检索结果-南通市图书馆

Changes of mRNA expression of enkephalin and prodynorphin in hippocampus of rats with chronic immobilization stress

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2004年第17期10卷 2547-2549页

作者： Jia-XuChen weili XinZhao Jian-XinYang Hong-YanXu zhu-FengWang Guang-XinYue SchoolofBasicMedicalScience BeijingUniversityofTraditionalChineseMedicineBeijing100029China

AIM: To observe the changes of enkephalin mRNA and prodynorphin mRNA in hippocampus of rats induced by chronic immobilization ***: Thirty rats were randomly divided into three groups of 10 each: the normal control group (group A),the group induced by chronic immobilization stress for 7 d (group B) and the group induced by chronic immobilization stress for 21 d (group C). The changes of the enkephalin mRNA and prodynorphin mRNA in the rat hippocampus were detected by reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: Expression levels of enkephalin mRNA and prodynorphin mRNA in rat hippocampus were significantly increased under chronic immobilization stress, and the expression of prodynorphin mRNA in the rat hippocampus in group C was remarkably higher than that in group B (0.624±0.026; n = 5; P<0.01).CONCLUSION: The increased enkephalin mRNA and prodynorphin mRNA gene expressions in rat hippocampus were involved in chronic stress.

关键词： mRNA表达脑啡肽海马神经老鼠慢性压力作用基因表达正强啡肽

Reservoir accumulation conditions and key exploration&development technologies for Keshen gas field in Tarim Basin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Petroleum Research 2019年第4期4卷 295-313页

作者： Haijun Yang Yong Li Yangang Tang Ganglin Lei Xiongwei Sun Peng Zhou Lu Zhou Anming Xu Jingjie Tang Wenhui zhu Jiangwei Shang weili Chen Mei Li Exploration and Development Research Institute PetroChina Tarim Oilfield CompanyXinjiang841000China College of Petroleum and Natural Gas Engineering Southwest Petroleum UniversityChengdu610000China

The Keshen gas field is located in the central part of Kuqa foreland thrust belt in Tarim Basin,and is another large gas field discovered in Kuqa depression after Kela 2 gas *** the breakthrough in 2008,a number of large and medium scale gas reservoirs including Keshen 2,Keshen 5 and Keshen 8 have been discovered,that are characterized by ultra depth,ultra-high pressure,ultra-low porosity,ultra-low permeability,high temperature and high *** natural gas geological reserves of nearly trillion cubic meters and production capacity of nearly 5.5 billion cubic meters,the Keshen gas field is the main natural gas producing area in Tarim *** Keshen gas field is located in a series of thrusting imbrication structures in the Kelasu structural belt of Kuqa foreland thrust *** salt roof structure,plastic rheology of salt beds and pre-salt faulted anticlinal structure constitute the large wedge-shaped thrust *** thick delta sandstone of the Cretaceous Bashijike Formation is widely distributed,and it forms the superior reservoir-caprock combination with overlying Paleogene thick gypsum-salt *** deep Jurassic-Triassic oil and gas migrate vertically along fault system formed in Late Himalaya,break through the thick Cretaceous mudstone and move laterally along the fracture system of the pre-salt reservoirs,to form anticline and fault anticline high pressure reservoir *** near ten years of studies,the three-dimensional seismic acquisition and processing technology for complex mountainous areas,extrusion salt-related structural modeling technology and fractured low-porosity sandstone reservoir evaluation technology have been established,which lay a foundation for realization of oil and gas exploration *** acquisition and evaluation technology for high temperature,high pressure,ultra-deep and low-porosity sandstone gas reservoirs,and efficient development technology for fractured tight sandstone gas reservoirs have been

关键词： Oil and gas accumulation Three-dimensional mountainous seismic exploration Ultra-deep high temperature and high pressure gas reservoirs High efficiency exploration and development Keshen gas field Tarim Basin

A systematic survey of LU domain-containing proteins reveals a novel human gene,LY6A,which encodes the candidate ortholog of mouse Ly-6A/Sca-1 and is aberrantly expressed in pituitary tumors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2023年第3期17卷 458-475页

