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Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH

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Journal of Integrative Plant Biology 2013年第7期55卷 654-662页

作者： Qiong Wu Fang Liu Shaohui Li Guoli Song Chunying wang Xiangdi Zhang Yuhong wang David Stelly kunbo wang Cotton Research Institute the Chinese Academy of Agricultural Sciences/National Key Laboratory of Cotton Biology Haikou Experimental Station Chinese Academy of Tropical Agricultural Sciences Department of Soil and Crop Sciences Texas A&M UniversityCollege Station

Gossypium mustelinum （（AD）4） is one of five disomic species in Gossypium. Three 45S ribosomal DNA （rDNA） loci were detected in （AD）4 with 45S rDNA as probe, and three pairs of brighter signals were detected with genomic DNA （gDNA） of Gossypium D genome species as probes. The size and the location of these brighter signals were the same as those detected with 45S rDNA as probe, and were named GISH-NOR. One of them was super-major, which accounted for the fact that about one-half of its chromosome at metaphase was located at chromosome 3, and other two were minor and located at chromosomes 5 and 9, respectively. All GISH-NORs were located in A sub-genome chromosomes, separate from the other four allopolyploid cotton species. GISH-NOR were detected with D genome species as probe, but not A. The greatly abnormal sizes and sites of （AD）4 NORs or GISH-NORs indicate a possible mechanism for 45S rDNA diversification following （AD）4 speciation. Comparisons of GISH intensities and GISH-NOR production with gDNA probes between A and D genomes show that the better relationship of （AD）4 is with A genome. The shortest two chromosomes of A sub-genome of G. mustelinum were shorter than the longest chromosome of D sub-genome chromosomes. Therefore, the longest 13 chromosomes of tetraploid cotton being classified as A sub-genome, while the shorter 13 chromosomes being classified as D sub-genome in traditional cytogenetic and karyotype analyses may not be entirely correct.

关键词： Chromosomes cotton Gossypium mustelinum in situ hybridization nucleolar organizer region genome.

Primary analysis of QTG contribution to heterosis in upland cotton

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Chinese Science Bulletin 2010年第26期55卷 2956-2965页

作者： ZHANG XianLiang LIU Fang wang Wei LI ShaoHui wang ChunYing ZHANG XiangDi wang YuHong wang kunbo Cotton Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture of People's Republic of China Anyang 455000 China Kaifeng Research Academy of Agriculture and Forestry Key Laboratory of Agriculture Biological Breeding Kaifeng 475141 China Yanhai District Agricultural Research Institute of Jiangsu Province Yancheng 224002 China

In this paper, we analyzed the contribution of pure DNA factors to heterosis using quantitative trait genes (QTG) in two randomly selected strains from a recombinant inbred line of Gossypium hirsutum. According to a set of QTL mapping results, combined with analysis of DNA recombinant fragment sources in the two strains and QTL association analysis with their field traits, we hypothesize a view of "dominance + overdominance + epistasis". That is, additive and additive epistasis may be the genetic basis of heterosis, and dominance, overdominance and epistasis may be the modes of heterosis action. Based on the heterosis results of this study, we also suggest a molecular mechanism for heterosis, and explain, in detail, with two randomly selected strains as examples. The male and female parent-derived additive epistatic QTLs of upper half mean length in LG01 and LG03 produced a trait variance of 2.99-3.52 compared with the female parent-derived loci. The trait of bolls per plant was controlled by two pairs of additive epistatic QTLs in LG02 and LG07, which were derived from both female and male parents. The QTLs were reciprocally interacted and produced a trait variance of 0.86. An initial concept of "super-hybrid cotton" was raised according to the nature of additive effect, that is genetic stability.

关键词：杂种优势利用陆地棉 QTL定位数量性状基因重组自交系显性上位遗传基础随机选择

Designations for individual genomes and chromosomes in Gossypium

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Journal of Cotton Research 2018年第1期1卷 35-39页

作者： wang kunbo WENDEL Jonathan F. HUA Jinping State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Sciences Department of Ecology Evolution and Organismal Biology Iowa State University College of Agronomy and Biotechnology China Agricultural University

Gossypium, as the one of the biggest genera, the most diversity, and the highest economic value in field crops, is assuming an increasingly important role in studies on plant taxonomy, polyploidization, phylogeny, cytogenetics, and genomics. Here we update and provide a brief summary of the emerging picture of species relationships and diversification, and a set of the designations for individual genomes and chromosomes in Gossypium. This cytogenetic and genomic nomenclature will facilitate comparative studies worldwide, which range from basic taxonomic exploration to breeding and germplasm introgression.

