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Functional assessment of cadherin as a shared mechanism for cross/dual resistance to Cry1Ac and Cry2Ab in Helicoverpa zea

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Journal of Integrative Agriculture 2024年第5期23卷 1604-1617页

作者： Jizhen wei Min Zhang Pin Li Zhongyuan Deng Xinming Yin Shiheng An Xianchun Li State Key Laboratory of Wheat and Maize Crop Science/College of Plant Protection Henan Agricultural UniversityZhengzhou 450046China Department of Entomology and BIO5 Institute University of ArizonaTucsonAZ 85721USA School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China

Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United ***-selected Cry1Ac/Cry2Ab cross resistance and fieldevolved practical dual resistance of *** to these two toxins have been widely *** the widespread Cry1Ac/cy2Ab dual resistance of *** has resulted from the selection of one shared or two independent resistance mechanisms by pyramided Bt crops remains *** is a well-confirmed receptor of Cry1Ac and a suggested receptor of Cry2Ab in at least three Lepidopteran *** test whether cadherin may serve as one shared mechanism for the cross and dual resistance of *** to Cry1Ac and Cry2Ab,we cloned *** cadherin(HzCadherin)cDNA and studied its functional roles in the mode of action of Cry1Ac and Cry2Ab by gain-and lossof-function *** expression of HzCadherin in *** midgut,*** fat body and Sf9 cells made all three of these cell lines more susceptible to activated Cry1Ac but not activated Cry2Ab,whereas silencing HzCadherin of *** midgut and fat body cells significantly reduced the susceptibility to Cry1Ac but not ***,suppressing HzCadherin with siRNA made *** larvae resistant to *** results clearly demonstrate that HzCadherin is not a receptor for Cry2Ab,and thus it is unlikely to serve as one shared mechanism for the cross and dual resistance of *** to Cry1Ac and Cry2Ab.

关键词： Bt crops cadherin Cry toxin receptor resistance mechanism

Game Theory based Optimal Defensive Resources Allocation with Incomplete Information in cyber-physical Power Systems against False Data Injection Attacks

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Protection and Control of Modern Power Systems 2024年第2期9卷 115-127页

作者： Bingjing Yan Zhenze Jiang Pengchao Yao Qiang Yang wei Li Albert Y.Zomaya College of Electrical Engineering Zhejiang University College of Control Science and Engineering Zhejiang University College of Electrical Engineering Zhejiang UniversityHangzhou 310027China School of Information Technologies at The University of Sydney School of Information Technologies

Modern power grid is fast emerging as a complex cyber-physical power system(CPPS)integrating physical current-carrying components and processes with cyber-embedded computing,which faces increasing cy-berspace security threats and *** this paper,the state(i.e.,voltage)offsets resulting from false data injection(FDI)attacks and the bus safety characterization are applied to quantify the attack *** state offsets are obtained by the state estimation method,and the bus safety characterization considers the power net-work topology as well as the vulnerability and connection relationship of *** the indeterminacy of attacker’s resource consumption and reward,a zero-sum game-theoretical model from the defender’s perspective with incomplete information is explored for the optimal allocation of limited defensive *** attacker aims to falsify measurements without triggering threshold alarms to break through the protection,leading to load shedding,over-voltage or *** defender attempts to ensure the estimation results to be as close to the actual states as possible,and guarantee the system’s safety and efficient defensive resource *** proposed solution is extensively evaluated through simu-lations using the IEEE 33-bus test network and real-time digital simulator(RTDS)based testbed experiments of the IEEE 14-bus *** results demonstrate the effec-tiveness of the proposed game-theoretical approach for optimal defensive resource allocation in CPPS when lim-ited resources are available when under FDI *** Terms—Optimal strategy,game theory,Nash equilibrium,CPPS,FDI attack.

