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Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice

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Chinese Science Bulletin 2012年第4期57卷 336-343页

作者： YE ShuiFeng YU Shunwu SHU LieBo wu JinHong wu aizhong LUO LiJun Shanghai Agrobiological Gene Center Shanghai 201106China School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 201101China Shanghai Academy of Agricultural Sciences Shanghai 201106China

Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phyto-hormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72.90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93.04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.

关键词：表达谱分析热休克蛋白蛋白基因水稻品种生命周期逆境蛋白质折叠蛋白质组分

Subcellular localization of the stripe disease-specific protein encoded by rice stripe virus (RSV) in its vector,the small brown planthopper,Laodel-phax striatellus

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Chinese Science Bulletin 2001年第21期46卷 1819-1822页

作者： wu aizhong ZHAO Yan QU Zhicai SHEN Daleng PAN Chongguang SU Deming School of Life Sciences Fudan UniversityShanghai 200433China College of Agronomy Shanghai Jiaotong UniversityShanghai 201101China

The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal of the virus in its vector, the small brown lanthopper, Laodelphax striatellus Fallen. Immunogold particles in large numbers were detected in various parts of the viruliferous females: the ovum, surface of chorion, the mid-gut lumen, and the columnar cells. Whereas there was noneof these particles in the non-viruliferous females and males, and testis of viruliferous males. Endosymbionts (mycetocytes) were abundant, harboring ovaries of both viruliferous and non-viruliferous females, but none in the testis of males. The results provided us with the direct proof that RSV is a ciruculative and propagative plant virus and it was transovarially transmitted alongside with endosymbionts of its vector. Therefore, we deem it a nice lead for future studies on the mechanism of RSV transmission and functioning of its viral proteins.

关键词： Laodelphax striatellus rice stripe virus midgut ovary immunogold labeling immunoelectron microscopy.

Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

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Progress in Natural Science:Materials International 2005年第5期15卷 407-413页

作者： PAN Junsong*, WANG Gang*, LI Xiaozun, HE Huanle, wu aizhong and CAI Run**(Agriculture and Biology School, Shanghai Jiaotong University, Shanghai 201101, China) Agric. and Biology Sch. Shanghai Jiaotong Univ. Shanghai 201101 China

With an F 2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F 2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5, and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group Ⅸ, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively.

关键词： cucumber (Cucumis sativus L.) SRAP (sequence-related amplified polymorphism) linkage map first flower node.

地铁车辆弓网静态电接触的多场耦合模型及温升分析

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机车电传动 2023年第1期 138-143页

作者：闭永雷吴爱中翁琳上海工程技术大学城市轨道交通学院上海201620 上海市轨道交通振动与噪声控制技术工程研究中心上海201620

当地铁到站或在线路上因故临时停车时,接触网仍需为列车提供电流,此时弓网接触部件因处于静止状态而存在局部过热的情况,严重时可能导致滑板热损伤甚至造成接触线断线事故。因此,地铁停车期间弓网电接触的温升研究具有重要意义。地铁列... 详细信息

当地铁到站或在线路上因故临时停车时,接触网仍需为列车提供电流,此时弓网接触部件因处于静止状态而存在局部过热的情况,严重时可能导致滑板热损伤甚至造成接触线断线事故。因此,地铁停车期间弓网电接触的温升研究具有重要意义。地铁列车弓网接触是热、力、电多物理场耦合的复杂行为,而弓网接触力、滑板热导率和接触线截面形状对弓网电接触有重要影响。本文针对受电弓滑板与接触线静态取流的工况,研究了接触电阻和接触热导的变化规律与建模方法,建立了热-力-电耦合的三维有限元分析模型,分析弓网接触力、滑板材料热传导率以及接触线磨耗对接触温升的影响。结果显示,增大弓网接触力、选用高热导率的滑板材料和改良接触线截面轮廓均可降低弓网接触温升。研究结果对接触线结构优化、滑板材料的选取及保障列车安全运行有重要参考价值。

关键词：地铁车辆弓网系统电接触多场耦合温升分析接触电阻

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球-平板微动接触的安定和磨损行为研究

机械工程学报 2015年第5期51卷 105-113页

作者：吴爱中钱洪符栋良上海市特种设备监督检验技术研究院上海200333 上海交通大学安泰经济与管理学院上海200052

针对弹塑性球形接触由黏结变微滑进而发生整体滑动的摩擦力学过程,引入一种基于有限元的黏滑分析方法。在此基础上,建立了弹塑性半球与刚性平板微动接触的有限元模型,特别是在输入模型参数中嵌入了材料延性断裂的判据。不考虑材料断裂... 详细信息

针对弹塑性球形接触由黏结变微滑进而发生整体滑动的摩擦力学过程,引入一种基于有限元的黏滑分析方法。在此基础上,建立了弹塑性半球与刚性平板微动接触的有限元模型,特别是在输入模型参数中嵌入了材料延性断裂的判据。不考虑材料断裂的分析结果表明,切向力控制的周期重复加载导致接触体弹性安定,而位移控制的重复加载引起塑性安定。采用单元删除法研究了接触面材料因塑性累积造成的疲劳断裂,模拟了微动磨损等复杂接触现象。

