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Energy & Environmental Materials 2024年第5期7卷 391-403页

作者： Zhimin Dong Donglin Gao Zifan Li Haonan Pei Lin Xu Jian Huang Xiaohong Cao youqun wang Ting wang Qianglin Wei Zhibin Zhang Yunhai Liu State Key Laboratory of Nuclear Resources and Environment East China University of Technology Institute of Geology Chinese Academy of Geological Sciences

Piezo-photocatalysis could coalesce the advantages of mechanical vibration and solar energy perfectly to achieve high-efficiency catalytic activity. Herein,the quintessential piezoelectric material CdS nanowires with different aspect ratios are precisely constructed and applied for piezo-photocatalytic reduction of U（Ⅵ） for the first time. The ultrasonic （60 kHz, 100 W） induces piezoelectric potential to generate a 0.57 eV A-1electric field, which is added to the direction of CdS （010） as a driving force to efficiently separate photogenerated charges. The alliance between piezoelectric effect and photocatalytic activity endows CdS NW-3 with the fastest piezophotocatalytic rate under ultrasonic vibration and 5 W LED irradiation, and the relevant rate constant (0.042 min-1) is about 12 and 53.8 times than that of LED and ultrasonication. More importantly, 93.74%of U（Ⅵ） could be removed from natural uranium mine wastewater. Therefore, this piezophotocatalysis system that reduces U（Ⅵ） to easily separable （UO2）O2·2H2O（s） provides valuable input for disposal applications of radioactive wastewater and broadens the horizons of nuclear energy utilization toward the advancement of carbon neutrality.

关键词： (UO2)O2·2H2O CdS nanowires piezo-photocatalytic piezo-potential U(VI)

Plant grafting and its application in biological research

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Chinese Science Bulletin 2011年第33期56卷 3511-3517页

作者： wang youqun State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 China

Grafting is widely used for various aspects of plant biological research. We reviewed grafting methods and graft development processes for herbaceous plants. We introduced also methods of investigating the development of graft union and compatibility, and the application of grafting in understanding translocation of substances and long-distance signaling in plants.

关键词：植物生物学嫁接方法应用程序草本植物兼容性移植

Post-transcriptional gene silencing signal could move rapidly and bidirectionally in grafted Arabidopsis thaliana

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Chinese Science Bulletin 2006年第3期51卷 313-319页

作者： LI Ming JIANG Shiling wang youqun LIU Guoqin State Key Laboratory of Plant Physiology and Biochemistry College ofBiological Sciences China Agricultural University Beijing 100094China

RNA interference (RNAi), one of the newly found ways for post-transcriptional gene si- lencing, has been widely used to investigate gene functions through transgenic methods for introducing an RNA silencing signal into plants. In the present study, we constructed a dexamethazone (DEX)-inducible RNAi binary vector harboring a specific sequence fragment (168-bp) homologous to KatB and KatC, two kinesin isoform genes of Arabidopsis, which were proved to result in the post-transcriptional gene si- lencing of KatB and KatC in DEX-induced transgenic plants. RT-PCR and Northern blot analysis on trans- genic homozygous Arabidopsis (termed as RNAi-type plants) showed that DEX inducement causes KatB and KatC mRNA degradation. With a simplified method, Arabidopsis grafting was effectively per- formed between RNAi-type and wild-type lines. The target gene mRNA levels were tested based on semi-quantitative RT-PCR. Our results demonstrateed that DEX-induced gene silencing signals could result in a reduction in KatB and KatC mRNA in the wild-type rootstocks or scions, indicating that silencing signals of RNAi could be transmitted bidirectionally across the graft junction whether RNAi-plants were scions or stocks. In contrast to the previously reported results on grafted tobacco, the transmission of post- tran- scriptional gene silencing signals caused by RNAi in grafted Arabidopsis is more effective than that in to- bacco.