关键词： Optimal strategy game theory Nash equilibrium CPPS FDI attack

A human-specific insertion promotes cell proliferation and migration by enhancing TBC1D8B expression

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Science China(Life Sciences) 2024年第4期67卷 765-777页

作者： Hui Zhao Lin-Lin Liu Jian Sun Lian Jin Hai-Bing Xie Jian-Bo Li Hui Xu Dong-Dong Wu Xiao-Lin Zhuang Min-Sheng Peng Ya-Jun Guo wei-Zhu Qian Newton OOtecko wei-Jie Sun Liang-Hu Qu Jie He Zhao-Li Chen Rong Liu Ce-Shi Chen Ya-Ping Zhang State Key Laboratory for Conservation and Utilization of Bio-resource School of Life SciencesSchool of Ecology and Environmental ScienceYunnan UniversityKunming 650091China School of Forensic Medicine Kunming Medical UniversityKunming 650500China Academy of Biomedical Engineering Kunming Medical UniversityKunming 650500China The Third Affiliated Hospital Kunming Medical UniversityKunming 650118China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming 650201China State Key Laboratory of Genetic Resources and Evolution Yunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunming 650201China The Key Laboratory of Gene Engineering of the Ministry of Education State Key Laboratory for BiocontrolSun Yat-sen UniversityGuangzhou 510275China National Engineering Research Center for Antibody Medicine and Shanghai Key Laboratory of Cell Engineering and Antibody Shanghai 201203China Department of Thoracic Surgery Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Human-specific insertions play important roles in human phenotypes and *** we reported a 446-bp insertion(Insert-446)in intron 11 of the TBC1D8B gene,located on chromosome X,and traced its origin to a portion of intron 6 of the EBF1 gene on chromosome ***,Insert-446 was present in the human Neanderthal and Denisovans genomes,and was fixed in humans after human-chimpanzee *** have demonstrated that Insert-446 acts as an enhancer through binding transcript factors that promotes a higher expression of human TBC1D8B gene as compared with orthologs in *** addition,over-expression TBC1D8B promoted cell proliferation and migration through“a dual finger”catalytic mechanism(Arg538 and Gln573)in the TBC domain in vitro and knockdown of TBC1D8B attenuated tumorigenesis in *** of Insert-446 prevented cell proliferation and migration in cancer and normal *** results reveal that the human-specific Insert-446 promotes cell proliferation and migration by upregulating the expression of TBC1D8B *** findings provide a significant insight into the effects of human-specific insertions on evolution.

关键词： human-specific insertion enhancer gene expression cell proliferation and migration

Highly efficient discovery of the covalent Minhibitors from crude Pu-erh tea by integrating biochemical and chemoproteomic approaches

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Food Science and Human Wellness 2024年

作者： Guang-Hao Zhu Ya-Ni Zhang Sheng-Lan Qi Jian-Rong Xu Yuan Xiong Zhao-Qin Zhang Xi-Xiang Chen Yuan-Yuan Xie Xiao-Qing Guan wei-Dong Zhang wei Liu Guang-Bo Ge Shanghai Frontiers Science Center of TCM Chemical Biology Institute of Interdisciplinary Integrative Medicine Research Shanghai University of Traditional Chinese Medicine Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

The main proteases (Mpros) are hydrolases playing essential roles in the replication of β-coronaviruses including SARS-CoV-2. Herein， a highly efficient strategy was developed for discovering the Mproinactivators from crude plant extract integrating target-based biochemical assay and chemoproteomic approaches. Firstly， Pu-erh tea was found to potently suppress SARS-CoV-2 Mproin a time-dependent manner. Next， global chemical analysis coupling with peptide-modification profiling were used to identify the cysteine-modified constituents in Pu-erh tea. The results suggested that seven constituents in Pu-erh tea could modify SARS-CoV-2 Mpro， which turned out that epigallocatechin， gallocatechin and gallic acid were the most efficacious Mproinactivators. Further investigations demonstrated that epigallocatechin and gallocatechin could inactivate SARS-CoV-2 Mprovia blocking the formation of the homodimers. Collectively， this work proposed a novel and practical strategy for highly efficient discovery of time-dependent inhibitors of SARS-CoV-2 Mprofrom plant extracts， while three constituents in Pu-erh tea have emerged as robust SARS-CoV-2 Mproinactivators.

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改进MFO-LSTM网络的风电机组齿轮箱故障预警研究

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机床与液压 2024年第4期52卷 185-194页

作者：周伟魏鑫李西兴湖北工业大学机械工程学院湖北武汉430068

风电机组齿轮箱在数据采集与监控系统(SCADA)的帮助下,通过监控齿轮箱油温是否超过阈值实现故障报警,其判断精度不高且问题发现不及时,因此使用长短期记忆网络模型(LSTM)融合SCADA数据实现对齿轮箱油温状态的预测。用齿轮箱正常运行状... 详细信息

