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Hydrothermal c.rbonization of livestoc. mortality for the reduc.ion of pathogens and mic.obially-derived DNA

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Frontiers of Environmental Sc.enc. & Engineering 2017年第3期11卷 107-114页

作者： Thomas F. Duc.y Jessic. c. c.llins Kyoung S. Ro Bryan L. Woodbury D. Dee Griffin c.astal Plains Soil Water and Plant Research Center Agricultural Research Service United States Department of Agriculture Florence SC 29501 USA South c.rolina Governor's Sc.ool for Sc.enc. and Mathematic. Hartsville SC 29550 USA U.S. Meat Animal Researc. c.nter Agricultural Research Service United States Department of Agriculture Clay Center NE 68933 USA University of nebraska-Linc.ln Great Plains Veterinary Education Center Lincoln NE 68588 USA

Hydrothermal c.rbonization （HTc., utilizing high temperature and pressure, has the potential to treat agric.ltural waste via inac.ivating pathogens, antibiotic.resistanc. genes （ARG）, and c.ntaminants of emerging c.nc.rn （c.c. in a environmental and ec.nomic.l manner. Livestoc. mortality is one fac.t of agric.ltural waste that c.n pose a threat to the surrounding environment. While several methods are utilized to treat livestoc. mortality, there remains a pauc.ty of data on the elimination of mic.obially- derived DNA in these treatment prac.ic.s. This DNA, most notably ARGs, if it survives treatment c.n be reintroduc.d in agric.ltural environments where it c.uld potentially be passed to pathogens, posing a risk to animal and human populations. HTc.treatments have been suc.essfully utilized for the treatment of c.c., however very little is understood on how ARGs survive HTc.treatment. This study aims to fill this knowledge gap by examining the survivability ofmic.obially-derived DNA in the HTc.treatment of livestoc. mortality. We examined three treatment temperatures （100℃, 150℃, and 200℃） at autogenic.pressures at three treatment times （30, 60, and 240 min）. We examined the amplific.tion of a plasmid-bome reporter gene c.rried by Esc.eric.ia c.li DH 10B introduc.d to both beef bone and tissue. Results indic.te that while all three temperatures, at all treatment times, were suitable for c.mplete pathogen kill, only temperatures of 150℃ and 200℃ were suffic.ent for eliminating mic.obial DNA. These results serve as the basis for future potential HTc.treatment rec.mmendations for livestoc. mortality when c.nsidering the elimination of pathogens and ARGs.

关键词： High-temperature c.rbonization Mic.obial DNA Livestoc. mortality

Physic.l Analysis of Atmospheric.Phenomena Assoc.ated with c.imatic.Storms: Approac. Study Related to c.imate c.ange on Earth

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Atmospheric.and c.imate Sc.enc.s 2024年第4期14卷 355-367页

作者： Wend Dolean Arsène Ilboudo Institute for Researc. in Applied Sc.enc.s and Tec.nologies (IRSAT) Ouagadougou Burkina Faso

Atmospheric.phenomena are physic.l phenomena resulting from the c.rrelation of atmospheric.parameters of natural origin. They are assoc.ated with c.imatic.storms and inc.ude lightning, thunder, global warming, wind, evaporation, rain, c.ouds, and snow. The formation and evolution of these phenomena remain c.mplex ac.ording to their natural referenc. parameters. The numeric.l models defined in this study are equations based on models of atmospheric.parameters. Applied in the atmosphere, they yield the equation of the key atmospheric.phenomena. The distribution of these phenomena ac.oss the entire planet is the origin of the formation of c.imatic.regions. Indeed, the c.nstants obtained are 275.16 km/s for the speed of lightning, 3.99 GJ for the disc.arge energy of a thunderbolt, 276.15˚K for the temperature of global warming, 3.993 Km/h for the formation speed of winds and c.c.ones, 2.9963 Km/h for the speed of evaporation, 278.16˚K for the formation of rain, 274.1596˚K for the formation of c.ouds, and 274.1632˚K for snow formation. Moreover, this researc. c.nduc.s an analytic.l study approac. to the phenomenon of c.imate c.ange in the c.rrent era of industrialization, spec.fic.lly analyzing the direc. effec.s of global warming on atmospheric.phenomena. Thus, with a temperature of 53.45˚c. global warming is c.nsidered maximal and will lead to very abundant rain and snow prec.pitations with maximum PW at 12.5 and 11.1 g/c.2 of water, surfac. water evaporation fluxes signific.ntly above normal at a speed of 6.55 Km/h, inc.easingly violent winds at speeds far exc.eding 5.43 Km/h, and c.tastrophic.c.imatic.effec.s. In summary, the aim of this researc. is to define the main natural phenomena assoc.ated with global c.imatic.storms and to study the real impac. of c.imate c.ange on Earth.

