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Science China(Life Sciences) 2012年第3期55卷 191-201页

作者： LE Yevgeniya LI Li wang duncheng SCOTT Mark D Canadian Blood Services Vancouver V6T 1Z3 Canada Centre for Blood Research University of British Columbia Vancouver V6T 1Z3 Canada Department of Pathology and Laboratory Medicine University of British Columbia Vancouver V6T 1Z3 Canada

Grafting of methoxypoly(ethylene glycol)(mPEG) to cells and biomaterials is a promising non-pharmacological immunomodulation ***,due to the labile nature of cells,surface-plasma interactions are poorly understood;hence,a latex bead model was *** of beads resulted in a density and molecular weight dependent decrease in total adsorbed protein with a net reduction from(159.9±6.4) ng cm-2 on bare latex to(18.4±0.8) and(52.3±5.3) ng cm-2 on PEGylated beads(1 mmol L-1 of 2 or 20 kD SCmPEG,respectively).SDS-PAGE and iTRAQ-MS analysis revealed differential compositions of the adsorbed protein layer on the PEGylated latex with a significant reduction in the compositional abundance of proteins involved in immune system ***,the biological efficacy of immunocamouflaged cells and materials is mediated by both biophysical obfuscation of antigens and reduced surface-macromolecule interactions.

关键词： PEGylation immunocamouflage iTRAQ mass spectrometry polystyrene latex protein adsorption proteomics meth-oxypoly(ethylene glycol) polymer

Polymer-mediated immunocamouflage of red blood cells: Effects of polymer size on antigenic and immunogenic recognition of allogeneic donor blood cells

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Science China(Life Sciences) 2011年第7期54卷 589-598页

作者： wang duncheng Dana L. KYLUIK Kari L. MURAD Wendy M. TOYOFUKU Mark D. SCOTT Canadian Blood Services Vancouver V6T 1Z3 British Columbia Canada Centre for Blood Research and the Department of Pathology and Laboratory Medicine University of British Columbia Vancouver V6T 1Z3 British Columbia Canada Department of Biology College of St. Rose Albany 12208 New York USA

Developing a practical means of reducing alloimmunization in chronically transfused patients would be of significant clinical benefit. Immunocamouflaging red blood ceils （RBCs） by membrane grafting of methoxypoly（ethylene glycol）（mPEG） may reduce the risk of allo-immunization. The results of this study showed that antibody recognition of non-ABO antigens was sig- nificantly reduced in an mPEG-dose- and polymer size-dependent manner, with higher molecular weight mPEGs providing better immunoprotection. Furthermore, in vivo immunogenicity was significantly reduced in mice serially transfused with mPEG-modified xenogeneic （sheep; sRBCs）, allogeneic （C57B1/6）, or syngeneic （Balb/c） RBCs. Following a primary transfu- sion of sRBCs, mice receiving mPEG-sRBCs showed a 〉90% reduction in anti-sRBC IgG antibody levels. After two transfusions, mice receiving mPEG-sRBCs showed reductions of 〉80% in anti-sRBC IgG levels. Importantly, mPEG-modified autologous cells did not induce neoantigens or an immune （IgG or IgM） response. These data suggest that the global immuno- camouflage of RBCs by polymer grafting may provide a safe and cost-effective means of reducing therisk of alloimmunization.

关键词： methoxypoly(ethylene glycol) erythrocyte blood group antigens mice alloimmunization

A novel erythroid differentiation related gene EDRF2 inhibited α-globin gene expression in K562 cells

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Chinese Science Bulletin 2002年第5期47卷 398-402页

作者： wang duncheng, YANG Xiaoming & SHEN BeifenInstitute of Basic Medical Sciences, Institute of Radiation Medicine Sciences, Academy of Military Sciences, Beijing 100850, China Institute of Basic Medical Sciences Institute of Radiation Medicine Sciences Academy of Military Sciences Beijing China

In previous studies, we found that EDRF2 was an erythroid differentiation related factor, whose expression was markedly upregulated during erythroid differentiation. It suggested that this factor played a role in erythropoiesis. By using rapid amplification of cDNA ends technology, we cloned EDRF2 gene 5’-and 3’-cDNA ends successfully. Transfection and Northern blot analysis demonstrated that EDRF2 inhibited mRNA expression of a-globin gene, while did not regulate γ-globin gene expression. Gel shift assay confirmed that DNA-binding activity of erythroid specific transcription factors GATA-1, NF-E2 and AP1 was not affected by either forced overexpression or artificial down-regulation of EDRF2 gene in K562 cells. However, we detected that the negative regulator of expression of GATA-1 transcription factor was increased in EDRF2 overexpressed K562 cells. These results strongly suggested that EDRF2 was involved in a-globin gene expression and erythroid differentiation and served as a negative regulator of

关键词： globin gene erythroid specific transcription factors leucine zipper RACE EMSA.

