检索结果-南通市图书馆

Molecular mechanism of a coastal cyanobacterium Synechococcus *** 7002 adapting to changing phosphate concentrations

Marine Life Science & Technology 2024年第3期6卷 562-575页

作者： Qiao-wei Sun Yu Gao Jordan wang Fei-xue Fu Cheng-Wen Yong Shuang-Qing Li Hai-Long Huang wei-Zhong Chen Xin-wei wang Hai-Bo Jiang School of Marine Sciences Ningbo UniversityNingbo 315211China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519080China School of Life Sciences Central China Normal UniversityWuhan 430079China Department of Biological Sciences University of Southern CaliforniaLos AngelesCA 90089USA

Phosphorus concentration on the surface of seawater varies greatly with different environments,especially in *** molecular mechanism by which cyanobacteria adapt to fluctuating phosphorus bioavailability is still *** this study,transcriptomes and gene knockouts were used to investigate the adaptive molecular mechanism of a model coastal cyanobacterium Synechococcus *** 7002 during periods of phosphorus starvation and phosphorus recovery(adding sufficient phosphorus after phosphorus starvation).The findings indicated that phosphorus deficiency affected the photosynthesis,ribosome synthesis,and bacterial motility pathways,which recommenced after phosphorus was *** more,most of the metabolic pathways of cyanobacteria were enhanced after phosphorus recovery compared to the control which was kept in continuous phosphorus replete *** on transcriptome,54 genes potentially related to phosphorusdeficiency adaptation were selected and knocked out individually or in *** was found that five mutants showed weak growth phenotype under phosphorus deficiency,indicating the importance of the genes(A0076,A0549-50,A1094,A1320,A1895)in the adaptation of phosphorus *** mutants were found to grow better than the wild type under phosphorus deficiency,suggesting that the products of these genes(A0079,A0340,A2284–86)might influence the adaptation to phosphorus *** analysis revealed that cyanobacteria exposed to highly fluctuating phosphorus concentrations have more sophisticated phosphorus acquisition *** results elucidated that Synechococcus *** 7002 have variable phosphorus response mechanisms to adapt to fluctuating phosphorus concentration,providing a novel perspective of how cyanobacteria may respond to the complex and dynamic environments.

关键词： Synechococcus sp PCC 7002 Phosphorus fluctuation Cyanobacteria ・Gene knockout Molecular mechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

New Strategy for Boosting Cathodic Performance of Protonic Ceramic Fuel Cells Through Incorporating a Superior Hydronation Second Phase

引用

Energy & Environmental Materials 2024年第4期7卷 83-92页

作者： Chuan Zhou Xixi wang Dongliang Liu Meijuan Fei Jie Dai Daqin Guan Zhiwei Hu Linjuan Zhang Yu wang wei wang Ryan O'Hayre San Ping Jiang wei Zhou Meilin Liu Zongping Shao State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical Engineering Nanjing Tech University Max Planck Institute for Chemical Physics of Solids Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences Department of Metallurgical and Materials Engineering Colorado School of Mines WA School of Mines: Minerals Energy and Chemical Engineering (WASM-MECE) Curtin University Suzhou Laboratory School of Materials Science and Engineering Georgia Institute of Technology

For protonic ceramic fuel cells,it is key to develop material with high intrinsic activity for oxygen activation and bulk proton conductivity enabling water formation at entire electrode ***,a higher water content which benefitting for the increasing proton conductivity will not only dilute the oxygen in the gas,but also suppress the O2adsorption on the electrode surface.

