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Construction of a bivalent vaccine candidate against HAdV4/HAdV7 based on capsid-display strategy via Red-homologous recombination and counter-selection methodology

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Biosafety and Health 2024年第2期6卷 70-79页

作者： Peng Wang Yunting Shao Xichun Yang Wenning Zhang jianguang Zhou Fang Huang Shuang Liu Jiping Zheng Chengjun wu Shanhu Li Department of Cell Engineering Bejing Institute of BiotechnologyBejing 100850China School of Basic Medical Sciences Faculty of MedicineDalian University of TechnologyDalian 116024China Lab of Microbiological Engineering School of Life and Health SciencesHainan UniversityHaikou 570228China

Human adenoviruses(HAdvs)are major respiratory pathogens.Specifically,human adenovirus type 4(HAdV4)and human adenovirus type 7(HAdV7)are known for causing fever and pneumonia,with docu-mented cases of fatalities among the population.In recent years,HAdV4/HAdv7 has been implicated in caus-ing substantial outbreaks,leading to increased morbidity in multiple countries.Most HAdV4 and HAdV7 infections have been reported in North America,Asia,Europe,Africa,South America,Oceania,and the Middle East.Most fatalities occurred in North America(the United States)and Asia(China and Singapore).Engineered recombinant adenoviruses have played a crucial role as vaccine vectors.In this study,we con-structed a recombinant adenovirus,Ad4ITRmut-Ad7E3,and evaluated it in vitro and in vivo.We observed that the replication rate of Ad4ITRmut-Ad7E3 was lower than that of the RI-67 strain,indicating that the mutation of inverted terminal repeats(ITRs)weakened the replication ability of HAdV4.Immunization of BALB/c mice with the bivalent Ad4ITRmut-Ad7E3 vaccine strain,administered by intraperitoneal injection and oral gavage,resulted in the elicitation of neutralizing antibodies targeting HAdV4 and HAdv7.This finding not only pro-vides a novel method and technique for the efficient construction of a polyvalent recombinant adenovirus vac-cine candidate against HAdV4 and HAdv7 but also against other prevalent adenovirus serotypes such as HAdV3,HAdV11,HAdV14,and HAdv55,from various regions.

关键词： Bivalent adenovirusvaccine Capsid-displaystrategy ITRs modification Hexonepitopereplacement

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Resting-state brain network remodeling after different nerve reconstruction surgeries:a functional magnetic resonance imaging study in brachial plexus injury rats

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Neural Regeneration Research 2025年第5期20卷 1495-1504页

作者： Yunting Xiang Xiangxin Xing Xuyun Hua Yuwen Zhang Xin Xue Jiajia wu Mouxiong Zheng He Wang jianguang Xu School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Department of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence(Fudan University) Ministry of EducationShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Distinct brain remodeling has been found after different nerve reconstruction strategies,including motor representation of the affected limb.However,differences among reconstruction strategies at the brain network level have not been elucidated.This study aimed to explore intranetwork changes related to altered peripheral neural pathways after different nerve reconstruction surgeries,including nerve repair,endto-end nerve transfer,and end-to-side nerve transfer.Sprague–Dawley rats underwent complete left brachial plexus transection and were divided into four equal groups of eight:no nerve repair,grafted nerve repair,phrenic nerve end-to-end transfer,and end-to-side transfer with a graft sutured to the anterior upper trunk.Resting-state brain functional magnetic resonance imaging was obtained 7 months after surgery.The independent component analysis algorithm was utilized to identify group-level network components of interest and extract resting-state functional connectivity values of each voxel within the component.Alterations in intra-network resting-state functional connectivity were compared among the groups.Target muscle reinnervation was assessed by behavioral observation(elbow flexion)and electromyography.The results showed that alterations in the sensorimotor and interoception networks were mostly related to changes in the peripheral neural pathway.Nerve repair was related to enhanced connectivity within the sensorimotor network,while end-to-side nerve transfer might be more beneficial for restoring control over the affected limb by the original motor representation.The thalamic-cortical pathway was enhanced within the interoception network after nerve repair and end-to-end nerve transfer.Brain areas related to cognition and emotion were enhanced after end-to-side nerve transfer.Our study revealed important brain networks related to different nerve reconstructions.These networks may be potential targets for enhancing motor recovery.

