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Protective effect of retaining wall on rock avalanche:A case study of Nayong rock avalanche in China

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Journal of Mountain Science 2024年第4期21卷 1215-1230页

作者： wang zhongfu SHI Fengge HE Siming ZHANG Xusheng wang Jingying LIU Enlong North China University of Water Resources and Electric Power Zhengzhou 450046China Hunan Provincial key Laboratory of key Technology on Hydropower Development Changsha 410014China Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China College of Water Resource and Hydropower Sichuan UniversityChengdu 610065China

Rock avalanches are generally difficult to prevent and control due to their high velocities and the extensive destruction they ***,barrier structures constructed along the path of a rock avalanche can partially mitigate the magnitudes and consequences of such catastrophic *** selected a rock avalanche in Nayong County,Guizhou Province,China as a case to study the effect of the location and height of a retaining wall on the dynamic characteristics of rock avalanche by using both actual terrain-based laboratory-model tests and coupled PFC3D-FLAC3D numerical *** findings demonstrate that a retaining wall can largely block a rock avalanche and its protective efficacy is significantly influenced by the integrity of the retaining *** numerical simulation can serve as a powerful tool for analyzing the interaction between a rock avalanche and a retaining wall,facilitating precise observations of its deformation and *** impact-curve characteristics of the retaining wall depend upon whether or not the rock avalanche-induced destruction is taken into *** location of the retaining wall exerts a greater influence on the outcome compared to the height and materials of the retaining wall,while implementing a stepped retaining-wall pattern in accordance with the terrain demonstrates optimal efficacy in controlling rock avalanche.

关键词： Rock avalanche Laboratory model test Retaining wall PFC^(3D) FLAC^(3D) Impact force

An innovative method used for the identification of N-glycans on soybean allergenβ-conglycinins

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Food Science and Human Wellness 2023年第3期12卷 842-850页

作者： Cheng Li Yang Tian Jianli Han Yu Lu Meiyi Zou Yue Jia Chengjian wang Linjuan Huang zhongfu wang Key Laboratory of Glycobiology and Glycoengineering of Xi’an College of Food Science and TechnologyNorthwest UniversityXi’an 710069China College of Life Sciences Northwest UniversityXi’an 710069China

β-Conglycinin is one of the major allergens existed in soybean.N-Glycans attached to theβ-conglycinin influenced the immunoreactivity and antigen presenting efficiency ofβ-*** this study,we described a new method used to release and collect the N-glycans fromβ-conglycinin,and the N-glycans existed in linear epitopes ofβ-conglycinin were *** hydrolyzed fromβ-conglycinin were purified by cotton hydrophilic *** acid was then used to release glycans from glycopeptides,and new glycopeptides containing one single N-acety1-D-glucosamine(G1 cNAc)moiety were then utilized for mass *** glycosylation sites(Asn-199,Asn-455,Asn-215,Asn-489 and Asn-326)and 22 kinds of glycopeptides were *** is noteworthy that the peptide VVN^(#)ATSNL(where^(#)represents for the glycosylation site)was analyzed to be both glycopeptide and linear *** results provided a new method for the N-glycoform analysis of food allergens,and laid a foundation for understanding the relationship between glyco sylation and food allergy.

关键词： Soybean allergenβ-conglycinin Glycopeptide Mass spectrometry N-Glycan Glycosylation site

Capture-Release Strategy Facilitates Rapid Enzymatic Assembly of Oligosaccharides

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Chinese Journal of Chemistry 2022年第3期40卷 343-350页

作者： Wenyuan Fang Kan Zhong Jiansong Cheng Xian-Wei Liu Chang-Cheng Liu zhongfu wang Hongzhi Cao National Glycoengineering Research Center NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicineand Shandong Key Laboratory of Carbohydrate Chemistry and GlycobiologyShandong UniversityQingdaoShandong 266237China Key Laboratory of Marine Drugs of Ministry of Education Shandong Key Laboratory of Glycoscience and GlycotechnoiogySchool of Medicine and PharmacyOcean University of ChinaQingdaoShandong 266003China College of Pharmacy Nonkoi UniversityTianjin 300071China Glycobiology and Glycotechnology Research Center College of Food Science and TechnologyNorthwest UniversityXi'anShaanxi 710069China Laboratory for Marine Drugs and Bioproducts Pilot Notional Laboratory for Marine Science and Technology(Qingdao)QingdaoShandong 266237China

A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of *** acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide ***,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and *** strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme modules for the rapid assembly of a series of complex oligosaccharides.

