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Standardized high-throughput biomonitoring using DNA metabarcoding: Strategies for the adoption of automated liquid handlers

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Environmental Science and Ecotechnology 2021年第4期8卷 22-30页

作者： Dominik Buchner Till-Hendrik Macher Arne J.Beermann Marie-Therese werner Florian Leese University of Duisburg-Essen Aquatic Ecosystem ResearchUniversitatsstr.545141EssenGermany University of Duisburg-Essen Centre for Water and Environmental Research(ZWU)Universitatsstr.345141EssenGermany

Reliable and comprehensive monitoring data are required to trace and counteract biodiversity loss. Highthroughput metabarcoding using DNA extracted from community samples (bulk) or from water orsediment (environmental DNA) has revolutionized biomonitoring, given the capability to assess biodiversity across the tree of life rapidly with feasible effort and at a modest price. DNA metabarcoding canbe upscaled to process hundreds of samples in parallel. However, while automated high-throughputanalysis workflows are well-established in the medical sector, manual sample processing still predominates in biomonitoring laboratory workflows limiting the upscaling and standardization for routinemonitoring applications. Here we present an automated, scalable, and reproducible metabarcodingworkflow to extract DNA from bulk samples, perform PCR and library preparation on a liquid *** features are the independent sample replication throughout the workflow and the use of manynegative controls for quality assurance and quality control. We generated two datasets: i) a validationdataset consisting of 42 individual arthropod specimens of different species, and ii) a routine monitoringdataset consisting of 60 stream macroinvertebrate bulk samples. As a marker, we used the mitochondrialCOI gene. Our results show that the developed single-deck workflow is free of laboratory-derivedcontamination and produces highly consistent results. Minor deviations between replicates are mostlydue to stochastic differences for low abundant OTUs. Thus, we successfully demonstrated that roboticliquid handling can be used reliably from DNA extraction to final library preparation on a single deck,thereby substantially increasing throughput, reducing costs, and increasing data robustness for biodiversity assessments and monitoring.

关键词： Pipetting robot Automatization Standardization High-throughput sequencing

Sclerosing angiomatoid nodular transformation of the spleen, a rare cause for splenectomy: Two case reports

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World Journal of Clinical Cases 2020年第1期8卷 103-109页

作者： Sophia Chikhladze Ann-Kathrin Lederer Stefan Fichtner-Feigl Uwe A Wittel Martin werner Konrad Aumann Department of General and Visceral Surgery Medical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburg 79106Germany Center for Complementary Medicine Department of Infection Prevention and Hospital EpidemiologyMedical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburg 79106Germany Institute of Surgical Pathology Medical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburg 79106Germany

BACKGROUND Sclerosing angiomatoid nodular transformation(SANT)is a rare benign disease of the spleen with unknown *** symptoms are inhomogeneous,and suspicious splenic lesion often found incidentally,leading to splenectomy,as malignancy cannot securely be ruled *** is made histologically after *** SUMMARY Two cases of German,white,non-smoking,and non-drinking patients of normal weight are *** first one is a 26-year-old man without medical history who was exhibiting an undesired weight loss of 10 kg and recurring vomiting for about 18 *** second one is a 65-year-old woman with hypertension who had previously undergone gynecological surgery,suffering from a lasting feeling of abdominal *** showed radiologically an inhomogeneous splenic lesion leading to splenectomy approximately 6 and 9 wk after surgical *** diagnoses of SANT were made ***-up went well,and both were treated according to the recommendation for asplenic *** SANT is a rare cause of splenectomy and an incidental histological *** research should focus on clinical and radiological diagnosis of SANT as well as on treatment of patients with asymptomatic and small findings.

