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Geochemical characteristics and diagenetic fluids of dolomite reservoirs in the Huanglong Formation, Eastern Sichuan Basin, China

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Petroleum Science 2014年第1期11卷 52-66页

作者： wen huaguo wen Longbin Chen Haoru Zheng Rongcai Dang Lurui Li Yanan State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of Technology Institute of Sedimentary Geology Chengdu University of Technology Chongqing Gas District of Southwest Oil and Gas Company of CNPC

Based on a comprehensive study of texture,diagenetic behavior and evolution of dolomite in the Huanglong Formation,trace （e.g.,Fe,Mn and Sr） and rare earth element （REE） geochemistry,andisotopic characteristics （e.g.,C,O and Sr）,four types of diagenetic fluids are identified in the Huanglong Formation dolomite reservoirs of the Eastern Sichuan Basin,China：1）：marine-derived pore waters in the marine diagenetic environment,2） sabkha compaction brine conserved in the early shallowburied diagenetic environment,3） strongly-oxidizing low-temperature meteoric water in the seepagesubsurface flow diagenetic environment,and 4） strongly reducing deeply seated mixed hot brine in the middle and deep burial diagenetic environment.The fluids developed hereditarily from one environment to another,which resulted in its respective characteristics.Fluid characteristics play an important role in the development of dolomite reservoirs：1） dolomitization by marine-derived pore water in the quasisyngenetic stage did not form an effective reservoir; 2） early diagenetic burial dolomitization by the sabkha compaction brine formed the basis for reservoir development; 3） meteoric water karstification in the paleo-epidiagenetic stage expanded both the distribution and the size of the reservoirs,and improved the reservoir quality; 4） deep-burial dissolution and tectonic fracturing in the reburial diagenetic stage further improved reservoir porosity and permeability.

关键词： Dolomite reservoir geochemistry diagenetic fluids Huanglong Formation Carboniferous Sichuan Basin

The Origin of Paleokarst in the Huanglong Formation of the Eastern Sichuan Basin:Evidence from δ^(13)C, δ^(18)O and ^(87)Sr/^(86)Sr

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Acta Geologica Sinica(English Edition) 2014年第6期88卷 1852-1862页

作者： CHEN Haoru ZHENG Rongcai wen huaguo LI Shilin LI Shuang ZHOU Gang wen Longbin State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of Technology Chongqing Gas District of Southwest Oil and Gas Company of CNPC Petroleum Exploration and Development Research Institute PetroC hina Southwest Oil and Gasfield Company

Karst rocks from the Huanglong Formation exposed at the margin of the Eastern Sichuan Basin can be divided into four types：slightly corroded, moderately corroded porous, intensely corroded brecciated and intensely corroded and replaced secondary calcic karstic rocks. The carbon, oxygen and strontium isotope compositions of the various karst rocks are analyzed systematically and compared to rocks without karst corrosion. The results indicate that（1） the Huanglong Formation in the eastern Sichuan Basin was a restricted bay supplied and controlled by freshwater in which mudmicrite and mud-dolomicrite exhibit low δ13C and δ18O values and high 87Sr/86 Sr ratios;（2） all types of karstic rocks in the paleokarst reservoirs of the Huanglong Formation in the research area are affected by atmospheric freshwater with the δ13C and δ18O values and 87Sr/86 Sr ratios in the original formation approaching those of atmospheric freshwater, which reflects ancient hydrological conditions, fluid properties, isotopic source and the fractionation effect;（3） the intensely corroded and replaced secondary limestone is affected by a variety of diagenetic fluids, often reflected by δ13C and δ18O values, while the 87Sr/86 Sr ratios exhibit the strong degree of the corrosion;（4） after comparing the 87Sr/86 Sr ratios of each type of karst rock, the diagenetic fluids are determined to be mainly atmospheric freshwater, and depending on the strength of corrosion, and the low 87Sr/86 Sr ratio fluids in the layer will participate in the karst process. The carbon, oxygen, and strontium isotopes of different karstic reservoirs can provide meaningful geochemical information for forecasting and evaluating the development and distribution rules of the Huanglong Formation at the margin of the eastern Sichuan Basin in time and space.

