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Expressions of SE-1, CD31 and CD105 in the vascular endothelial cells and serum of rat with hepatocellular carcinoma

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Chinese Medical Journal 2010年第6期123卷 730-733页

作者： wang jing-yu XU Xiao-yuan JIA jing-hui WU Chi-hong GE Ruo-wen Department of Infectious Disease Peking University First HospitalBeijing 100034China Department of Biological Science National University of SingaporeSingapore 117543Singapore

Background Hepatocellular carcinoma （HCC） is one of the deadliest cancers worldwide. In order to investigate the molecular biologic mechanism of HCC＇s development, we studied the expressions of SE-1, CD105 and CD31 in tumor endothelial cells （TECs） of HCC and in the serum of rats. Methods We analyzed the expressions of SE-1, CD31 and CD105 in rat HCC tumor tissues using immunohistochemistry （IHC）. Twenty HCC bearing rats and eighteen normal rats were examined for the expressions of SE-1, CD31 and CD105 antigens in serum by enzyme-linked immunosorbent assay （ELISA）. Results SE-1, CD31 and CD105 antigens were detected both in HCC tissue and in normal liver tissue with higher expressions of CD31 and CD105 in HCC while the SE-1 antigen expression was higher in normal liver. Similarly, serum CD31 and CD105 in rats with HCC were significantly increased compared with normal rats （t=-2.8628, P=0.0086; t=4.4922, P 〈0.0001, respectively）. In contrast, SE-1 antigen in HCC rat serum was significantly decreased compared with normal rats （t=3.4983, P=-0.0011）.Conclusion SE-1, CD31 and CD105 are closely related with liver tumor angiogenesis, which is similar to their performances in terms of their expressions in the serum.

关键词： hepatocellular carcinoma tumor angiogenesis endothelial cell immunohistochemistry enzyme-linked immunosorbent assay

Expression of PIN Genes in Rice （Oryza sativa L.）： Tissue Specificity and Regulation by Hormones

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Molecular Plant 2009年第4期2卷 823-831页

作者： Ji-Rong wang Han Hu Gao-Hang wang jing Li Jie-yu Chen Ping Wu State Key Laboratory of Plant Physiology and Biochemistry College of Life Sciences Zi Jin Gang Campus Zhejiang University Hangzhou 310058 People's Republic of China

Twelve genes of the PIN family in rice were analyzed for gene and protein structures and an evolutionary relationship with reported AtPINs in Arabidopsis. Four members of PIN1 （designated as OsPINla-d）, one gene paired with AtPIN2 （OsPIN2）, three members of PIN5 （OsPIN5a-c）, one gene paired with AtPIN8 （OsPIN8）, and three monocot-specific PINs （OsPIN9, OsPINIOa, and b） were identified from the phylogenetic analysis. Tissue-specific expression patterns of nine PIN genes among them were investigated using RT-PCR and GUS reporter. The wide variations in the expression domain in different tissues of the PIN genes were observed. In general, PIN genes are up-regulated by exogenous auxin, while different responses of different PIN genes to other hormones were found.

关键词： Oryza sativa L. PIN family expression pattern.

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu Jie Mu yu Leng Lei Ma yu Zheng Ruizhi Li Yin wang Peifeng Li Hai Zhu Dong wang jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

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电力系统非线性振荡模态分析

电力系统保护与控制 2010年第20期38卷 1-5,11页

作者：王宇静于继来哈尔滨工业大学电气工程系黑龙江哈尔滨150001

利用Carleman线性化原理研究电力系统非线性振荡稳定性问题,通过分析得到了二阶及二阶以上电力系统动态方程解析解的表达式。通过Carleman线性化分析方法得到了系统的非线性高阶模态,可以用于研究电力系统的非线性动态特性及大干扰下系... 详细信息

利用Carleman线性化原理研究电力系统非线性振荡稳定性问题,通过分析得到了二阶及二阶以上电力系统动态方程解析解的表达式。通过Carleman线性化分析方法得到了系统的非线性高阶模态,可以用于研究电力系统的非线性动态特性及大干扰下系统的稳定性,揭示了非线性模态相关性对系统动态特性的影响。同时将线性模态参与因子的概念扩展到非线性模态中,定量地衡量各振荡模式之间的非线性相关作用。通过36节点系统的仿真计算与Prony分析结果进行了对比。通过Carleman线性化方法分析电力系统非线性模式之间的相互作用,可以在小干扰稳定和传统的线性化分析基础上更加深入地理解非线性系统的动态特性,为分析大干扰和强非线性情况下系统的稳定性和动态特性提供了一种新的手段。

关键词：电力系统低频振荡 Carleman线性化非线性振荡非线性相关

Wild-Type KRAS and BRAF Could Predict Response to Cetuximab in Chinese Colorectal Cancer Patients

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Chinese Journal of Cancer Research 2011年第4期23卷 271-275页

作者： jing Gao, Ting-ting wang, jing-wei yu, Yan-yan Li, Lin Shen* Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Gastrointestinal Oncology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Gastrointestinal Oncology Peking University School of Oncology Beijing Cancer Hospital & Institute Beijing 100142 China.

Objective: To analyze the relationship between KRAS, BRAF mutations and the response to Cetuximab in Chinese colorectal cancer patients. Methods: A total of 273 Chinese colorectal cancer patients were evaluated for KRAS and BRAF mutations by Sanger sequencing. Among them, 59 patients with metastatic colorectal cancer (mCRC) were treated with Cetuximab in combination with chemotherapy from August 2005 to July 2009. Statistical analysis was conducted to assess the relationship between KRAS, BRAF mutations and the response or survival of 59 mCRC patients. Results: KRAS and BRAF mutation rates were 38.5% (105/273) and 5.1% (14/273), respectively, and KRAS/BRAF mutations were mutually exclusive. Among 59 patients treated with Cetuximab plus chemotherapy, KRAS and BRAF mutations were identified in 11 and 5 patients, respectively. The response rates and median progression-free survivals (PFS) in KRAS wild-type and mutant patients were 35.4% (17/48) vs. 9.1% (1/11) (P=0.054) and 153 days vs. 99 days (P=0.01), respectively. Also, the response rates and median PFS in BRAF wild-type and mutant patients were 37.2% (16/43) vs. 20% (1/5) (P=0.016) and 138 days vs. 90 days (P=0.036), respectively. Conclusion: Besides KRAS, assessing BRAF mutation should also be required to select patients eligible for Cetuximab. Further prospective evaluation in large samples should be performed to confirm these preliminary findings.

关键词： KRAS BRAF Mutation Cetuximab Colorectal cancer

FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression

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Neural Regeneration Research 2025年第7期20卷 2068-2083页

作者： jing Yao yuan Li Xi Liu Wenping Liang yu Li Liyong Wu Zhe wang Weihong Song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Pediatrics The Second Affiliated Hospital and Yuying Children’s HospitalKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine Visionand Brain Health)WenzhouZhejiang ProvinceChina

Alzheimer's disease is characterized by deposition of amyloid-β,which forms extracellular neuritic plaques,and accumulation of hyperphosphorylated tau,which aggregates to form intraneuronal neurofibrillary tangles,in the *** NLRP3 inflammasome may play a role in the transition from amyloid-βdeposition to tau phosphorylation and *** NLRP3 is primarily found in brain microglia,and tau is predominantly located in neurons,it has been suggested that NLRP3 expressed by microglia indirectly triggers tau phosphorylation by upregulating the expression of pro-inflammatory ***,we found that neurons also express NLRP3 in vitro and in vivo,and that neuronal NLRP3 regulates tau *** biochemical methods,we mapped the minimal NLRP3 promoter and identified FUBP3 as a transcription factor regulating NLRP3 expression in *** primary neurons and the neuroblastoma cell line Neuro2A,FUBP3 is required for endogenous NLRP3 expression and tau phosphorylation only when amyloid-βis *** the brains of aged wild-type mice and a mouse model of Alzheimer's disease,FUBP3 expression was markedly increased in cortical *** analysis suggested that FUBP3 plays a role in neuron-mediated immune *** also found that FUBP3 trimmed the 5′end of DNA fragments that it bound,implying that FUBP3 functions in stress-induced *** findings suggest that neuronal NLRP3 may be more directly involved in the amyloid-β-to–phospho-tau transition than microglial NLRP3,and that amyloid-βfundamentally alters the regulatory mechanism of NLRP3 expression in *** that FUBP3 was only expressed at low levels in young wild-type mice and was strongly upregulated in the brains of aged mice and Alzheimer's disease mice,FUBP3 could be a safe therapeutic target for preventing Alzheimer's disease progression.

