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Science China Chemistry 2009年第8期52卷 1258-1263页

作者： DU ZhengQing wu wenjie ZHOU YuFen Department of Environmental Science Shanxi University of Finance and EconomicsTaiyuan 030006China

The effects of rises in external K+(Kext) were examined on the hyperpolarization-activated cation current(Ih) in rat dorsal root ganglion neurons using the whole-cell patch clamp *** results showed that Kext increased Ih in a certain concentration and voltage-dependent *** the basal Kext level(4 mmol/L),Ih had a maximal amplitude of 1085 ± 340 pA which was enhanced by ～45% and ～92% at 8 and 16 mmol/L Kext,*** midpoint activation voltage was significantly shifted from -98 mV in the hyperpolarizing direction by 8 and 12 mV at 8 and 16 mmol/L Kext,respectively with alteration of the activation course of *** short time constants of activation became longer with the increasing amplitude of the command potential upon rises in *** long time constants became *** reversal potentials were shifted in the positive direction without significant alterations upon rises in *** to the functional role of Ih,Kext increased Ih,resulting in an enhanced neuronal excitability,which might produce activation potential abnormality and perhaps neuropathic pain involved.

关键词： hyperpolarization-activated inward current external potassium dorsal root ganglion neurons patch clamp

Dynamic response of the cell traction force to osmotic shock

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Microsystems & Nanoengineering 2023年第5期9卷 321-331页

作者： Yongman Liu wenjie wu Shuo Feng Ye Chen Xiaoping wu Qingchuan Zhang Shangquan wu School of Biomedical Engineering Anhui Medical University230032 HefeiChina CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsCAS Center for Excellence in Complex System MechanicsUniversity of Science and Technology of China230026 HefeiChina

Osmotic pressure is vital to many physiological activities,such as cell proliferation,wound healing and disease ***,how cells interact with the extracellular matrix(ECM)when subjected to osmotic shock remains ***,we visualize the mechanical interactions between cells and the ECM during osmotic shock by quantifying the dynamic evolution of the cell traction *** show that both hypertonic and hypotonic shocks induce continuous and large changes in cell traction ***,the traction force varies with cell volume:the traction force increases as cells shrink and decreases as cells ***,the direction of the traction force is independent of cell volume changes and is always toward the center of the cell-substrate ***,we reveal a mechanical mechanism in which the change in cortical tension caused by osmotic shock leads to the variation in traction force,which suggests a simple method for measuring changes in cell cortical *** findings provide new insights into the mechanical force response of cells to the external environment and may provide a deeper understanding of how the ECM regulates cell structure and function.

关键词： traction healing wound

Spatio-temporal Analysis of Urban Spatial Interaction in Globalizing China—A Case Study of Beijing-Shanghai Corridor

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Chinese Geographical Science 2009年第2期19卷 126-134页

作者： wu wenjie ZHANG Wenzhong JIN Fengjun DENG Yu Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

This paper aims to explore urban geography with a new perspective. Endowed with the urban geography connotations, an improved data field model is employed to integrate temporal dimension into spatial process of cities in a typical region in this article. Taking the Beijing-Shanghai Corridor including 18 cities as an example, the authors chose the city centricity index (CCI) and the spatial data field model to analyze the evolution process and features of sub-region and urban spatial interaction in this corridor based on the data of 1991, 1996 and 2002. Through the analy- sis, we found that: 1) with the improvement of the urbanization level and the development of urban economy, the cit- ies’ CCI grew, the urban spatial radiative potential enhanced and the radiative range expanded gradually, which reflects the urban spatial interaction’s intensity has been increasing greatly; 2) although the spatial interaction intensity among the cities and sub-regions in the Beijing-Shanghai Corridor was growing constantly, the gap of the spatial interaction strength among different cities and sub-regions was widening, and the spatial division between the developed areas and the less developed areas was obvious; and 3) the intensity of the spatial interaction of Beijing, Shanghai and their urban agglomerations was far greater than that in small cities of other parts of the corridor, and it may have a strong drive force on the choice of spatial location of the economic activities.

