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Analysis of cytosine methylation in early generations of resynthesized Brassica napus

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Journal of Integrative Agriculture 2016年第6期15卷 1228-1238页

作者： RAN Li-ping FANG Ting-ting RONG Hao JIANG Jin-jin FANG Yu-jie wang you-ping Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University

DNA methylation, an important epigenetic modification, serves as a key function in the polyploidization of numerous crops. In this study, early generations of resynthesized Brassica napus （F1,S1-S3）, ancestral parents B. rapa and B. oleracea were analyzed to characterize their DNA methylation status during polyploidization, applying DNA methylation-sensitive amplifica- tion polymorphism （MSAP） and high-performance liquid chromatography methods. In F, 53.4% fragments were inherited from both A- and C-genomes. Besides, 5.04 and 8.87% fragments in F were inherited from A- and C- genome, respectively. 5.85 and 0.8% fragments were newly appeared and disappeared in resynthesized B. napus, respectively. 13.1% of these gene sites were identified with methylation changes in F, namely, hypermethylation （7.86%） and hypomethylation （5.24%）. The lowest methylation status was detected in F （38.7%） compared with in S1-S3. In S3, 40.32% genes were methylated according to MSAP analysis. Sequencing of methylated fragments indicated that genes involved in multiple biological processes were modified, including transcription factors, protein modification, and transporters. Expression ananlysis of DNA methyltransferase I and DNA methyltransferase chromomethylase 3 in different materials was consistent to the DNA methylation status. These results can generally facilitate dissection of how DNA methylation contributes to genetic stability and improvement of B. napus during polypLoidization.

关键词： resynthesized Brassica napus DNA methylation epigenetics polyploidization

Transcriptional proffling between yellow-and black-seeded Brassica napus reveals molecular modulations on flavonoid and fatty acid content

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Journal of Integrative Agriculture 2022年第8期21卷 2211-2226页

作者： RONG Hao YANG Wen-jing XIE Tao wang Yue wang Xia-qin JIANG Jin-jin wang you-ping Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education of ChinaYangzhou UniversityYangzhou 225009P.R.China

Brassica napus is an important cash crop broadly grown for the vegetable and oil ***-seeded *** is preferred by breeders due to its improved oil and protein quality,less pigments and lignin compared with the blackseeded *** study compared the differences in flavonoid and fatty acid contents between yellow rapeseed from the progenies of ***-Sinapis alba somatic hybrids and the black-seeded counterpart using RNA-seq *** HPLC-PDA-ESI(-)/MSanalysis,it was found that phenylpropanoids and flavonoids(i.e.,isorhamnetin,epicatechin,kaempferol,and other derivatives)in yellow seed were significantly lower than those in black *** fatty acid(FA)content in yellow rapeseed was higher than that in black rapeseed due to the variation of C16:0,C18:0,C18:1,C18:2,and C18:3 ***-seq analysis of seeds at four and five weeks after flowering(WAF)indicated that differentially expressed genes(DEGs)between black and yellow rapeseeds were enriched in flavonoid and FA biosynthesis,including BnTT3,BnTT4,BnTT18,and ***,genes related to FA biosynthesis,desaturation and elongation(FAD3,LEC1,FUS3,and LPAT2)in yellow seed were up-regulated compared to those in black seed,while genes involved in beta-oxidation cycle(AIM1 and KAT2)of yellow seed were down-regulated compared to those in black *** DEGs related to the variation of flavonoids,phenylpropanoids,and FAs would help improve the knowledge of yellow seed character in *** and promote rapeseed improvement.

关键词： rapeseed gene expression analysis fatty acid composition phenolic content yellow seed

Abortive Process of a Novel Rapeseed Cytoplasmic Male Sterility Line Derived from Somatic Hybrids Between Brassica napus and Sinapis alba

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Journal of Integrative Agriculture 2014年第4期13卷 741-748页

作者： wang Juan GAO Ya-nan KONG Yue-qin JIANG Jin-jin LI Ai-min ZHANG Yong-tai wang you-ping Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Yangzhou University Jiangsu Institute of Agricultural Sciences in the Lixiahe District

Somatic hybridization is performed to obtain significant cytoplasmic male sterility （CMS） lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-IA, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-IB, and the abortive process of SaNa-IA were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-IA was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-IA aborted in the pollen mother cell （PMC） stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-IA is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.