A novel erythroid differentiation related gene EDRF1 upregulating globin gene expression in HEL cells

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Chinese Medical Journal 2002年第11期115卷 1701-1705,154-155页

作者：王敦成黎燕沈倍奋军事医学科学院基础医学研究所北京100850

OBJECTIVE: To further characterize the differentiation inducing properties of EDRF1 and demonstrate its functional pathway involved in regulation of globin gene expression. METHODS: By transfecting EDRF1 sense and antisense constructs into HEL cells, we identified the expression of globin and erythropoietin receptor genes by Northern blot analysis. RT-PCR and EMSA (electrophoresis mobility shift assay) were performed to monitor the expression and DNA-binding activity of erythroid specific transcription factors GATA-1 and NF-E2. RESULTS: It was shown that when EDRF1 was overexpressed, production of alpha-globin increased. In antisense EDRF1, overexpression of HEL cells, significant loss of alpha-, gamma-globin mRNA synthesis was observed. The transcription of endogenous GATA-1 and NF-E2 mRNA expression were maintained at the same levels compared with control experiments. However, the transcription activity of GATA-1 was severely impaired. Expression of erythropoietin receptor gene was not influenced by EDRF1 gene overexpression. CONCLUSION: The results suggested that EDRF1 regulated alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor.

关键词： Gene Expression Regulation Cell Differentiation Cells, Cultured DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Erythropoiesis GATA1 Transcription Factor Globins Humans NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit RNA, Messenger Transcription Factors Up-Regulation

Proteomic analysis of immunocamouflaged surfaces

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Chinese Science Bulletin 2012年第25期57卷 3392-3392页

作者： Yevgeniya Le Li Li duncheng wang Mark D. Scott

维普期刊数据库评论

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秦山新建核岛爆破对运行核岛稳态影响研究

资源环境与工程 2009年第F9期23卷 31-36页

作者：奚守仲王敦诚中交公路规划设计院有限公司北京100088 中国核工业集团公司北京100013

秦山二期扩建工程紧邻秦山二期核电站机组,要求实施负挖爆破,因此,研究如何在不影响秦山二期核电机组正常运行的前提下开展扩建工程成为重要的研究课题。通过研究、实验、数值模拟的方法,论证了秦山二期扩建工程进行负挖爆破的可行性,... 详细信息

秦山二期扩建工程紧邻秦山二期核电站机组,要求实施负挖爆破,因此,研究如何在不影响秦山二期核电机组正常运行的前提下开展扩建工程成为重要的研究课题。通过研究、实验、数值模拟的方法,论证了秦山二期扩建工程进行负挖爆破的可行性,确定了最佳爆破方案及单孔最大装药量。

关键词：核电站爆破减震沟

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一种可穿戴式髋关节外骨骼的设计与仿真

机械工程师 2024年第10期 78-83页

作者：莫敦成王建生王宏民五邑大学智能制造学部广东江门529000

为增强下肢外骨骼机器人在医疗方面的康复效果,通过研究人体运动机理及人体髋关节运动数据,设计了一款基于电动机驱动的髋关节外骨骼机器人。首先,对髋关节外骨骼进行结构设计,并建立髋关节外骨骼动力学模型;其次,基于经典PID控制,提出... 详细信息

为增强下肢外骨骼机器人在医疗方面的康复效果,通过研究人体运动机理及人体髋关节运动数据,设计了一款基于电动机驱动的髋关节外骨骼机器人。首先,对髋关节外骨骼进行结构设计,并建立髋关节外骨骼动力学模型;其次,基于经典PID控制,提出了一种基于改进单神经元的PID控制器;最后,在MATLAB/Simulink环境下搭建控制模型并进行仿真实验。仿真结果表明,该控制系统能够使髋关节外骨骼实现良好的跟随效果,提高运动的柔顺性,具有一定的研究意义。