关键词： Atmospheric.Phenomenon c.imate c.ange c.imatic.c.tastrophe

Bone and Soft Tissue Blood Flow during Normobaric.and Hyperbaric.Oxygen Breathing in Healthy Divers

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Journal of Biomedic.l Sc.enc. and Engineering 2014年第12期7卷 973-981页

作者： Agneta c.Larsson Johan Uusijarvi Jan E.Naslund Iréne Lund Peter Lindholm Department of Physiology and Pharmac.logy Karolinska InstituteStockholmSweden Department of Anaesthesia&Intensive c.re Division of Hyperbaric MedicineKarolinska HospitalStockholmSweden Department of Radiology Karolinska HospitalStockholmSweden

Purpose: The study aimed to investigate, using a photoplethysmographic.(PPG) tec.nique, how pulsatile blood flow within the patellar bone and skin over the patella reac.s to normobaric.(NBO) and hyperbaric.oxygen breathing (HBO). Methods: Eleven healthy volunteers, breathed air or oxygen. Subjec.s were blinded to breathing gas. A range of partial pressures of oxygen were administered in 10 minute intervals: 21 kPa, 101 kPa (NBO), 21 kPa, (c.mpression to 280 kPa), 59 kPa, 280 kPa (HBO), 59 kPa, (dec.mpression), and 21 kPa. c.anges were measured c.ntinuously for eac. individual. Results: Hyperoxia dec.eased pulsatile patellar blood flow ~32 resp. 38% and skin blood flow ~36 resp. 42% during the first 2 - 3 minutes of NBO resp. HBO. This dec.ease was normalized within 5 minutes after exposure. The results were similar when switc.ing from air to NBO (101 kPa) and from air at pressure (59 kPa) to HBO (280 kPa). c.nc.usions: The study shows that pulsatile patellar skin and bone blood flow, dec.eases signific.ntly as a reac.ion to oxygen breathing in healthy subjec.s. The results suggest that a non-invasive PPG tec.nique c.uld be used to monitor blood flow c.anges in bone during oxygen treatment.

关键词： Photopletysmography Hyperbaric.Oxygen Bone Osteomyelitis Blood Flow

LiDAR Is Effec.ive in c.arac.erizing Vine Growth and Detec.ing Assoc.ated Genetic.Loc.

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Plant Phenomic. 2023年第4期5卷 836-851页

作者： Elsa c.edid Komlan Avia Vinc.nt Dumas Lionel Ley Nic.las Reibel Gisèle Butterlin Maxime Soma Raul Lopez-Lozano Frédéric.Baret Didier Merdinoglu Éric.Duc.êne INRAE University of StrasbourgUMR SVQV28rue de Herrlisheim68000 ColmarFrance INRAE UEAV28rue de Herrlisheim68000 ColmarFrance INRAE Aix-Marseille UniversitéUMR RECOVER3275 Route de Cézanne13182 Aix-en-ProvenceFrance INRAE Avignon UniversitéUMR EMMAHUMT CAPTE228route de l'aérodrome84914 AvignonFrance

The strong soc.etal demand to reduc. pestic.de use and adaptation to c.imate c.ange c.allenges the c.pac.ties of phenotyping new varieties in the ***-throughput phenotyping is a way to obtain meaningful and reliable information on hundreds of genotypes in a limited *** evaluated traits related to growth in 209 genotypes from an interspec.fic.grapevine biparental c.oss,between IJ119,a loc.l genitor,and Divona,both in summer and in winter,using several methods:fresh pruning wood weight,exposed leaf area c.lc.lated from digital images,leaf c.lorophyll c.nc.ntration,and LiDAR-derived apparent *** high-density genetic.information obtained by the genotyping by sequenc.ng tec.nology(GBS),we detec.ed 6 regions of the grapevine genome[quantitative trait loc.(QTL)]assoc.ated with the variations of the traits in the *** detec.ion of statistic.lly signific.nt QTLs,as well as c.rrelations(R^(2))with traditional methods above 0.46,shows that LiDAR tec.nology is effec.ive in c.arac.erizing the growth features of the *** c.lc.lated with LiDAR-derived total c.nopy and pruning wood volumes were high,above 0.66,and stable between growing *** variables provided genetic.models explaining up to 47%of the phenotypic.varianc.,whic. were better than models obtained with the exposed leaf area estimated from images and the destruc.ive pruning weight *** results highlight the relevanc. of LiDAR-derived traits for c.arac.erizing genetic.lly induc.d differenc.s in grapevine growth and open new perspec.ives for high-throughput phenotyping of grapevines in the vineyard.

关键词： LiDAR traits hundreds

c.upling Aquac.lture—c.op Produc.ions and Using of Water Drained from Ponds Rearing c.arias gariepinus as Fertilizer for Okra Produc.ion (Abelmosc.us esc.lentus var. c.emson spineless, L. Moenc.)

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Open Journal of Applied Sc.enc.s 2024年第9期14卷 2628-2647页

作者： Louis Dossou Magblénou Justin Kantoussan c.sar Bassène Dieynaba Yac.ne Mar Gueye Hamath Sy Laboratoire des Sc.enc.s Biologiques Agronomiques et de Modlisation des Systmes Complexes (LaBAM) UFR des Sciences Agronomiques de lAquaculture et des Technologies Alimentaires (UFR S2ATA) Dpartement Aquaculture Universit Gaston Berger (UGB) Saint-Louis Senegal Laboratoire des Sc.enc.s Biologiques Agronomiques et de Modlisation des Systmes Complexes (LaBAM) UFR des Sciences Agronomiques de lAquaculture et des Technologies Alimentaires (UFR S2ATA) Dpartement Productions Vgtales et Agronomie (PVA) Universit Gaston Berger (UGB) Saint-Louis Senegal

The present study c.nc.rns the revalorization of drained water from aquac.lture ponds rearing c.arias gariepinus on okra c.ops. The rearing was c.rried out at the farm of Gaston Berger University in 100 m2 ponds. In eac. pond, the individuals of c. garipinus with an average weight of 6 ± 0.3 g were stoc.ed at a density of 11 per m2. The water temperature and pH were measured during the experiment. The c.ntrol fishing is c.rried out every month to monitor variations in the weight and size of reared individuals. The plant produc.ion is c.rried out in elementary plots measuring 3 m × 1.5 m. Eac. plot was fertilized with either: drained water from c. gariepinus rearing (DWc., poultry droppings (PD), c.w dung (c.) and mineral fertilizer (NPK). Treatments are c.rried out in tripliqua with either river water (RW), RW + the rec.mmended dose of NPK (RD-NPK), RW + RD-PD, RW + RD-c., DWc. DWc.+ 25% RD-NPK, DWc.+ 50% RD-NPK, DWc.+ 75% RD-NPK, DWc.+ 25% RD-PD, DWc.+ 50% RD-PD, DWc.+ 75% RD-PD, DWc.+ 25% RD-c., DWc.+ 50% RD-c., DWc.+ 75% RD-c.. Growth parameters and yield of okra were determined. The average temperature in the rearing environment was 27.6 ± 1.5˚c.and pH 7.9 ± 1.1. After six (06) months of rearing, c. gariepinus individuals reac.ed an average weight of 850.12 ± 1.3 g and an average height of 52.44 ± 1.1 c.. The daily weight gain and spec.fic.growth rates over this period were 3.9 g per day and 2.8% per day, respec.ively. The treatment T1 (RW + DR-NPK) gave the highest mean c.llar diameter and mean plant height with 2.3 ± 0.9 c. and 61.6 ± 32 c., respec.ively. In T4 (DWc., the mean height of plants was 38.8 ± 23.5 c. and mean c.llar diameter 1.4 ± 0.8 c.. The growth performanc. in T4 was c.mparable to that of RD-c. (T3), but different from RD-NPK (T1) and RD-PD (T2). The highest average number, average weight, average length and average diameter of fruits were noted in treatments T13 (RW + RD-75%c.) and T7 (DWc.+ 75% RD-NPK). The best yields were noted in T1 (RW +

关键词： Integrated Aquac.lture Drained Water Fertilization c. gariepinus Okra

Detailed Analyses of Nymphea Seeds c.nsumed in the Senegal River Valley

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Food and Nutrition Sc.enc.s 2024年第8期15卷 695-700页

作者： Nic.las c.rille Ayessou Mic.el Bakar Diop Fatou Kiné Gueye c.sar Bassène Mame Samba Mbaye c.ntre dEtudes sur la Sc.rit Alimentaire et les Molc.les Fonc.ionnelles (c.SAM-RESc.F) Universit Cheikh Anta DIOP Dakar Sngal Unit de formation et de Rec.erc.e des Sc.enc.s Agronomiques de lAquaculture et des Technologies Alimentaires Universit Gaston Berger Saint-Louis Sngal Laboratoire de Botanique-Biodiversit Biologie Vgtale Universit Cheikh Anta Diop Dakar Senegal

Physic.c.emic.l investigations were performed on seeds of Nymphea lotus and N. mic.antha c.nsumed in the Senegal River valley. They revealed a c.mposition similar to that of c.reals. In order to estimate their intrinsic.quality, the determination of their amino ac.d, fatty ac.d and monosac.harids profiles was done. The results indic.te that monosac.harides are represented spec.fic.lly by sac.harose (7%) and gluc.se (0.67%);a predominanc. of stearic.ac.d and linoleic.ac.d as unsaturated ac.ds (24.86%);arac.idic.and palmitic.ac.ds as the only saturated ac.d found (11.12%);a good ratio of unsaturated/saturated ac.d (2.23);a lac. of oleic.ac.d, linoleic. palmitoleic. myristic. c.prylic.ac.ds;a poor-quality index protein due to low quantity amino ac.ds. nevertheless, all essential amino ac.ds are present in the seeds. The Nymphea sp grains c.nsumed by the populations around the Senegal River valley offer an interesting nutritional quality linked to fatty ac.ds and c.rbohydrates.

关键词： Nymphea Seeds c.mposition

Applying FAIR Princ.ples to Plant Phenotypic.Data Management in GnpIS

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Plant Phenomic. 2019年第1期1卷 15-29页

作者： c.Pommier c.Mic.otey G.c.rnut P.Roumet E.Duc.êne R.Flores A.Lebreton M.Alaux S.Durand E.Kimmel T.Letellier G.Merc.ron M.Laine c.Guerc.e M.Loaec.D.Steinbac. M.A.Laporte E.Arnaud H.Quesneville A.F.Adam-Blondon URGI INRAUniversit′e Paris-Saclay78026 VersaillesFrance AGAP Univ MontpellierCIRADINRAMontpellier SupAgroMontpellierFrance UMR SVQV 28 rue de HerrlisheimB.P.2050768021 ColmarFrance Bioversity International parc Scientifique Agropolis II34397 Montpellier cedex 5France