关键词： urban spatial interaction data field Beijing-Shanghai Corridor China

Nanomechanical sensor for rapid and ultrasensitive detection of tumor markers in serum using nanobody

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Nano Research 2022年第2期15卷 1003-1012页

作者： Depeng Rao Kainan Mei Tianhao Yan Yu Wang wenjie wu Ye Chen Jianye Wang Qingchuan Zhang Shangquan wu CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Reproductive Medicine Center Department of Obstetrics and GynecologyFirst Affiliated Hospital of Anhui Medical UniversityHefei 230022China

Early cancer diagnosis requires ultrasensitive detection of tumor markers in *** this end,we develop a novel microcantilever immunosensor using nanobodies(Nbs)as *** the smallest antibody(Ab)entity comprising an intact antigen-binding site,Nbs achieve dense receptor layers and short distances between antigen-binding regions and sensor surfaces,which significantly elevate the generation and transmission of surface *** to the inherent thiol group at the C-terminus,Nbs are covalently immobilized on microcantilever surfaces in directed orientation via one-step reaction,which further enhances the stress *** microcantilever-based nanomechanical sensor,these advantages dramatically increase the sensor ***,Nb-functionalized microcantilevers can detect picomolar concentrations of tumor markers with three orders of magnitude higher sensitivity,when compared with conventional Ab-functionalized *** proof-of-concept study demonstrates an ultrasensitive,label-free,rapid,and low-cost method for tumor marker ***,interestingly,we find Nb inactivation on sensor interfaces when using macromolecule blocking *** adsorption-induced inactivation is presumably caused by the change of interfacial properties,due to binding site occlusion upon complex coimmobilization *** findings are generalized to any coimmobilization methodology for Nbs and,thus,for the construction of high-performance immuno-surfaces.

关键词： nanobody-based biosensor stress enhancement early cancer detection tumor markers adsorption-induced inactivation

Texture adjustment approach of magnesium alloys via variable strain path calculated by an integrated finite element-viscoplastic self-consistent model

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Journal of Materials Science & Technology 2024年第17期184卷 15-31页

作者： wenjie wu Wenzhen Chen Xiaoyu Wang Wenke Wang Wencong Zhang Xinhua Liu Hyoung Seop Kim School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China Key Laboratory for Advanced Materials Processing(MOE) Institute for Advanced Materials and TechnologyUniversity of Science and Technology BeijingBeijing 100083China Graduate Institute of Ferrous&Energy Materials Technology(GIFT) Pohang University of Science and Technology(POSTECH)Pohang 37673South Korea Advanced Institute for Materials Research(WPI-AIMR) Tohoku UniversitySendai 9808577Japan

An integrated calculated approach based on weakly coupled finite element(FEM)-viscoplastic selfconsistent(VPSC)model was established to simulate the texture evolution during the variable strain path extrusion process of magnesium *** spiral die extrusion(SDE)process with additional circumferential shear deformation was applied to investigate the effect of path control on texture adjustment and verify the accuracy of the *** results indicated that the additional spiral shear resulting from the overall inclined flow path effectively reduced the intensity of the{0002}//ED fiber texture by suppressing basal slip activation in the core area,while the local shear deformation along the spiral equal channel strain path led to the formation of an inclined{0002}//ND plane texture on the *** the modified Hall-Petch relationship,the correlation between texture and yield strength was ***,the weakening of the texture effectively suppressed{10-12}tensile twinning,which compensated for the deficiency of compressive yield strength without significantly sacrificing tensile yield strength,and thus improved the tension-compression ***,the strongly inclined{0002}//ND plane texture inhibited the widespread activation of basal slip during tensile yielding,thereby enhancing the yield strength.

关键词： Integrated calculated approach Strain path control Texture adjustment Spiral die extrusion Viscoplastic self-consistent

Nanomechanical assay for ultrasensitive and rapid detection of SARS-CoV-2 based on peptide nucleic acid

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Nano Research 2023年第1期16卷 1183-1195页

作者： Yu Wang Tianhao Yan Kainan Mei Depeng Rao wenjie wu Ye Chen Yongpei Peng Jianye Wang Shangquan wu Qingchuan Zhang CAS Key Laboratory of Mechanical Behavior and Design of Material Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Reproductive Medicine Center Department of Obstetrics and GynecologyFirst Affiliated Hospital of Anhui Medical UniversityHefei 230022China