关键词： Brassica napus anther abortion cytoplasmic male sterility (CMS) semi-thin sections somatic hybridization

Trehalose and trehalose-hydrolyzing enzyme in the haemolymph of Locusta rnigratoria infected with Metarhizium anisopliae strain CQMa102

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Insect Science 2007年第4期14卷 277-282页

作者： HUA ZHAO ZHONG-KANG wang you-ping YIN YAN-LING LI ZHEN-LUN LI GUO-XIONG PENG YU-XIAN XIA GeneticEngineeringResearchCenter CollegeofBioengineeringChongqingUniversityChongqingChina

Topical application of the Metarhizium anisopliae var. acridum specialist strain CQMa 102 to the locust Locusta migratoria manilensis results in changes of the concentrations of trehalose and glucose in the haemolymph. Micrographs of the locust haemolymph shows Metarhizium anisopliae can effectivly penetrate the external skeleton of locust and after 2 days infection, the hyphae body will appear in the haemolymph of infected insects. The time in decrease of trehalose concentration coincided with that in increase of trehalose-hydrolysing enzyme activity in the haemolymph of the fungus-infected insects. Overlay gel analysis indicated there was considerably more trehalose-hydrolysing activity in the haemolymph of locusts infected by fungus than in controls. A comparable isoform was identified in in vitro culture of the fungus, suggesting a fungal origin for the in vivo enzyme. Haemolymph trehalose decreased significantly during mycosis of locusts by M. anisopliae. All these results suggested that this fungus may take advantage of competing nutrient utilization against the insect by its trehalose-hydrolyzing enzyme secretion. It may provide fundamental knowledge for fungal pathogenesis.

关键词： Metarhizium anisopliae Locusta migratoria manilensis trehalose-hydrolysingenzymes trehalose glucose mycosis

An Efficient System TiCl_4-Al for the Homocoupling Reaction of Aromatic Aldehydes

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Chemical Research in Chinese Universities 2000年第4期16卷 320-323页

作者： LI Ting-you, wang Li-ping, CUI Wei, ZHAO Jian-zhang and wang Zong-mu (College of Life Science, Jilin University, Changchun 130023, P. R. China) 吉林大学吉林长春 130023

The reduction of TiCl4(THF)2 with Al in CH2Cl2 gave a green solution of [Ti2(μ-Cl)2Cl4· (THF)4] (1) which was found to promote the reductive homocoupling of aromatic aldehydes to yield symmetrical 1, 2-diols with hi... 详细信息

关键词： Pinacol coupling reaction Aromatic aldehyde Diastereoselectivity

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吴蕴初与近代中国民族化工工业的兴起

四川师范大学学报（社会科学版） 2008年第1期35卷 118-123页

作者：王友平四川大学历史文化学院成都610064

我国著名的爱国实业家,1930年代上海民族工业骄子吴蕴初研究生产国产味精,并建成"天"字号化工企业集团,填补了中国化学工业的一些空白。抗日战争时期,吴将其"天"字号化工企业内迁四川,一定程度上奠定了四川现代化工企业的基础,并拟在全... 详细信息

我国著名的爱国实业家,1930年代上海民族工业骄子吴蕴初研究生产国产味精,并建成"天"字号化工企业集团,填补了中国化学工业的一些空白。抗日战争时期,吴将其"天"字号化工企业内迁四川,一定程度上奠定了四川现代化工企业的基础,并拟在全国各大地区扩建天原化工厂。新中国成立后,他又回国积极投身祖国化学工业。他重视科技教育,培养、资助了一大批优秀化工科技人才,对近代中国民族化工企业的兴起做出了多方面的重要贡献。

关键词：近代中国吴蕴初天字号化工集团民族工业史

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后革命氛围中的革命历史再叙事

华中师范大学学报（人文社会科学版） 2011年第1期50卷 76-84页

作者：王又平孙斐娟华中师范大学文学院湖北武汉430079

"文革"结束后,随着市场经济的不断推进与政治转型的逐步展开,中国社会处在"后革命氛围"当中。在这一政治文化背景下,20世纪90年代以来的革命历史叙事更加鲜明地呈现出多样的景观,在不同精神层面上展开了对经典革命历史叙事的重写,即革... 详细信息