关键词： brain functional networks end-to-end nerve transfer end-to-side nerve transfer independent component analysis nerve repair peripheral plexus injury resting-state functional connectivity

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Key technology and application of AB_(2) hydrogen storage alloy in fuel cell hydrogen supply system

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Materials Reports(Energy) 2024年第1期4卷 113-122页

作者： Ming Yao jianguang Yuan Bao Zhang Youhua Yan Shaoxiong Zhou Ying wu School of Energy Power and Machinery Engineering North China Electric Power UniversityNo.2 BeinongluChangping DistrictBeijing 102206China Institute of Energy Power Innovation North China Electric Power UniversityNo.2 BeinongluChangping DistrictBeijing 102206China Jiangsu JITRI Advanced Energy&Materials Research Institute Co. LtdNo.5 ChongyinanluXinbei DistrictChangzhou 213000China

At present,there is limited research on the application of fuel cell power generation system technology using solid hydrogen storage materials,especially in hydrogen-assisted two-wheelers.Considering the disadvantages of low hydrogen storage capacity and poor kinetics of hydrogen storage materials,our primary focus is to achieve smooth hydrogen ab-/desorption over a wide temperature range to meet the requirements of fuel cells and their integrated power generation systems.In this paper,the Ti_(0.9)Zr_(0.1)Mn_(1.45)V_(0.4)Fe_(0.15) hydrogen storage alloy was successfully prepared by arc melting.The maximum hydrogen storage capacity reaches 1.89 wt% at 318 K.The alloy has the capability to absorb 90% of hydrogen storage capacity within 50 s at 7 MPa and release 90% of hydrogen within 220 s.Comsol Multiphysics 6.0 software was used to simulate the hydrogen ab-/desorption processes of the tank.The flow rate of cooling water during hydrogen absorption varied in a gradient of(0.02 t x)m s^(-1)(x=0,0.02,0.04,0.06,0.08,0.1,0.12).Cooling water flow rate is positively correlated with the hydrogen absorption rate but negatively correlated with the cost.When the cooling rate is 0.06 m s^(-1),both simulation and experimentation have shown that the hydrogen storage tank is capable of steady hydrogen desorption for over 6 h at a flow rate of 2 L min^(-1).Based on the above conclusions,we have successfully developed a hydrogen-assisted two-wheeler with a range of 80 km and achieved regional demonstration operations in Changzhou and Shaoguan.This paper highlights the achievements of our team in the technological development of fuel cell power generation systems using solid hydrogen storage materials as hydrogen storage carriers and their application in twowheelers in recent years.

关键词： AB_(2)hydrogen storage alloy Hydrogen storage tanks Simulation Hydrogen-electric coupling system Power-assisted two-wheelers

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3D打印碳纤维混凝土断裂力学性能试验研究

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建筑结构学报 2024年第5期45卷 243-252页

作者：岳健广王健吴瑶张怀奎叶子晗张榕南京工业大学土木工程学院江苏南京211816

为研究打印碳纤维混凝土的断裂力学性能,开展劈裂和三点弯试验,分析了打印方式和纤维含量对其力学性能、断裂破坏机理和微观开裂特性的影响规律,从断面材料完整性角度阐释了其各向异性的成因,建立了打印碳纤维混凝土与浇筑混凝土力学性... 详细信息

为研究打印碳纤维混凝土的断裂力学性能,开展劈裂和三点弯试验,分析了打印方式和纤维含量对其力学性能、断裂破坏机理和微观开裂特性的影响规律,从断面材料完整性角度阐释了其各向异性的成因,建立了打印碳纤维混凝土与浇筑混凝土力学性能指标之间的关系表达式。研究结果表明:打印碳纤维混凝土断裂过程与浇筑混凝土的相似,可分为弹性阶段、裂缝稳定扩展阶段和裂缝不稳定扩展阶段;打印方式会引起混凝土不同方向上断面材料完整性削弱程度的不同,相应地引起力学性能不同程度的降低,这是导致打印混凝土各向异性的本质原因;断面材料完整性削弱程度越大,剪切微裂缝数量比例越高,且随着纤维含量增加,纤维桥接作用的增强效果有所降低;由于纤维分布的随机性,力学性能指标衰减系数与剪切裂缝数量占比衰减系数之间的关系呈非线性。

关键词： 3D打印碳纤维混凝土各向异性断裂静力试验材料完整性微观开裂

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Geological Controls on High Production of Tight Sandstone Gas in Linxing Block, Eastern Ordos Basin, China

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Acta Geologica Sinica(English Edition) 2020年第2期94卷 430-443页

作者： QI Yu JU Yiwen MENG Shangzhi YU Kun LI wuyang JIA Tianrang wu jianguang CHEN Wangang LUO long Key Laboratory of Computational Geodynamics College of Earth and Planetary SciencesUniversity of Chinese Academy of SciencesBeijing 100049China Department of Earth Sciences Durham University.Durham.Dill 3LEUK China United Coalbed Methane Co Ltd Beijing 100011China State Key Laboratory Cultivation Base for Gas Geology and Gas Control Henan Polytechnic UniversityJiaozuoHenan 454000China College of Geosciences China University of PetroleumBeijing 102249China