作者： Dan Liu Chunhui Xu Yanting Liu Wen Ouyang Shaojian Lin Aining Xu Yuanliang Zhang Yinyin Xie Qiuhua Huang weili Zhao zhu Chen Lan Wang Saijuan Chen Jinyan Huang Zhe Bao Wu Xiaojian Sun Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Key Laboratory of Systems Biomedicine Ministry of EducationShanghai Center for Systems BiomedicineShanghai Jiao Tong UniversityShanghai 200240China CAS Key Laboratory of Tissue Microenvironment and Tumor Shanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Department of Neurosurgery Center of Pituitary TumorRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Biomedical Big Data Center First Affiliated HospitalZhejiang University School of Medicineand Cancer CenterZhejiang UniversityHangzhou 310000China Department of Neurosurgery First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China

The Ly-6 and uPAR(LU)domain-containing proteins represent a large family of cell-surface *** particular,mouse Ly-6A/Sca-1 is a widely used marker for various stem cells;however,its human ortholog is *** this study,based on a systematic survey and comparative genomic study of mouse and human LU domain-containing proteins,we identified a previously unannotated human gene encoding the candidate ortholog of mouse Ly-6A/*** gene,hereby named LY6A,reversely overlaps with a lncRNA gene in the majority of exonic *** found that LY6A is aberrantly expressed in pituitary tumors,but not in normal pituitary tissues,and may contribute to *** to mouse Ly-6A/Sca-1,human LY6A is also upregulated by interferon,suggesting a conserved transcriptional regulatory mechanism between humans and *** cloned the full-length LY6A cDNA,whose encoded protein sequence,domain architecture,and exon‒intron structures are all well conserved with mouse Ly-6A/*** expression of the LY6A protein in cells demonstrates that it acts the same as mouse Ly-6A/Sca-1 in their processing and glycosylphosphatidylinositol anchoring to the cell ***,these studies unveil a novel human gene encoding a candidate biomarker and provide an interesting model gene for studying gene regulatory and evolutionary mechanisms.

关键词： LU domain-containing protein family novel human gene LY6A pituitary tumor biomarker nonsynonymous SNP GPI-anchored protein

同方期刊数据库

Chinese Society of Clinical Oncology(CSCO) diagnosis and treatment guidelines for malignant lymphoma 2021(English version)

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2021年第3期33卷 289-301页

作者： Jun zhu Jun Ma Union for China Lymphoma Investigators of Chinese Society of Clinical Oncology Zhixiang Shen Zifen Gao Huiqiang Huang Xiaoqiu Li Yexiong Li Lugui Qiu weili Zhao Qingyuan Zhang Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of LymphomaPeking University Cancer Hospital&InstituteBeijing 100142China Department of Hematology&Oncology Harbin Institute of Hematology&OncologyHarbin 150010China 不详 Peking University Health Science Center Sun Yat-sen University Cancer Center Fudan University Shanghai Cancer Center Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Hematology and Blood Diseases Hospital Chinese Academy of Medical Science and Peking Union Medical College Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine Harbin Medical University Cancer Hospital

1. General guidelines2. Diagnosis3. Staging4. Treatment4.1 Diffuse large B-cell lymphoma(DLBCL)4.2 Follicular lymphoma(FL)4.3 Mantle cell lymphoma(MCL)4.4 Marginal zone lymphoma(MZL)4.5 Burkitt lymphoma(BL)4.6 CLL/Small lymphocytic lymphoma(SLL)4.7 Extra-nodal natural killer/T-cell lymphoma(ENKTCL), nasal type4.8 Peripheral T-cell lymphoma(PTCL)4.9 HL4.10 Primary central nervous system lymphoma5. Prognosis Lymphomas are a group of heterogeneous diseases.

