小人物利益的损害。总之,每一个小人物身上都折射出了历史大潮流的另一个面相,正是无数个不同的面相丰富了我们对历史大潮流的认知。

关键词：小人物大潮流徐宝山多面相复杂性

MiR-340 Reverses Cisplatin Resistance of Hepatocellular Carcinoma Cell Lines by Targeting Nrf2-ARE Antioxidant Pathway

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MiR-340 Reverses Cisplatin Resistance of Hepatocellular Carc...