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Agricultural Sciences in China 2011年第2期10卷 267-274页

作者： wu fei-long LIN Dai-yan SU De-chun College of Resources and Environmental Sciences China Agricultural UniversityBeijing 100193P.R.China Institute of Agricultural Engineering Fujian Academy of Agricultural SciencesFuzhou 350013P.R.China

Pot experiments were conducted under greenhouse condition to investigate whether Cd and Pb uptake by rice could be reduced when it was rotated with oilseed rape and compost *** results showed that the rice grown after oilseed rape had significantly lower Cd and Pb concentrations in both straw and *** and Pb concentrations in the grains of the rice rotated with oilseed rape decreased by approximately 46-80% and 17-86%,respectively,although the Cd and Pb removal by oilseed rape ranged only from 2.39-3.67 and 0.032-0.13% of the total content in *** amendment also decreased the bioavailability of Cd and Pb in the soil and reduced Cd and Pb uptake by oilseed rapes and *** concentrations of Cd and Pb significantly decreased in the exchangeable and carbonate fractions and Pb concentration decreased in the organic matter and sulfide fractions in the contaminated soil after planting oilseed rapes.

关键词： heavy metal crop rotation compost oilseed rape rice

Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

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Journal of Oceanology and Limnology 2020年第2期38卷 364-377页

作者： ZHANG Chan ZHANG Wei-jia YIN Qunjian LI Xuegong QI Xiaoqing wu long-fei Laboratory of Deep Sea Microbial Cell Biology Institute of Deep-sea Science and EngineeringChinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms CNRS-Marseille/CAS-SanyaChina Aix Marseille Univ CNRSLCBMarseille F-13402France

Trimethylamine N-oxide(TMAO)is one of the most important nutrients for bacteria in the deep-sea environment and is capable of improving pressure tolerance of certain bacterial *** assess the impact of TMAO on marine microorganisms,especially those dwelling in the deep-sea environment,we analyzed the bacterial community structure of deep-sea sediments after incubated under different *** at 50 MPa and 0.1 MPa revealed that TMAO imposed a greater influence on bacterial diversity and community composition at atmospheric pressure condition than that under high hydrostatic pressure(HHP).We found that pressure was the primary factor that determines the bacterial ***,in total,238 bacterial strains were isolated from the enrichments,including 112 strains a ffiliated to 16 genera of 4 phyla from the Yap Trench and 126 strains a ffiliated to 11 genera of 2 phyla from the Mariana *** of HHP reduced both abundance and diversity of isolates,while the presence of TMAO mainly af fected the diversity of isolates *** addition,certain genera were isolated only when TMAO was *** together,we demonstrated that pressure primarily defines the bacterial community and culturable bacterial ***,we showed for the first time that TMAO had distinct influences on bacterial community depending on the pressure *** results enriched the understanding of the significance of TMAO in bacterial adaptation to the deep-sea environment.

关键词： deep-sea bacteria high hydrostatic pressure(HHP) trimethylamine N-oxide(TMAO) community structure

Identification and characterization of a novel elastase inhibitor from Hirudinaria manillensis

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Chinese Journal of Natural Medicines 2021年第7期19卷 540-544页

作者： XU Kuan-Hong ZHOU Meng wu fei-long TANG Xiao-Peng LU Qiu-Min LAI Ren long Cheng-Bo School of Life Sciences University of Science and Technology of ChinaHefei 230027China Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences Key Laboratory of Bioactive Peptides of Yunnan ProvinceKunming Institute of ZoologyKunming 650223China Chongqing Institute of Medicinal Plant Cultivation Nanchuan 408435China University of Chinese Academy of Sciences Beijing 100049China

A large number of protease inhibitors have been found from leeches,which are essential in various physiological and biological *** the curret study,a novel elastase inhibitor was purified and characterized from the leech of Hirudinaria manillensis,which was named *** structure analysis showed that HMEI-A belonged to a new family of ***-A exerted inhibitory effects on elastase and showed potent abilities to inhibit elastase with an inhibition constant(Ki)of 1.69×10^(−8) mol·L^(−1).Further study showed that HMEI-A inhibited the formation of neutrophil extracellular trap(NET).These results suggested that HMEIA from the leech of *** is a novel elastase inhibitor which can suppress NET *** may play a significant role in blood-sucking of leeches and is a potential candidate as an anti-inflammatory agent.