关键词： lymphoma diagnosis lymphocytic

Spontaneous running wheel improves neuroprotection efficacy of ischemic postconditioning in mice following ischemia/reperfusion injury

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2018年第3期42卷 79-85页

作者： Hong YE WeiWei Wang Yu Ding XiaoLei Liu WenJI Jia weili Luo HuiJuan Fan HongQun Zhou Jin Wang JianLong Ju DongMing Zhou TianHao Bao YuHong zhu Department of Neurology the Second Affiliated Hospital of Kunming Medical UniversityKunmingChina Department of Cardiology the Second Affiliated Hospital of Kunming Medical UniversityKunmingChina Department of Ultrasound the Second Affiliated Hospital of Kunming Medical UniversityKunmingChina Department of Neurology the First Affiliated Hospital of Kunming Medical UniversityKunmingChina The Mental Health Center of Kunming Medical University KunmingChina Kunming Medical University KunmingChina Biomedical Engineering University of AlbertaEdmontonAlbertaCanada

Ischemic postconditioning(IP)has been shown to provide protection for ischemia/reperfusion(IR)injury,but its efficacy is *** this study we hypothesized that spontaneous running wheel(RW)could improve neuroprotection efficacy of IP for *** established mouse models of IR and showed that compared to Sham group,IR group had obvious brain infract and neurological *** IR+IP group,brain infract and neurological dysfunction improved compared to IR ***,in IR+IP+RW group brain infract and neurological dysfunction improved much *** assay showed that IP but not RW significantly reduced the number of apoptotic cells after ***,the number of apoptotic cells was significantly reduced in RW+IP *** addition,the levels of pro-apoptotic factors increased in IR group but significantly reduced in IR+IP+RW group,while the levels of antiapoptotic factors decreased in IR group but significantly increased in IR+IP+RW ***,in IR+IP+RW group,MDA level was further decreased and SOD level was further increased compared to IR+IP ***,both PI3K inhibitor and STAT3 inhibitor significantly worsened brain infract and neurological dysfunction and promoted apoptosis in IR *** conclusion,RW combined with IP reduces brain infract and neurological dysfunction in mice after IR,and this is associated with enhanced anti-apoptotic and anti-oxidant benefits via the activation of PI3K and STAT3 pathways.

关键词： Ischemic postconditioning ischemia/reperfusion running wheel apoptosis PI3K STAT3

532nm HfO_2/SiO_2高反膜的激光预处理效应

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

强激光与粒子束 2015年第3期27卷 254-259页

作者：刘杰张伟丽朱美萍中国科学院上海光学精密机械研究所上海201800 中国科学院大学北京100049

采用单台阶能量光栅扫描以及R-on-1测试两种不同预处理方式研究了激光预处理技术对532nm HfO2/SiO2高反膜的阈值提升效果。用Nd:YAG二倍频激光对电子束蒸发制备的532nm HfO2/SiO2高反膜进行1-on-1损伤阈值测试,然后分别进行单台阶能量... 详细信息

采用单台阶能量光栅扫描以及R-on-1测试两种不同预处理方式研究了激光预处理技术对532nm HfO2/SiO2高反膜的阈值提升效果。用Nd:YAG二倍频激光对电子束蒸发制备的532nm HfO2/SiO2高反膜进行1-on-1损伤阈值测试,然后分别进行单台阶能量光栅扫描以及R-on-1测试。通过对损伤概率以及损伤形貌的分析,发现激光预处理能够去除薄膜内低阈值缺陷,达到提高损伤阈值的目的,损伤阈值分别提高38%和30%。

关键词：电子束蒸发 532nm高反膜激光预处理损伤阈值缺陷

UPLC-Q-TOF-MS联合网络药理学探讨当归饮子对特应性皮炎小鼠的作用机制

在线全文

学校读者我要写书评

暂无评论

中国畜牧兽医 2025年第2期52卷 874-889页

作者：黄永熙闫普普郭伟丽李亚娜朱君刘满侯超群覃美林李蓉武毅苏应兵杨小林郭利伟王雄戴刚长江大学动物科学技术学院湿地生态与农业利用教育部工程研究中心荆州434000 河北省沙河市第六中学沙河054100 宜昌人福药业有限责任公司宜昌443000 云南农业大学动物医学院昆明650500 荆州市明德科技有限公司荆州434001