The massive global spread of the COVID-19 pandemic makes the development of more effective and easily popularized assays ***,we developed an ultrasensitive nanomechanical method based on microcantilever array and peptide nucleic acid(PNA)for the detection of severe acute respiratory syndrome-coronavirus-2(SARS-CoV-2)*** method has an extremely low detection limit of 0.1 fM(105 copies/mL)for N-gene specific sequence(20 bp).Interestingly,it was further found that the detection limit of N gene(pharyngeal swab sample)was even lower,reaching 50 copies/*** large size of the N gene dramatically enhances the sensitivity of the nanomechanical sensor by up to three orders of *** detection limit of this amplification-free assay method is an order of magnitude lower than RT-PCR(500 copies/mL)that requires *** non-specific signal in the assay is eliminated by the in-situ comparison of the array,reducing the false-positive misdiagnosis *** method is amplification-free and label-free,allowing for accurate diagnosis within 1 *** strong specificity and ultrasensitivity allow single base mutations in viruses to be distinguished even at very low ***,the method remains sensitive to fM magnitude lung cancer marker(miRNA-155).Therefore,this ultrasensitive,amplification-free and inexpensive assay is expected to be used for the early diagnosis of COVID-19 patients and to be extended as a broad detection tool.

关键词： SARS-CoV-2 nucleic acid assay nanomechanical sensoring peptide nucleic acid amplification-free

Enhanced orange emission by doping CeB_6 in CaAlSiN_3:Ce^(3+) phosphor for application in white LEDs

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Journal of Rare Earths 2018年第12期36卷 1250-1255页

作者： wenjie wu Kefu Chao Wenquan Liu Lesi Wei Dahai Hu Taiyang Wang O. Tegus College of Physics and Electronic Information Inner Mongolia Normal University Inner Mongolia Key Laboratory for Physics and Chemistry of Functional Materials Leshan Vocational and Technical College

A series of CeB6-doped and CeO2-doped Ca1-xSiAlN3:xCe3+(denoted as CASN:Ce3+@CeB6 and CASN:Ce3+@CeO2, respectively) were synthesized by alloy-nitridation method under high-purity nitrogen atmosphere. The morphologies, crystal phases, and luminescence properties were investigated in *** an increase in the concentration of CeB6, the unit cell volume of CASN:Ce3+@CeB6slightly increases due to the substitution between ions, which leads to a change of micro structure around Ce3+. CASN:Ce3+@CeB6 efficiently emits yellow-orange light with a maximum emission intensity at around 550 nm for the content x of 0.01(being in comparable situation, CASN:Ce3+@CeO2is x = 0.04) when excited at460 nm. Compared with CASN:Ce3+@CeO2, the red emission component of Ce3+ in CASN:Ce3+@CeB6 is much stronger. This is ascribed to energy transfer of intra-Ce3+(within one Ce3+ ion) and inter-Ce3+(between Ce3+ and Ce3+ ions). In addition, the replacements of N3-(0.132 nm for CN = 4) and O2-(0.124 nm for CN = 4) by B2-(0.140 nm for CN = 4), which can lead to a marked expansion of the host lattice and a decrease of the oxidation of samples, are also responsible for the increase of red emission component. Furthermore, CASN:Ce3+@CeB6phosphor has an excellent thermal stability because of the partial substitution of Ce-O(Ce-N) bonds by more covalent Ce-B. As a result, the outstanding luminescent properties of CASN:Ce3+@CeB6 phosphor make it practical to use in the single phosphor-coated high-color-rendering power white LED.

关键词： White LED Nitride CaAlSiN_3:Ce^(3+) CeB_6 Rare earths

Clean production of Fe-based amorphous soft magnetic alloys via smelting reduction of high-phosphorus iron ore and apatite

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International Journal of Minerals,Metallurgy and Materials 2023年第12期30卷 2356-2363页

作者： Hua Zhang Tuoxiao Wang Guoyang Zhang wenjie wu Long Zhao Tao Liu Shuai Mo Hongwei Ni The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081China College of Nuclear Equipment and Nuclear Engineering Yantai UniversityYantai 264005China