"文革"结束后,随着市场经济的不断推进与政治转型的逐步展开,中国社会处在"后革命氛围"当中。在这一政治文化背景下,20世纪90年代以来的革命历史叙事更加鲜明地呈现出多样的景观,在不同精神层面上展开了对经典革命历史叙事的重写,即革命历史再叙事。革命历史再叙事纠结在政治、市场、大众、知识分子等多重关系中。本文归纳、阐述了它们所表现出来的几种文化取向和书写方式——续写:文化怀旧中的英雄归来与神话再造;补写:历史疑惑中的思想偏转与话语突围;改写:批判视域中的理想解构与神圣祛魅;戏写:娱乐消费中的狂欢谐谑与传奇重构。当然这几种书写也常常相互纠结。

关键词：革命历史再叙事续写补写改写戏写

西替利嗪与咪唑斯汀治疗慢性荨麻疹的成本效果分析

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中国现代应用药学 2005年第Z2期22卷 770-772页

作者：王幼萍王智康奉化市人民医院浙江奉化315500

目的:对西替利嗪和咪唑斯汀治疗慢性荨麻疹的成本效果进行分析。方法将患者分为两组,每组30例,分别服用西替利嗪和咪唑斯汀,除观察疗效外还进行成本效果分析。结果两组疗效相当,均无明显的不良反应。但西替利嗪的治疗成本明显低于咪唑... 详细信息

目的:对西替利嗪和咪唑斯汀治疗慢性荨麻疹的成本效果进行分析。方法将患者分为两组,每组30例,分别服用西替利嗪和咪唑斯汀,除观察疗效外还进行成本效果分析。结果两组疗效相当,均无明显的不良反应。但西替利嗪的治疗成本明显低于咪唑斯汀。结论西替利嗪和咪唑斯汀治疗慢性荨麻疹疗效高、安全性好。西替利嗪比咪唑斯汀治疗成本低。

关键词：慢性荨麻疹西替利嗪咪唑斯汀成本效果分析

转mEPSPS基因甘蓝型油菜对根际土壤细菌群落结构和多样性的影响

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中国油料作物学报 2021年第1期43卷 131-140页

作者：杜坤王婷杨阳李金萍王幼平扬州大学生物科学与技术学院江苏扬州225009

转基因作物外源基因的表达是否会对土壤微生物生态系统产生不利影响是转基因作物环境安全评价中的重要一环,也关系到作物新品种的应用与推广。为此,本文采用第三代16S扩增子高通量测序技术,对转mEPSPS基因的甘蓝型油菜甲HX6及其亲本甲9... 详细信息

转基因作物外源基因的表达是否会对土壤微生物生态系统产生不利影响是转基因作物环境安全评价中的重要一环,也关系到作物新品种的应用与推广。为此,本文采用第三代16S扩增子高通量测序技术,对转mEPSPS基因的甘蓝型油菜甲HX6及其亲本甲9707不同发育阶段的根际土壤细菌群落进行了比较分析。结果显示,样品中油菜根际的优势细菌主要有变形菌门、拟杆菌门、酸杆菌门、放线菌门等。在同一发育时期,转基因材料和对照根际细菌的Alpha多样性分析(如Shannon、Simpson指数),主坐标(PCoA)分析和MRPP分析均显示无显著差异。此外,还发现转mEPSPS基因油菜在花期根际中浮霉菌门的相对丰度显著低于对照,三个时期中均有少数细菌属的相对丰度也存在显著性差异。不同发育时期,油菜根际细菌的多样性和群落结构差异较大,尤其是苗期与另两个时期相比差异显著。总的来看,转mEPSPS基因甘蓝型油菜的基因转化事件并未对根际土壤的细菌群落产生显著的不利影响。但是,为了更加全面科学地评价其是否存在潜在的土壤微生物生态安全风险,还需要在较长时期内监测其动态变化过程。

关键词： mEPSPS基因甘蓝型油菜微生物多样性群落结构