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Identification of key genes involved in flavonoid and terpenoid biosynthesis and the pathway of triterpenoid biosynthesis in Passiflora edulis

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Journal of Integrative Agriculture 2023年第5期22卷 1412-1423页

作者： XU Yi HUANG Dong-mei MA fu-ning YANG liu WU Bin XING wen-ting SUN Pei-guang CHEN Di XU Bing-qiang SONG shun National Key Laboratory for Tropical Crop Breeding/Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Improvement of BananasSanya Research Institute of Chinese Academy of Tropical Agricultural SciencesSanya 572000P.R.China Hainan Yazhou Bay Seed Laboratory Sanya 572000P.R.China Guangxi Crop Genetic Improvement and Biotechnology Laboratory Guangxi Academy of Agricultural SciencesNanning 530007P.R.China

Passion fruit(Passiflora edulis Sims) is a vine of the Passiflora genus in the Passifloraceae family. The extracted components include flavonoids and terpenoids, which have good anti-anxiety and anti-inflammatory effects in *** this study, we analyzed the transcriptomes of four tissues of the ‘Zixiang’ cultivar using RNA-Seq, which provided a dataset for functional gene mining. The de novo assembly of these reads generated 96 883 unigenes, among which 61 022 unigenes were annotated(62.99% yield). In addition to its edible value, another important application of passion fruit is its medicinal value. The flavonoids and terpenoids are mainly derivatives of luteolin, apigenin, cycloartane triterpenoid saponins and other active substances in leaf extracts. A series of candidate unigenes in the transcriptome data that are potentially involved in the flavonoid and terpenoid synthesis pathways were screened using homologybased BLAST and phylogenetic analysis. The results showed that the biosynthesis of triterpenoids in passion fruit comes from the branches of the mevalonate(MVA) and 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate(MEP/DOXP) pathways, which is different from the MVA pathway that is used in other fruit trees. Most of the candidate genes were found to be highly expressed in the leaves and/or flowers. Quantitative real-time PCR(qRT-PCR) verification was carried out and confirmed the reliability of the RNA-Seq data. further amplification and functional analysis of these putative unigenes will provide additional insight into the biosynthesis of flavonoids and terpenoids in passion fruit.

关键词： passion fruit RNA-Seq gene mining flavonoids terpenoids

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春灌定额对不同盐碱度农田水盐分布影响研究

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节水灌溉 2024年第4期 19-26页

作者：宋仁友吕廷波刘一凡李文昊付鑫法李港强石河子大学水利建筑工程学院新疆石河子832000 现代节水灌溉兵团重点实验室新疆石河子832000

为探究春灌定额对不同盐碱度农田土壤水盐分布的影响,确定适宜保墒洗盐的春灌定额。分别在中度盐碱地S1(5.23 g/kg)和重度盐碱地S2(8.27 g/kg)设置3种春灌定额处理(W1:1 800 m^(3)/hm^(2),W2:2 250 m^(3)/hm^(2),W3:2 700 m^(3)/hm^(2))... 详细信息

为探究春灌定额对不同盐碱度农田土壤水盐分布的影响,确定适宜保墒洗盐的春灌定额。分别在中度盐碱地S1(5.23 g/kg)和重度盐碱地S2(8.27 g/kg)设置3种春灌定额处理(W1:1 800 m^(3)/hm^(2),W2:2 250 m^(3)/hm^(2),W3:2 700 m^(3)/hm^(2)),分析比较了春灌后至棉花播种前0~100 cm土壤水盐运移和分布情况。结果表明:春灌后14~21 d,S1W2处理71.37%的水量可保持在土体内,S2W3处理77.26%水量保持在土体内,可使农田土壤达到适宜作物生长的土壤墒情;盐分淋洗主要发生在0~60 cm土层内,0~30 cm内淋洗效果更加显著,春灌定额越大将盐分带入深层的作用越强,春灌后21 d开始出现返盐现象;春灌时间在播前21 d左右为宜,中度盐碱地春灌定额2 284.7 m^(3)/hm^(2),重度盐碱地春灌定额2 700 m^(3)/hm^(2)左右时,可达到较为良好的节水保墒洗盐效果,重度盐碱地可以根据实际情况适当提高春灌定额。研究结果可为图木舒克市不同盐碱度农田春灌提供一定的理论依据和技术参考。

关键词：春灌定额盐碱度水盐运移土壤墒情盐分淋洗

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国医大师班秀文运用六经辨证理论论治妇科病思想探微

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中医药临床杂志 2024年第6期36卷 1029-1032页