关键词： Nomenclature Individual genome Individual chromosome Gossypium

Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum

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Chinese Science Bulletin 2013年第26期58卷 3199-3201页

作者： GAO HaiYan wang XingFen LIU Fang PENG RenHai ZHANG Yan CHENG Hua MA ZhiYing wang kunbo State Key Laboratory of Cotton Biology/Cotton Research Institute Chinese Academy of Agricultural Sciences North China Key Laboratory for Crop Germplasm Resources of Education Ministry Key Laboratory for Crop Germplasm Resources of Hebei Province Agricultural University of Hebei Anyang Institute of Technology

Gossypium herbaceum var. africanum is the only wild cotton species within the cultivated diploid G. herbaceum. As the A sub-genome donor of tetraploid cotton, it is characterized by its resistance to insects, diseases, and other adversities. We have constructed the first bacterial artificial chromosome library (BAC) for G. herbaceum var. africanum. With high quality and broad coverage, this library includes 75000 clones, with an average insert size of about 115 kb and fewer than 4% empty clones. Our library is approximately five-fold the size of the A-genome (1667 Mb) and it provides 99.3% probability for isolating genes of interest or their sequences. Using nine SSR markers that are located on five different chromosomes and linked with resistance to Verticillium wilt, seven of nine could amplify the 40 superpools and got 1-14 hits. Because of its moderate wide coverage and relative large insert size, this library will be an important genomic resource for classifying and analyzing the evolution of cotton species, as well as for isolating disease-resistance genes and control elements.

关键词：细菌人工染色体文库草棉变种非洲 A基因组棉花品种 SSR标记基因序列

Polysaccharides of Aspergillus cristatus attenuate obesity by regulating gut microbiota and gut microbiota-related metabolites

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Food Science and Human Wellness 2024年第3期13卷 1513-1530页

作者： Mingzhi Zhu Bohao Shang Fang Zhou Yong Yuan Feiyan Yin Jin Cao Jianan Huang kunbo wang Xin Zeng Maiquan Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China College of Life Sciences Huaibei Normal UniversityHuaibei 235000China Hunan Tea Group Co. Ltd.Changsha 410128China School of Food Science and Technology Hunan Agricultural UniversityChangsha 410128China

Golden-flower fungus,the only dominant microorganism determining the Fu-brick tea quality through fermentation and the important microbe in Liupao tea,is considered a potential probiotic fungus based on its anti-obesity ***,the classification of golden-flower fungi is still controversial;the anti-obesity effect of golden-flower fungus polysaccharides remains *** this study,we identify a golden-flower strain as Aspergillus cristatus based on morphological characteristics and multigene phylogeny analysis,which resolves the controversy of ***,we find *** polysaccharides(ACPS)attenuate obesity in *** modulate gut bacterial *** which Akkermansia,Akkermansia muciniphila,Bacteroides,Romboutsia,Blautia,and Desulfovibrio are considered the core microbes regulated by *** increase fecal total short-chain fatty acid content and serum,hepatic,and fecal total bile acid ***,ACPS-induced gut microbiota alteration plays a causal role in the protection from obesity,according to a fecal transplantation ***,ACPS ameliorate obesity by regulating gut microbiota and gut microbiota-related metabolites.

关键词： Golden-flower fungus Aspergillus cristatus Polysaccharides Obesity Gut microbiota

Genome-wide identification of OSCA gene family and their potential function in the regulation of dehydration and salt stress in Gossypium hirsutum

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Journal of Cotton Research 2019年第2期2卷 87-104页

作者： YANG Xiu XU Yanchao YANG Fangfang MAGwangA Richard Odongo CAI Xiaoyan wang Xingxing wang Yuhong HOU Yuqing wang kunbo LIU Fang ZHOU Zhongli Institute of Cotton Research Chinese Academy of Agricultural Science (ICRCAAS)/Research Base in Anyang Institute of TechnologyState Key Laboratory of Cotton Biology