关键词： Glycosylation Oligosaccharide synthesis Thiol tag Capture-release Biocatalysis

考虑地震加速度时程影响的挡土墙动土压力分布

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四川大学学报（工程科学版） 2015年第4期47卷 31-37页

作者：何思明王忠福王娟中国科学院山地灾害与地表过程重点实验室四川成都610041 中国科学院水利部成都山地灾害与环境研究所四川成都610041 中国科学院青藏高原地球科学卓越创新中心北京100101 中国科学院大学北京100049

针对传统拟静力法在计算挡土墙动土压力时不能考虑地震荷载等特点的问题,采用拟动力法,考虑地震加速度随时间及挡土墙深度的变化,采用薄层分析法并结合极限平衡理论,推导了地震荷载作用下挡土墙动土压力随地震加速度时程变化的表达式,... 详细信息

针对传统拟静力法在计算挡土墙动土压力时不能考虑地震荷载等特点的问题,采用拟动力法,考虑地震加速度随时间及挡土墙深度的变化,采用薄层分析法并结合极限平衡理论,推导了地震荷载作用下挡土墙动土压力随地震加速度时程变化的表达式,研究了墙后破裂面倾角、动土压力系数、动土压力合力作用点位置随地震时程的变化特征及地震加速度对挡土墙动土压力分布的影响。结果表明:动土压力合力作用点位置随地震加速度时程呈周期性变化,合力作用点高度高于传统方法所确定的H/3;地震荷载下挡土墙主动土压力呈非线性分布,地震加速度、内摩擦角对动土压力分布有显著影响。

关键词：地震挡土墙拟动力学方法动土压力分布合力作用点

Highly Sensitive MALDI-TOF-MS Analysis of Reducing Glycan by On-target Derivatization with Hydrazines

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Highly Sensitive MALDI-TOF-MS Analysis of Reducing Glycan by...

An Expedious Synthesis of beta-Glycosyl Fluoride

中国生物工程学会第十一届学术年会暨2017年全国生物技术大会

作者： Ying Zhang Bo wang Wanjun Jin Chengjian wang Linjuan Huang zhongfu wang Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education and Provincial Key Laboratory of Biotechnology College of Life Sciences Northwest University

Glycan have been found to participate in many important biological *** analysis of glycans by matrixassisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) is of great importance but significantly hampered by their low ionization efficiency or sialic acid *** derivatization offer an viable way to improve both the ionization efficiency and analytical sensitivity of the sugar sample in MS analysis by altering theirhydrophobicity or charge ability.

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An Expedious Synthesis of beta-Glycosyl Fluoride

Chemical synthesis of UDP-2-acetonyl-Gal from its corresponding natural saccharide

全国第二届海洋与陆地多糖多肽及天然创新药物研发学术会议

作者： Xiaohu wang Jie Fu Xiangguang Tian Jianbo Zhang zhongfu wang Department of Chemistry East China Normal University School of Life Sciences Northwestern University

The reaction of a series of peracetylated pyranoses with 5-H-3-oxa-l,1,2,2,4,4,5,5-octafluoropentanesulfonyl fluoride(HCF2CF2OCF2CF2SO2F)in the presence of DBU was investigated resulting in the smooth fonnation of corresponding glycosyl fluorides in excellent yields of 64%7%,while its anomeric configuration is beta *** the glycosyl fluorides are well characterized with NMR

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Chemical synthesis of UDP-2-acetonyl-Gal from its correspond...

第二届糖合成化学青年学术研讨会

作者：张英 Mingming Yang Hongdan Shi Linjuan Huang zhongfu wang 西北大学 Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University

O-GlcNAcylations,one of the most universal and important post-translational modifications of protein,take charge of plenty of cellular-biological processes and are associated with variety of diseases such as diabetes and tumor and Alzheimer’s disease when their glycosylations are at abnormal expression ***,the detection of intracellular protein O-Glc NAc glycosylated modifications,can be used as a research reference for the study of occurrence and development of disease and pathogenesis[1].Due to the structure complexity and micro-scale expression within the cell,the detecting and analyzing O-Glc NAc glycosylation have suffered from some drawbacks including lack of selective assay for detection or capturing the low-abundance O-Glc NAcylated ***,the comprehensive studies on function-structure mechanism of protein O-Glc NAcylation that involve in disease development progress have been seriously hindered these years.A chemical-enzymic reaction that could enable to add active acetonyl-containing Galactose（UDP-2-keto-Gal）to C-4 position of O-Glc NAc moiety on OGlc NAcylation protein,provide an effective avenue for selectively and efficiently detecting the target glycoprotein by using hydrazide containing chemicals[2].However,the ketone-substrate analogues are not commercialized,leading to that the detection of the O-Glc NAcylated modification is severely *** present study focused on the establishment of a universal chemical method for the synthesis of ketone substrate *** our work,we mainly made these innovations as follows:（1）The synthesis of glycal from Gal was conducted by an one-pot method without the need of purification,which could reduce the loss of sample and simplify the reaction steps;（2）Osmium tetroxide and sodium periodate method is used to replace ozone oxidation of carbon-carbon double bond,to generate keto sugar under room temperature;（3）In the synthesis of UDP-ketone-sugar,the C-1 was converted to be the trichloroac

关键词： O-GlcNAc UDP-2-acetonyl-Gal chemical synthesis

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糖蛋白的凝胶电泳和电印迹染色鉴定技术

化学进展 2008年第7期20卷 1158-1164页

作者：张英黄琳娟王仲孚西北大学生命科学学院西部资源生物与现代生物技术省部共建教育部重点实验室陕西省生物技术重点实验室西安710069

糖基化是蛋白质翻译后修饰最重要的方式之一,是必需的生理过程,对蛋白质生物学功能的正确发挥起着至关重要的作用。二维凝胶电泳(two-dimensional gel electrophoresis,2-DE)是常用的分离蛋白质的方法之一,糖蛋白在凝胶上的检测是分离... 详细信息

糖基化是蛋白质翻译后修饰最重要的方式之一,是必需的生理过程,对蛋白质生物学功能的正确发挥起着至关重要的作用。二维凝胶电泳(two-dimensional gel electrophoresis,2-DE)是常用的分离蛋白质的方法之一,糖蛋白在凝胶上的检测是分离鉴定糖蛋白的关键之一。本文对近年来凝胶电泳、电印迹技术中鉴定糖蛋白的衍生试剂的研究和应用进行了综述,对多种衍生试剂衍生糖蛋白的机理作了介绍。

关键词：糖蛋白凝胶电泳电印迹染色

城市居民对旅游经济和环境影响因素感知的结构方程分析——以大连市为例

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四川师范大学学报（社会科学版） 2011年第1期38卷 70-78页

作者：王忠福张利西安邮电学院经济与管理学院西安710061

本文在国内城市居民旅游影响感知量表开发的基础上,首先对初试调查问卷进行信度和效度检验,形成正式问卷,接着对正式问卷进行大样本调查,之后运用结构方程模型来综合考虑居民人口统计学特征、居民旅游相关度、居民旅游经济影响感知、居... 详细信息

本文在国内城市居民旅游影响感知量表开发的基础上,首先对初试调查问卷进行信度和效度检验,形成正式问卷,接着对正式问卷进行大样本调查,之后运用结构方程模型来综合考虑居民人口统计学特征、居民旅游相关度、居民旅游经济影响感知、居民旅游环境影响感知、居民旅游社会文化影响感知、居民旅游发展态度和满意总体感知以及居民参与旅游发展经营管理水平感知,实证检验居民旅游影响之间的相互关系。检验表明,居民人口学特征对旅游正面影响具有正向的感知作用,对旅游负面影响具有负向的感知作用;居民旅游相关度对旅游正面影响具有负向的感知作用,对旅游负面影响具有正向的感知作用;旅游影响对旅游感知具有正向的促进作用。

关键词：居民旅游影响感知问卷调查结构方程模型实证检验