关键词： cathode high-hydrating capability proton conductivity protonic ceramic fuel cells

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

YOLO-DD:Improved YOLOv5 for Defect Detection

引用

Computers, Materials & Continua 2024年第1期78卷 759-780页

作者： Jinhai wang wei wang Zongyin Zhang Xuemin Lin Jingxian Zhao Mingyou Chen Lufeng Luo School of Electronic and Information Engineering Foshan UniversityFoshan528000China School of Mechatronic Engineering and Automation Foshan UniversityFoshan528000China

As computer technology continues to advance,factories have increasingly higher demands for detecting ***,detecting defects in a plant environment remains a challenging task due to the presence of complex backgrounds and defects of varying shapes and *** address this issue,this paper proposes YOLO-DD,a defect detectionmodel based on YOLOv5 that is effective and *** improve the feature extraction process and better capture global information,the vanilla YOLOv5 is augmented with a new module called Relative-Distance-Aware Transformer(RDAT).Additionally,an Information Gap Filling Strategy(IGFS)is proposed to improve the fusion of features at different *** classic lightweight attention mechanism Squeeze-and-Excitation(SE)module is also incorporated into the neck section to enhance feature expression and improve the model’s *** results on the NEU-DET dataset demonstrate that YOLO-DDachieves competitive results compared to state-of-the-art methods,with a 2.0% increase in accuracy compared to the original YOLOv5,achieving 82.41% accuracy and38.25FPS(framesper second).Themodel is also testedon a self-constructed fabric defect dataset,and the results show that YOLO-DD is more stable and has higher accuracy than the original YOLOv5,demonstrating its stability and generalization *** high efficiency of YOLO-DD enables it to meet the requirements of industrial high accuracy and real-time detection.

关键词： YOLO-DD defect detection feature fusion attention mechanism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diagnostic Value of GDF10 for the Tumorigenesis and Immune Infiltration in Lung Squamous Cell Carcinoma

引用

Current Medical Science 2024年第2期44卷 309-327页

作者： Xiao-jun wang Jia-ping CHEN Xin-wei QIAO wang-yang MENG Yang-wei wang Yun-chong MENG Rong ZHAO wei LIN Yong-de LIAO Han XIAO Pei-yuan MEI Department of Thoracic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Thoracic Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021China

Objective:Lung squamous cell carcinoma(LUSC)is associated with a low survival *** suggests that bone morphogenetic proteins(BMPs)and their receptors(BMPRs)play crucial roles in tumorigenesis and ***,a comprehensive analysis of their role in LUSC is *** study aimed to explore the relationship between BMPs/BMPRs expression levels and the tumorigenesis and prognosis of ***:The“R/Limma”package was utilized to analyze the differential expression characteristics of BMPs/BMPRs in LUSC,using data from TCGA,GTEx,and GEO ***,the“survminer”packages were employed to investigate their prognostic value and correlation with clinical features in *** core gene associated with LUSC progression was further explored through weighted gene correlation network analysis(WGCNA).LASSO analysis was conducted to construct a prognostic risk model for *** specimens were examined by immunohistochemical analysis to confirm the diagnostic value in ***,based on the tumor immune estimation resource database and tumor-immune system interaction database,the role of the core gene in the tumor microenvironment of LUSC was ***:GDF10 had a significant correlation only with the pathological T stage of LUSC,and the protein expression level of GDF10 decreased with the tumorigenesis of LUSC.A prognostic risk model was constructed with GDF10 as the core gene and 5 hub genes(HRASLS,HIST1H2BH,FLRT3,CHEK2,and ALPL)for ***10 showed a significant positive correlation with immune cell infiltration and immune checkpoint ***:GDF10 might serve as a diagnostic biomarker reflecting the tumorigenesis of LUSC and regulating the tumor immune microenvironment to guide more effective treatment for LUSC.