关键词：下肢外骨骼结构设计髋关节改进单神经元的PID控制模拟仿真

基于三级级联架构的接触网定位管开口销缺陷检测

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仪器仪表学报 2019年第10期40卷 74-83页

作者：王昕钰王倩程敦诚吴福庆西南交通大学电气工程学院

针对高铁接触网定位管开口销在列车长期运行振动中容易松脱并且松脱样本数量匮乏的问题,本文提出一种基于深度卷积生成对抗网络(DCGAN),扩充缺陷样本集后,再训练卷积神经网络(CNN)检测开口销缺陷的三级级联架构。该架构首先采用中心点... 详细信息

针对高铁接触网定位管开口销在列车长期运行振动中容易松脱并且松脱样本数量匮乏的问题,本文提出一种基于深度卷积生成对抗网络(DCGAN),扩充缺陷样本集后,再训练卷积神经网络(CNN)检测开口销缺陷的三级级联架构。该架构首先采用中心点法提取训练需要的相同规格开口销图像。然后通过改进的DCGAN生成模拟缺陷样本,并搭建轻量级CNN网络对生成的模拟缺陷样本进行筛选。最后将添加了模拟缺陷样本的扩充缺陷样本集与正样本集输入优化后的VGG16卷积神经网络中,以训练分类模型,检测开口销缺陷。实验结果表明,本文所提方法检测接触网定位管开口销缺陷的准确率高达99%。

关键词：接触网检测深度卷积生成对抗网络开口销缺陷图像生成卷积神经网络

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基于深度学习的接触网顶紧螺栓状态智能检测

铁道学报 2021年第11期43卷 52-60页

作者：程敦诚王倩吴福庆王昕钰牛英杰叶壮西南交通大学电气工程学院四川成都611756

高速铁路接触网支撑装置顶紧螺栓在列车长期运行中会产生松脱和脱落等不良状态,针对其缺陷样本不足、状态变化特征粒度差别小不易判定等问题,提出SSD512和U-net8两种深度学习算法。并基于SSD512定位算法和U-net8语义分割模型的设计,实... 详细信息

高速铁路接触网支撑装置顶紧螺栓在列车长期运行中会产生松脱和脱落等不良状态,针对其缺陷样本不足、状态变化特征粒度差别小不易判定等问题,提出SSD512和U-net8两种深度学习算法。并基于SSD512定位算法和U-net8语义分割模型的设计,实现顶紧螺栓状态的智能检测。通过SSD512实现了顶紧螺栓区域的定位和截取;通过U-net8将顶紧螺栓图片中薄螺母、螺杆等语义信息进行不同颜色的标记;通过语义图片的判定,实现对顶紧螺栓的状态检测。通过在顶紧螺栓的定位、顶紧螺栓的语义分割两个数据集上进行训练、验证,结果表明:所提的顶紧螺栓状态智能检测方法能准确定位接触网中的顶紧螺栓;实现顶紧螺栓的状态检测,综合准确率达到95.75%。

关键词：接触网检测顶紧螺栓状态目标检测语义分割图像识别

矿渣-海水湿排粉煤灰复合胶凝材料的制备研究

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混凝土 2014年第5期 70-72,76页

作者：伏程红倪文王中杰吴辉范敦城北京科技大学金属矿山高效开采与安全教育部重点实验室北京100083

通过优化配合比组分、粒级设计和使用外加剂,制备出一种高掺量矿渣、粉煤灰且使用水泥熟料较少的矿渣-海水湿排粉煤灰基复合胶凝材料。研究了独特的粉磨方式所制备的复合胶凝材料、石膏掺量、矿渣与粉煤灰的掺量及比例对复合高性能胶凝... 详细信息

通过优化配合比组分、粒级设计和使用外加剂,制备出一种高掺量矿渣、粉煤灰且使用水泥熟料较少的矿渣-海水湿排粉煤灰基复合胶凝材料。研究了独特的粉磨方式所制备的复合胶凝材料、石膏掺量、矿渣与粉煤灰的掺量及比例对复合高性能胶凝材料体系强度的影响,初步阐明了复合胶凝材料的活性与级配协同优化效应。复合胶凝材料胶砂水胶比为0.33时具有较好的流动度,胶砂试块养护28d抗压强度可以达到53.53MPa,抗折强度达到13.92MPa,并具有良好的抗硫酸盐侵蚀性能,其氯离子含量符合国家使用标准。

关键词：胶凝材料矿渣海水湿排粉煤灰抗压强度抗侵蚀性能