检索结果-南通市图书馆

Establishment of Hamster-and Human-PRNP Transgenic Mice

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2011年第6期24卷 608-616页

作者： GONG Han Shi TIAN Chan ZHANG Bao yun wang zhao yun XIE Wu Ling JING Yuan Yuan GAO Chen JIANG Hui Ying SHI Qi LIU Yong DONG Xiao Ping School of Medicine Xi＇an Jiao Tong University Xi＇an 710061 Shaanxi China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To create transgenic mice expressing hamster- and human-PRNP as a model tor understanding the physiological function and pathology of prion protein （PrP）, as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies （TSEs）. Methods Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin （H ＆ E） staining and immunohistochemical （IHC） methods. Results Integrated PRNP copy number in various mouse lines was 53 （Tg-haPrP1）, 18 （Tg-huPrP1）, 3 （Tg-huPrP2）, and 16 （Tg-huPrP5）, respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs. Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.

关键词： PrP PRNP Transgenic mice Copy number

Safety Observation Study on Haemophilus Influenza Type B Conjugate Vaccines Injected at Different Sites in Chinese Infants

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第8期26卷 693-696页

作者： LUO Feng Ji SHI Nian Min LI Li ZHANG Zheng AI Xing YANG Li Qing BAI yun Hua wang zhao yun LU Qiang Chaoyang District Center for Disease Control and Prevention

In the present study, the safety of Hoemophilus influenza type b conjugate vaccines inoculated in the upper arm deltoid and vastus lateralis muscle was evaluated. 680 infants aged 2-5 months and 6-12 months were selected to be the research subjects in whom the Hib conjugate vaccines were inoculated by injection in the upper arm deltoid and vastus lateralis muscle, respectively. The safety analysis indicated that there were no statistic differences in the incidence rates of adverse reactions when the Hib conjugate vaccines were inoculated at different sites. So we concluded that the safety of inoculation injection of Hib conjugate vaccines in vastus lateralis muscle was the same as that inoculated in the upper arm deltoid.

关键词： In Safety Observation Study on Haemophilus Influenza Type B Conjugate Vaccines Injected at Different Sites in Chinese Infants

扩展现实(XR)技术在法学教学中的价值与应用

在线全文

学校读者我要写书评

暂无评论

教育理论与实践 2021年第30期41卷 62-64页

作者：王运召河南省教育科学研究院河南郑州300387

扩展现实(XR)技术具有交互性、智能化、情境性、沉浸性等特质,可以为学习者带来“沉浸、交互、构想”体验。扩展现实技术应用于法学教学中,可以实现虚拟场景可视化,使营造的虚拟环境具有真实性和想象性。在法学教学中引入扩展现实技术,... 详细信息

扩展现实(XR)技术具有交互性、智能化、情境性、沉浸性等特质,可以为学习者带来“沉浸、交互、构想”体验。扩展现实技术应用于法学教学中,可以实现虚拟场景可视化,使营造的虚拟环境具有真实性和想象性。在法学教学中引入扩展现实技术,能实现感观模拟的功能创新,赋予法学教学类人化感知;进行客观事实的情境再造,助力法学教学的多维度整合;完成沉浸场景的智能重构,实现法学教学中的情境叠加。

关键词：扩展现实技术法学教学虚拟场景可视化真实性想象性类人化感知多维度整合情境叠加

Liquidus and phase equilibria in CaO-SiO2-5%MgO-20%Al2O3-TiO2 system

在线全文

学校读者我要写书评

暂无评论

Journal of Central South University 2017年第1期24卷 48-55页

作者：孙丽枫石俊杰张波邱吉雨王昭云姜茂发 School of Metallurgy Northeastern UniversityShenyang 110819China Key Laboratory for Ecological Metallurgy of Multimetallic Ores of Ministry of Education(Northeastern University) Shenyang 110819China

The single hot thermocouple technique （SHTT） and high temperature equilibrium technique were combined to investigate the phase diagram of the CaO-SiO2-5%MgO-20%AlzO3-TiO2 system. The 1300 ℃ to 1500 ℃ liquidus lines are calculated according to the thermodynamic equations based on the pseudo-melting temperatures measured by the single hot thermocouple technique. The phase equilibria relationships are experimentally determined at 1400 ℃ using the high temperature equilibria technique followed by X-ray fluorescence （XRF）, X-ray diffraction（XRD）, scanning electron microscopy （SEM） and energy dispersive X-ray spectroscopy （EDX） analysis. The liquid phase（L）, melilite solid solution phase （（C2MSz,C2AS）ss）, diopside phase（CMS2） and perovskite phase （CaO·TiO2） are found. Coupled with the liquidus lines and equilibria results, the phase diagram is constructed for the specified region of the CaO-SiO2-5%MgO-20%Al2O3-TiO2 system.