Separated preparation of prealloys and amorphous alloys results in severe solidification-remelting and beneficial element removal-readdition contradictions,which markedly increase energy consumption and *** study offered a novel strategy for the direct production of FePC amorphous soft magnetic alloys via smelting reduction of high-phosphorus iron ore(HPIO)and ***,the thermodynamic conditions and equilibrium states of the carbothermal reduction reactions in HPIO were calculated,and the element content in reduced alloys was theoretically *** phase and structural evolutions,as well as element migration and enrichment behaviors during the smelting reduction of HPIO and Ca_(3)(PO_(4))_(2),were then experimentally *** addition of Ca_(3)(PO_(4))_(2)in HPIO contributes to the enrichment of the P element in reduced alloys and the subsequent development of Fe_(3)P and Fe_(2)P *** content of P and C elements in the range of 1.52 wt% -14.63 wt% and 0.62 wt% -2.47 wt%,respectively,can be well tailored by adding 0-50 g Ca_(3)(PO_(4))_(2)and controlling the C/O mole ratio of 0.8-1.1,which is highly consistent with the calculated *** FePC alloys were then successfully formed into amorphous ribbons and *** energy consumption of the proposed strategy was estimated to be 2.00×10^(8) kJ/t,which is reduced by 30% when compared with the conventional production *** results are critical for the comprehensive utilization of mineral resources and pave the way for the clean production of Fe-based amorphous soft magnetic alloys.

关键词： high-phosphorus iron ore smelting reduction structural evolution Fe-based amorphous alloy clean production

Photodegradation-Induced Turn-On Luminescence of Tetraphenylethylene-Based Trithiocarbonate Polymers

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Chinese Journal of Chemistry 2021年第10期39卷 2837-2842页

作者： Hanchen Shen wenjie wu Shunjie Liu Jing Zhang Jacky WYLam Ben Zhong Tang Department of Chemistry Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction and Institute of Advanced StudyThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Department of Laboratory Medicine Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515China HKUST Shenzhen Research Institute No.9 Yuexing 1 st RDSouth Area Hi-tech ParkNanshanShenzhenGuangdong518057 China Center for Aggregation-induced Emission SCUT-HKUST Joint Research InstituteState Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhouGuangdong510640 China HKUST AIE Institute Guangzhou Development DistrictHuangpuGuangzhouGuangdong510530 China Guangdong-Hong Kong-Macao Joint laboratory of Optoelectronic and Magnetic Functional Materials The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina

Main observation and conclusion Fluorescent intelligent materials have attracted wide attention because of their great potential *** major hurdle for the development and application of fluorescent intelligent materials is the aggregation-caused quenching effect in the solid ***,tetraphenylethylene-based trithiocarbonate polymers with satisfactory molecular weights(Mw up to 24900)were synthesized through a one-pot polymerization route under mild *** polymers were non-emissive due to the quenching effect of the trithiocarbonate ***,upon UV irradiation,the polymers degraded and strong emission from the tetraphenylethylene unit was *** a unique property endows them with great potential applications,such as photopatterning,anti-counterfeit labels,and UV detection.

关键词： Photochemistry Trithiocarbonate polymers Luminescence Polymerization Aggregation-induced emission

Study on the adaptability of traditional architecture in agricultural heritage sites after tourism intervention—a case study of Huzhou Digang Food Street in China

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Built Heritage 2022年第3期6卷 52-64页

作者： Ying Wang Yehong Sun Xingguo Gu wenjie wu Cancan Yao Tourism College of Zhejiang Hangzhou 311231People’s Republic of China. Tourism College of Beijing Union UniversityBeijing 100101People’s Republic of China. Institute of Rural Development Zhejiang Academy of Agricultural SciencesHangzhou 310021People’s Republic of China. Key Laboratory of Creative Agriculture Ministry of Agriculture and Rural Afairs of ChinaHangzhou 310021People’s Republic of China.

Traditional architecture in agricultural heritage sites(AHSs)embodies the livelihood of local communities and residents,which is an important part of the ***,with the intervention of tourism,some of the traditional architecture in AHSs has gradually transformed into simple use for tourism,without refecting the cultural connotations of agricultural heritage systems,as well as the farming wisdom of the *** paper takes Digang Food Street in Huzhou,China as an example,which is in the core protection area of the Huzhou mulberry-dyke and fsh-pond system,combining comparative analysis and geographical system analysis to explore the adaptive transformation in decoration,structure,spatial layout and functional form of buildings after the development of the tourism *** dynamic relationship among traditional architecture,sustainable development of tourism and conservation of agriculture heritage is also analysed,expecting to provide some experience and reference for the conservation of other AHSs.

关键词： Traditional architecture Tourism intervention Agricultural heritage systems Adaptability