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入侵红藻真江蓠(Gracilaria vermiculophylla)和非入侵红藻绳状龙须菜(Gracilariopsis chorda)的比较转录组研究

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海洋与湖沼 2024年第3期55卷 700-711页

作者：马萌向锦茜薛开学王高歌 weinberger florian 胡自民烟台大学海洋学院山东烟台264005 青岛西海岸新区海洋发展局山东青岛266427 中国海洋大学海洋生物多样性与进化研究所/海洋生命学院山东青岛266003 Marine Ecology Division GEOMAR Helmholtz-Zentrum für Ozeanforschung KielDüsternbrooker Weg 20Kiel 24105Germany

红藻真江蓠(Gracilaria vermiculophylla)是西北太平洋地区特有种,但在过去100年间它借助海运(太平洋牡蛎养殖)快速入侵到北美、欧洲和地中海等沿海栖息地,对当地的生物多样性、海洋环境和生态系统等造成重大影响。为从分子水平初步了... 详细信息

红藻真江蓠(Gracilaria vermiculophylla)是西北太平洋地区特有种,但在过去100年间它借助海运(太平洋牡蛎养殖)快速入侵到北美、欧洲和地中海等沿海栖息地,对当地的生物多样性、海洋环境和生态系统等造成重大影响。为从分子水平初步了解真江蓠成功入侵的潜在机制,文章对其入侵起源地——日本北部的真江蓠及非入侵种——绳状龙须菜(Gracilariopsis chorda)进行了同质园实验(common garden experiment)处理后的比较转录组研究,以探究该地区入侵属性不同的两种红藻间的基因表达差异。结果表明,真江蓠和绳状龙须菜共有基因序列集(Universal Gene,unigene)主要集中在核糖体、嘌呤和嘧啶代谢等通路。其中,在真江蓠中光系统II反应中心蛋白D1(photosystem II reaction center protein D1)、细胞色素P450单加氧酶(cytochrome P450 monooxygenase)和核酮糖1,5二磷酸羧化酶大亚基(Ribulose bisphosphate carboxylase large subunit,rbcL)等基因的表达量显著上调,而逆转录转座子蛋白(retrotransposon protein)、细胞壁相关的水解酶(cell wall-associated hydrolase)和金属离子转运蛋白Nramp5的表达既上调也下调。与光合作用过程相关基因的大量表达可能有助于真江蓠应对逆境胁迫,特别是光系统ⅡD1反应中心蛋白表达量升高可能有助于藻体修复光系统Ⅱ复合体,从而制造更多的有机物以备藻体生长所需。而金属离子转运蛋白Nramp5等的上调和下调则表明江蓠等红藻可能通过某些基因表达量的增减对不同的环境变动作出响应。总体而言,代谢过程中的资源再分配很可能是驱动真江蓠适应和耐受新的生境的主要分子机制。

关键词：海藻入侵环境适应光合作用转录调控资源再分配

Shifting chemical defence or novel weapons? A review of defence traits in Agarophyton vermiculophyllum and other invasive seaweeds

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Marine Life Science & Technology 2022年第1期4卷 138-149页

作者： Gaoge Wang Yifei Ren Shasha Wang Minglei Hou florian weinberger Institute of Evolution and Marine Biodiversity College of Marine Life SciencesOcean University of ChinaQingdao266003China Marine Ecology Division GEOMAR Helmholtz-Zentrum für Ozeanforschung KielDüsternbrooker Weg 2024105KielGermany

Seaweed bioinvasions increasingly affect coastal environments around the world, which increases the need for predictive models and mitigation strategies. The biotic interactions between seaweed invaders and invaded communities are often considered a key determinant of invasion success and failure and we here revise the current evidence that the capacity of seaweed invaders to deter enemies in newly reached environments correlates with their invasion success. Particularly efficient chemical defences have been described for several of the more problematic seaweed invaders during the last decades. However, confirmed cases in which seaweed invaders confronted un-adapted enemies in newly gained environments with deterrents that were absent from these environments prior to the invasion (so-called “novel weapons”) are scarce, although an increasing number of invasive and non-invasive seaweeds are screened for defence compounds. More evidence exists that seaweeds may adapt defence intensities to changing pressure by biological enemies in newly invaded habitats. However, most of this evidence of shifting defence was gathered with only one particular model seaweed, the Asia-endemic red alga Agarophyton vermiculophyllum, which is particularly accessible for direct comparisons of native and non-native populations in common garden experiments. A. vermiculophyllum interacts with consumers, epibionts and bacterial pathogens and in most of these interactions, non-native populations have rather gained than lost defensive capacity relative to native conspecifics. The increases in the few examined cases were due to an increased production of broad-spectrum deterrents and the relative scarcity of specialized deterrents perhaps reflects the circumstance that seaweed consumers and epibionts are overwhelmingly generalists.