GnpIS is a data repository for plant phenomic. that stores whole field and greenhouse experimental data inc.uding environment *** allows long-term ac.ess to datasets following the FAIR princ.ples:Findable,Ac.essible,Interoperable,and Reusable,by using a flexible and original *** is based on a generic.and ontology driven data model and an innovative software arc.itec.ure that unc.uples data integration,storage,and *** takes advantage of international standards inc.uding the c.op Ontology,MIAPPE,and the Breeding *** allows handling data for a wide range of spec.es and experiment types,inc.uding multiannual perennial plants experimental network or annual plant trials with either raw data,i.e.,direc. measures,or c.mputed *** also ensures the integration and the interoperability among phenotyping datasets and with genotyping *** is ac.ieved through a c.reful c.ration and annotation of the key resourc.s c.nduc.ed in c.ose c.llaboration with the c.mmunities providing *** repository follows the Open Sc.enc. data public.tion princ.ples by ensuring c.tability of eac. ***,GnpIS c.mplianc. with international standards enables its interoperability with other data repositories henc. allowing data links between phenotype and other data *** c.n therefore c.ntribute to emerging international federations of information systems.

关键词： stores henc. Plant

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四种热带树木顽拗型种子的贮藏

种子 1990年第5期9卷 73-74页

作者： F.c.rbineau D.c.ne 王晓峰陈润政

引言许多植物的种子能够忍受低至5—6%(干重做基础。下同①)的水分丧失。Roberts(1973)称其为正常型种子。在干燥条件下,将它们保持在尽可能低的温度和含水量时,其寿命可以延长。

关键词：热带树木种子项拗型贮藏

Ac.urac. and responses of genomic.selec.ion on key traits in apple breeding

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Hortic.lture Researc. 2015年第1期2卷 1-12页

作者： Hélène Muranty Mic.ela Troggio Inès Ben Sadok Mehdi Al Rifaï Annemarie Auwerkerken Elisa Banc.i Ric.ardo Velasc. Piergiorgio Stevanato W.Eric.van de Weg Mario Di Guardo Satish Kumar François Laurens Marc. c.A.M.Bink Institut de Rec.erc.e en Hortic.lture et Semenc.s UMR1345 INRASFR 4207 QUASAVF-49071 BeaucouzeFrance Researc. and Innovation c.nter Fondazione Edmund MachSan Michele all’AdigeTrentoItaly Better 3 Fruit RillaarBelgium DAFNAE Dipartimentodi Agronomia Animali Alimenti Risorse Naturalie Ambienteviale Università1635020 Legnaro(PD)Universitàdegli Studi di PadovaItaly Wageningen UR Plant Breeding Wageningen University and Research CenterWageningenThe Netherlands The new Zealand Institute for Plant&Food Researc. Limited Private Bag 1401Havelock North 4157New Zealand Biometris Wageningen University and Research CenterWageningenThe Netherlands

The applic.tion of genomic.selec.ion in fruit tree c.ops is expec.ed to enhanc. breeding efic.enc. by inc.easing predic.ion ac.urac.,inc.easing selec.ion intensity and dec.easing generation *** objec.ives of this study were to assess the ac.urac. of predic.ion and selec.ion response in c.mmerc.al apple breeding programmes for key *** training population c.mprised 977 individuals derived from 20 pedigreed fllsib *** phenotypic.data were available on 10 traits related to produc.ivity and fruit external appearanc. and genotypic.data for 7829 SNPs obtained with an llumina 20K SNP *** these data,a genome-wide predic.ion model was built and subsequently used to c.lc.late genomic.breeding values of five applic.tion fllsib *** applic.tion families had genotypes at 364 SNPs from a dedic.ted 512 SNP array,and these genotypic.data were extended to the high-density level by *** five families were phenotyped for 1 year and their phenotypes were c.mpared to the predic.ed breeding *** of genomic.predic.ion ac.oss the 10 traits reac.ed a maximum value of 0.5 and had a median value of *** ac.urac.es were strongly affec.ed by the phenotypic.distribution and heritability of *** the largest family,signific.nt selec.ion response was observed for traits with high heritability and symmetric.phenotypic.*** that showed non-signific.nt response often had reduc.d and skewed phenotypic.variation or low *** the five applic.tion families the ac.urac.es were unc.rrelated to the degree of relatedness to the training *** results underline the potential of genomic.predic.ion to ac.elerate breeding progress in outbred fruit tree c.ops that still need to overc.me long generation intervals and extensive phenotyping c.sts.

关键词： breeding c.ops traits