关键词： paleokarst reservoirs,carbon and oxygen isotope,strontium isotope,geochemistry,Huanglong Formation,eastern Sichuan area

Primary dolostone related to the Cretaceous lacustrine hydrothermal sedimentation in Qingxi sag, Jiuquan Basin on the northern Tibetan Plateau

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Science China Earth Sciences 2013年第4期56卷 2080-2093页

作者： wen huaguo ZHENG RongCai QING HaiRuo FAN MingTao LI YanAn GONG BoShi State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of Technology Institute of Sedimentary Geology Chengdu University of Technology Department of Geology University of Regina Yumen Oilfield Company of PetroChina

Based on comprehensive studies in petrography,petrofabric analysis and geochemistry,this paper describes a unique and rare laminated micritic ferruginous primary dolostone crystallized and precipitated from the alkaline hot brine under the conditions of the Mesozoic faulted lake basin.The main rock-forming mineral of this dolostone is ferruginous dolomite with a micritic structure.This dolomite mostly exhibits laminae of 0.1–1 mm thick and is often discovered with other minerals,such as albite,analcite,barite and dickite,which have at least two types of interbedded laminae.Petrogeochemistry reveals that this dolostone contains a large number of typomorphic elements of hydrothermal sedimentation,including Sb,Ba,Sr,Mn,and V.In addition,the LREE is in relatively high concentrations and possesses the typical REE distribution pattern with negative Eu anomaly.Oxygen isotope values(δ18OPDB)range from 5.89‰to 14.15‰with an average of 9.69‰.The ratio of87Sr/86Sr is between0.711648 and 0.719546,with an average of 0.714718.These data indicate that the depositional environment is a stable,blocked,anoxic low-lying hot brine pool in the bottom of deep lake controlled by basement faults.The hydrothermal fluid is the alkaline hot brine formed by the combination of the infiltration lake water and mantle-derived magmatic water,consisting of many ions,including Ca2+,Mg2+and Fe2+.Under the driving flow power of magmatic heat,gravity and compaction,the hydrothermal fluid overcame the overburden pressure and hydrostatic pressure of the lake water body,and boiled to explosion,and then the explosion shattered the original laminated micritic ferruginous primary dolostone near the vent and then formed a new type of dolostone called shattered"hydroexplosion breccias".In the low-lying,unperturbed hot brine pool,far from the vent,the laminated micritic ferruginous primary dolostone was quickly crystallized and chemicals precipitated from the hydrotherm.This study of special rocks contributes to research into the causes of the formation of la

关键词：湖相白云岩湖相碳酸盐岩藏北高原热水沉积酒泉盆地白垩系岩石地球化学下垂

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5种枸杞多糖生物活性的比较研究

中国中药杂志 2024年第1期49卷 110-122页

作者：王生琳宰清勇陈华国李佳文周欣贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室贵州贵阳550001 贵州省药物质量控制及评价技术工程实验室贵州贵阳550001

枸杞多糖(Lycium barbarum polysaccharides, LBPs)作为枸杞子中重要的活性成分,其理化性质和生物活性的研究具有重要意义。该研究采用前期通过不同提取方法(冷水浸提、冷水浸提后的滤渣沸水回流提取、50%乙醇超声提取、50%乙醇提取后... 详细信息

枸杞多糖(Lycium barbarum polysaccharides, LBPs)作为枸杞子中重要的活性成分,其理化性质和生物活性的研究具有重要意义。该研究采用前期通过不同提取方法(冷水浸提、冷水浸提后的滤渣沸水回流提取、50%乙醇超声提取、50%乙醇提取后的滤渣25%乙醇超声提取、热水浸提)得到的5种LBPs(LBP-1、LBP-2、LBP-3、LBP-4、LBP-5)为研究对象,利用紫外光谱、热重分析、扫描电镜对LBPs的结构进行了表征,并比较了其体外生物活性(抗氧化、降血脂、抑制亚硝化反应、抑制乙酰胆碱酯酶和抑制酪氨酸酶活性)。结果表明,高温提取会破坏多糖的结构,而超声辅助提取保证了多糖主要结构的完整;5种LBPs的热稳定性和降解行为存在差异,但5种LBPs的紫外光谱分析结果并无显著差异,都显示蛋白质的特征吸收峰。生物活性结果表明,LBP-3和LBP-4表现出良好的抗氧化活性,而LBP-3降血脂活性最好。此外,LBP-3对亚硝化反应和乙酰胆碱酯酶的抑制作用也显著优于其他组分。相反,LBP-2对酪氨酸酶的抑制能力最为出色。该文探讨了不同提取方法对枸杞多糖的理化性质和生物活性的影响,以期为选择合适的提取方法以获得具有理想生物活性的枸杞多糖提供科学依据。

关键词：枸杞多糖结构生物活性比较研究

Occurrence of Laminated Dolomitic Exhalative Rocks in Continental Rift Basins of Northwest China

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Acta Geologica Sinica(English Edition) 2017年第5期91卷 1934-1935页

作者： wen huaguo LUO Lianchao ZHENG Rongcai Hairuo QING XU wenli State Key Laboratory of oil and Gas Reservoir Geology and Exploitation Chengdu University of Technology Chengdu 610059 Sichuan China Institute of Sedimentary Geology Chengdu University of Technology Chengdu 610059 Sichuan China Department of Geology University of Regina Regina SK S4SOA2 Canada