关键词： lung squamous cell carcinoma tumorigenesis bone morphogenetic protein GDF10 tumor immune microenvironment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Jetting of a near-wall cavitation bubble induced by another tandem bubble

引用

Journal of Hydrodynamics 2024年第3期36卷 444-456页

作者： Hui Han Jing-zhu wang Jian-lin Huang Peng-bo Bai Yong-gang Chen Yi-wei wang Key Laboratory for Mechanics in Fluid Solid Coupling Systems Institute of MechanicsChinese Academy of SciencesBeijing100190China School of Engineering Science University of Chinese Academy of SciencesBeijing100049China Guangdong Aerospace Research Academy Guangzhou511458China School of Future Technology University of Chinese Academy of SciencesBeijing100049China Jianghuai Advance Technology Center Hefei230088China

Double bubbles near a rigid wall surface collapse to produce a significant jet impact,with potential applications in surface cleaning and ultrasonic ***,the dynamic behaviors of near-wall bubbles remain *** this study,we investigate the jetting of a near-wall bubble induced by another tandem *** define two dimensionless standoff distances,γ_(1),γ_(2),to represent the distances from the center of the near-wall bubble to the rigid wall and the center of controlling bubble to the center of the near-wall bubble,*** observations reveal three distinct jetting regimes for the near-wall bubble:transferred jetting,double jetting,and directed *** further investigate the jetting mechanism,numerical simulations are conducted using the compressibleInterFoam solver in the open-source framework of OpenFOAM.A detailed analysis shows that the transferred jet flow is caused by the pinch-off resulting from the axial contraction velocity at the lower end of the near-wall bubble being greater than the vertical contraction velocity,leading to a maximum jet velocity of 682.58 m/*** the case of double jetting,intense stretching between the controlling bubble and the wall leads to a pinch-off and a double jetting with a maximum velocity of 1096.29 m/*** directed jet flow is caused by the downward movement of the high-pressure region generated by the premature collapse of the controlling bubble,with the maximum jet velocity reaching 444.62 m/s.

关键词： Jetting of near-wall bubble controlling bubble impact velocity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

引用

World Journal of Stem Cells 2024年第2期16卷 163-175页

作者： Yan Ren Yan-Ni Cui Hong-wei wang The Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi ProvinceTaiyuan 030001Shanxi ProvinceChina

BACKGROUND In vitro expansion to increase numbers of hematopoietic stem cells(HSCs)in cord blood could improve clinical efficacy of this vital ***(NAM)can promote HSC expansion ex vivo,but its effect on hematopoietic stem and progenitor cells(HSPCs,CD34^(+)CD38)and functional subtypes of HSCs-shortterm repopulating HSCs(ST-HSCs,CD34^(+)CD38CD45RACD49f^(+))and long-term repopulating HSCs(LT-HSCs,CD34^(+)CD38CD45RACD49f^(+)CD90^(+))is not yet *** a sirtuin 1(SIRT1)inhibitor,NAM participates in regulating cell adhesion,polarity,migration,proliferation,and ***,SIRT1 exhibits dual effects by promoting or inhibiting differentiation in different tissues or *** propose that the concentration of NAM may influence proliferation,differentiation,and SIRT1 signaling of *** To evaluate the effects and underlying mechanisms of action of different concentrations of NAM on HSC proliferation and *** CD34^(+)cells were purified from umbilical cord blood using MacsCD34 beads,and cultured for 10-12 d in a serum-free medium supplemented with cytokines,with different concentrations of NAM added according to experimental *** cytometry was used to detect phenotype,cell cycle distribution,and apoptosis of the cultured ***-time polymerase chain reaction was used to detect the transcription levels of target genes encoding stemness-related factors,che mokines,components of hypoxia pathways,and antioxidant ***-dihydro-fluorescein diacetate probes were used to evaluate intracellular production of reactive oxygen species(ROS).Determination of the effect of different culture conditions on the balance of cytokine by cytometric bead *** Compared with the control group,the proportion and expansion folds of HSPCs(CD34^(+)CD38)incubated with 5 mmol/L or 10 mmol/L NAM were significantly increased(all P<0.05).The ST-HSCs ratio and fold expansion of the 5 mmol/L NAM group were significantly

关键词： Hematopoietic stem cells Nicotinamide Concentration Proliferation Differentiation Sirtuin 1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Orthogonal waveform design with fractional programming on the ambiguity suppression of SAR systems