风电机组齿轮箱在数据采集与监控系统(SCADA)的帮助下,通过监控齿轮箱油温是否超过阈值实现故障报警,其判断精度不高且问题发现不及时,因此使用长短期记忆网络模型(LSTM)融合SCADA数据实现对齿轮箱油温状态的预测。用齿轮箱正常运行状态下的数据训练LSTM模型,计算油温预测值与真实值之间的残差,根据正态分布的原则设置残差的上下预警阈值,用来对齿轮箱故障进行预警。为简化训练模型的复杂度,在SCADA数据中选用与齿轮箱油温相关性较为密切的参数作为LSTM模型的输入项。为降低因LSTM模型超参数设置不当造成的预测准确度表现不佳,提出改进飞蛾火焰算法(MFO)与LSTM的组合模型,在保留MFO算法强大的全局搜索能力的同时,使其避免陷入局部搜索的陷阱,通过改进MFO对LSTM模型参数进行迭代优化,最终构建合适的模型。最后通过某风电机组SCADA数据验证该方法能够有效预警齿轮箱的故障,并且与其他方法相比准确度更高,预警更及时,迭代效果更好。

关键词：风电机组齿轮箱长短期记忆网络模型(LSTM) 故障预警数据采集与监控系统(SCADA) 飞蛾火焰算法(MFO)

氧化苦参碱联合拉米夫定治疗慢性乙型肝炎疗效观察

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新乡医学院学报 2003年第1期20卷 41-42页

作者：刘朝阳魏素云于培龙安阳市第五人民医院河南安阳455000

目的　观察氧化苦参碱注射液联合拉米夫定治疗慢性乙型肝炎 (CHB)的临床疗效。方法　 3 1例HBVDNA及HBeAg阳性的CHB接受氧化苦参碱注射液联合拉米夫定治疗12个月 ,并与 3 5例仅接受拉米夫定治疗的患者进行对比。观察两组的谷丙转氨酶、H... 详细信息

目的　观察氧化苦参碱注射液联合拉米夫定治疗慢性乙型肝炎 (CHB)的临床疗效。方法　 3 1例HBVDNA及HBeAg阳性的CHB接受氧化苦参碱注射液联合拉米夫定治疗12个月 ,并与 3 5例仅接受拉米夫定治疗的患者进行对比。观察两组的谷丙转氨酶、HBVDNA、HBeAg的变化情况及不良反应。结果　治疗 1个月治疗组与对照组的ALT复常率分别为 87.1% (2 7/ 3 1)和 85 .7% (3 0 / 3 5 ) ,治疗 4个月ALT均恢复正常。治疗结束时观察组HBVDNA转阴率 87.1% (2 7/ 3 1) ,与对照组 82 .9% (2 9/ 3 5 )无明显差异 (P >0 .0 5 ) ,观察组HBeAg转阴率为 61.3 % (19/ 3 1) ,高于对照组 3 7.1% (13 / 3 5 ) (P <0 .0 5 )。治疗结束 1年后观察组HBVDNA和HBeAg有 5 6.5 % (13 / 2 3 )表现为持续阴性 ,明显较对照组 2 0 .0 % (5 / 2 5 )高(P <0 .0 5 )。治疗组HBVDNA和HBeAg转阴后的复发率也分别低于对照组 (P <0 .0 5 )。结论　氧化苦参碱注射液与拉米夫定联用有协同作用 ,可有效降低复发率。

关键词：疗效氧化苦参碱拉米夫定慢性乙型肝炎

基于实测数据的半潜式平台贝叶斯模型修正方法研究

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船舶力学 2024年第2期28卷 179-190页

作者：李松武文华姚伟岸大连理工大学工业装备结构分析优化与CAE软件全国重点实验室辽宁大连116024 大连理工大学宁波研究院浙江宁波315000

随着半潜式平台的长期海上服役,平台的基本设计参数会发生变化,如仍基于原始设计参数对平台进行分析将影响结果的准确性。本文以半潜式平台服役中易发生变化的基本参数(平台排水量、重心高度和三个自由度回转半径)作为修正参数,以平台... 详细信息

随着半潜式平台的长期海上服役,平台的基本设计参数会发生变化,如仍基于原始设计参数对平台进行分析将影响结果的准确性。本文以半潜式平台服役中易发生变化的基本参数(平台排水量、重心高度和三个自由度回转半径)作为修正参数,以平台横摇、纵摇和垂荡自由度的方差和波频功率谱密度构建目标函数,建立可依据实测数据的半潜式平台贝叶斯参数修正方法。以我国某半潜式平台实测数据为例,开展平台的参数修正研究,并基于修正后的参数对平台多年一遇环境荷载下的响应进行对比评估。