Objective Researchers have recently discovered that sublacustrine sedimentary exhalative mechanism associated with volcanism,is the principle way to form lacustrine exhalative rocks.These rocks differentiate themselve... 详细信息

关键词： Occurrence of Laminated Dolomitic Exhalative Rocks in Continental Rift Basins of Northwest China REE

Geochemical characteristics and diagenetic systems of dolomite reservoirs of the Changxing Formation in the eastern Sichuan Basin, China

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Petroleum Science 2012年第2期9卷 141-153页

作者： Zhang Bing Zheng Rongcai Wang Xuben Zheng Chao wen huaguo Luo Yuan Chi Yuelong State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Engineering Chengdu University of TechnologySichuan 610059China Chongqing Gas Mine PetroChina Southwest Oil and Gasfield CompanyChongqing 401120China Sichuan Science and Technology Worker University Sichuan 610059China

In order to discuss the relationship between dolomite reservoirs and diagenetic systems of the Changxing Formation, we studied carbon, oxygen and strontium stable isotopes, iron, manganese and strontium trace elements and the Mg/Ca （mol%） ratio, dolomite order degree, and determined that burial dolomitization is the key to controlling the distribution of high quality dolomite reservoir in the Changxing Formation in the eastern Sichuan Basin. The dolomite of the Changxing Formation is divided into four diagenetic systems：（1） penecontemporaneous stage syngenetic brine diagenetic system, （2） early diagenetic stage strata seal brine diagenetic system, （3） middle-late diagenetic stage mixed hot brine diagenetic system and （4） tectonic uplift stage mixed hydrothermal fluid diagenetic system. New understanding of the controlling factors and distribution of dolomite reservoir development is discussed. Reef shoal facies belts controlled regional reservoir distribution and the scale of development. Burial dolomitization of a strata seal brine diagenetic system is the foundation of reservoir development, mainly developing pore reservoir. Burial dolomitization of mixed hot brine diagenetic system expanded the reservoir distribution and improved the reservoir quality, mainly developing pore-vug reservoir. Fracturing and dissolution of a mixed hydrothermal fluid diagenetic system is the key to improving the reservoir quality, mainly developing pore-vug-crack complex reservoirs.

关键词： Eastern Sichuan Basin Changxing Formation dolomite reservoir diagenesis geochemical characteristics diagenetic system

川东五百梯地区石炭系岩溶古地貌识别及储集意义

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岩性油气藏 2024年第4期36卷 109-121页

作者：王同川陈浩如温龙彬钱玉贵李玉琢文华国油气藏地质及开发工程全国重点实验室成都理工大学成都610059 成都理工大学沉积地质研究院成都610059 西华师范大学环境科学与工程学院四川南充637009 中石化西南油气分公司勘探开发研究院成都610041

综合利用钻井、测井、岩心及地震资料,通过建立地震正演模型模拟、RGB分频融合技术及波形分类分析等手段,对川东五百梯地区石炭系岩溶古地貌进行了识别与刻画。研究结果表明:(1)川东五百梯地区石炭系遭受的岩溶作用强度自西向东逐渐变小... 详细信息

综合利用钻井、测井、岩心及地震资料,通过建立地震正演模型模拟、RGB分频融合技术及波形分类分析等手段,对川东五百梯地区石炭系岩溶古地貌进行了识别与刻画。研究结果表明:(1)川东五百梯地区石炭系遭受的岩溶作用强度自西向东逐渐变小,东南部石炭系原始沉积厚度更大,但残余厚度分布不均匀,主要为10~30 m;西北部地层厚度较小,为0~15 m。(2)研究区石炭系自西向东可识别出岩溶高地和岩溶斜坡2个二级地貌单元,岩溶斜坡可划分为残丘和浅洼2个三级地貌单元,再依据其差异溶蚀特征,可进一步划分为陡斜坡浅洼和缓斜坡浅洼,浅洼内发育残丘、暗河及落水洞。反射同相轴的上提和下拉特征、岩溶角砾岩的发育、地震层拉平剖面、RGB分频融合及波形变化特征等均可识别出岩溶古地貌的发育。(3)研究区石炭系各地貌单元的分布受构造控制,浅洼和残丘多以椭圆状或不规则长条状呈北西—南东向展布,暗河的展布与浅洼长轴方向一致;岩溶古地貌控制着地层厚度以及溶蚀孔洞的发育,残丘残余厚度较大,岩溶作用强,充填作用较弱,溶蚀孔洞较发育,储层平均孔隙度为4.34%;缓斜坡浅洼残余厚度较小,充填作用较强,溶蚀孔洞不发育,储层平均孔隙度为2.68%,而陡斜坡浅洼虽然残余厚度小,但因潜水位低、泄水能力强,溶蚀作用强而充填作用弱,储集空间发育,储层平均孔隙度为5.05%;缓斜坡和陡斜坡储层平均孔隙度分别为3.67%和3.31%,岩溶高地孔隙度最小,平均为2.51%。

