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鲟鱼育种及新品种选育研究进展

中山大学学报（自然科学版）（中英文） 2025年第1期64卷 93-105页

作者：王巍宋海亮朱华董颖东天胡红霞北京市农林科学院水产科学研究所暨北京市渔业生物技术重点实验室北京100068 农业农村部“鲟鱼遗传育种”重点实验室浙江杭州311799 国家数字种业创新中心北京100097

鲟鱼是鲟形目鱼类的统称。它们不仅具有重要的进化地位,而且具有重要的经济价值。鲟鱼新品种选育研究在近几十年取得了显著进展。本文综述了鲟鱼新品种选育的主要方法,包括杂交育种、雌核发育、性逆转诱导、基因编辑、家系选育、基因组... 详细信息

鲟鱼是鲟形目鱼类的统称。它们不仅具有重要的进化地位,而且具有重要的经济价值。鲟鱼新品种选育研究在近几十年取得了显著进展。本文综述了鲟鱼新品种选育的主要方法,包括杂交育种、雌核发育、性逆转诱导、基因编辑、家系选育、基因组选择和细胞水平育种,讨论了各技术的应用现状,在育种上相关研究和取得的重要突破,并结合我们育种实践积累的经验,展望鲟鱼育种和种业的未来发展,为未来的鲟鱼育种研究提供理论基础和实践指导。

关键词：鲟鱼育种新品种

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Research on Two-stage Voltage and Reactive Power Optimization Coordinated Control Method Considering Flexible On-load Tap Changer

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Dianwang Jishu/Power System Technology 2025年第1期49卷 272-283页

作者： Li, Jiaming Huo, Qunhai Yin, Jingyuan Lu, Hao Zhou, Jing Zhu, Meisheng He, Wei dong, Hongwei Institute of Electrical Engineering Chinese Academy of Sciences Haidian District Beijing 100192 China School of Electronic Electrical and Communication Engineering University of Chinese Academy of Sciences Shijingshan District Beijing 100049 China Shanghai Jiguang Polytechnic College Baoshan District Shanghai 201901 China Three Gorges Electric Power Co. Ltd. Hubei Province Wuhan 430000 China

With the large-scale integration of distributed photovoltaic (DPV) in the distribution network, voltage exceeding limits and fluctuations are becoming increasingly severe. The voltage and reactive power optimization and coordination control method considering new power quality management devices needs to be further improved to adapt to the new changes in the power grid. This article considers the power quality regulation effect of the new flexible on-load tap changer (OLTC) and proposes a two-stage voltage and reactive power optimization coordinated control method. The first stage is the day before hour level scheduling stage. Based on distributed photovoltaic and load prediction data, the active output results of DPV and the switching results of flexible OLTC taps and capacitor banks are obtained through power flow calculation and iterative optimization. The second stage is the minute-level reactive power optimization stage. Based on the first stage, considering the reactive power output characteristics of flexible OLTC and DPV, adjusting the reactive power output of the equipment while correcting the capacitor bank switching combination in the first stage, further reducing the maximum voltage deviation of each node and making the distribution network voltage distribution more reasonable. An IEEE33 node distribution system simulation model is built in the paper. The proposed two-stage voltage and reactive power optimization coordination control method considering flexible OLTC can improve the DPV absorption level by 9.29% based on the conventional economic optimal goal of 88.07%, while meeting the requirement that the voltage deviation of the entire node is less than 0.1pu During the entire period, the overall economic efficiency improved by 7.8%. The results demonstrate the rationality and effectiveness of the proposed method. © 2025 Power System Technology Press. All rights reserved.

关键词： Quality management

Development of Auto Sheets in Baosteel

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工程科学（英文版） 2005年第2期3卷 30-34页

作者： LuJiangxin wangli BaosteelResearchandDevelopmentCenter Shanghai201900

The development trends of auto sheets in recent years are summarized with regard to the environmental regulations, market demands and strategy of the steel industry. The growth of domestic auto industry and demands for auto sheets are also briefed. The current status of development of Bake hardenable steel, Isotropic steel for outer panels and Transformation induced plasticity steel, dual phase steel for structural parts in Baosteel is introduced and the future of auto sheets in Baosteel is forecasted.

关键词：自动板塑性钢双相钢炼钢技术

生物可降解聚乳酸共衣康酸丁二醇酯/纳米二氧化硅包装材料对发酵酸奶贮藏品质及活菌数的影响

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食品与发酵工业 2025年第1期51卷 108-115页

作者：蒋伟陈钟生张元董同力嘎内蒙古农业大学食品科学与工程学院内蒙古呼和浩特010018

探讨不同阻隔性的聚乳酸共衣康酸丁二醇酯/纳米二氧化硅共聚物薄膜[poly(L-lactic-co-butanediol itaconate)nano-silica copolymer films,PLBI-SiO_(2)]包装对发酵酸奶贮藏效果,并对贮藏期间酸奶的理化指标和活菌数进行测定。结果表明:... 详细信息

探讨不同阻隔性的聚乳酸共衣康酸丁二醇酯/纳米二氧化硅共聚物薄膜[poly(L-lactic-co-butanediol itaconate)nano-silica copolymer films,PLBI-SiO_(2)]包装对发酵酸奶贮藏效果,并对贮藏期间酸奶的理化指标和活菌数进行测定。结果表明:在4℃贮藏0~21 d期间,所有包装组的pH都呈现下降趋势,PLBI-SiO_(2)0.5%包装组与对照(市售)组在贮藏后期pH下降得到减缓;在贮藏期间,PLBI-SiO_(2)0.5%包装组与对照(市售)组能有效维持酸奶的质构特性与持水能力,同时也保持良好的感官评价与表观黏度;所有包装组的活菌数在整个贮藏期间都呈现逐渐降低的趋势,但随包装阻隔性的提高,下降幅度得到减缓,PLBI-SiO_(2)0.5%包装组与对照组在贮藏21 d时,保加利亚乳杆菌与嗜热链球菌都保持在10~7 CFU/mL以上。综合各项指标,PLBI-SiO_(2)0.5%包装组在贮藏21 d可以维持酸奶贮藏品质并几近于市售包装组,这为酸奶及其他发酵乳替代包装材料提供了一定的理论基础。

关键词：酸奶贮藏纳米二氧化硅聚乳酸共衣康酸丁二醇酯纳米二氧化硅共聚物阻隔性

Radiolabeling of N-Succinimidyl-3-[(125)~I]iodobenzoate intermediate

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Nuclear Science and Techniques 2004年第1期15卷 43-45页

作者： wangli-Hua WANGYong-Xian YINDuan-Zhi LIJun-Ling ShanghaiInstituteofAppliedPhysics theChineseAcademyofSciencesShanghai201800

Organostannanes were important precursors which was easy to radioiodinate. N-Succinimidyl-3- (tri-n-butylstannyl) benzoate (STB) was radiolabeled using Iodogen to get radioactive N-Succinimidyl-3-iodobenzo- ate (S125IB) with 96% of high radiochemical yield. The optimization of labeling condition was explored in this study. S125IB was stable at room temperature in dark. Cold SIB was prepared as a standard and IR and NMR results were given in this article.

关键词：放射性同位素示踪 STB 稳定性放射化学

极端洪水导致的外源营养和水位升高对黑藻(Hydrillaverticillata)、附着藻类和水质的影响

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湖泊科学 2025年第1期37卷 184-193,I0018,I0019页

作者：罗东黄文强丁明明黎磊南昌大学鄱阳湖环境与资源利用教育部重点实验室南昌大学生命科学学院南昌330031 江西鄱阳湖湿地保护与恢复国家长期科研基地江西鄱阳湖湿地生态系统国家定位观测研究站南昌330031 鄱阳湖流域生态水文监测研究江西省重点实验室南昌330002

极端洪水显著地影响水生植物的生存环境,带来的连锁效应可能是导致沉水植物大规模衰退的原因之一。极端洪水导致的外源营养、水位升高是影响沉水植物生长和繁殖的重要因素,因此以湖泊代表性沉水植物黑藻(Hydrilla verticillata)为对象,... 详细信息

极端洪水显著地影响水生植物的生存环境,带来的连锁效应可能是导致沉水植物大规模衰退的原因之一。极端洪水导致的外源营养、水位升高是影响沉水植物生长和繁殖的重要因素,因此以湖泊代表性沉水植物黑藻(Hydrilla verticillata)为对象,通过模拟研究探讨极端洪水事件导致的水位波动(水位在短时间内升高)及营养脉冲(营养物质在短时间内增加)对沉水植物、附着藻类和水体理化性质的影响。设置4种水位及营养盐负荷情景:对照(水位保持在75 cm)、水位由75 cm逐步上升到150 cm+氮磷输入、水位由75 cm急剧上升到150 cm+氮磷输入以及水位保持75 cm+氮磷输入,其中后3项处理氮、磷输入量相同,90 d内同步监测了浮游植物、附着藻类以及水体氮、磷浓度的变化。结果表明,对照组中,黑藻雌株相较于雄株个体更大,而极端降水(水位急剧上升)处理下,雄株的相对生长速率(RGR)、根生物量和繁殖器官数均大于雌株,表现出明显的性别二态性。雌性个体对水位波动(水位急剧上升和逐步上升)和营养脉冲的响应比雄性个体响应更为敏感。水位急剧上升和逐步上升均抑制了黑藻根生物量积累、RGR和主枝数,但株高、地上生物量和繁殖生物量保持稳定,水位急剧上升同时抑制了附着藻类的生长。营养脉冲提高了上覆水氮浓度,促进浮游植物和周丛藻类生长,对黑藻地上部分、根和繁殖生物量均有显著抑制作用。实验设置的水位范围内,水位升高对黑藻的地上部分生长及繁殖的影响不明显,而氮、磷输入增加及水位波动的联合作用抑制了其地上部分生物量及繁殖输出,因此营养脉冲增强了水位升高对黑藻的影响效应。由此可以认为,极端洪水事件引起的水位波动叠加营养脉冲的复合效应可能会对沉水植物产生明显的抑制作用,随着未来降水强度和频率增加,水生态系统将受到较大的冲击。

关键词：浅水湖泊沉水植物水位波动富营养化气候变化有性繁殖

Atomic Electronic Contract ProtocolBased on Convertible Signature

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Wuhan University Journal of Natural Sciences 2005年第1期10卷 227-230页

作者： LIUYi-chun wangli-na ZHANGHuan-guo SchoolofComputer WuhanUniversityWuhan430072HubeiChina

A new class of atomicity, namely contract atomicity is presented. A newtechnical strategy based on convertible signature and two-phase commitment is proposed forimplementing atomicity of electronic contract protocol. A new atomic contract signing protocol isgiven out by using ElGam-al-like convertible undeniable signature and commitment of conversion key,and another new atomic contract signing protocol is brought forward by using RSA-based convertibleundeniable signature scheme and commitment ofconversion key. These two new protocols are proved tobe of atomicity, fairness, prnacy- non-repudiation.