Tight sandstone gas in the Linxing Block, eastern Ordos Basin, has been successfully exploited. The high performance is mainly a result of the special geological conditions. The key geological controls for high production have been discussed on the basis of seismic data, field observation, sample features, mercury porosimetry, mechanical properties, and basin modeling. Firstly, the coal measures have good gas generation potential, not only because of the existence of coalbeds and organic-rich shales, but also because coal laminae and microbial mats in the shales significantly increase their total organic carbon(TOC) contents. Secondly, except for the uplifted zone of the Zijinshan complex and the eastern fault zone, rare large faults develop in the Carboniferous–Permian sequence, ensuing the sealing capacity of cap rock. Small fractures generally concentrated in the sandstones rather than the mudstones. Thirdly, gas accumulation in the Linxing Block was controlled by the tectonic, burial and thermal histories. Gas accumulation in the Linxing Block started in the Late Triassic, followed by three short pauses of thermal maturation caused by relatively small uplifts;the maximum hydrocarbon generation period is the Early Cetaceous as a combined result of regional and magmatic thermal metamorphisms. Field profiles show abundant fractures in sandstone beds but rare fractures in mudstone beds. Mechanical properties, determined by lithostratigraphy, confine the fractures in the sandstones, increasing the permeability of sandstone reservoirs and retaining the sealing capacity of the mudstone cap rocks. The modern ground stress conditions favor the opening of predominant natural fractures in the NNW-SSE and N-S directions. These conclusions are useful for exploring the potential tight sandstone gas field.

关键词： tight sandstone gas structure fracture basin evolution Ordos Basin

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智能康复工程在中西医脑康复中的应用

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上海中医药杂志 2024年第1期58卷 1-7页

作者：吴佳佳郑谋雄华续赟徐建光中医智能康复教育部工程研究中心上海201203 上海中医药大学附属岳阳中西医结合医院康复医学中心上海200437 上海中医药大学康复医学院上海201203

老龄化及其相关疾病已成为全球性的重大挑战,尤其是神经系统疾病,如脑血管病、脑损伤及神经退行性疾病,已成为老龄化人群主要的健康威胁之一。传统康复方法的局限性促使人工智能和康复工程技术进入康复学科,为脑康复带来新的希望。智能... 详细信息

老龄化及其相关疾病已成为全球性的重大挑战,尤其是神经系统疾病,如脑血管病、脑损伤及神经退行性疾病,已成为老龄化人群主要的健康威胁之一。传统康复方法的局限性促使人工智能和康复工程技术进入康复学科,为脑康复带来新的希望。智能康复工程将中医智慧与现代科技相结合,利用大数据、人工智能和康复工程技术及方法,融合中医药学、神经科学和康复医学,提供更精准的神经调控和个性化康复治疗。通过人工智能和康复工程在中西医脑康复领域的综合协同应用,包括融合工程技术与脑功能康复的创新方法、基于神经环路重建机制的脑康复、中医智能康复等,旨在为脑功能康复的方式创新和技术发展提供有力支持。

关键词：脑康复人工智能康复治疗神经系统疾病脑卒中针灸中医功法中西医结合

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隐形矫治器矫治Ⅱ类错[牙合]畸形对颞下颌关节相关角度、间隙变化值的影响

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实用医学杂志 2024年第8期40卷 1121-1125,1131页

作者：胡露露五味子杨梓徐建光安徽医科大学口腔医学院安徽医科大学附属口腔医院合肥230032

目的探讨隐形矫治器矫治Ⅱ类错[牙合]畸形对颞下颌关节的影响。方法选取Ⅱ类错[牙合]畸形患者70例,采用信封法将患者分为观察组(n=35)和对照组(n=35),观察组给予隐形矫治器矫治,对照组给予自锁托槽矫治器矫治。观察两组颞下颌关节相... 详细信息

目的探讨隐形矫治器矫治Ⅱ类错[牙合]畸形对颞下颌关节的影响。方法选取Ⅱ类错[牙合]畸形患者70例,采用信封法将患者分为观察组(n=35)和对照组(n=35),观察组给予隐形矫治器矫治,对照组给予自锁托槽矫治器矫治。观察两组颞下颌关节相关角度、间隙变化值等。结果观察组矫治前后上中切牙长轴与前颅底平面相交的下内角(U1-SN)变化值、上中切牙长轴与鼻根点-上牙槽座点连线交角(U1-NA)变化值分别为(11.05±1.01)°和(4.75±0.76)°,明显高于对照组(P <0.05);观察组矫治前后上颌中切牙、上颌侧切牙、下颌中切牙、下颌侧切牙变化值分别为(0.51±0.21)、(0.40±0.15)、(0.40±0.13)、(0.20±0.05)mm,明显低于对照组(P <0.05);观察组矫治前后菌斑指数(PLI)、牙龈指数(GI)和探诊深度(PD)变化值分别为(0.44±0.19)分、(0.41±0.13)分、(0.38±0.10)mm,明显低于对照组(P <0.05)。结论隐形矫治器矫治Ⅱ类错[牙合]畸形有较好的应用价值,有利于患者颞下颌关节改善。