关键词：岩溶古地貌浅洼残丘岩溶斜坡带地震正演模拟 RGB分频融合岩溶角砾岩黄龙组石炭系川东五百梯地区

南羌塘坳陷中侏罗统布曲组含油白云岩储层特征

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矿物岩石 2024年第2期44卷 194-203页

作者：谢渝史建南文华国付小东宋春彦王家钦韩灏成都理工大学沉积地质研究院四川成都610059 自然资源部沉积盆地与油气资源重点实验室四川成都610218 中国石油天然气股份有限公司杭州地质研究院浙江杭州310023

针对南羌塘坳陷中侏罗统布曲组白云岩含油信息丰富,该层位的储集特征不够清晰现状,本次研究利用露头及岩心样品从染色薄片、铸体薄片、孔渗测试及包裹体显微荧光、均一温度等多方面展开了岩石学特征、孔渗特征、流体包裹体及成岩作用的... 详细信息

针对南羌塘坳陷中侏罗统布曲组白云岩含油信息丰富,该层位的储集特征不够清晰现状,本次研究利用露头及岩心样品从染色薄片、铸体薄片、孔渗测试及包裹体显微荧光、均一温度等多方面展开了岩石学特征、孔渗特征、流体包裹体及成岩作用的研究。研究结果表明:1)南羌塘坳陷中侏罗统布曲组至少发育5类白云岩,即粉晶、细晶、中晶、粗晶以及不等晶白云岩;2)孔隙和裂缝极为发育,其孔渗特征表明南羌塘坳陷布曲组中侏罗含油白云岩为中孔中渗储层;3)含油白云岩中的油气包裹体主要发育有2期,分布于白云岩、方解石内和晚期充填方解石脉内,早期主要分布于粉、细晶白云岩中,成熟度中等,荧光呈蓝绿色,同期含烃盐水包裹体均一温度为45~75℃;晚期要分布于中、粗晶白云岩中,成熟度较高,荧光呈蓝色,同期含烃盐水包裹体均一温度为105~120℃,有部分温度异常高,推测充注过程可能受一定热事件的影响;4)成岩作用显著,主要以压实、压溶、破裂、溶蚀以及交代作用为主,成岩作用与油气充注关系:细、粉晶白云石-油气充注-孔隙边缘鞍状白云石/白云石加大边-油气充注缝洞充填方解石-残余沥青。南羌塘坳陷中侏罗统布曲组储层特征综合研究,为该地区下一步的油气勘探具有一定的指导意义及实用价值。

关键词：含油白云岩储层特征流体包裹体布曲组南羌塘坳陷

Identification of African swine fever virus MGF505-2R as a potent inhibitor of innate immunity in vitro

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Virologica Sinica 2023年第1期38卷 84-95页

作者： huaguo Huang wen Dang Zhengwang Shi Mingyang Ding Fan Xu Tao Li Tao Feng Haixue Zheng Shuqi Xiao College of Veterinary Medicine Northwest A&F UniversityYangling712100China State Key Laboratory of Veterinary Etiological Biology College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China

African swine fever(ASF)is etiologically an acute,highly contagious and hemorrhagic disease caused by African swine fever virus(ASFV).Due to its genetic variation and phenotypic diversity,until now,no efficient commercial vaccines or therapeutic options are available.The ASFV genome contains a conserved middle region and two flexible ends that code for five multigene families(MGFs),while the biological functions of the MGFs are not fully characterized.Here,ASFV MGF505-2R-deficient mutant ASFV-Δ2R was constructed based on a highly virulent genotype II field isolate ASFV CN/GS/2018 currently circulating in China.Transcriptomic profiling demonstrated that ASFV-Δ2R was capable of inducing a larger number of differentially expressed genes(DEGs)compared with ASFV CN/GS/2018.Hierarchical clustering of up-regulated DEGs revealed that ASFV-Δ2R induced the most dramatic expression of interferon-related genes and inflammatory and innate immune genes,as further validated by RT-qPCR.The GO and KEGG pathway analysis identified significantly enriched pathways involved in pathogen recognition and innate antiviral immunity.Conversely,pharmacological activation of those antiviral immune responses by exogenous cytokines,including type I/II IFNs,TNF-αand IL-1β,exerted combinatory effects and synergized in antiviral capacity against ASFV replication.Collectively,MGF505-2R is a newly identified inhibitor of innate immunity potentially implicated in immune evasion.

关键词： African swine fever virus(ASFV) Multigene families(MGFs) MGF505-2R Differentially expressed genes(DEGs)