关键词：半潜式平台原型监测贝叶斯参数修正代理模型

基于横摇衰减数据的横摇阻尼估算方法对比研究

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船舶力学 2024年第3期28卷 328-340页

作者：章东王文涛卜淑霞刘伟中国船舶科学研究中心江苏无锡214082 水动力学重点实验室江苏无锡214082

基于自由横摇衰减曲线估算船舶横摇阻尼是一种常用工程手段,由于衰减曲线估算阻尼的方法众多,导致阻尼估算结果存在差异。为实现获取高精度阻尼预报方法的目的,本文首先对近年来出现的基于衰减曲线估算横摇阻尼的方法原理进行详细分析,... 详细信息

基于自由横摇衰减曲线估算船舶横摇阻尼是一种常用工程手段,由于衰减曲线估算阻尼的方法众多,导致阻尼估算结果存在差异。为实现获取高精度阻尼预报方法的目的,本文首先对近年来出现的基于衰减曲线估算横摇阻尼的方法原理进行详细分析,扩展其适用范围,按照原理将方法分为基于分段线性假设与基于参数识别两类。其次利用标准模型(DTC)的横摇数据,验证并比较不同方法的特点,分析阻尼系数估算的误差来源。结果表明,横摇阻尼估算精度与使用的方法强相关,推荐物理意义明确、误差来源少、对测量噪声与误差不敏感的Froude能量法作为初步阻尼评估方法,若能找到足够精确的阻尼模型,则可进一步使用具有内在的噪声抑制机制、不需要预估阻尼系数初值、存在唯一解的Prony-SS方法以提高识别精度。

关键词：横摇阻尼估算横摇衰减曲线消灭曲线法阻尼模型

The OsWRKY72-OsAAT30/OsGSTU26 module mediates reactive oxygen species scavenging to drive heterosis for salt tolerance in hybrid rice

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Journal of Integrative Plant Biology 2024年第4期66卷 709-730页

作者： Citao Liu Bigang Mao Yanxia Zhang Lei tian Biao Ma Zhuo Chen Zhongwei wei Aifu Li Ye Shao Gongye Cheng Lingling Li Wenyu Li Di Zhang Xiaoping Ding Jiangxiang Peng Yulin Peng Jiwai He Nenghui Ye Dingyang Yuan Chengcai Chu Meijuan Duan Hunan Provincial Key Laboratory of Stress Biology College of AgricultureHunan Agricultural UniversityChangsha 410128China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125China College of Agriculture South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Wheat and Maize Crop Science Center for Crop Genome EngineeringCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMI 48109 USA Biobin Data Science Co. Ltd.Changsha 410221China

Hybrid rice(Oryza sativa)generally outperforms its inbred parents in yield and stress tolerance,a phenomenon termed heterosis,but the underlying mechanism is not completely ***,we combined transcriptome,proteome,physiological,and heterosis analyses to examine the salt response of super hybrid rice Chaoyou1000(cy1000).In addition to surpassing the mean values for its two parents(mid-parent heterosis),cy1000 exhibited a higher reactive oxygen species scavenging ability than both its parents(over-parent heterosis or heterobeltiosis).Nonadditive expression and allele-specific gene expression assays showed that the glutathione S-transferase gene OsGSTU26 and the amino acid transporter gene OsAAT30 may have major roles in heterosis for salt tolerance,acting in an overdominant fashion in ***,we identified OsWRKY72 as a common transcription factor that binds and regulates OsGSTU26 and *** salt-sensitive phenotypes were associated with the OsWRKY72^(paternal)genotype or the OsAAT30^(maternal)genotype in core rice germplasm ***72^(paternal)specifically repressed the expression of OsGSTU26 under salt stress,leading to salinity sensitivity,while OsWRKY72^(maternal)specifically repressed OsAAT30,resulting in salinity *** results suggest that the OsWRKY72-OsAAT30/OsGSTU26 module may play an important role in heterosis for salt tolerance in an overdominant fashion in cy1000 hybrid rice,providing valuable clues to elucidate the mechanism of heterosis for salinity tolerance in hybrid rice.

关键词： allele-specific gene expression(ASE) heterosis hybrid rice nonadditive effect(NAE) salt tolerance transcriptome and proteome