引用

Science China(Information Sciences) 2024年第9期67卷 263-279页

作者： Yunkai DENG Yongwei ZHANG Zhimin ZHANG wei wang Heng ZHANG Department of Space Microwave Remote Sensing System Aerospace Information Research InstituteChinese Academy of Sciences

Waveform diversity(WD) represents a dynamic and transformative technology widely used in radar systems to enhance sensitivity and discrimination capabilities. Recently, WD techniques have been extensively explored for their potential ambiguity suppression within synthetic aperture radar(SAR) *** these, the alternate transmitting mode combined with orthogonal waveforms emerges as a particularly promising solution. This study focuses on optimizing the power spectrum density(PSD) of signals to design and generate an orthogonal waveform pair that achieves both a low cross-correlation-to-autocorrelation ratio(CAR) and satisfactory imaging performance. Initially, we construct a fractional programming model with convex constraints to minimize the CAR. To address this challenge, we introduce an iterative optimization procedure for the PSD variable, which sequentially reduces the CAR. Each optimization step can be efficiently solved using a quadratically constrained quadratic program, ensuring that the resulting computational complexity remains low. Building on the optimized PSD, we established a parametric piecewise linear model to generate an orthogonal waveform pair. This model not only maintains a low CAR but achieves satisfactory imaging performance in real-time applications. Consequently, this orthogonal waveform pair effectively suppresses range ambiguity in SAR systems. Finally, we demonstrated the practicability and effectiveness of the proposed orthogonal waveforms through detailed simulation experiments, specifically targeting ambiguity suppression in conventional quad-polarization SAR systems.

关键词： synthetic aperture radar (SAR) waveform diversity (WD) ambiguity suppression orthogonal waveform fractional programming power spectrum density (PSD)

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

QoS Routing Optimization Based on Deep Reinforcement Learning in SDN

引用

Computers, Materials & Continua 2024年第5期79卷 3007-3021页

作者： Yu Song Xusheng Qian Nan Zhang wei wang Ao Xiong State Key Laboratory of Networking and Switching Technology Beijing University of Posts and TelecommunicationsBeijing100876China Marketing Service Center State Grid Jiangsu Electric Power Co.Ltd.Nanjing220000China Customer Service Center State Grid Co.Ltd.Nanjing211161China

To enhance the efficiency and expediency of issuing e-licenses within the power sector, we must confront thechallenge of managing the surging demand for data traffic. Within this realm, the network imposes stringentQuality of Service (QoS) requirements, revealing the inadequacies of traditional routing allocation mechanismsin accommodating such extensive data flows. In response to the imperative of handling a substantial influx of datarequests promptly and alleviating the constraints of existing technologies and network congestion, we present anarchitecture forQoS routing optimizationwith in SoftwareDefinedNetwork (SDN), leveraging deep reinforcementlearning. This innovative approach entails the separation of SDN control and transmission functionalities, centralizingcontrol over data forwardingwhile integrating deep reinforcement learning for informed routing decisions. Byfactoring in considerations such as delay, bandwidth, jitter rate, and packet loss rate, we design a reward function toguide theDeepDeterministic PolicyGradient (DDPG) algorithmin learning the optimal routing strategy to furnishsuperior QoS provision. In our empirical investigations, we juxtapose the performance of Deep ReinforcementLearning (DRL) against that of Shortest Path (SP) algorithms in terms of data packet transmission delay. Theexperimental simulation results show that our proposed algorithm has significant efficacy in reducing networkdelay and improving the overall transmission efficiency, which is superior to the traditional methods.