关键词： phase diagram liquidus equilibrium TiO2 single hot thermocouple technique (SHTT) thermodynamics

在线全文

学校读者我要写书评

暂无评论

中国中、低丰度大油气田基本特征及形成条件

石油勘探与开发 2008年第6期35卷 641-650页

作者：赵文智汪泽成王红军王兆云中国石油天然气股份有限公司中国石油勘探开发研究院

近年中国陆上发现的一批中、低丰度大型油气田具有低孔渗、小油(气)柱、低储量丰度和规模大等特点。中、低丰度油气藏大面积成藏的有利条件是:大型敞流湖盆腹地发育大型牵引流成因砂体,与烃源岩呈"三明治"结构大面积间互,为成藏奠定了基... 详细信息

近年中国陆上发现的一批中、低丰度大型油气田具有低孔渗、小油(气)柱、低储量丰度和规模大等特点。中、低丰度油气藏大面积成藏的有利条件是:大型敞流湖盆腹地发育大型牵引流成因砂体,与烃源岩呈"三明治"结构大面积间互,为成藏奠定了基础;小油气柱、常压—低压系统降低成藏对盖层质量的要求;储集体内部非均质性强烈,气藏整体连通性差,降低了气体逸散能量,保证油气在包括地质条件相对劣质区的大范围成藏;抬升卸载环境导致气源岩解吸面状排烃,有利于晚期大面积成藏。研究表明,中国陆上大型坳陷盆地向斜区具有发现大型岩性油气藏的良好前景,这一认识已在鄂尔多斯、松辽和四川盆地向斜区油气勘探中发挥了指导作用,推动油气储量大幅度增长,未来仍有良好发展潜力。

关键词：大油气田大面积成藏中低丰度岩性油气藏理论新进展坳陷盆地

Phylogenetic Analysis of Homologous Proteins Encoded by UL2 and UL23 genes of Herpesviridae

在线全文

学校读者我要写书评

暂无评论

中国病毒学 2007年第3期22卷 207-211页

作者： Long-ding LIU　 Wen-juan WU　 Min HONG　 Hai-jing SHI　 Shao-hui MA　 Jing-jing wang　 Hong-ling zhao　 yun LIAO　 Qi-han LI　 Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College Kunming 650118 China

The proteins encoded by the Herpesviridae β-gene play a critical role in the replication stage of the *** this paper,phylogenetic analyses provided evidence that some β-gene products,such as UL2 and UL23 from HSV1,have their homologous genes in its family,and also exist in prokaryotic organisms,indicating that these viruses appear to have been assembled over evolutionary time by numerous independent events of horizontal gene transfer.

关键词：系统分析同源蛋白质编码疱疹病毒 UL2基因 β蛋白质 UL23基因

在线全文

学校读者我要写书评

暂无评论

高低语境下对话式语音交互系统研究

Unraveling the significance of cobalt on transformation kinetics,crystallography and impact toughness in high-strength steels

包装工程 2021年第10期42卷 85-91页

作者：覃京燕王赟曌北京科技大学北京 100083

目的对话式语音交互在智能产品中的地位越来越重要,是人机交互系统中的重要方式.但由于用户和产品间存在诸多差异,造成对话式语音交互在用户体验方面表现得不尽如人意.高低语境是用来研究跨文化交际的一种经典理论,用于保证处在不同语... 详细信息

目的对话式语音交互在智能产品中的地位越来越重要,是人机交互系统中的重要方式.但由于用户和产品间存在诸多差异,造成对话式语音交互在用户体验方面表现得不尽如人意.高低语境是用来研究跨文化交际的一种经典理论,用于保证处在不同语境下的群体可以有效沟通.通过侧重研究对话式语音交互中的语境差异,从高低语境的视角出发,寻找相关案例,提供建议.方法通过文献研究和实例研究方法,梳理对话式语音交互的发展流程,语境问题的形成原因以及具体实例的现状和优化策略.结论通过分析现阶段的具体案例,将语言学中的理论和具体方法引入设计研究中,为对话式语音交互的具体实践提供新的改进思路.

关键词：跨文化交际语音交互交互语境设计方法

来源：评论

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 380-390页

作者： Yishuang Yu Jingxiao zhao Xuelin wang Hui Guo Zhenjia Xie Chengjia Shang Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China

This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic *** results show that the Co-bearing steel exhibits finer blocks and a lower ductile-brittle transition temperature than the steel without ***,the Co-bearing steel reveals higher transformation rates at the intermediate stage with bainite volume fraction ranging from around 0.1 to *** improved impact toughness of the Co-bearing steel results from the higher dense block boundaries dominated by the V1/V2 variant ***,the addition of Co induces a larger transformation driving force and a lower bainite start temperature(BS),thereby contributing to the refinement of blocks and the increase of the V1/V2 variant *** findings would be instructive for the composition,microstructure design,and property optimization of high-strength steels.

关键词： high-strength steel cobalt transformation kinetics crystallography impact toughness

有机质“接力成气”模式的提出及其在勘探中的意义

在线全文

学校读者我要写书评

暂无评论

石油勘探与开发 2005年第2期32卷 1-7页

作者：赵文智王兆云张水昌王红军赵长毅胡国义中国石油勘探开发研究院中国石油勘探开发研究院廊坊分院

有机质“接力成气”机理是指成气过程中生气母质的转换和生气时机与贡献的接替。有两层含义:一是干酪根热降解成气在先,液态烃和煤岩中可溶有机质热裂解成气在后,二者在成气时机和先后贡献方面构成接力过程;二是干酪根热降解形成的液态... 详细信息

有机质“接力成气”机理是指成气过程中生气母质的转换和生气时机与贡献的接替。有两层含义:一是干酪根热降解成气在先,液态烃和煤岩中可溶有机质热裂解成气在后,二者在成气时机和先后贡献方面构成接力过程;二是干酪根热降解形成的液态烃只有一部分可排出烃源岩,形成油藏,相当多的部分则呈分散状仍滞留在烃源岩内,在高—过成熟阶段会发生热裂解,使烃源岩仍具有良好的生气潜力。这一理论的提出,回答了我国热演化高-过成熟地区勘探潜力问题与天然气晚期成藏的机理问题,对拓搌勘探领域有重要意义。

关键词：接力成气机理干酪根热降解气液态烃热裂解气煤岩可溶有机质生气勘探领域