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Construction of a telomerase-immortalized porcine tracheal epithelial cell model for swine-origin mycoplasma infection

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Journal of Integrative Agriculture 2022年第2期21卷 504-520页

作者： XIE Xing HAO Fei WANG Hai-yan PANG Mao-da GAN Yuan LIU Bei-bei ZHANG Lei wei yan-na CHEN Rong ZHANG Zhen-zhen BAO Wen-bin BAI Yun SHAO Guo-qing XIONG Qi-yan FENG Zhi-xin Key Laboratory for Veterinary Bio-Product Engineering of Ministry of Agriculture and Rural Affairs Institute of Veterinary MedicineJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology Institute of Food Safety and NutritionJiangsu Academy of Agricultural SciencesNanjing 210014P.R.China College of Animal Science and Technology Yangzhou UniversityYangzhou 225009P.R.China

Primary porcine tracheal epithelial cells(PTECs) are an appropriate model for studying the molecular mechanism of various porcine respiratory diseases, including swine-origin mycoplasmas, which are isolated from respiratory tract of pigs and mainly found on the mucosal surface surrounding swine trachea. However, the short proliferation ability of primary PTECs greatly limits their lifespan. In this study, primary PTECs were carefully isolated and cultured, and immortal PTECs were constructed by transfecting primary PTECs with the recombinant constructed plasmid pEGFPhTERT containing human telomerase reverse transcriptase(hTERT). Immortal PTECs(hTERT-PTECs) maintained both the morphological and functional characteristics of primary PTECs, as indicated by the expression of cytokeratin 18, cellcycle analysis, proliferation assay, Western blotting, telomerase activity assay, karyotype analysis and quantitative RTPCR. Compared to primary PTECs, hTERT-PTECs had an extended replicative lifespan, higher telomerase activity, and enhanced proliferative activity. In addition, this cell line resulted in a lack of transformed and grown tumors in nude mice, suggesting that it could be safely applied in further studies. Moreover, hTERT-PTECs were vulnerable to all swineorigin mycoplasmas through quantitative analysis as indicated by 50% color changing unit(CCU_(50)) calculation, and no significant differences of adhesion ability between primary and immortal PTECs were observed. For the representative swine mycoplasma Mycoplasma hyopneumoniae(Mhp), except for Dna copies quantitative real-time PCR assay, indirect immunofluorescence assay and Western blotting analysis also depicted that hTERT-PTECs was able to adhere to different Mhp strains of different virulence. In summary, like primary PTECs, hTERT-PTECs could be widely used as an adhesion cell model for swine-origin mycoplasmas and in infection studies of various porcine respiratory pathogens.

关键词： porcine tracheal epithelial cells(PTECs) hTERT-PTECs swine-origin mycoplasmas adhesion cell model

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Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

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nano-Micro Letters 2025年第1期17卷 281-292页

作者： Xing Wang wei Pi Sheng Hu Haifeng Bao na Yao wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

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Solar cycles during the seventeenth century revealed by equatorial aurora records

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Earth and Planetary Physics 2025年第1期9卷 182-187页

作者： Yong wei LiMei yan Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

Solar cycles are fundamental to astrophysics,space exploration,technological infrastructure,and Earth's climate.A better understanding of these cycles and their history can aid in risk mitigation on Earth,while also deepening our knowledge of stellar physics and solar system *** the solar cycles between 1600 and 1700-especially the post-1645 Maunder Minimum,characterized by significantly reduced solar activity-poses challenges to existing solar activity *** study utilizes a new red equatorial auroral catalog from ancient Korean texts to establish solar cycle patterns from 1623 to ***,a further reevaluation of the solar cycles between 1610 and 1755 identified a total of 13 cycles,diverging from the widely accepted record of 12 cycles during that *** research enhances our understanding of historical solar activity,and underscores the importance of integrating diverse historical sources into modern analyses.

关键词： solar cycle Maunder Minimum solar activity red equatorial aurora West Pacific geomagnetic anomaly

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新媒体纪录片的存在形态与审美取向

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艺术百家 2015年第4期31卷 226-228页

作者：闫伟娜济南大学历史与文化产业学院山东济南250022

在纪录片近百年的发展过程中,其传播媒介历经电影、电视再到网络的不断演化,催生出不同形态的纪录片类型,新媒体纪录片就是其中的一种。当前新媒体纪录片的存在形态主要包括网站自制纪录片、商业定制微纪录片和用户原创纪录片UGC。新媒... 详细信息

在纪录片近百年的发展过程中,其传播媒介历经电影、电视再到网络的不断演化,催生出不同形态的纪录片类型,新媒体纪录片就是其中的一种。当前新媒体纪录片的存在形态主要包括网站自制纪录片、商业定制微纪录片和用户原创纪录片UGC。新媒体纪录片在美学维度上呈现出两极化的特点:一方面是作为一种文化商品话语的商业性与精英性,另一方面是作为一种底层话语叙述的人文性与草根性,这两方面的审美维度在新媒体的发展语境下又呈现出趋近性,表现为个体创作与大众文化消费之间的链接。

关键词：电影艺术新媒体纪录片存在形态审美取向网站自制纪录片商业定制微纪录片用户原创纪录片

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吩噻嗪染料亚甲基蓝共振瑞利散射法测利福平

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化学研究与应用 2013年第5期25卷 751-753页

作者：江虹韦燕娜长江师范学院化学化工学院重庆408100

建立了测定痕量利福平的共振瑞利散射(RRS)法。在naOH溶液中,利福平与亚甲基蓝相互作用后,共振瑞利散射显著增强,在371 nm处的ΔIRRS最强。利福平的质量浓度在0.20～1.65 mg.L-1范围内与△IRRS成正比,检出限(3Sb/S)为0.038 mg.L-1。该... 详细信息

建立了测定痕量利福平的共振瑞利散射(RRS)法。在naOH溶液中,利福平与亚甲基蓝相互作用后,共振瑞利散射显著增强,在371 nm处的ΔIRRS最强。利福平的质量浓度在0.20～1.65 mg.L-1范围内与△IRRS成正比,检出限(3Sb/S)为0.038 mg.L-1。该法可用于人体血液及市售利福平药物中利福平含量的测定,结果满意。

关键词：吩噻嗪染料亚甲基蓝共振瑞利散射利福平

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基于蒙特卡洛模拟的商业银行排队问题研究

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技术经济与管理研究 2009年第1期 20-22页

作者：颜薇娜浙江大学管理学院浙江杭州310058

本文以排队论为理论基础,用蒙特卡洛模拟在Excel上对银行柜台多服务器单队列(M/M/C)的服务状况进行了动态模拟。得到不同顾客到达速率下,银行排队状况和银行为满足一定服务水平应该开设的服务器个数,并对目前日益严重的银行排队问题提... 详细信息

本文以排队论为理论基础,用蒙特卡洛模拟在Excel上对银行柜台多服务器单队列(M/M/C)的服务状况进行了动态模拟。得到不同顾客到达速率下,银行排队状况和银行为满足一定服务水平应该开设的服务器个数,并对目前日益严重的银行排队问题提出了建议。

关键词：排队论动态模拟蒙特卡洛模拟

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基于遗传算法的VQ码本设计及语音识别

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计算机工程与应用 2007年第17期43卷 71-73页

作者：王社国魏艳娜河北工程大学信息与电气工程学院河北邯郸056038

在矢量量化(VQ)的码本设计过程中,经典的LBG算法收敛速度快,但极易陷入局部最优,且初始码本的生成对最佳码本的设计影响很大。考虑到遗传算法(GA)是一种具有全局优化搜索能力的算法,提出了GA和LBG算法相结合的GA-L算法来优化码本,改善... 详细信息

在矢量量化(VQ)的码本设计过程中,经典的LBG算法收敛速度快,但极易陷入局部最优,且初始码本的生成对最佳码本的设计影响很大。考虑到遗传算法(GA)是一种具有全局优化搜索能力的算法,提出了GA和LBG算法相结合的GA-L算法来优化码本,改善了码本质量,并将其应用于汉语连续数字语音识别中,实验结果表明了GA-L算法的有效性。

关键词：遗传算法矢量量化 GA—L 语音识别

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Discovery of a new family member of arsenic–vanadium polyoxometalates with high catalytic performance for one-pot coupling of CO2, epoxides and amines

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Tungsten 2025年第1期7卷 80-87页

作者： Fang-Qian Wang Yin Zhang wei-wei Wang Mei-Qian Fu Hong-Bing Mo wei-Dong Yu Chao Liu Jun yan College of Chemistry and Chemical Engineering Central South University Junior Education Department Changsha Normal University School of Science Hunan University of Technology and Business

Arsenic-vanadium polyoxometalates（POMs） represent a family of tremendous potential catalysts owing to their peculiar physical and chemical *** in CO2conversion,using POMs to catalyze the conversion of CO2and epoxide into chemical intermediates is the research ***,A new family number of arsenic-vanadium POMs,[N（CH3）4]4[As8V12O38（H2O）]·2H2O({As8V12O38}),was reported and characterized by single-crystal X-ray diffraction,fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,*** from other allotropicity,{As8V12O38}shows a different structure pattern with an I-43d *** obtained {As8V12O38} was employed as a catalyst for the *** initial catalytic reaction confirmed that {As8V12O38} can catalyze the formation of epoxy carbonate by using 1.01 kPa CO2and epoxide at 70℃.Furtherly,when added amine in the catalytic system,oxazolidinone could be obtained by high yields,which provides an efficient way to convert CO2into profitable ***,a possible catalytic reaction mechanism was proposed by using different characterization analyses.{As8V12O38} first catalyzes the epoxide and CO2to form an epoxy carbonate and then catalyzes epoxy carbonate and benzylamine into oxazolidinone.

关键词： Arsenic CO2 conversion Oxazolidinone Polyoxometalates Vanadium

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Morphology engineering of ZnO micro/nanostructures under mild conditions for optoelectronic application

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 498-503页

作者： Liang Chu Haoyu Shen Hudie wei Hongyu Chen Guoqiang Ma Wensheng yan Institute of Carbon Neutrality and New Energy&School of Electronics and Information Hangzhou Dianzi UniversityHangzhou 310018China School of Applied Physics and Materials Wuyi UniversityJiangmen 529020China

Zinc oxide(ZnO)serves as a crucial functional semiconductor with a wide direct bandgap of approximately 3.37 *** reaction is commonly used in the synthesis of ZnO micro/nanostructures,given its low cost,simplicity,and easy ***,ZnO morphology engineering has become desirable through the alteration of minor conditions in the reaction process,particularly at room *** this work,ZnO micro/nanostructures were synthesized in a solution by varying the amounts of the ammonia added at low temperatures(including room temperature).The formation of Zn^(2+)complexes by ammonia in the precursor regulated the reaction rate of the morphology engineering of ZnO,which resulted in various structures,such as nanoparticles,nanosheets,microflowers,and single ***,the obtained ZnO was used in the optoelectronic application of ultraviolet detectors.

关键词： morphology engineering low temperature ZnO nanosheets microflowers ultraviolet detector

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Experimental Study for Effect of Multi-Site Spark Ignition on Dedicated Hybrid Engine Performance under High Dilution Condition

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Journal of Thermal Science 2025年第1期34卷 254-267页

作者： yan Bowen WATERS Benjamin HAINES Andrew MCGHEE Mike HU Tiegang DENG wei PU Yongxian MA Tianyu Powertrain Engineering R&D Center Chongqing Changan Automobile Co.Ltd. Changan UK R&D Centre Limited

In this study,a triple spark ignition scheme was first designed on a three-cylinder 1.5-L dedicated hybrid engine（DHE）.On this basis,the effect of different ignition modes on engine combustion and emission characteristics was studied,especially under high dilution *** results tested at 2000 r/min and 0.8 MPa BMEP（brake mean effective pressure） show that with highly increased in-cylinder flow intensity,using only passive prechamber（PPC） has a lower lean limit than that with single central spark plug（CSP）,thereby leading to slightly higher minimum fuel consumption and nitrogen oxides（NOx） *** side spark plugs（SSP）based on PPC can result in improved capability of lean limit extension and engine performance than ***,the improvement level is lower than that with triple spark plugs（TSP）.As the excess air ratio λincreases,the advantage of PPC and PPC with SSP in improving the combustion phasing compared with CSP gradually ***,the increasing tendency of their ignition delay and combustion duration is more *** added SSP based on PPC can effectively shorten the ignition delay of leaner mixture,but the combustion duration can be only slightly *** a result,under extremely lean condition,the advantage of PPC and PPC with SSP in terms of combustion characteristics over CSP becomes much *** contrast,the TSP ignition can achieve much shorter ignition delay and combustion duration simultaneously under this *** to the highest available dilution level,the TSP ignition achieves the lowest raw ***,it can also reduce the raw carbon monoxide（CO） and hydrocarbons（HC） emissions compared to CSP due to a more thorough combustion of the end gas *** on the excellent performance of TSP,the highest engine brake thermal efficiency（BTE） was further *** results show that with normal RON 92 fuel,the engine finally achieved 43.69% and 45.02% BTE under stochiometric mode with exhaust

关键词： dedicated hybrid engine high dilution condition NOx emissions passive prechamber thermal efficiency triple spark plug scheme

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