关键词： Splenectomy Sclerosing angiomatoid nodular transformation Spleen Abdominal discomfort Gastrointestinal dysfunction Case report

Mathematical Rotordynamic Model Regarding Excitation Due to Elliptical Shaft Journals in Electrical Motors Considering the Gyroscopic Effect

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Applied Mathematics 2013年第8期4卷 57-74页

作者： Ulrich werner Siemens AG Industry Drive Technologies Large Drives Products Research & Development Nuremberg Germany

The paper presents a mathematical rotordynamic model regarding excitation due to elliptical shaft journals in sleeve bearings of electrical motors also considering the gyroscopic effect. For this kind of excitation, a mathematical rotordynamic model was developed considering the influence of the oil film stiffness and damping of the sleeve bearings, the stiffness of the end-shields and bearing housings, the stiffness of the rotor, the electromagnetic stiffness in the air gap of the electrical motor and the mass moment of inertia of the rotor and therefore also considering the gyroscopic effect. The solution of the linear differential equation system leads to the mathematical description of the absolute orbits of the shaft centre, the shaft journals and the bearing housings and to the relative orbits between the shaft journals and the bearing housings. Additionally, the bearing housing velocities can also be derived with this mathematical rotordynamic model.

关键词： Rotordynamics Elliptical Shaft Journals Orbit Vibrations

Mathematical Multibody Model of a Soft Mounted Induction Motor Regarding Forced Vibrations Due to Dynamic Rotor Eccentricities Considering Electromagnetic Field Damping

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Journal of Applied Mathematics and Physics 2017年第2期5卷 346-364页

作者： Ulrich werner Faculty of Electrical Engineering Precision Engineering Information Technology Georg Simon Ohm University of Applied Sciences Nuremberg Germany

The paper presents a mathematical multibody model of a soft mounted induction motor with sleeve bearings regarding forced vibrations caused by dynamic rotor eccentricities considering electromagnetic field damping. The multibody model contains the mass of the stator, rotor, shaft journals and bearing housings, the electromagnetic forces with respect of electromagnetic field damping, stiffness and internal (rotating) damping of the rotor, different kinds of dynamic rotor eccentricity, stiffness and damping of the bearing housings and end shields, stiffness and damping of the oil film of the sleeve bearings and stiffness and damping of the foundation. With this multibody model, the bearing housing vibrations and the relative shaft vibrations in the sleeve bearings can be derived.

关键词： Induction Motor Electromagnetic Field Damping Rotordynamics Soft Mounting

Effect of Chlorophytum Borivilianum Santapau and Fernandes on Sexual Dysfunction in Hyperglycemic Male Rats

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Chinese Journal of Integrative Medicine 2009年第6期15卷 448-453页

作者： Mayank Thakur Shilpi Bhargava werner Praznik Renate Loeppert Vinod K Dixit Department of Pharmaceutical Sciences Dr. Hari Singh Gour University Department of Chemistry University of Natural and Applied Life Sciences Dr.Hari Singh Gour University

Objective:To investigate the effects of Chlorophytum borivilianum(CB) on sexual dysfunction, loss of body weight,and lack of libido in hyperglycemic rats induced with streptozotocin or ***: Wistar strain male albino rats were divided into five groups of six animals each:the control group(2% polyvinylpyrollidone solution),the streptozotocin control group(50 mg/kg),the alloxan control group(100 mg/kg), the streptozotocin + CB treated group(200 mg/kg),and the alloxan + CB treated group(200 mg/kg).O...

关键词： ayurvedic rasayana Chlorophytum borivilianum diabetes induced sexual dysfunction Safed Musli

The clinical rehabilitation of spine and spinal cord disorders: detection and evaluation using SPECT/CT

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Neural Regeneration Research 2014年第8期9卷 795-797页

作者： Max J. Scheyerer Clément M.L.werner Patrick Veit-Haibach Centre for Orthopaedic and Trauma Surgery University Medical CenterJosef-Stelzmann-Str.9CologneGermany Department of Surgery Division of TraumaSurgeryUniversity Hospital ZurichZurichSwitzerland Department of Medical Radiology Division of Nuclear MedicineUniversity Hospital ZurichZurichSwitzerland Department of Medical Radiology Diagnostic and Inverventional Radiolo-gyUniversity Hospital ZurichZurichSwitzerland

Spine-related disorders are caused by several factors including （1） spinal nerve/visceral nerve stimulation by perivertebral aseptic inflammation, （2） spinal nerve/visceral nerve compression by injured periverterbal soft tissue, dislocated perivertebral small joint, and proliferative/degenerative tissue and, （3） secondary damage to the spinal cord, peripheral nerve, vessels and autonomic nerve, which further stimulate nerve root sheath and surround- ing pain-carrying nerve fibers. In many cases, the source of pain cannot be detected by standard image modalities. Particularly in anatomically complex regions like the spine, SPECT/CT can be helpful for some aspects by intro- ducing a metabolical dimension to the classical way of morphology-based diagnostic. The aim of the present review was to give an overview of the adoption of SPECT/CT in a clinical spine-focused setting.

