检索结果-南通市图书馆

TEAD1转录本的鉴定及其在鸡前脂肪细胞中的功能研究

在线全文

学校读者我要写书评

暂无评论

生物化学与生物物理进展 2024年第1期51卷 215-229页

作者：彭敏徐虎贾子秋杨清竹潘林王维禹孔令喆孙婴宁齐齐哈尔大学生命科学与农林学院生物技术系齐齐哈尔161006

目的 TEAD1转录因子1 (TEAD1)在前脂肪细胞中表达,但是功能还不清楚。本研究旨在探讨TEAD1的两个转录本对永生化鸡前脂肪细胞系(immortalized chicken preadipocyte 1,ICP1)细胞增殖、迁移、凋亡和分化的影响。方法克隆TEAD1基因的全... 详细信息

目的 TEAD1转录因子1 (TEAD1)在前脂肪细胞中表达,但是功能还不清楚。本研究旨在探讨TEAD1的两个转录本对永生化鸡前脂肪细胞系(immortalized chicken preadipocyte 1,ICP1)细胞增殖、迁移、凋亡和分化的影响。方法克隆TEAD1基因的全长序列,对获得的两个转录本进行生物信息学分析。利用间接免疫荧光分析TEAD1转录本的亚细胞定位。通过RT-qPCR、CCK-8和EdU等方法,检测过表达TEAD1转录本对ICP1细胞增殖的影响。利用划痕实验检测TEAD1转录本对ICP1细胞迁移的影响。利用细胞凋亡-Hoechst染色和RT-qPCR,分析过表达TEAD1转录本对ICP1细胞凋亡的影响。通过RT-qPCR检测TEAD1转录本在不同组织、不同细胞系和ICP1细胞分化过程中的表达。利用油红O染色、BODIPY染色、RT-qPCR、Western blot和双荧光素酶报告基因技术,分析过表达TEAD1转录本对ICP1细胞脂滴积累及成脂相关基因转录的影响。最后,测定过表达TEAD1转录本的ICP1细胞中甘油三酯(TG)含量。结果克隆TEAD1全长编码区,鉴定出两个TEAD1转录本。TEAD1-V1主要定位于细胞核,TEAD1-V2定位于细胞质与细胞核。过表达TEAD1-V1和TEAD1-V2均抑制ICP1细胞增殖。过表达TEAD1-V1促进ICP1细胞迁移,而过表达TEAD1-V2对ICP1细胞迁移没有影响。且过表达TEAD1-V1和TEAD1-V2均促进ICP1细胞凋亡。两个转录本在不同组织和细胞系中的表达模式相似,在前脂肪细胞分化过程中的表达先下降后上升。过表达TEAD1-V1能显著减少ICP1细胞中脂滴的积累(P0.05)。过表达TEAD1-V1能显著降低ICP1细胞中甘油三脂的含量(P0.05)。结论本研究首次克隆并鉴定了鸡TEAD1基因的两个转录本。过表达转录本TEAD1-V1和TEAD1-V2抑制鸡前脂肪细胞增殖并且促进鸡前脂肪细胞凋亡;TEAD1-V1抑制前脂肪细胞分化并促进前脂肪细胞迁移,而TEAD1-V2对前脂肪细胞分化和迁移没有影响。

关键词： TEAD转录因子1 鸡转录本 ICP1

在线全文

学校读者我要写书评

暂无评论

饱和多孔弹性深埋复合式衬砌抗震性能分析

公路交通科技 2024年第3期41卷 132-142页

作者：王观阳陈常科熊文勇邓翔浩丁海滨江西省交通工程集团有限公司江西南昌330025 华东交通大学土木建筑学院江西南昌330013

目前国内外学者针对地震波扰动下隧道的衬砌动力响应解析解的研究多为单层衬砌,而实际工况中隧道一般包括初衬和二次衬砌。因此,研究复合式衬砌隧道的抗震性至关重要。以深埋复合式衬砌隧道为研究对象,建立了考虑尺寸效应和非均匀性结... 详细信息

目前国内外学者针对地震波扰动下隧道的衬砌动力响应解析解的研究多为单层衬砌,而实际工况中隧道一般包括初衬和二次衬砌。因此,研究复合式衬砌隧道的抗震性至关重要。以深埋复合式衬砌隧道为研究对象,建立了考虑尺寸效应和非均匀性结构效应的高阶应变梯度非局部孔隙弹性计算模型,用以描述多孔介质材料和结构的具体物理特性。通过引入2个长度参数、非局部参数及尺度因子来改进Biot理论。采用波函数展开法得到了复合式衬砌的解析解。考虑深埋复合式衬砌隧道的边界条件,确定复合式衬砌隧道周围的动力集中因子大小,分析了不同入射P波频率下,尺寸因子和非局部参数对衬砌动应力集中因子大小的影响,以及内外衬砌刚度和厚度对衬砌内外边界动应力集中因子(DSCF)值的影响。结果表明:高频下,非局部参数和尺寸因子对衬砌内DSCF值的影响较大,并且非局部参数对衬砌内DSCF值有削弱的影响,尺寸因子对衬砌内DSCF值有增强的影响;低频下外衬砌刚度越大,外衬砌内DSCF值越大,内衬砌内DSCF值越小,这说明在外层衬砌不被破坏的情况下,内层衬砌适当选择刚度小一点的材料有利于隧道衬砌的安全;低频下,衬砌厚度在合理范围内越大,衬砌内DSCF值越小。解析结果可为隧道复合衬砌设计提供参考。

关键词：隧道工程应变梯度非局部Biot理论波函数展开法复合式衬砌隧道衬砌刚度

Electroless Plating of Ni Nanoparticles on WC to Assist Its Pressureless Sintering of WC-Ni Cemented Carbide with Enhanced Mechanical and Corrosion-resistant Performance

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2021年第6期36卷 786-795页

作者： min Fanlu YANG Hao YAO Zhanhu LI Xinggao ZHANG Jianfeng LIU hai Key Laboratory of Geomechanics and Embankment Engineering Hohai UniversityMinistry of EducationNanjing 210098China CCCC Tunnel Engineering Company Limited Nanjing 210098China Key Laboratory of Urban Underground Engineering of ministry of Education Beijing Jiaotong UniversityBeijing 100044China College of Mechanics and Materials Hohai UniversityNanjing 211198China

Ni nanoparticles were coated uniformly on the surface of WC powder via a facile electroless plating method(abbreviated as WCN-EP),and then consolidated for mechanical and corrosion resistance performance characterization,in comparison with hand mixed WC-Ni(WCN-H).Under the optimized electroless plating parameters,Ni particles,less than 1μm in average diameter,were found to be uniformly and densely wrapped on the surface of the tungsten carbide matrix of *** comparison,in WCN-H,the Ni particles about 1.8μm in average diameter,were randomly distributed together with irregular WC *** uniform coating of Ni was found to assist the densification process of WCN-EP effectively,with higher densities and less pores than those of WCN-H at the Ni content of 10.6wt%,25.5wt%,and 30.3 wt%.However,at the Ni content of 18.8wt%,the relative densities of WCN-EP and WCN-H both increased to the maximum value of 98%.The maximum hardness of the consolidated WCN-EP was 82.6 HRA,about 1.2 HRA higher than that of *** addition,the consolidated WCN-EP also exhibits a superior corrosion resistance by the polarization curve analysis at an electrochemical workstation.

关键词： electroless plating tungsten carbide(WC) WC-Ni preparation process corrosion-resistant performance

Gibberellin Signal Transduction in Rice

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2007年第6期49卷 731-741页

作者： Liu-min Fan Xiaoyan Feng Yu wang Xing wang Deng Peking-Yale Joint Center of Plant Molecular Genetics and Agro-Biotechnology National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China Department of Molecular Cellular and Developmental Biology Yale University New Haven Connecticut 06520-8104 USA

In the past decade, significant knowledge has accumulated regarding gibberellin （GA） signal transductlon In rice as a result of studies using multiple approaches, particularly molecular genetics. The present review highlights the recent developments In the identification of GA signaling pathway components, the discovery of GA-Induced destructlon of GA signaling repressor （DELLA protein）, and the possible mechanism underlying the regulation of GA- responsive gene expression in rice.

关键词： gene expression gibberellin protein degradation rice signal transduction.

One-dimensional and two-dimensional nanomaterials for the detection of multiple biomolecules

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2019年第9期30卷 1557-1564页

作者： Quan wang Xudong wang min Xu Xiaoding Lou Fan Xia Engineering Research Center of Nano-Geomaterials of ministry of Education Faculty of Materials Science and Chemistry China University of Geosciences Wuhan 430074 China Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica School of Chemistry and Chemical Engineering Huazhong University of Science and Technology Wuhan 430074 China Zhejiang Institute China University of Geosciences Wuhan Hangzhou 311305 China

The complexity of biological samples determines that the detection of a single biomolecule is unable to satisfy actual needs. Moreover, the "false positives" results caused by a single biomolecule detections easily leads to erroneous clinical diagnosis and treatment. Thus, it is important for the homogenous quantification of multiple biomolecules in not only basic research but also practical application. As a consequent, a large number of literatures have been exploited to monitor multiple biomolecules in homogenous solution, enabling facilitating the development of the disease diagnosis, treatment as well as drug discovery. One-dimensional nanomaterials and two-dimensional nanomaterials have special physical and chemical properties, such as good electrochemical properties, stable structure, large specific surface area, and biocompatibility, which are widely used in electrochemical and fluorescent detection of biomolecules. This tutorial review highlights the recent development for the detection of multiple biomolecules by using nanomaterials including one-dimensional materials(1DMs) as well as twodimensional materials(2DMs).

关键词： One-dimensional nanomaterials Two-dimensional nanomaterials Detection Multiple targets Biomolecules

Effect of drying environment on engineering properties of an expansive soil and its microstructure

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2017年第6期14卷 1194-1201页

作者： KONG Ling-wei wang min GUO Ai-guo wang Yong State Key Laboratory of G-eomechanics and G-eotechnical Engineering Institute of Rock and Soil Mechanics Chinese Academy of Sciences Wuhan 430071 China Rockfield Software Limited Swansea Wales SA1 8AS UK

This paper investigates the effect of drying environment, i.e. temperature and relative humidity, on the engineering properties and microscopic pore size distribution of an expansive soil. The shrinkage tests under different drying temperatures and relative humidity are carried out in a constant climate chamber. Then, the undisturbed samples, prepared in different drying environment, are used for the triaxial tests and mercury intrusion tests. It is found that the drying environment has noticeable influence on the engineering properties of expansive soils and it can be characterized by the drying rate. The linear shrinkage and strength increase with the decrease of the drying rate. The non-uniform deformation tends to happen in the high drying rate, which subsequently furthers the development of cracks. In addition, during the drying process, the variation of pores mainly focuses on the inter-aggregate pores and inter-particle pores. The lower drying rate leads to larger variation of pore size distribution.

关键词： Expansive soils Drying environment Shrinkage Strength Pore size distribution Triaxial test

宫颈脱落细胞特殊染色技术在宫颈病变筛查中的应用价值

在线全文

学校读者我要写书评

暂无评论

中国计划生育学杂志 2024年第10期32卷 2340-2344页

作者：王敏李敏王宁刘玉珍曹成成张海凤山东第二医科大学临床医学院维坊261042 山东第二医科大学附属医院国家卫生健康委科学技术研究所山东省潍坊市人民医院

目的:探讨宫颈脱落细胞特殊染色技术在宫颈病变筛查中的临床应用价值。方法:选取2022年8月-2023年5月在山东第二医科大学附属医院及潍坊市人民医院进行宫颈病变筛查的女性共2000例,所有对象均进行宫颈脱落细胞特殊染色技术(FRD)检测、... 详细信息

目的:探讨宫颈脱落细胞特殊染色技术在宫颈病变筛查中的临床应用价值。方法:选取2022年8月-2023年5月在山东第二医科大学附属医院及潍坊市人民医院进行宫颈病变筛查的女性共2000例,所有对象均进行宫颈脱落细胞特殊染色技术(FRD)检测、人乳头瘤病毒(HPV)和液基薄层细胞(TCT)检测。3种筛查方法任一结果阳性病例行阴道镜检查,阴道镜下有病变者行宫颈活检。以宫颈组织病理结果为金标准,比较FRD、TCT、HPV、HPV+TCT组的检测效能及受试者工作特征曲线下面积(AUC)。结果:FRD检测灵敏度为83.8%、特异度为90.0%,Kappa值为0.618,AUC为0.869;TCT检测的灵敏度为65.1%、特异度为89.5%,Kappa值为0.485,AUC为0.773;HPV检测的灵敏度为94.6%、特异度为71.7%,Kappa值为0.387,AUC为0.832;HPV+TCT联合检测的灵敏度为98.9%、特异度为70.2%,Kappa值为0.391,AUC为0.846。结论:宫颈脱落细胞特殊染色试验用于宫颈病变筛查有较好的效果,可用于基层宫颈病变筛查。

关键词：宫颈癌筛查宫颈脱落细胞特殊染色技术液基薄层细胞检测人乳头瘤病毒检测

在线全文

学校读者我要写书评

暂无评论

精梳机分离罗拉齿轮传动机构优化

纺织学报 2024年第3期45卷 177-184页

作者：刘金儒李新荣王建坤王浩师帅星王彪天津工业大学机械工程学院天津300387 天津市现代机电装备技术重点实验室天津300387 天津工业大学纺织科学与工程学院天津300387

混合驱动的精梳机分离罗拉齿轮传动机构的动力学性能直接影响车速的提高,为改善传动机构的动力学性能,首先,分析现有混合驱动的精梳机分离罗拉齿轮传动机构,采用集中质量法建立传动机构的固有频率模型,并计算传动机构在不同精梳机车速... 详细信息

混合驱动的精梳机分离罗拉齿轮传动机构的动力学性能直接影响车速的提高,为改善传动机构的动力学性能,首先,分析现有混合驱动的精梳机分离罗拉齿轮传动机构,采用集中质量法建立传动机构的固有频率模型,并计算传动机构在不同精梳机车速下的激励频率,发现当车速为600钳次/min时机构会出现共振;然后,采用导数法建立传动机构固有频率对机构中各个构件转动惯量的灵敏度模型,发现行星轮p的转动惯量对一阶固有频率的影响较大;最后,通过优化传动机构中WW型差动轮系的各个齿轮齿数以改变其转动惯量,使传动机构的一阶固有频率比原先提高了43.44%,避免了机构在600钳次/min时出现共振。

关键词：精梳机分离罗拉混合驱动齿轮传动机构

Discovery of Paleogene palynological assemblages from the Wanbaogou Group-complex in western part of the Eastern Kunlun orogenic belt and its geological significance

在线全文

学校读者我要写书评

暂无评论

Science China Earth Sciences 2006年第4期49卷 358-367页

作者： GUO Xianpu wang Naiwen DING Xiaozhong ZHAO min wang Daning Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China Institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China

Wanbaogou Group-complex is a suit of melange aggregation of the EasternKunlun orogenic belt, which is divided into two parts, i.e. the exotic blocks and the matrix *** on stromatolite fossils yielded in the exotic blocks, the age of this group-complex was oncedefined to the Precambrian. Recently, two Paleogene palynological assemblages have been found insamples from the matrix strata of the Upper Part in this group-complex by the authors: in ascendingorder, the Al-nipollenites-Quercoidites assemblage of Late Eocene and theCaryapollenites-Pinuspollenites assemblage of Early Oligocene. Those results show that the matrixstrata of this melange were mainly formed in Paleogene, and the event causing mixture should be aHimalayan Movement Phase. This paper is designed to provide some detailed evidence for determiningthe age of the matrix strata in this group-complex based upon study at the Kunlunqiao Section, whichwould be of important geological significance for further understanding this group-complex as asuit of melange aggregation and establishing or improving the stratigraphic framework of the studiedregion. The new finding will certainly benefit from now on the investigation on geotectonic andsedimentary evolution of Eastern Kunlun.

关键词： wanbaogou group-complex paleogene palynological assemblages melangeaggregation the eastern Kunlun orogenic belt