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Molecular Characterization of Full-length Genome of Japanese Encephalitis Virus Genotype V Isolated from Tibet, China

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Biomedical and Environmental Sciences 2014年第4期27卷 231-239页

作者： LI Ming Hua FU Shi Hong CHEN Wei Xin wang huan yu CAO yu Xi LIANG Guo Dong State Key Laboratory for Infectious Disease Prevention and Control Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease

Objective To determine the molecular characterization of full-length genome of Japanese encephalitis virus （JEV） genotype V. Methods The full-length nucleotide sequences of JEV strains isolated from different locations and sources were used in sequence and phylogenetic analysis. Results The full-length genome of genotypes V JEV, XZ0934, and Muar strain were composed of 10 983 and 10 988 nucleotides respectively and shared a lower level of identity with JEV genotypes I-IV, ranging from 78.4% （G I, KV1899） to 79.7% （G III, JaGAr01）, for the nucleotide sequences, and from 90.0%（G I, KV1899） to 91.8%（G III, JaGAr01） for the amino acid sequences. The open reading frame （ORF） of JEV genotype V spanned nucleotides 96 to 10 397 and encoded 3 433 amino acids. Interestingly, a comparison with JEV genotype I-IV revealed that 3 nucleotides （encoded with a serine residue） were inserted in the NS4A gene of JEV genotype V, and the insertion of nucleotides was also found in downstream of the ORF stop codon in 3’-untranslated region. Moreover, numerous amino acid mutations were observed in 3 functional domains of the E gene of JEV genotype V. Conclusion The molecular characterization of JEV genotype V is significantly different from that of the known genotypes I-IV. The mutations located in the coding region and the non-coding region may be molecular markers of JEV genotype V and warrant further studies to determine their effects on biology and immunogenicity of genotype V strains.

关键词： Japanese encephalitis virus Genotype V Molecular characterization

The Relationship between Japanese Encephalitis and Environmental Factors in China Explored Using National Surveillance Data

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Biomedical and Environmental Sciences 2018年第3期31卷 227-232页

作者： huanG Xiao Xia YAN Lei GAO Xiao Yan REN yu huan FU Shi Hong CAO yu Xi HE Ying LEI Wen Wen LIANG Guo Dong wang Shi Wen wang huan yu Office for Disease Control and Prevention National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention Harmacoepidemiology Asia-Pacific unit MSD R&D(China) Co.Ltd. Department of Viral Encephalitis National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention Institute of Remote Sensing and Digital Earth Chinese Academy of Sciences

Japanese encephalitis（JE）is a serious public health *** study was undertaken to better understand the relationship between JE distribution and environmental factors in *** data from 2005 to 2010 were retrieved from ... 详细信息

关键词： Japanese encephalitis Environmental factors Landscape

Analysis of Discharge Spark Energy in Buck Converter of a Continuous Mode of Inductive Current

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Journal of China University of Mining and Technology 2006年第4期16卷 514-517,522页

作者： CUI Bao-chun CHENG Hong wang Cong LU huan-yu SHI yun Department of Information and Electrical Engineering China University of Mining ＆ Technology Beijing 100083 China

The basic idea of intrinsically safe circuit and the discharge spark in the Buck converter in the explosive at- mospheres were introduced. The Buck converter is the main topological structure of the switch type of intrinsically safe circuit, which has two working modes: continuous inductive current (CCM — continuous conduction mode) and dis- crete inductance current (DCM — discontinuous conduction mode). The operating state of the continuous inductive current mode is analyzed in detail and the energy of discharge spark in various operating modes is discussed. The total energy will decrease with the increase of switch frequency, in a switching cycle; the discharge spark energy has a maxi- mum and a minimum value. Therefore, the Buck converter has smaller discharge spark energy than the linear power circuit and the switch type of intrinsically safe circuit can enhance the output power and the conversion efficiency of the intrinsically safe power.

关键词： Buck converter discharge spark intrinsically safe

Lignans with(N,N-diethyl)methyl amino group from Buxus rugulosa

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Chinese Journal of Natural Medicines 2021年第9期19卷 675-679页

作者： wang yu-huan YIN Yong CUI Le-Tian LI Qiu-Rong KONG Ling-Yi LUO Jun Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 210009China

Buxrugulosides A–E,four lignan glycosides(1–4)and a protocatechuate derivative(5)featuring a rare(N,Ndiethyl)methyl amino group at aromatic rings,were obtained from the aerial parts of Buxus rugulosa,which is famous for treating coronary heart *** structures including absolute configurations were elucidated by HRMS,1 D and 2 D NMR,and by comparing their CD data with previous *** 1 was a rare sesquilignan,and all of these compounds were the first example of lignans with(N,N-diethyl)methyl amino group.

关键词： Lignan glycoside (N,N-diethyl)methyl amino group Buxus rugulosa

TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus

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Biomedical and Environmental Sciences 2018年第3期31卷 208-214页

作者： SHAO Nan LI Fan NIE Kai FU Shi Hong ZHANG Wei Jia HE Ying LEI Wen Wen wang Qian Ying LIANG Guo Dong CAO yu Xi wang huan yu Department of Viral Encephalitis National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China State Key Laboratory of Infectious Disease Prevention and Control Beijing 102206 China State key laboratory for genetic engineering and molecular virology National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206China School of Public Health Shandong University Jinan 250012 Shandong China Office of laboratory management National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To detect Japanese encephalitis virus（JEV） rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction（RT-PCR） detection system was *** By aligning the full-length sequences of JEV（G1-G5）, six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR *** With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/μL. The coefficients of variation of this real-time RT-PCR were all 〈 2.8%. The amplification efficiency of this method was between 90% and 103%.Conclusion A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes.