关键词： Deep reinforcement learning SDN route optimization QoS

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Design and Performance Analysis of HMDV Dynamic Inertial Suspension Based on Active Disturbance Rejection Control

引用

Computer Modeling in Engineering & Sciences 2024年第8期140卷 1485-1506页

作者： Xiaofeng Yang wei wang Yujie Shen Changning Liu Tianyi Zhang School of Automotive and Traffic Engineering Jiangsu UniversityZhenjiang212013China Automotive Engineering Research Institute Jiangsu UniversityZhenjiang212013China Chongqing Key Laboratory of Urban Rail Transit System Integration and Control Chongqing400074China State Key Laboratory of Advanced Design and Manufacturing Technology for Vehicle Hunan universityChangsha410082China

This paper addresses the impact of vertical vibration negative effects,unbalanced radial forces generated by the static eccentricity of the hub motor,and road excitation on the suspension performance of Hub Motor Driven Vehicle(HMDV).A dynamic inertial suspension based on Active Disturbance Rejection Control(ADRC)is proposed,combining the vertical dynamic characteristics of dynamic inertial suspension with the features of ADRC,which distinguishes between internal and external disturbances and arranges the transition ***,a simulation model of the static eccentricity of the hub motor is established to simulate the unbalanced radial electromagnetic force generated under static eccentricity.A quarter-vehicle model of an HMDV with a controllable dynamic inertial suspension is then ***,the passive suspension model is studied under different grades of road excitation,and the impact mechanism of suspension performance at speeds of 0–20 m/s is ***,the three main components within the ADRC controller are designed for the second-order controlled system,and optimization algorithms are used to optimize its internal ***,the performance of the traditional passive suspension,the PID-based controllable dynamic inertial suspension,and the ADRC-based controllable dynamic inertial suspension are analyzed under different road *** results show that,under sinusoidal road input,the ADRC-based controllable dynamic inertial suspension exhibits a 52.3%reduction in the low-frequency resonance peak in the vehicle body acceleration gain diagram compared to the traditional passive suspension,with significant performance optimization in the high-frequency *** random road input,the ADRC-based controllable dynamic inertial suspension achieves a 29.53%reduction in the root mean square value of vehicle body acceleration and a 14.87%reduction in dynamic tire *** indicates that the designed controllable dynamic inertia

关键词： HMDV static eccentricity ADRC dynamic inertial suspension road excitation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MicroRNA let-7 targets BmCDK1 to regulate cell proliferation and endomitosis of silk gland in the silkworm,Bombyx mori

引用

Insect Science 2024年第4期31卷 1026-1040页

作者： wei wang Linshengzhe Ji Xinyuan Jing Ping Zhao Qingyou Xia Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City Biological Science Research CenterSouthwest UniversityChina Key Laboratory for Germplasm Creation in Upper Reaches of the Yangtze River Ministry of Agriculture and Rural AffairsChongqingChina Engineering Laboratory of Sericultural and Functional Genome and Biotechnology Development and Reform CommissionChongqingChina

MicroRNAs play critical roles in multiple developmental processes in *** previous study showed that CRISPR/Cas9-mediated knock down of the microRNA let-7 in silkworms increased the size of larvae and silk glands,thereby improving the silk production *** this study,we elucidate the molecular mechanism underlying of let-7 regulates *** of differentially expressed genes in response to let-7 knock down revealed enrichment of pathways associated with cell proliferation and DNA ***-7 dysregulation affected the cell cycle and proliferation of the Bombyx mori cell line ***-luciferase and target site mutation assays showed that BmCDK1 is a direct target gene of let-7,with only 1 binding site on its 3′-untranslated *** interference of BmCDK1 inhibited cell proliferation,but this effect was counteracted by co-transfection with let-7 ***,let-7 knock down induced BmCDK1 expression and promoted cell proliferation in multiple tissues,and further induced endomitosis in the silk gland in *** down of BmCDK1 resulted in abnormal formation of a new epidermis,and larval development was arrested at the 2nd or 3rd molt *** together,our results demonstrated that BmCDK1 is a novel target of let-7 in cell fate determination,possessing potential for improving silk yield in silkworm.

关键词： BmCDKI CRISPR/Cas9 cell proliferation let-7 silkworm

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：