关键词： Elastase inhibitor Hirudinaria manillensis HMEI-A NET formation Anti-inflammation

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

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Journal of Oceanology and Limnology 2019年第1期37卷 210-222页

作者： YIN Qunjian ZHANG Weijia LI Xuegong ZHOU Lihong QI Xiaoqing ZHANG Chan wu long-fei Laboratory of Deep-sea Microbial Cell Biology Institute of Deep-sea Science and Engineering Chinese Academy of Sciences University of Chinese Academy of Sciences International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms CNRS-Marseille/CAS-Beijing-Qingdao-Sanya CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions Institute of Deep-sea Science and Engineering Chinese Academy of Sciences AMU LCB UMR 7283 CNRS-Marseille

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.

关键词： marine bacteria Trimethylamine N-oxide(TMAO) high hydrostatic pressure(HHP) pressure tolerance phenotype

Archaeal community structure in sediments from a seamount in the Mariana Volcanic Arc

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Journal of Oceanology and Limnology 2019年第4期37卷 1197-1210页

作者： ZHANG Wenyan LIU Jia DONG Yi LI Xuegang XU Cong XIAO Tian PAN Hongmiao wu long-fei Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China International Associated Laboratory of Evolution and Development of Magnetotactic Multicellular Organisms (LIA-MagMC) CNRS-CAS Marseille-Beijing-Qingdao-Sanya Aix Marseille Univ CNRS LCB Marseille 31 chemin Joseph Aiguier F-13402 France Center for Ocean Mega-Science Chinese Academy of Sciences Qingdao 266071 China

Seamounts are subsurface mountains in the ocean. Examination of the abundance and distribution of Archaea in seamount ecosystems may provide a better understanding of their ecological functions. Most studies of marine archaeal assemblages in seamount area have focused on hydrothermal vents or ferromanganese crusts. We investigated the archaeal communities from a seamount of the Mariana Volcanic Arc, in the tropical western Pacific Ocean by using high-throughput sequencing. Thaumarchaeota was dominant in the sediments of all sample stations. Community diversity and species richness were greatest at stations near the top of the seamount, and lowest at the deepest station. One sample station on the steep southeast slope that faced the Yap-Mariana trench had a unique composition of Archaea. In summary, depth has an important influence on archaeal community structure, and the geographic properties and sediment characteristics may explain the unique distribution patterns of Archaea in this seamount. This study provides a foundation for future research on Archaea in seamounts.

关键词： seamount archaeal diversity community structure Nitrosopumilales

Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China

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Acta Oceanologica Sinica 2017年第2期36卷 94-104页

作者： DU Haijian ZHANG Rui ZHANG Wenyan XU Cong CHEN Yiran PAN Hongmiao ZHOU Ke wu long-fei XIAO Tian Key Laboratory for Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China College of Resources and Environment Qingdao Agriculture University Qingdao 266109 China Aix Marseille Université CNRS LCB UMR 7257 Institut de Microbiologie de la Méditerranée Marseille F-13402 France University of Chinese Academy of Sciences Beijing 100049 China

Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake（China）, where their abundance reached 103–104 ind./cm3. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy（HRTEM） analysis show that magnetosome crystals were composed of Fe3O4, and their morphology was specific to particular cell morphotypes. By the 16 S r RNA gene sequence analysis, we found fourteen operational taxonomic units（OTUs） which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the *** with known axenic and uncultured marine magnetotactic bacteria, the 16 S r RNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to96.2%（〈97%）. The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake.

关键词： magnetotactic bacteria magnetosome biodiversity Yuehu Lake intertidal sediments

Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea

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Journal of Oceanology and Limnology 2018年第3期36卷 783-794页

作者： TENG Zhaojie ZHANG Wenyan CHEN Yiran PAN Hongmiao XIAO Tian wu long-fei CAS Key Laboratory of Marine Ecology ＆ Environmental Sciences Institute of OceanologyChinese Academy of Sciences Qingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266000China Laboratoire de Chimie Bacterienne UMR7283Institut de Microbiologie de la MediterraneeAix-Marseille UniversiteCNRS MarseilleFrance Laboratoire International Associe de la Bio-Mineralisation et Nano-Structures CNRSMarseilleFrance