关键词： Agarophyton vermiculophyllum Bioinvasion Chemical defence Novel weapons hypothesis Shifting defence hypothesis

Bayesian inference-based wear prediction method for plain bearings under stationary mixed-friction conditions

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Friction 2024年第6期12卷 1272-1282页

作者： florian KÖNIG florian WIRSING Georg JACOBS Rui HE Zhigang TIAN Ming J.ZUO Institute for Machine Elements and Systems Engineering RWTH Aachen UniversityAachen 52062Germany Department of Mechanical Engineering University of AlbertaEdmonton T6G1H9Canada Qingdao International Academician Park Research Institute Qingdao 266000China

This study introduces a method to predict the remaining useful life(RUL)of plain bearings operating under stationary,wear-critical *** this method,the transient wear data of a coupled elastohydrodynamic lubrication(mixed-EHL)and wear simulation approach is used to parametrize a statistical,linear degradation *** method incorporates Bayesian inference to update the linear degradation model throughout the runtime and thereby consider the transient,system-dependent wear progression within the RUL prediction.A case study is used to show the suitability of the proposed *** results show that the method can be applied to three distinct types of post-wearing-in behavior:wearing-in with subsequent hydrodynamic,stationary wear,and progressive wear *** hydrodynamic operation leads to an infinite lifetime,the method is successfully applied to predict RUL in cases with stationary and progressive wear.

关键词： plain bearings wear modeling remaining useful life prediction Bayesian inference

Methodology for the assessment of the friction torque of ball slewing bearings considering preload scatter

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Friction 2024年第8期12卷 1838-1857页

作者： Iñigo ESCANCIANO Iker HERAS florian SCHLEICH Josu AGUIRREBEITIA Department of Mechanical Engineering University of the Basque CountryBilbao 48080Spain Fraunhofer-Institute for Wind Energy Systems IWES Hamburg 27572Germany

This manuscript presents an innovative methodology for the assessment of the friction torque of ball slewing *** methodology aims to overcome the limitations of state-of-the-art approaches,especially when the friction torque is conditioned by the preload of the *** this end,the authors propose to simulate the preload scatter when solving the load distribution problem,prior to the friction torque *** preload scatter allows to simulate a progressive transition of the balls from a four-point contact state to a two-point contact *** implementing this capability into an analytical model,the authors achieve a successful correlation with experimental ***,depending on the stiffness of the structures to which the bearing is assembled,it is demonstrated that the rigid ring assumption can lead to inaccurate friction torque results when a tilting moment is *** methodology described in this research work is meant to have a practical ***,the manuscript provides guidelines about how to use and tune the analytical model to get a reliable friction torque prediction tool.

关键词： slewing bearing ball bearing friction torque preload scatter

Mechanically Joined Extrusion Profiles for Battery Trays

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Automotive Innovation 2024年第1期7卷 182-193页

作者： florian Kneuper Stefan Neumann AndréSchulze Mortaza Otroshi A.Erman Tekkaya Gerson Meschut Institute of Forming Technology and Lightweight Components(IUL) TU Dortmund University44227 DortmundGermany Laboratory for Material and Joining Technology(LWF) Paderborn UniversityPaderbornGermany

In the context of electromobility,ensuring the leak tightness of assemblies is of paramount importance,particularly in bat-tery *** battery housings,often featuring base assemblies crafted from extruded aluminum profiles,address the challenge of leak tightness at joints through methods like friction stir welding,a process known for its time and cost *** aim of this study is to develop and implement a new type of extruded profile concept to produce tight base assemblies for battery housings by a longitudinal mechanical single stroke joining *** geometry,the process and the properties of the aluminum profiles are investigated to get a joint that meets the tightness requirements and achieve high load-bearing capacities in agreement with the high homologation requirements set to vehicles with high-voltage *** joint is formed by means of a single stage press stroke,which eliminates the need for complex tool designs that are neces-sary for continuous joining(roll joining).Flat steel contact surfaces are used as joining *** evaluate the joint quality,force curves from the joining process are analyzed and the resulting joint geometries are assessed using *** resulting leak tightness of the linear joints is measured by a helium sniffer leak detector and the load-bearing capacities are investigated by shear lap and bending tests and fatigue strength *** study also explores whether a difference in strength between the two joining partners has a positive effect on the joint properties.

关键词： Joining by plastic deformation Aluminum Leak Tightness

Foraging valor linked with aggression:selection against completely abandoning aggression in the high-elevation ant Tetramorium alpestre?

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Insect Science 2024年第3期31卷 953-970页

作者： Marie-Luise Contala Patrick Krapf florian M.Steiner Birgit C.Schlick-Steiner Molecular Ecology Group Department of EcologyUniversitat InnsbruckInnsbruckAustria rganismal&Evolutionary Biology Research Programme University of HelsinkiHelsinkiFinland

Aggression has multiple benefits and is often coupled with other behaviors("behavioral syndromes").The level of aggressiveness is influenced by an adaptive benefit-cost ratio suggesting that benefits should outweigh the costs of ***,we assess if several behaviors are coupled in two behaviorally different populations(aggressive,peaceful)of the high-elevation ant Tetramorium *** three weeks,we collected colony fragments and analyzed boldness,exploring,foraging,and risk-taking *** hypothesized that the aggressive population is bolder,more explorative and risk-prone,and forages more food than the peaceful *** test whether(a)the combination of experiments and parameters used yields a good setup,(b)populations differ behaviorally,and(c)populations display behavioral syndromes,we assessed(a)the frequency of repeatable behaviors of each experiment,(b)the behavioral means among populations,and(c)the behavioral repeatability,*** found that(a)boldness and exploring were most repeatable and represent a good experimental setup,(b)the aggressive population was bolder and more explorative and risk-prone than the peaceful population,(c)boldness and exploring behaviors were highly repeatable in both populations,thus corroborating our *** results suggest that boldness,exploring,and risk-taking but not foraging are presumably coupled with aggression and indicate the presence of behavioral syndromes in this *** specific ecological conditions,aggression may be coupled with other behaviors and important for finding *** is probably adaptive in ***,possibly indicating that selection favors aggression at least partially,which may counteract the complete loss of intraspecific aggression.

关键词： adaptive value behavioral syndromes boldness exploration repeatability trade-off

Outcome prediction after resection of colorectal cancer liver metastases: out with the old, in with the new?

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Hepatobiliary Surgery and Nutrition 2024年第4期13卷 732-735页

作者： Iakovos Amygdalos Daniel Truhn florian W.R.Vondran Department of General VisceralPediatric and Transplantation SurgeryUniversity Hospital RWTH AachenAachenGermany Department of Diagnostic and Interventional Radiology University Hospital RWTH AachenAachenGermany

Colorectal cancer remains one of the commonest and most lethal malignancies worldwide(1).Up to 80%of patients with colorectal cancer develop liver metastases(CRLM),which are the main factor limiting survival(1,2).Despite advances in systemic therapy and the emergence of multimodal treatment concepts,curative surgical resection remains the best therapeutic option and provides the highest chance of long-term survival(1,2).However,liver resection is associated with perioperative morbidity and mortality,while multiple factors affect long-term prognosis(3,4).As a result,various predictive models have been developed over time,to improve preoperative patient selection and foresee unfavourable outcomes.

关键词： Machine learning(ML) artificial intelligence(AI) colorectal liver metastases hepatobiliary

Deep learning-based prediction of 3-dimensional silver contact shapes enabling improved quality control in solar cell metallization

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Energy and AI

Energy and AI 2024年第3期17卷 513-524页

作者： Marius Singler Akshay Patil Linda Ney Andreas Lorenz Sebastian Tepner florian Clement Fraunhofer Institute for Solar Energy Systems(ISE) Heidenhofstr.279110 FreiburgGermany EKRA Automatisierungstechnik GmbH Zeppelinstraße 1674357 BonnigheimGermany

The industrial metallization of Si solar cells predominantly relies on screen printing,with silver as the preferred electrode ***,the design of commercial screens often leads to suboptimal silver usage and increased electrical resistance due to print-related inhomogeneities like mesh marks,constrictions and ***-time monitoring of quality parameters during production has thus become increasingly *** inline optical quality control systems usually only include 2D visualizations of the printed layout,which limits their effectiveness in quality *** that allow 3D measurements are usually slow,expensive,and therefore not worth considering in most *** research focuses on the development of a model that can estimate the three-dimensional shape of printed contact fingers from a single 2D image without the need of additional hardware using deep ***,a workflow for the generation of training data,which involves the creation of image pairs from a 2D microscope and a 3D confocal laser scanning microscope(CLSM)to accurately represent solar cell fingers,is *** model training,the predicted height maps are compared with the ground truth height maps,and the robustness of the model with respect to a paste variation and screen parameter variation is *** results confirm the feasibility and reliability of deep learning-based 3D shape estimation,extending its applicability to new,previously unseen data from screen-printed contact *** a structural similarity index(SSIM)score of 0.76,a strong correlation between the estimated and ground truth height maps is *** summary,our deep learning-based approach for height map estimation offers an effective and reliable solution for fast inline detection and analysis of the cross-sectional area of the printed contact fingers.

关键词： Deep Learning Front-side metallization Image2Heigth Solar cell Screen-printing

Mycorrhizal fungi mitigate nitrogen losses of an experimental grassland by facilitating plant uptake and soil microbial immobilization

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Pedosphere 2024年第2期34卷 399-410页

作者： Yangyang JIA Marcel G.A.VAN DER HEIJDEN Alain Y.VALZANO-HELD Markus JOCHER florian WALDER Postdoctoral Mobile Station of Ecology College of Ecology and EnvironmentXinjiang UniversityÜrümqi 830046China Research Group Plant-Soil Interactions AgroscopeZürich 8046Switzerland Research Group Climate and Agriculture AgroscopeZürich 8046Switzerland Department of Plant and Microbial Biology University of ZürichZürich 8008Switzerland Research Group Soil Quality and Soil Use AgroscopeZürich 8046Switzerland

Nitrogen(N)is one of the most limited nutrients of terrestrial ecosystems,whose losses are prevented in tightly coupled cycles in finely tuned *** change-induced N enrichment through atmospheric deposition and application of vast amounts of fertilizer are now challenging the terrestrial N *** mycorrhizal fungi(AMF)are known drivers of plant-soil nutrient fluxes,but a comprehensive assessment of AMF involvement in N cycling under global change is still ***,we simulated N enrichment by fertilization(low/high)in experimental grassland microcosms under greenhouse conditions in the presence or absence of AMF and continuously monitored different N pathways over nine *** found that high N enrichment by fertilization decreased the relative abundance of legumes and the plant species dominating the plant community changed from grasses to forbs in the presence of AMF,based on aboveground *** presence of AMF always maintained plant N:phosphorus(P)ratios between 14 and 16,no matter how the soil N availability *** in plant N:P ratios due to the increased plant N and P uptake might thus be a primary pathway of AMF altering plant community ***,we constructed a comprehensive picture of AMF’s role in N cycling,highlighting that AMF reduced N losses primarily by mitigating N leaching,while N_(2)O emissions played a marginal *** mycorrhizal fungi reduced N_(2)O emissions directly through the promotion of N_(2)O-consuming *** underlying mechanism for reducing N leaching is mainly the AMF-mediated improved nutrient uptake and AMF-associated microbial *** results indicate that synergies between AMF and other soil microorganisms cannot be ignored in N cycling and that the integral role of AMF in N cycling terrestrial ecosystems can buffer the upcoming global changes.

关键词： N cycling N enrichment N uptake N:P ratio plant community structure symbiotic soil fungi