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Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source,Talaromyces purpureogenus KJ584844

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BIOCELL 2021年第1期45卷 119-127页

作者： SALLY NEGM MOHAMMAD EL-METWALLY wesam eldin saber SAHAR ABO-NEIMA MAHMOUD MOUSTAFA ATTALLA EL-KOTT Life Sciences Department College of Science and literature Mahyel AseerKing Khalid UniversityAbhaSaudi Arabia Unit of Food Bacteriology Central Laboratory of Food HygieneMinistry of HealthSharkiaEgypt Department of Botany and Microbiology Faculty of ScienceDamanhour UniversityEl-BeheraEgypt Microbial Activity Unit Department of MicrobiologySoilsWater and Environment Research InstituteAgricultural Research CenterGizaEgypt Physics Department Faculty of ScienceDamanhour UniversityDamanhourEgypt Department of Biology College of ScienceKing Khalid University9004AbhaSaudi Arabia Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQenaEgypt Zoology Department Faculty of ScienceDamanhour UniversityEl-BeheraEgypt

The need for the bacterial proteinase is rapidly growing,urging to catch a lowcost medium for the microbial fermentation,nanoparticles can play a vital role in this *** proteinase of Talaromyces purpureogenus was produced on the tubers of Helianthus tuberosus that also operated as solid support for the fermentation *** interface amongst nitrogen sources(NH_(4)Cl and yeast extract)was investigated,applying the statistical modeling of central composite design under solid-state *** optimum medium for proteinase secretion was stimulated by 979.82 mg NH_(4)Cl and 437.68 mg yeast extract per 100 g substrate,yielding 108.15 U/g *** Plackett-Burman experimental design,the nanoparticles Co,Ni and Fe were assessed as inducers for proteinase *** nanoparticles(5 ppm)were the greatest in both proteinase production by the fungus as well as an inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the reaction mixture,liberating the extreme quantity of amino acids,compared with the lack of the *** findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during the degradation of proteinaceous materials induce proteinase *** approach could be extended to modulate the productivity and activity of similar biomolecules.

关键词： Experimental design Helianthus tuberosus proteolysis solid-state fermentation

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