作者：敖强刘文娥湖南中医药大学第一中医临床学院湖南长沙410208 湖南中医药大学第一附属医院湖南长沙410007

班秀文,国医大师,尤善于诊治妇科疾病。对于班老用六经辨证理论来指导治疗妇科疾病这一思想,目前少有进行论述的。针对这样的情况,作者在对这一思想内容深刻认识的基础上,试图围绕其思想的科学性与临床价值两个角度进行探讨。首先从中... 详细信息

班秀文,国医大师,尤善于诊治妇科疾病。对于班老用六经辨证理论来指导治疗妇科疾病这一思想,目前少有进行论述的。针对这样的情况,作者在对这一思想内容深刻认识的基础上,试图围绕其思想的科学性与临床价值两个角度进行探讨。首先从中医基础理论出发来讨论其科学性,其次从具体临床应用方法来讨论其临床价值。从而达到开拓医者临床思维和提高临床疗效的目的。通过研究得出六经辨证理论用于妇科疾病这一思想是以六经辨证为基础,以脏腑辨证和八纲辨证为纲领,同时结合妇科疾病的特点生成的。该思想完美体现了“异病同治”理论。

关键词：班秀文《伤寒论》六经辨证妇科疾病

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Hierarchical Ti_(3)C_(2)T_(x)@ ZnO Hollow Spheres with Excellent Microwave Absorption Inspired by the Visual Phenomenon of Eyeless Urchins

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Nano-Micro Letters 2022年第5期14卷 62-76页

作者： Yan‑Qin Wang Hai‑Bo Zhao Jin‑Bo Cheng Bo‑wen liu qiang fu Yu‑Zhong Wang Collaborative Innovation Center for Eco‑Friendly and Fire‑Safety Polymeric Materials(MoE) State Key Laboratory of Polymer Materials EngineeringNational Engineering Laboratory for Eco‑Friendly Polymer Materials(Sichuan)College of ChemistrySichuan UniversityChengdu 610064People’s Republic of China College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065People’s Republic of China

Ingenious microstructure design and rational composition selection are effective approaches to realize high-performance microwave absorbers,and the advancement of biomimetic manufacturing provides a new *** nature,urchins are the animals without eyes but can“see”,because their special structure composed of regular spines and spherical photosensitive bodies“amplifies”the light-receiving ***,inspired by the above phenomenon,the biomimetic urchin-like Ti_(3)C_(2)T_(x)@ZnO hollow microspheres are rationally designed and fabricated,in which ZnO nanoarrays(length:~2.3μm,diameter:~100 nm)as the urchin spines are evenly grafted onto the surface of the Ti_(3)C_(2)T_(x) hollow spheres(diameter:~4.2μm)as the urchin spherical photosensitive *** construction of gradient impedance and hierarchical heterostructures enhance the attenuation of incident electromagnetic *** the EMW loss behavior is further revealed by limited integral simulation calculations,which fully highlights the advantages of the urchin-like *** a result,the Ti_(3)C_(2)T_(x)@ZnO hollow spheres deliver a strong reflection loss of−57.4 dB and broad effective absorption bandwidth of 6.56 GHz,superior to similar *** work provides a new biomimetic strategy for the design and manufacturing of advanced microwave absorbers.

关键词： Bioinspired Hierarchical heterostructures Ti_(3)C_(2)T_(x)MXene ZnO nanoarrays Microwave absorption

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超导动态电感探测器的噪声谱分析

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物理学报 2024年第3期73卷 310-317页

作者：石中誉代旭城王浩宇麦展彰欧阳鹏辉王翼卓柴亚强韦联福刘旭明潘长钊郭伟杰舒诗博王轶文西南交通大学物理科学与技术学院量子光电实验室成都610031 南方科技大学深圳量子科学与工程研究所深圳518055 深圳国际量子研究院深圳518048 西南交通大学信息科学与技术学院信息量子技术实验室成都610031 中国科学院高能物理研究所粒子天体物理重点实验室北京100049

动态电感探测器容易频域集成,作为一种新兴的超导探测器件在(亚)毫米及光学波段的天文探测和阵列成像中得到了初步应用.在单像素层面,动态电感探测器的暗噪声水平是关键指标之一.本文详细介绍了一种适用于动态电感探测器的噪声功率谱分... 详细信息

动态电感探测器容易频域集成,作为一种新兴的超导探测器件在(亚)毫米及光学波段的天文探测和阵列成像中得到了初步应用.在单像素层面,动态电感探测器的暗噪声水平是关键指标之一.本文详细介绍了一种适用于动态电感探测器的噪声功率谱分析方法,可以较好地平衡噪声频谱分辨率与方差性能,准确且高效地进行噪声谱分析.利用此方法,研究了两种工艺的超导铝动态电感探测器,发现在铝膜上下两层镀氮化硅膜的样品的频率噪声约为裸铝样品的25%—50%.基于这种双层氮化硅工艺,进一步研究了多种几何设计的集总结构铝动态电感探测器在不同微波功率和温度下的噪声特性,实验结果与典型的二能级系统噪声行为相符.本文的研究为动态电感探测器的噪声谱表征提供了一种标准方法,并为研制低噪声的超导铝动态电感探测器奠定了基础.

关键词：动态电感探测器超导谐振器噪声谱

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Chiral N-triflylphosphoramide-catalyzed asymmetric hydroamination of unactivated alkenes: a hetero-ene reaction mechanism

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Organic Chemistry Frontiers 2022年第6期9卷 1649-1661页

作者： Na Wang Li-wen Fan Jin Zhang qiang-Shuai Gu Jin-shun Lin Guo-qiang Chen Xin-Yuan liu Peiyuan Yu College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518071China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China Department of Chemistry and Shenzhen Grubbs Institute Guangdong Provincial Key Laboratory of CatalysisSouthern University of Science and TechnologyShenzhen 518055China Academy for Advanced Interdisciplinary Studies and Department of Chemistry Southern University of Science and TechnologyShenzhen 518055China

A highly enantioselective intramolecular hydroamination reaction catalyzed by chiralN-triflylphosphoramide (NTPA) that features an exceptionally broad substrate scope of isolated unactivated alkenes was recently reported by some of us. Herein we report a detailed density functional theory(DFT) study that unveil an uncommon hetero-ene reaction mechanism for this transformation. The reaction changes from a stepwise mechanism involving discrete tertiary carbocation formation to a concertedmechanism to avoid the generation of energetically unfavorable secondary carbocation species for distinctly substituted alkene substrates. The reactivity of this reaction is primarily affected by the substituentson the internal carbon of the alkene, with the carbocation-stabilizing ones promoting the reaction. Inaddition, the reaction is also influenced by the substituents on the terminal alkene carbons: those diminishing the innate alkene polarization of the starting alkenes retard the reaction by disfavoring the formation of related transition states featuring highly polarized alkene residues. The steric effect of all thesealkene substituents was found to be largely unimportant due to the unique arrangement of the heteroene reaction complex that keeps the reacting alkene away from the sterically congested core region ofthe catalyst pocket. The lower acidity of chiral phosphoric acids than that of chiral NTPAs renders the protonated precomplex along the hetero-ene reaction pathway greatly thermodynamically disfavored. Thisindirectly results in a greater relative distortion in the corresponding transition state, which in turn causesreaction retardation. Our results demonstrate the use of intramolecular pericyclic reactions as an alternative strategy for alkene activation by chiral Brønsted acid catalysis and provide insights into the furtherdevelopment of asymmetric hydrofunctionalization of unactivated alkenes.

关键词： mechanism substituent asymmetric

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Role of Cu element in biomedical metal alloy design

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Rare Metals 2019年第6期38卷 476-494页

作者： Er-Lin Zhang Shan fu Ruo-Xian Wang Hai-Xia Li Ying liu Zhi-qiang Ma Guang-Kun liu Chen-shun Zhu Gao-Wu Qin Da-fu Chen School of Materials Science and Engineering Key Laboratory for Anisotropy and Texture of MaterialsEducation Ministry of ChinaNortheastern UniversityShenyang 110819China Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Orthopaedics and TraumatologyBeijing Jishuitan HospitalBeijing 100035China

Biomedical metals are widely used as implant materials in the human or animal body to repair organs and restore function, such as heart valves, meninges, peritoneum and artificial *** element affects the microstructure, mechanical property, corrosion resistance and wear resistance, but also influences the antibacterial and biological ***, antibacterial metal alloys have shown great potential as a new kind of biomedical materials, in which Cu has been widely used as antibacterial agent *** addition, biodegradable metal alloys, including magnesium alloy and zinc alloy, also have attracted much attention *** was also used as alloying element to adjust the degradation ***, the role of Cu in the alloy design will be very important for the development of new *** this paper, we summarized the recent research results on the Cu-containing metal alloy for biomedical application and hoped that this review would give more suggestions for the further development of biomedical metal alloy.

关键词： Cu Biomedical metal alloy Corrosion resistance Antibacterial property Biological property

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Effects of Co-addition of DBS and PEG on Wetting Performance of TiO_2 Film

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Chemical Research in Chinese Universities 2011年第1期27卷 1-5页

作者： QU Yi-chun JING Li-qiang WANG wen-xin fu Hong-gang liu Yang Key Laboratory of functional Inorganic Materials Chemistry Ministry of EducationSchool of Chemistry and Materials Science Heilongjiang University Harbin 150080 P. R. China

Nanocrystalline anatase TiO2 films with indium tin oxide（ITO） coated glass as the film substrate were fabricated through spin-coating technique. The TiO2 pastes were prepared with sodium dodecylbenzenesulfonate（DBS） modified TiO2 nanocrystals, synthesized by sol-hydrotherrnal processes in advance, together with different amounts of polyethylene glycol（PEG） macromolecules. The as-prepared films were mainly characterized by ultraviolet-visible （UV-Vis） spectroscopy, field emission scanning electron microscopy（FE-SEM） and water contact angle（WCA） measurement. Effects of the addition of DBS and PEG on the wetting performance of the resulting films and relevant mechanisms were principally investigated. The results show that the as-prepared film displays super-hydrophilic property after co-addition of DBS and PEG although it is not exposed to ultraviolet light prior to the WCA measure- ment. This appealing result is mainly attributed to the produced micro- and nanometer-scale hierarchical surface structure with uniformly dispersed micro papillae. Each papilla was made up of a great number of TiO2 nanoparticles. The possible formation mechanisms related to the DBS amphiphilic property of the characteristic surface were also suggested. Moreover, the resulting film also exhibits considerable durability in the superhydrophilicity, which is very useful for practical application in self-cleaning, anti-fogging, and bacteria-resistant fields.

关键词： Thin film Chemical synthesis Surface property

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Directional motion of dust particles at different gear structuresin a plasma

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Chinese Physics B 2020年第2期29卷 362-365页

作者： Chao-Xing dai Chao Song Zhi-Xiang Zhou wen-Tao Sun Zhi-qiang Guo fu-Cheng liu Ya-Feng He Hebei Key Laboratory of Optic-electronic Information Materials College of Physics Science and TechnologyHebei UniversityBaoding 071002China

Directional motion of dust particles in a dusty plasma ratcnet is observed *** dusty plasma ratcnet consists of two concentric gears with asymmetric *** is found that the sawtooth number affects the directional motion of dust particles along the saw *** the inerease of the sawtooth number,the particle velocity increases firstly and then decreases,and there is an optimum number of the sawtooth which could induce fast rotation of dust *** velocities of dust particles change as they are flowing along the saw *** also explore the force acting on the dust particle experimentally.

关键词： dusty plasma ratchet

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大别造山带双庙关金矿床成矿流体特征与成因

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矿物岩石地球化学通报 2024年第3期43卷 579-592页

作者：汪兴韦刘嘉李红梅朱金欧阳佳岑刘晓阳于永顺杜文洋付乐兵中国地质大学(武汉)资源学院武汉430074 湖北省地质调查院武汉430034 湖北省地质勘查工程技术研究中心武汉430034

湖北省麻城市双庙关金矿床是大别造山带中部新发现的金多金属矿床。本文在详细的野外地质调查和岩矿相学研究基础上,将成矿过程分为S1、S2、S3和S4四个阶段,其矿物组合分别为石英+钾长石±黄铁矿、石英+黄铁矿±黄铜矿、石英+黄铁矿+黄... 详细信息

湖北省麻城市双庙关金矿床是大别造山带中部新发现的金多金属矿床。本文在详细的野外地质调查和岩矿相学研究基础上,将成矿过程分为S1、S2、S3和S4四个阶段,其矿物组合分别为石英+钾长石±黄铁矿、石英+黄铁矿±黄铜矿、石英+黄铁矿+黄铜矿±金银碲化物和石英+碳酸盐,其中S3为主成矿阶段。四个成矿阶段的石英中发育富液两相(WL型)、富气两相(WV型)、含CO_(2)三相(C型)和含子矿物多相(S型)包裹体,其均一温度分别为226~445、234~422、258~392和152~215℃,盐度分别为2.8%~17.6%、2.9%~11.9%、1.9%~14.2%和1.2%~9.1%NaCl_(eqv),指示成矿流体主要为中-低温、中-低盐度和低密度的H_(2)O-CO_(2)-NaCl体系流体。S3阶段石英流体包裹体的δD_(V-SMOW)值为-89.3‰~-75.9‰,δ^(18)O_(H_(2)O)值为4.77‰~8.01‰;S2和S3阶段黄铁矿原位硫同位素数据δ^(34)S值为-3.6‰~1.6‰,指示成矿流体具有深源岩浆流体特征。综合分析认为,双庙关金矿床的成矿流体可能来源于岩浆热液,成矿过程中流体的不混溶作用是金沉淀的重要因素。

关键词：矿床成因流体包裹体岩浆热液脉型金矿床双庙关金矿床

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