Magnetotactic bacteria are a group of Gram-negative bacteria that synthesize magnetic crystals, enabling them to navigate in relation to magnetic field lines. Morphologies of magnetotactic bacteria include spirillum, coccoid, rod, vibrio, and multicellular morphotypes. The coccid shape is generally the most abundant morphotype among magnetotactic bacteria. Here we describe a species of giant rod-shaped magnetotactic bacteria(designated QR-1) collected from sediment in the low tide zone of Huiquan Bay(Yellow Sea, China). This morphotype accounted for 90% of the magnetotactic bacteria collected, and the only taxonomic group which was detected in the sampling site. Microscopy analysis revealed that QR-1 cells averaged(6.71±1.03)×(1.54±0.20) m m in size, and contained in each cell 42–146 magnetosomes that are arranged in a bundle formed one to four chains along the long axis of the cell. The QR-1 cells displayed axial magnetotaxis with an average velocity of 70±28 mm/s. Transmission electron microscopy based analysis showed that QR-1 cells had two tufts of fl agella at each end. Phylogenetic analysis of the 16 S r RNA genes revealed that QR-1 together with three other rod-shaped uncultivated magnetotactic bacteria are clustered into a deep branch of A lphaproteobacteria.

关键词： Alphaproteobacteria flagella motility rod-shaped magnetotactic bacteria

Crystallization Modulation and Holistic Passivation Enables Efficient Two‑Terminal Perovskite/CuIn(Ga)Se_(2)Tandem Solar Cells

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Nano-Micro Letters 2025年第1期17卷 205-215页

作者： Cong Geng Kuanxiang Zhang Changhua Wang Chung Hsien wu Jiwen Jiang fei long Liyuan Han Qifeng Han Yi‑Bing Cheng Yong Peng State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070People’s Republic of China Triumph Photovoltaic Materials Co. Ltd.No.1001 Yannan AvenueHigh‑Tech Development DistrictBengbuAnhui 233000People’s Republic of China College of Materials Science and Engineering Guilin University of TechnologyGuilin 541004People’s Republic of China State Key Laboratory of Metal Matrix Composites Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Two-terminal(2-T)perovskite(PVK)/CuIn(Ga)Se_(2)(CIGS)tandem solar cells(TSCs)have been considered as an ideal tandem cell because of their best bandgap matching regarding to Shockley–Queisser(S–Q)***,the nature of the irregular rough morphology of commercial CIGS prevents people from improving tandem device *** this paper,D-homoserine lactone hydrochloride is proven to improve coverage of PVK materials on irregular rough CIGS surfaces and also passivate bulk defects by modulating the growth of PVK *** addition,the minority carriers near the PVK/C60 interface and the incompletely passivated trap states caused interface recombination.A surface reconstruction with 2-thiopheneethylammonium iodide and N,N-dimethylformamide assisted passivates the defect sites located at the surface and grain ***,LiF is used to create this field effect,repelling hole carriers away from the PVK and C60 interface and thus reducing *** a result,a 2-T PVK/CIGS tandem yielded a power conversion efficiency of 24.6%(0.16 cm^(2)),one of the highest results for 2-T PVK/CIGS TSCs to our *** validation underscores the potential of our methodology in achieving superior performance in PVK/CIGS tandem solar cells.

关键词： PVK/CIGS TSCs Irregular rough surfaces Modulating the growth PVK/C60 interface recombination

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微波通信技术在输变电工程临时通道的应用

中国电力 2013年第11期46卷 146-149页

作者：吴飞龙福建省电力有限公司福州电业局福建福州350009

为了解决输变电工程建设中OPGW光纤接入新建变电站时间上无法满足二次设备联调需求的问题,针对各种临时方案的局限性,提出结合实际情况采用微波接力通信方式。通过一跳SDH微波设备与现有OPGW光纤组成混合通信链路,临时开通联调通道,满... 详细信息

为了解决输变电工程建设中OPGW光纤接入新建变电站时间上无法满足二次设备联调需求的问题,针对各种临时方案的局限性,提出结合实际情况采用微波接力通信方式。通过一跳SDH微波设备与现有OPGW光纤组成混合通信链路,临时开通联调通道,满足二次继电保护、自动化装置联调时间上的要求。在多项实际工程的应用表明,微波接力通信方式可有效解决光纤进站和联调工作时间节点上的矛盾,并可结合实际情况扩大应用范围,推广应用前景广阔。

关键词：微波接力 OPGW SDH STM-1光口输变电工程

Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

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Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： fei Lv longjie Liu Jiazhe wu Pengfei Wang Lixia Pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy