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Identification of QTLs associated with cadmium concentration in rice grains

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Journal of Integrative Agriculture 2018年第7期17卷 1563-1573页

作者： HU Da-wei SHENG Zhong-hua LI Qian-long CHEN wei wei xiang-jin XIE Li-hong JIAO Gui-ai SHAO Gao-neng WANG Jian-long TANG Shao-qing HU Pei-song State Key Laboratory of Rice Biology Ministry of Science and Technology/Key Laboratory of Rice Biology and Breeding Ministry of Agriculture/China National Rice Research Institute College of Agronomy Hunan Agricultural University

Cadmium（Cd） contamination in rice has been a hot topic of research because of its potential risk to human health. In this study, a double haploid（DH） population derived from Zhongjiazao 17（YK17）（an early-season indica cultivar）×D50（a tropical japonica cultivar） was used to identify quantitative trait loci（QTLs） associated with Cd concentration in brown rice（CCBR） and Cd concentration in milled rice（CCMR）. Continuous and wide variation for CCBR and CCMR were observed among the DH population. Correlation analysis revealed a positive and highly significant correlation between the two traits. A total of 18 QTLs for CCBR and 14 QTLs for CCMR were identified in five different pot and field trials. Two pairs of QTLs for CCBR（qCCBR2-1 and qCCBR2-2, qCCBR9-1 and qCCBR9-2） and one pair of QTLs for CCMR（qCCMR5-1 and qCCMR5-2） were detected in multiple trials. The alleles increasing CCBR at the qC CBR2-1/qC CBR2-2 and qC CBR9-1/qC CBR9-2 QTLs were contributed by YK17 and D50, respectively, whereas the D50 allele at the qCCMR5-1/qCCMR5-2 QTLs increased CCMR. Eight pairs of QTLs for CCBR and CCMR, qCCBR2-2 and qCCMR2-2, qCCBR3 and qCCMR3, qCCBR4-2 and qCCMR4-1, qCCBR4-3 and qCCMR4-2, qCCBR4-4 and qCCMR4-3, qCCBR5 and qCCMR5-2, qCCBR7 and qCCMR7, and qCCBR11-1 and qCCMR11-2, co-localized on chromosomes 2, 3, 4, 5, 7, and 11, respectively. For all of these QTL pairs, except qCCBR5/qCCMR5-2, the additive effects came from YK17. In addition, four CCMR QTLs showing significant additive×environment interaction and two pairs of CCMR QTLs with bi-allelic epistatic interactions were identified. The results of this study could facilitate marker-assisted selection of breeding rice varieties with low Cd accumulation in grain.

关键词： Cd concentration in grain QTL mapping brown rice milled rice

Phenotype of Rice Floury Endosperm Mutant flo7 and Fine Mapping of Mutated Gene

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Rice science 2015年第4期22卷 162-170页

作者： SHENG Zhong-hua FANG Peng-fei LI San-feng JIAO Gui-ai XIE Li-hong HU Pei-song TANG Shao-qing wei xiang-jin State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Genetic Breeding of Ministry of Agriculture China National Rice Research Institute

The major storage substance in rice endosperm is starch, which accounts for 80% of dry matter weight. In this study, rice mutant flo7, selected from the progeny of Nipponbare＇s tissue culture, displayed floury and opaque endosperm. Compared with its corresponding wild type （WT） Nipponbare, the mutant rio7 produced longer, narrower, thinner and lighter grains. The levels of glucose, fructose and sucrose in the mutant flo7 endosperm were higher than those in the WT endosperm, whereas the protein content was not affected. With respect to both amylose content and gel consistency, the mutant flo7 was lower than WT, but its alkali value was higher. Scanning electron microscopic examinations showed that the endosperm of the mutant flo7 contained irregular, loosely packed and compound starch granules. Genetic analysis indicated that the mutant phenotype was determined by a single recessive nuclear gene The flo7 locus was mapped to a region on the long arm of chromosome 12, within a 95.1 kb interval defined by the markers C2-11 and C5-15. There are 13 open reading frames in the mapping interval. Transcription profiling of the developing grains showed that a number of genes involved in starch synthesis were affected differently in the mutant flo7.

关键词： flo7 floury endosperm starch fine mapping rice mutant

Mapping quantitative trait loci associated with starch paste viscosity attributes by using double haploid populations of rice (Oryza sativa L.)

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Journal of Integrative Agriculture 2020年第7期19卷 1691-1703页

作者： Tahmina SHAR SHENG Zhong-hua Umed ALI Sajid FIAZ wei xiang-jin XIE Li-hong JIAO Gui-ai Fahad ALI SHAO Gao-neng HU Shi-kai HU Pei-song TANG Shao-qing State Key Laboratory of Rice Biology/Key Laboratory of Rice Biology and Breeding Ministry of Agriculture/China National Rice Research InstituteHangzhou 310006P.R.China

The paste viscosity attributes of starch,measured by rapid visco analyzer(RVA),are important factors for the evaluation of the cooking and eating qualities of rice in breeding *** determine the genetic roots of the paste viscosity attributes of rice grains,quantitative trait loci(QTLs)associated with the paste viscosity attributes were mapped,using a double haploid(DH)population derived from Zhongjiazao 17(YK17),a super rice variety,crossed with D50,a tropic japonica ***-four QTLs,for seven parameters of the RVA profiles,were identified in three planting *** 54 QTLs were located on all of the 12 chromosomes,with a single QTL explaining 5.99 to 47.11%of phenotypic *** the QTLs identified,four were repeatedly detected under three environmental conditions and the other four QTLs were repeated under two *** of the QTLs detected for peak viscosity(PKV),trough viscosity(TV),cool paste viscosity(CPV),breakdown viscosity(BDV),setback viscosity(SBV),and peak time(PeT)were located in the interval of RM 6775-RM 3805 under all three environmental conditions,with the exception of pasting temperature(PaT).For digenic interactions,eight QTLs with six traits were identified for additivexenvironment interactions in all three planting *** epistatic interactions were estimated only for PKV,SBV and *** present study will facilitate further understanding of the genetic architecture of eating and cooking quality(ECQ)in the rice quality improvement program.

关键词： RVA profiles eating and cooking quality(ECQ) quantitative trait loci(QTL) DH population rice

Genetic Analysis and Molecular Mapping of Novel White Striped Leaf Mutant Gene in Rice

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Rice science 2013年第5期20卷 336-342页

作者： SHENG Zhong-hua wei xiang-jin SHAO Gao-neng SONG Jian LUO Ju HU Pei-song TANG Shao-qing CHEN Li-yun Rice Research Institute Hunan Agricultural University China National Rice Research Institute

A new white striped leaf mutant wsll was discovered from Nipponbare mutated by ethyl methanesulfonate. The mutant showed white striped leaves at the seedling stage and the leaves gradually turned green after the tillering stage. The chlorophyll content of wsll was significantly lower than that of wild-type during the fourth leaf stage, tillering stage and booting stage. The numbers of chloroplast, grana and grana lamella were reduced and the thylakoids were degenerated in wsll compared with wild type. Genetic analysis showed that the wsll was controlled by a single recessive gene. Molecular mapping of the wsll was performed using an F2 population derived from wsll/Nanjing 11. The wsll was finally mapped on the telomere region of chromosome 9 and positioned between simple sequence repeat markers RM23742 and RM23759 which are separated by approximately 486.5 kb. The results may facilitate map-based cloning of wsll and understanding of the molecular mechanism of the regulation of leaf-color by WSL1 in rice.

关键词： rice white striped leaf mutant genetic analysis gene mapping

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基于知识溢出过程的区域创业体系模型构建

产经评论 2015年第3期6卷 67-80页

作者：陶锋韦锦祥陈秀英暨南大学产业经济研究院

对新经济周期产业升级和经济增长起重要推动力作用的知识型创业,具有创造性破坏的明显特征。由于知识溢出的本地化属性,知识型创业在地区之间存在明显的分异现象。新知识生产速度决定区域内潜在创业机会,而由各种结构性和体制性障碍构成... 详细信息

对新经济周期产业升级和经济增长起重要推动力作用的知识型创业,具有创造性破坏的明显特征。由于知识溢出的本地化属性,知识型创业在地区之间存在明显的分异现象。新知识生产速度决定区域内潜在创业机会,而由各种结构性和体制性障碍构成的"知识过滤器"又限制着创业机会的商业化开发。作为知识型创业一种特别类型的知识溢出创业是新知识从现有组织中溢出并实现商业化的过程,其中关键在于穿透"知识过滤器"。穿透过程依赖于特定空间情境中的知识溢出渠道,更依赖于潜在创业者的吸收能力。基于知识溢出过程构建区域创业体系模型,从理论上解释"知识溢出创业"活动的空间差异。根据隐性知识溢出的空间粘性,着重讨论空间集聚效应、社会网络效应、人力资本流动等三种知识溢出渠道对知识溢出创业过程的影响,同时论证了将扩展后的吸收能力纳入企业家群体构建维度的合理性。在此基础上,识别出区域创业体系的三类核心驱动力,分析了不同情形下区域创业体系的演进动态。对策建议中提出应从全面强调扩大研发投入转向关注疏通知识溢出渠道,增强创业者知识吸引能力。

关键词：区域创业体系知识溢出创业知识过滤器知识溢出渠道创业者吸收能力

Follistatin Inhibited Angiogenin-stimulated rRNA Transcription

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Follistatin Inhibited Angiogenin-stimulated rRNA Transcripti...

Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord

2008年全国生物化学与分子生物学学术大会

作者： xiang-wei Gao jin-feng Su Jia-cheng Tang Zheng-ping Xu (Bioelectromagnetics Laboratory,Zhejiang University School of Medicine,Hangzhou 310058,China)

Angiogenin can translocate into the nucleus in endothelial cells,smooth muscle cells as well as cancer cells and stimulate ribosome RNA (rRNA) transcription,which has been shown to be necessary for the angiogenesis an...

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Neural Regeneration Research 2025年第11期20卷 3302-3316页

作者： Yali Chen Yiyong wei jin Liu Tao Zhu Cheng Zhou Donghang Zhang Department of Anesthesiology West China HospitalSichuan UniversityChengduSichuan ProvinceChina Laboratory of Anesthesia and Critical Care Medicine National-Local Joint Engineering Research Center of Translational Medicine of AnesthesiologyWest China HospitalSichuan UniversityChengduSichuan ProvinceChina Department of Anesthesiology Longgang District Maternity&Child Healthcare Hospital of Shenzhen City(Longgang Maternity and Child Institute of Shantou University Medical College)ShenzhenGuangdong ProvinceChina Department of Anesthesiology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Glial cells play crucial roles in regulating physiological and pathological functions,including sensation,the response to infection and acute injury,and chronic neurodegenerative *** cells include astrocytes,microglia,and oligodendrocytes in the central nervous system,and satellite glial cells and Schwann cells in the peripheral nervous *** the greater understanding of glial cell types and functional heterogeneity achieved through single-cell and single-nucleus RNA sequencing in animal models,few studies have investigated the transcriptomic profiles of glial cells in the human spinal ***,we used high-throughput single-nucleus RNA sequencing and spatial transcriptomics to map the cellular and molecular heterogeneity of astrocytes,microglia,and oligodendrocytes in the human spinal *** explore the conservation and divergence across species,we compared these findings with those from *** the human spinal cord,astrocytes,microglia,and oligodendrocytes were each divided into six distinct transcriptomic *** the mouse spinal cord,astrocytes,microglia,and oligodendrocytes were divided into five,four,and five distinct transcriptomic subclusters,*** comparative results revealed substantial heterogeneity in all glial cell types between humans and ***,we detected sex differences in gene expression in human spinal cord glial ***,in all astrocyte subtypes,the levels of NEAT1 and CHI3L1 were higher in males than in females,whereas the levels of CST3 were lower in males than in *** all microglial subtypes,all differentially expressed genes were located on the sex *** addition to sex-specific gene differences,the levels of MT-ND4,MT2A,MT-ATP6,MT-CO3,MT-ND2,MT-ND3,and MT-CO_(2) in all spinal cord oligodendrocyte subtypes were higher in females than in ***,the present dataset extensively characterizes glial cell heterogeneity and offers a valuable resource for explo

关键词： astrocyte glial cell human microglia oligodendrocyte sex differentiation single-nucleus RNA sequencing spatial transcriptomics species variation spinal cord

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土工格室防护边坡抗冲刷规律试验研究

水土保持通报 2013年第5期33卷 69-72页

作者：王广月孙秀玲魏金祥山东大学土建与水利学院山东济南250061

采用变坡试验水槽对土工格室防护坡面的侵蚀规律进行了试验研究。试验坡度为5°～35°，放水流量1～2m2/h。基于传统的径流量和产沙量测定方法，分析了土工格室边坡坡面的产流产沙演化规律。研究结果表明：土工格室的存在形成一系列沿... 详细信息

采用变坡试验水槽对土工格室防护坡面的侵蚀规律进行了试验研究。试验坡度为5°～35°，放水流量1～2m2/h。基于传统的径流量和产沙量测定方法，分析了土工格室边坡坡面的产流产沙演化规律。研究结果表明：土工格室的存在形成一系列沿格室边缘菱形分布的纹沟，与自然坡面的冲刷形态有本质区别；累计侵蚀量随坡度、流量的增大而增大；土工格室坡面的侵蚀主要发生在前10mn，相同时段内的侵蚀量逐渐减小；同一流量条件下，土工格室坡面冲刷的侵蚀模数与边坡坡度之间呈良好的幂函数正相关关系。

关键词：土工格室坡面累计侵蚀量侵蚀模数

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浅谈岩体分类现状与存在问题

水利科技与经济 2001年第1期7卷 42-43页

作者：金珉善魏向金黑龙江省水利水电勘测设计研究院黑龙江哈尔滨150080

简明扼要地介绍了岩体分类的现状、方法与存在问题。目前世界上有上百种的岩体分类方法 ,无非是各部门按其需要与目的将岩体分类的诸因素重新组合起来制定出该部门的分类方法与基准。

关键词：岩体分类强度渗透物理力学目标专家系统