关键词： SPECT The clinical rehabilitation of spine and spinal cord disorders detection and evaluation using SPECT/CT

Price Drop of Pyrethroid Insecticides

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China Chemical Reporter 2009年第16期20卷 32-32页

作者： John werner the Pesticide Market Information Center

Due to impacts from the global financialcrisis, the demand for pyrethroid insecticides in overseas markets is down. Theamount of pyrethroid insecticides exportedby domestic producers declined notablyfrom January to April 2009. Few purchaseorders were made at the China InternationalAgrochemical & Crop Protection Exhibitionheld in Shanghai in March 2009. As a resultproducts are mainly sold in the domesticmarket. As pests were not serious thisspring, the demand for pyrethroid insecticides in China is slack. Fierce competitionbetween producers has led to a *** ex-factory price of 95%

关键词： factory declined today overseas registered slightl

Dependence of Gravity Induced Absorption Changes on the Earth’s Magnetic Field as Measured during Parabolic Flight Campaigns

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Journal of Modern Physics 2013年第11期4卷 1546-1553页

作者： werner Schmidt Fachbereich Biologie Philipps-Universitat MarburgMarburgGermany Fachbereich Biologie Universitat KonstanzKonstanzGermany

Various spectroscopic experiments performed on the AIRBUS ZERO G—located in Bordeaux, France—in the years 2002 to 2012 exhibit minute optical reflection/absorption changes (GIACs) as a result of gravitational changes between 0 and 1.8 g in various biological species such as maize, oats, Arabidopsis and particularly Phycomyces sporangiophores. During a flight day, the AIRBUS ZERO G conducts 31 parabolas, each of which lasts about three minutes including a period of 22 s of weightlessness. So far, we participated in 11 parabolic flight campaigns including more than 1000 parabolas performing various kinds of experiments. During our campaigns, we observed an unexplainable variability of the measuring signals (GIACs). Using GPS-positioning systems and three dimensional magnetic field sensors, these finally were traced back to the changing earth’s magnetic field associated with the various flight directions. This is the first time that the interaction of gravity and the Earth’ magnetic field in the primary induction process in living system has been observed.

关键词： MDWS(Micro Dual Wavelength Spectrometer) GIAC(Gravity Induced Absorption Change) AIRBUS-300-ZERO-G Parabolic Flight Micro-and Hypergravity Three Dimensional Earth’s Magnetic Field Global Positioning System(GPS) Google Earth

Gravireception in <i>Phycomyces</i>: Threshold Determination on the Sounding Rocket TEXUS 50

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Journal of Modern Physics 2015年第10期6卷 1381-1389页

作者： werner Schmidt Fachbereich Biologie Philipps-Universit&#228 t Marburg Marburg Germany Fachbereich Biologie Universit&#228 t Konstanz Konstanz Germany

Under parabolic flight conditions microgravity is not lower than 3 to 5 times 10-2 g. In contrast to parabolic flights, sounding rocket flights are virtually vibrational-free allowing microgravity as low as 10-5 g. Thus, a rotating platform serving as centrifuge allows the precise generation of gravitational forces ranging from 5 to 100 mg (not possible during parabolic flights). On this basis we determined the threshold1 for optical reflection/absorption changes in Phycomyces to be lower than 25 × 10-3 g. This compares well with the threshold determination of gravitropism in Phycomyces on a clinostat centrifuge. Kinetics of gravity-induced absorption changes and gravity as generated by the on-board centrifuge do not coincide but show a distinctive hysteresis with a latency of 4 s (75 mg-ramp, pull-up).

关键词： MDWS (Micro-Dual Wavelength Spectrophotometer) Δ GIAC (Differential Gravity-Induced Absorption Change) Phycomyces blakesleeanus Sporangiophore Micro- and Hypergravity Texus 50 Sounding Rocket Gravireception