关键词： 5′end trimming Alzheimer's disease amyloid-beta amyloid-β-dependent transcription FUBP3 inflammasome inflammation neuron NLRP3 tau transcription factor

NEPHTIXAMIDE A AND B FROM THE SOFT CORAL NEPHTHEA TIXIERAE VERSEVELDT

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Chinese Chemical Letters 1993年第6期4卷 499-500页

作者： Gui Yong Sheng wang jing yu SU Long Mei ZENG Department of Chemistry,ZhongshanUniversity,Guangzhou,510275

Two new ceramides,Nephtixamide A and B,were isolated from the soft coral Nephthea tixierae *** structures were established by spectroscopic methods

关键词： OH DEPT NEPHTIXAMIDE A AND B FROM THE SOFT CORAL NEPHTHEA TIXIERAE VERSEVELDT

Links between allergic rhinitis and asthma

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Chinese Medical Journal 2006年第8期119卷 676-683页

作者： LI jing wang Hong-yu ZHANG Chun-qing SUN Bao-qing ZHONG Nan-shan Guangzhou Institute of Respiratory Disease First Affiliated Hospital of Guangzhou Medical School Guangzhou 510120 China

AIlergic diseases of the airway, which include seasonal rhinitis, chronic perennial rhinitis and asthma, are recognized as inflammatory disorders of the airway mucosa, but differ in the location of the inflammatory reaction and clinical manifestations of the disease. Asthma and allergic rhinitis frequently coexist in the same patient and are thought to share common predisposing genetic factors which interact with the environmental influences. Both diseases have increased in prevalence over recent decades particularly in westernized countries. This increase has been largely attributed to environmental factors such as exposure to aerial pollutants and early life events, including the degree of exposure to infectious agents which might affect IgE production, since there has been insufficient time for a significant change in the gene pool.

关键词： allergic rhinitis asthma pathogenesis

菲涅耳波带板应用于聚变靶的高分辨X射线成像分析

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物理学报 2011年第2期60卷 489-495页

作者：王晓方王晶宇中国科学技术大学近代物理系中国科学院基础等离子体物理重点实验室合肥230026

在惯性约束核聚变研究中,为了实现1μm高空间分辨keV-X射线成像,文中发展了菲涅耳波带板(FZP)直接成像的分析方法,并通过数值计算研究了FZP的成像特性.针对钛Kα线(光子能量4.51keV,波长0.275nm),提出了FZP参数,对制作技术的要求较低.... 详细信息

在惯性约束核聚变研究中,为了实现1μm高空间分辨keV-X射线成像,文中发展了菲涅耳波带板(FZP)直接成像的分析方法,并通过数值计算研究了FZP的成像特性.针对钛Kα线(光子能量4.51keV,波长0.275nm),提出了FZP参数,对制作技术的要求较低.研究了靶尺度的影响.FZP的有效视场使它能够对数毫米大尺度靶实现高分辨成像.还研究了入射光的光谱带宽对成像的影响.FZP的色差有助于单色成像,但是带宽超过限度会导致像的反衬度降低.这些结果表明FZP应用于聚变点火靶的高空间分辨X射线成像的能力,也为应用提出了要求.

关键词： X射线成像惯性约束核聚变菲涅耳波带板