[目的]探讨当归饮子(Angelica decoction, DGYZ)治疗2,4-二硝基氯苯(DNCB)诱导的小鼠特应性皮炎(atopic dermatitis, AD)皮肤病变效果,为临床宠物AD的诊断和治疗提供参考。[方法]选用90只BALB/c小鼠,分为6组:空白组(Control)、DNCB组、DGYZ低(9.8 mg/kg DGYZ)、中(19.6 mg/kg DGYZ)、高(39.3 mg/kg DGYZ)剂量组和西替利嗪组(4 mg/kg西替利嗪),每组15只小鼠。空白组小鼠涂抹丙酮溶液作为对照,其余各组小鼠通过背部反复涂抹DNCB建立小鼠AD样皮肤病模型,并按分组进行药物治疗。治疗结束后采集小鼠背部皮肤、血液及脾脏用于后续分析。通过HE和甲苯胺蓝染色观察小鼠皮肤组织厚度和肥大细胞数;ELISA方法检测小鼠血清中免疫球蛋白E(IgE)水平;全自动血细胞分析仪测定血液中白细胞(WBC)、中性粒细胞和嗜酸性粒细胞的含量变化;流式细胞术检测小鼠脾脏CD4^(+)/CD8^(+)和Th1/2细胞分化;实时荧光定量PCR测定小鼠背部皮肤中细胞因子相关mRNA的表达水平;超高压液相色谱-飞行时间质谱仪(UPLC-TOF-MS)分析DGYZ的主要活性成分,联合网络药理学探讨DGYZ治疗AD的主要靶点和作用机制。最后采用实时荧光定量PCR检测JAK1-STAT3信号通路的mRNA表达水平。[结果]与空白组相比,DNCB组小鼠背部皮肤出现严重的皮肤病变和表皮增厚现象,血液中白细胞、中性粒细胞和嗜酸性粒细胞数目显著上升(P<0.05);与DNCB组相比,DGYZ各剂量组和西替利嗪组小鼠表皮增厚现象均得到有效缓解,血液中白细胞、中性粒细胞和嗜酸性粒细胞数目均下降,且DGYZ高剂量组及西替利嗪组差异显著(P<0.05);DGYZ高剂量组和西替利嗪组小鼠血清中IgE表达水平显著降低(P<0.05)。流式细胞术结果显示,DNCB组小鼠CD4^(+)/CD8^(+)和Th1/2细胞的平衡受到干扰,不同剂量DGYZ均可显著改善这一情况(P<0.05)。实时荧光定量PCR检测结果显示,与空白组相比,DNCB组小鼠皮肤组织中γ-干扰素(IFN-γ)和白细胞介素-12(IL-12)基因mRNA表达水平显著下调(P<0.05);IL-4、IL-13、IL-6基因mRNA表达水平显著上调(P<0.05)。与DNCB组相比,饲喂DGYZ治疗后皮肤组织中IFN-γ和IL-12基因mRNA表达水平逐渐上调,IL-4、IL-13、IL-6基因mRNA表达水平显著下调(P<0.05)。UPLC-TOF-MS鉴定出89种DGYZ成分,其中活性成分64种。网络药理学分析结果表明,以上64种活性成分通过作用于信号传导及转录激活蛋白3(STAT3)、蛋白激酶B(AKT1)等基因及JAK/STAT、NF-κB和Th17细胞分化信号通路,对AD样皮肤病变发挥治疗作用。实时荧光定量PCR检测结果表明,DNCB可诱导小鼠JAK1-STAT3信号通路的激活,DGYZ可抑制该途径的激活。[结论]DGYZ可减少小鼠皮肤中肥大细胞数目和血清中IgE的表达,调节免疫平衡,降低促炎因子的mRNA表达并抑制JAK1-STAT3信号通路激活,缓解AD样皮肤病变。本研究结果可为中药治疗动物AD样皮肤病变提供依据。

关键词：当归饮子(DGYZ) AD样皮肤病变 2,4-二硝基氯苯(DNCB) 流式细胞术网络药理学

维普期刊数据库博看期刊

同方期刊数据库

在线全文

学校读者我要写书评

暂无评论

Rapid Rail

ChinAfrica 2011年第9期3卷 16-17页

作者： Hou weili

Beijing to Shanghai train trip halved to five hours HEARING the news that the high-speed rail (HSR) train from Beijing to Shanghai began to operate on June 30,2011,zhu Lijun,who works in the Beijing branch of a Shangh... 详细信息

关键词： HSR Rapid Rail

Real-time contrast enhanced ultrasound imaging of focal splenic lesions

在线全文

学校读者我要写书评

暂无评论

Real-time contrast enhanced ultrasound imaging of focal sple...