关键词： electronic contract atomicity convertible signature

A Heat Shock-Switchable Gene Expression System

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Agricultural Sciences in China 2003年第7期2卷 722-727页

作者： CHENMing wangli-xia ZHONGJin XUHui-jun LINZhong-ping ChineseAcademyofAgriculturalSciences Beijing100081P.R.China StateKeyLaboratoryofProteinEngineeringandPlantGeneticEngineering PekingUniversityBeijing100871P.R.China

The expression of β-D-glucuronidase(Gus)gene under the control of soybean heat shock protein (HSP)promoter Gmhsp17. 5-C was investigated in transgenic Nicotiana plumbaginifolia plants after heat shock treatment. Results of histochemical stain assay showed that the Gus activity increased markedly during the recovery phase(21℃)after heat shock treatment at 42℃ for 2 h. Results of quantitative fluorometric assays indicated that maximal Gus activity after heat shock treatment could reach 5-6 times higher than that before heat shock treatment in transgenic lines HG6 and HG9. Based on several advantages of the heat shock promoter such as easy to induce, sensitivity to heat shock and high inductive activity, it can be potentially used to control expression of gene of interest in transgenic plants.

关键词： Tobacco Promoter Heat shock Inducible

单宁酸改性互穿网络水凝胶促进断裂跟腱术后的组织重塑

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中国组织工程研究 2025年第4期29卷 721-729页

作者：张博张振江东北京大学第三医院运动医学科北京市100191

背景:跟腱断裂术后的再生与重塑是临床治疗的难点,组织工程水凝胶材料为促进跟腱修复提供了可能。目的:探究单宁酸改性互穿网络水凝胶对大鼠断裂跟腱组织再生与重塑的作用。方法:①通过蓝光照射及CaSO4溶液浸泡交联制备单宁酸改性互穿... 详细信息

背景:跟腱断裂术后的再生与重塑是临床治疗的难点,组织工程水凝胶材料为促进跟腱修复提供了可能。目的:探究单宁酸改性互穿网络水凝胶对大鼠断裂跟腱组织再生与重塑的作用。方法:①通过蓝光照射及CaSO4溶液浸泡交联制备单宁酸改性互穿网络水凝胶,表征水凝胶的微观形貌、力学性能、黏附性能、体外释药性能与生物相容性。②取30只SD大鼠,采用随机数字表法分为假手术组、手术组与水凝胶组,每组10只,后2组建立右后肢跟腱断裂模型,手术组采用改良Kessler法将断裂跟腱重新缝合,水凝胶组采用相同术式缝合断裂跟腱后将单宁酸改性互穿网络水凝胶贴片完全缠绕包裹于断端接合处。术后4周,对断裂跟腱进行影像学检查、组织学评价、生物力学测试及炎症因子水平检测。结果与结论:①扫描电镜下可见单宁酸改性互穿网络水凝胶呈多孔微观结构,孔径为3-10μm,并且水凝胶具备良好的体外释药性能、黏附强度与拉伸强度;CCK-8检测与活/死染色显示,该水凝胶对大鼠骨髓间充质干细胞增殖活性无明显影响,具备良好的生物相容性。②MRI成像显示,与手术组比较,水凝胶组大鼠跟腱呈均一低信号,跟腱前后径厚度减小,与周围组织分界更为明确,表现更接近假手术组。苏木精-伊红与Masson染色显示,手术组大鼠跟腱纤维排列松散混乱,细胞密度增加且排布杂乱,伴有明显炎性细胞浸润,部分区域出现腱内骨化;水凝胶组大鼠跟腱纤维排列有序,细胞密度降低且排布规整,炎性细胞浸润明显减少。手术组大鼠跟腱的拉伸强度低于假手术组(P<0.05),水凝胶组大鼠跟腱的拉伸强度高于手术组(P<0.05)。与假手术组比较,手术组大鼠跟腱中白细胞介素1β、白细胞介素6及肿瘤坏死因子α质量浓度及mRNA表达升高(P<0.05);与手术组比较,水凝胶组3种炎症因子水平及mRNA表达降低。③结果表明,单宁酸改性互穿网络水凝胶可显著抑制局部炎症反应、促进断裂跟腱组织重塑。

关键词：单宁酸水凝胶互穿网络跟腱断裂组织重塑组织再生