关键词：球形接触局部微滑安定微动

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超声振动输送的物料纵向振动过程测试与分析

机械科学与技术 2009年第9期28卷 1130-1134页

作者：何勍吴爱中辽宁工业大学振动工程研究所锦州121001

针对超声物料输送中的轻微动态周期接触过程,采用电接触法与激光测振法相结合的测试技术测定超声微幅振动环境下振子与物料试件之间的间歇接触状态。考察了轮廓接触面积及接触界面法向接触刚度对动态接触过程的影响,最后讨论了振子振动... 详细信息

针对超声物料输送中的轻微动态周期接触过程,采用电接触法与激光测振法相结合的测试技术测定超声微幅振动环境下振子与物料试件之间的间歇接触状态。考察了轮廓接触面积及接触界面法向接触刚度对动态接触过程的影响,最后讨论了振子振动特性对动态接触过程的影响。实验结果表明:接触界面上微凸体的纵向刚度差异是决定高频振子与试件接触与脱离的关键因素。

关键词：超声输送超声振动接触刚度压电振子

Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits

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Science China(Life Sciences) 2005年第3期48卷 213-220页

作者： WANG Gang PAN Junsong LI Xiaozun HE Huanle wu aizhong CAI Run Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101 China Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101 China Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101 China Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101 China Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101 China Agriculture and Biology School of Shanghai Jiaotong University Shanghai201101 China

Using SRAP (sequence-related amplified polymorphism) markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F2 individuals derived from a cross of the two cucumber lines, S06 and S52. In the survey of parental polymorphisms with 182 primer combinations, 64 polymorphism-revealing primer pairs were screened out, which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair. The constructed molecular linkage map included 92 loci, distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci. Based on this linkage map, the quantitative trait loci (QTL) for the lateral branch number (lbn) and the lateral branch average length (lbl) in cucumber were identified by QTLMapper1.6. A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63% of the total variation with its positively effecting allele from S06. A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38% of the total variation with its positively effecting allele from S06.

关键词： cucumber, SRAP (sequence-related amplified polymorphism), molecular linkage map, lateral branch gene, quantitative trait loci (QTL).

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电梯悬挂钢丝绳安全系数的辨析

现代制造工程 2019年第8期 135-140页

作者：吴爱中符栋良上海工程技术大学城市轨道交通学院上海201620 上海商学院管理学院上海201400

电梯悬挂钢丝绳是极为关键的承载部件,除了满足确定载荷下的力学强度以外,还应具有足够的安全余量,以便应对其他不确定的载荷情况。国内外电梯技术标准都对悬挂钢丝绳的安全系数提出了要求,但不同标准对安全系数的考量要求各不相同。为... 详细信息

电梯悬挂钢丝绳是极为关键的承载部件,除了满足确定载荷下的力学强度以外,还应具有足够的安全余量,以便应对其他不确定的载荷情况。国内外电梯技术标准都对悬挂钢丝绳的安全系数提出了要求,但不同标准对安全系数的考量要求各不相同。为了辨识这些差别,首先对电梯悬挂钢丝绳典型失效模式进行总结和分析;然后比较中国标准、欧洲标准和美国ASME标准对电梯悬挂钢丝绳安全系数的具体要求,讨论悬挂钢丝绳安全系数的计算方法;最后对电梯悬挂钢丝绳安全系数的取值范围进行了分析和讨论。研究结果有助于理解电梯悬挂钢丝绳安全系数的力学意义,对电梯悬挂钢丝绳的安全评价具有一定的参考作用。

关键词：安全系数悬挂钢丝绳曳引电梯断绳

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基于BESO算法的城市轨道车轮拓扑优化

机械工程学报 2020年第10期56卷 191-199页

作者：文永蓬郑晓明吴爱中刘跃杰上海工程技术大学城市轨道交通学院上海201620

城市轨道车轮的结构优化大多集中于参数优化,设计空间普遍有一定的局限性。以降低城市轨道车轮辐板处的结构应力为目标,利用双向渐进结构优化算法对直辐板车轮进行拓扑优化建模,提出一种新颖的城市轨道车轮辐板结构。重点针对城市轨道... 详细信息

城市轨道车轮的结构优化大多集中于参数优化,设计空间普遍有一定的局限性。以降低城市轨道车轮辐板处的结构应力为目标,利用双向渐进结构优化算法对直辐板车轮进行拓扑优化建模,提出一种新颖的城市轨道车轮辐板结构。重点针对城市轨道车轮受力最恶劣的曲线工况,对直辐板车轮的设计域进行2D拓扑设计,在满足体积约束条件下,通过对删除率、进化率、增加率进行设定,使体积比经过反复振荡从而达到较好的性能优化指标,提高了城市轨道车轮的应力性能,获得了城市轨道车轮的优秀拓扑结构。通过与成熟有限元软件计算结果对比,优化后的静强度和疲劳强度均满足要求,证明了双向渐进结构优化算法的有效性和可靠性。研究结果表明,双向渐进结构优化算法对城市轨道车轮的优化设计是适用的;优化车轮的辐板形式由直辐板进化为曲辐板,且单元向中间圆弧处靠拢,辐板具有不等厚特征;在不增加车轮重量的前提下,优化车轮在辐板处的最大结构应力降低了17%。所做工作对城市轨道车轮结构应力性能的提升具有重要的参考价值。

关键词：城市轨道车轮拓扑优化 BESO算法结构应力直辐板车轮