关键词： Japanese encephalitis virus Genotype TaqMan real-time RT-PCR

Development of an Internally Controlled Reverse Transcription Recombinase-aided Amplification Assay for the Rapid and Visual Detection of West Nile Virus

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Biomedical and Environmental Sciences 2019年第12期32卷 926-929页

作者： FAN Guo Hao SHEN Xin Xin LI Fan LI Xin Na BAI Xue Ding ZHANG Rui Qing wang Rui huan LEI Wen Wen wang huan yu MA Xue Jun WU Gui Zhen Department of Core Facility Ministry of Health Key Laboratory of Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Biosafety 3 Laboratory Chinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory of Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China Department of Viral Encephalitis NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan 430071China

West Nile virus(WNV)causes West Nile fever and West Nile *** infection by WNV creates serious public health problems,its simple,rapid,and visual detection is very important in clinical practice,especially in resource-limited *** have developed a rapid,specific,and highly sensitive internally controlled reverse transcription recombinase-aided amplification(RTRAA)assay to detect WNV,using both real-timefluoresce nee and the lateral flow dipstick(LFD)at39.0°C for 30 *** analytical sensitivity of theRT-RAA assay was 10 plasmid copies and 1.6 pfu perreacti on with real-time fluoresce nee,and 1,000plasmid copies per reaction with the *** crossreactionwith other control viruses was *** with the RT-qPCR assay,the RT-RAA assaydemonstrated 100%sensitivity and 100%specificityfor WNV.

关键词： Internal Visual aided

Real-time RT-PCR Assay for the Detection of Culex flavivirus

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Biomedical and Environmental Sciences 2015年第12期28卷 917-919页

作者： CAO yu Xi HE Xiao Xia FU Shi Hong HE Ying LI Hao GAO Xiao Yan LIANG Guo Dong wang huan yu State Key Laboratory of Infectious Disease Prevention and Control(SKLID) Department of Viral EncephalitisNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

Based on the Culex flavivirus (CxFV) E gene sequences in GenBank, CxFV-specific primers and probes were designed for real-time reverse transcription-polymerase chain reaction (RT-qPCR). The specificity test revealed that CxFV could be detected using RT-qPCR with the specific CxFV primers and probes; other species of arboviruses were not detected. The stability test demonstrated a coefficient of variation of <1.5%. A quantitative standard curve for CxFV RT-qPCR was established. Quantitative standard curve analysis revealed that the lower detection limit of the RT-qPCR system is 100 copies/mu L. Moreover, RT-qPCR was used to detect CxFV viral RNA in mosquito pool samples. In conclusion, we established a real-time RT-PCR assay for CxFV detection, and this assay is more sensitive and efficient than general RT-PCR. This technology may be used to monitor changes in the environmental virus levels.

关键词： PCR Real-time RT-PCR Assay for the Detection of Culex flavivirus RT time

Syntheses of 3－Methyl－1－propyl－4－piperidoneand1－Methyl－3，7－dipropyl－3，7－diazabicyclo［3，3，1］nonan－9－one

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Chemical Research in Chinese Universities 1994年第3期10卷 266-271页

作者： wang yu-huan , wang Guo-jia, ZHANG Wei-ge, DU BO, FANG BO-yanyu Gui and CHENG yue-mei(Department of Chemistry, Jilin University, Changchun, 130023)

Ｓｙｎｔｈｅｓｅｓｏｆ３－Ｍｅｔｈｙｌ－１－ｐｒｏｐｙｌ－４－ｐｉｐｅｒｉｄｏｎｅａｎｄ１－Ｍｅｔｈｙｌ－３，７－ｄｉｐｒｏｐｙｌ－３，７－ｄｉａｚａｂｉｃｙｃｌｏ［３，３，１］ｎｏｎａｎ－９－ｏｎｅＷＡＮＧＹｕ－ｈ... 详细信息

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关键词： Methyl- 1-propyl-4-piperidone 1-Methyl-3,7-dipropyl-3,7-diazabi-cyclo[3 3 1]nonan-9-one Synthesis Catalysis

Real-time RT-PCR Assay for the detection of Tahyna Virus

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Biomedical and Environmental Sciences 2015年第5期28卷 374-377页

作者： LI Hao CAO yu Xi HE Xiao Xia FU Shi Hong Lyu Zhi HE Ying GAO Xiao Yan LIANG Guo Dong wang huan yu National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

A real-time RT-PCR （RT-qPCR） assay for the detection of Tahyna virus was developed to monitor Tahyna virus infection in field-collected vector mosquito samples. The targets selected for the assay were S segment sequences encoding the nucleocapsid protein from the Tahyna virus. Primers and probes were selected in conserved regions by aligning genetic sequences from various Tahyna virus strains available from GenBank. The sensitivity of the RT-qPCR approach was compared to that of a standard plaque assay in BHK cells. RT-qPCR assay can detect 4.8 PFU of titrated Tahyna virus. Assay specificities were determined by testing a battery of arboviruses, including representative strains of Tahyna virus and other arthropod-borne viruses from China. Seven strains of Tahyna virus were confirmed as positive; the other seven species of arboviruses could not be detected by RT-qPCR. Additionally, the assay was used to detect Tahyna viral RNA in pooled mosquito samples. The RT-qPCR assay detected Tahyna virus in a sensitive, specific, and rapid manner; these findings support the use of the assay in viral surveillance.

关键词： PCR Real-time RT-PCR Assay for the detection of Tahyna Virus time RT