Background:Cotton(Gossypium hirsutum) provides the largest natural fiber for the textile manufacturing industries,but its production is on the decline due to the effects of *** salt-alkalization leads to damage in cotton growth and a decrease in ***-gated calcium-permeable channels(OSCA) have been found to be involved in the detection of extracellular changes which trigger an increase in cytosolic free calcium ***-induced calcium ion increases have been widely speculated to be playing a role in osmosensing in ***,the molecular nature of the corresponding calcium ion channels remains *** this research work,we describe the OSCA genes and their putative function in osmosensing in plants by carrying out genomewide identification,characterization and functional analysis of the significantly up-regulated OSCA gene,GhOSCA1.1 through reverse ***:A total of 35,21 and 22 OSCA genes were identified in ***,***,and *** genomes,respectively,and were classified into four different clades according to their gene structure and phylogenetic *** and protein structure analysis indicated that 35 GhOSCA genes contained a conserved RSN17 TM(PF02714) ***,the cis-regulatory element analysis indicated that the OSCA genes were involved in response to abiotic ***,the knockdown of one of the highly up-regulated genes,GhOSCA1.1 showed that the virus-induced gene silenced(VIGS) plants were highly sensitive to dehydration and salinity stresses compared with the none VIGS plants as evident with higher concentration levels of oxidant enzymes compared with the antioxidant enzymes on the leaves of the stressed ***:This study provides the first systematic analysis of the OSCA gene family and will be important for understanding the putative functions of the proteins encoded by the OSCA genes in *** results provide a new insight of de

关键词： OSCA gene family Gossypium hirsurum VIGS Salt and dehydration stress

The morphological diversity of pollen in the genus Gossypium

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Journal of Cotton Research 2023年第1期6卷 48-59页

作者： CAI Xiaoyan HOU Yuqing MUHAMMAD Umer Jawad wang Heng XU Yanchao ZHENG Jie wang Yuhong LIU Fang ZHOU Zhongli HUA Jinping wang kunbo State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000HenanChina National Nanfan Research Institute(Sanya) Chinese Academy of Agriculture SciencesSanya 572025HainanChina College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Background Plant pollen has diverse morphological characteristics that can be consistently passed down from generation to *** on pollen morphology is thus immensely important for plant classification and *** the genus Gossypium,however,in-depth research on pollen morphology is lacking,with only few reports on limited cotton *** evaluate the diversity of pollen in Gossypium,we therefore conducted a comprehensive analysis of the pollen morphology of 33 cotton species and varieties using scanning electron *** The 33 analyzed cotton samples exhibited common pollen morphological features,including spherical shapes,radial symmetry,echination,panporation,and operculation,while the pollen size,spine shape,spine density and length showed distinctive *** size varied significantly among species,with diameters ranging from62.43 μm in *** to 103.41 μm in *** exine had an echinate sculptural texture,and spines were mostly conical or sharply conical but occasionally *** density varied from 173 in *** to 54 in ***,while spine length ranged from 3.53 μm in *** to 9.47 μm in *** addition,the 33cotton species and varieties were grouped at a genetic distance of 3.83 into three *** Ⅰ comprised five allotetraploid AD-genome cotton species,four D-genome species,and one K-genome *** Ⅱ included 13diploid species from A,B,D,E,and G genomes,whereas Cluster Ⅲ only consisted one E-genome species *** Although pollen characteristics alone are not enough to resolve taxonomic and systematic relationships within the genus Gossypium,our results add to knowledge on palynomorphology and contribute to phenological information on these *** findings should aid future systematic and phylogenetic studies of the Gossypium genus.

关键词： Cotton Scanning electron microscopy Pollen morphology Diversity

Identification of QTLs and candidate genes for physiological traits associated with drought tolerance in cotton

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Journal of Cotton Research 2020年第1期3卷 13-45页

作者： MAGwangA Richard Odongo LU Pu KIRUNGU Joy Nyangasi CAI Xiaoyan ZHOU Zhongli AGONG Stephen Gaya wang kunbo LIU Fang State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Jaramogi Oginga Odinga University of Science and Technology P.O Box 210-40601BondoKenya

Background:Cotton is mainly grown for its natural fiber and edible *** fiber obtained from cotton is the indispensable raw material for the textile *** ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its *** of drought stress are estimated to affect more than 50%of the cotton growing *** elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from ***,a drought tolerant donor parent and *** which is highly susceptible to drought ***:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 *** map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,*** stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At ***-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll *** genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined *** genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in *** RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in *** cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton *** detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,***,showed the significant genes harbored by the wild progenitors which

关键词： Cotton spp. Quantitative trait loci Genetic map Drought tolerance mi RNAs Gene ontology

Design of Quasi-planar Ridge SOI Waveguide Resonator for Integrated Optical Gyro

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Design of Quasi-planar Ridge SOI Waveguide Resonator for Int...