关键词：隐形矫治器矫治 Ⅱ类错[牙合]畸形颞下颌关节

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国医大师伍炳彩异病同治验案分析

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江西中医药 2024年第2期55卷 37-39页

作者：熊国军伍建光江西中医药大学研究生院南昌330004 江西中医药大学附属医院南昌330006

异病同治是中医治疗的重要原则,不仅体现了中医临床特色,还彰显了辨证论治的精髓。国医大师伍炳彩临床擅用银翘马勃散合酸枣仁汤,认为其所治病证病机特点为“湿热闭阻上焦,肝郁血虚”。通过分析伍炳彩临床使用该方治疗失眠、心悸、闭经... 详细信息

异病同治是中医治疗的重要原则,不仅体现了中医临床特色,还彰显了辨证论治的精髓。国医大师伍炳彩临床擅用银翘马勃散合酸枣仁汤,认为其所治病证病机特点为“湿热闭阻上焦,肝郁血虚”。通过分析伍炳彩临床使用该方治疗失眠、心悸、闭经等3个病案,体现该方临床辨证要点,体现了中医异病同治的精妙。

关键词：伍炳彩银翘马勃散酸枣仁汤异病同治验案

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国医大师伍炳彩运用逍遥散经验浅析

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江西中医药 2024年第5期55卷 36-38页

作者：邱晨韵伍建光江西中医药大学研究生院南昌330004 江西中医药大学附属医院南昌330006

国医大师伍炳彩认为,现代临床内伤杂病以肝病为多,故尤为重视对逍遥散的应用。其认为逍遥散证病位在肝、脾,属气分、血分同病,但以血分为主;病因主要归结于肝气郁结。伍炳彩临床运用逍遥散主要采用汤剂与丸剂2种剂型,有关逍遥散的运用思... 详细信息

国医大师伍炳彩认为,现代临床内伤杂病以肝病为多,故尤为重视对逍遥散的应用。其认为逍遥散证病位在肝、脾,属气分、血分同病,但以血分为主;病因主要归结于肝气郁结。伍炳彩临床运用逍遥散主要采用汤剂与丸剂2种剂型,有关逍遥散的运用思路:一是基于肝郁脾虚的证治机理,二是结合足厥阴肝经循行部位上出现的疼痛或不适,三是抓住肝主疏泄和肝主藏血的生理功能,四是在中医整体观指导下基于五脏相关理论运用逍遥散。

关键词：伍炳彩逍遥散学术经验

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化学杀雄剂CH1诱导的小麦雄性不育花药转录组学及相关基因表达的分析

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麦类作物学报 2024年第3期44卷 289-298页

作者：吴倩郭皓昱席甜甜李煜杨建光马守才王军卫西北农林科技大学农学院陕西杨凌712100

为了揭示化学杀雄剂CH1诱导雄性不育的作用机理,以小麦品种普冰151为试验材料,利用测序技术对化杀不育系(FHS)和正常可育系(FZC,对照)的花药RNA进行转录组学分析,筛选差异表达基因,并进行GO、KEGG富集分析及实时荧光定量PCR分析。结果表... 详细信息

为了揭示化学杀雄剂CH1诱导雄性不育的作用机理,以小麦品种普冰151为试验材料,利用测序技术对化杀不育系(FHS)和正常可育系(FZC,对照)的花药RNA进行转录组学分析,筛选差异表达基因,并进行GO、KEGG富集分析及实时荧光定量PCR分析。结果表明,FHS和FZC之间筛选出差异表达基因共3 895个,其中上调表达基因1 334个,下调表达基因2 561个,主要富集在碳水化合物代谢、多糖代谢、外部结构组织、细胞壁组织等;经KEGG分析,差异基因主要集中在戊糖和葡萄糖醛酸转化、淀粉和蔗糖代谢利用等通路中。利用实时定量PCR技术对转录组数据中所筛选出的5个表达差异较大的基因进行验证,在花药发育的单核期,处理与对照相比,这5个基因表现出不同的上下调趋势;而在二核期和三核期,这5个基因都表现出明显的下调趋势,推测这5个基因的下调表达有可能与化杀不育系花粉的败育有关。

关键词：化学杀雄剂转录组分析 GO富集 KEGG富集荧光定量PCR

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