关键词：阿布属嫁接 RNA干预基因抑制信号传导烟草

Localization of NOS-like protein in guard cells of Vicia faba *** its possible function

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Chinese Science Bulletin 2007年第1期52卷 84-90页

作者： LIU Xin wang youqun JIA WenSuo LOU ChengHou ZHANG ShuQiu State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beiiing 100094 China Department of Life Science Lai Yang Agricultural College Qingdao 266109 China

Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of ni- tric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS in- hibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO genera- tion from NOS pathway is the main pathway in the stress and JA responses. The pharmacological ex- periment showed that increasing the Ca2+ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca2+-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses.

关键词：免疫细胞氧化一氮连位化合物生物化学

Rab-H_1b is essential for trafficking of cellulose synthase and for hypocotyl growth in Arabidopsis thaliana

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Journal of Integrative Plant Biology 2018年第11期60卷 1051-1069页

作者： Ming He Miao Lan Baocai Zhang Yihua Zhou youqun wang Lei Zhu Ming Yuan Ying Fu State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University State Key Laboratory of Plant Genomics and National Plant Gene Research Center Institute of Genetics and Developmental Biologythe Chinese Academy of Sciences

Ceil-wall deposition of cellulose microfibrils is essential for plant growth and development. In plant cells, cellulose synthesis is accomplished by cellulose synthase complexes located in the plasma membrane. Trafficking of the complex between endomembrane compartments and the plasma membrane is vital for cellulose biosynthesis; however, the mechanism for this process is not well understood. We here report that, in Arabidopsis thaliana, Rab-H1b, a Golgi-localized small GTPase, participates in the trafficking of CELLULOSE SYNTHASE 6 （CESA6） to the plasma membrane. Loss of Rab-Hlb function resulted in altered distribution and motility of CESA6 in the plasma membrane and reduced cellulose content. Seedlings with this defect exhibited short, fragile etiolated *** of CESA6 was impaired in rab-Mb cells, and endocytosis in mutant cells was significantly reduced as well. We further observed accumulation of vesicles around an abnormal Golgi apparatus having an increased number of cisternae in rab-Mb cells, suggesting a defect in cisternal homeostasis caused by Rab-Hlb loss function. Our findings link Rab GTPases to cellulose biosynthesis, during hypo- cotyl growth, and suggest Rab-Hlb is crucial for modulating the trafficking of cellulose synthase complexes between endomembrane compartments and the plasma membrane and for maintaining Golgi organization and morphology.

关键词： Rab-H1b is essential for trafficking of cellulose synthase and for hypocotyl growth in Arabidopsis thaliana

U(VI) adsorption behavior onto polypyrrole coated 3R-MoS2 nanosheets prepared with the molten salt electrolysis method

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International Journal of Minerals,Metallurgy and Materials 2022年第3期29卷 479-489页

作者： Yuhui Liu Meng Tang Shuang Zhang Yuling Lin Yingcai wang youqun wang Ying Dai Xiaohong Cao Zhibin Zhang Yunhai Liu State Key Laboratory of Nuclear Resources and Environment East China University of TechnologyNanchang 330013China School of Nuclear Science and Engineering East China University of TechnologyNanchang 330013China

To improve the separation capacity of uranium in aqueous solutions, 3R-MoS2 nanosheets were prepared with molten salt electro- lysis and further modified with polypyrrole (PPy) to synthesize a hybrid nanoadsorbent (PPy/3R-MoS2). The preparation conditions of PPy/3R- MoS2 were investigated and the obtained nanosheets were characterized with scanning electron microscope (SEM), high resolution transmis- sion electron microscope (HRTEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectro- scopy (XPS). The results showed that PPy/3R-MoS2 exhibited enhanced adsorption capacity toward U(VI) compared to pure 3R-MoS2 and PPy;the maximum adsorption was 200.4 mg/g. The adsorption mechanism was elucidated with XPS and FTIR: (1) negatively charged PPy/3R-MoS2 nanosheets attracted by an electrostatic interaction;(2) exposed C, N, Mo, and S atoms complexed with U(VI) through co- ordination;(3) Mo in the complex partly reduced the adsorbed U(VI) to U(IV), which further regenerated the adsorption point and continu- ously adsorbed U(VI). The design of the PPy/3R-MoS2 composite with a high adsorption capacity and chemical stability provides a new direc- tion for the removal of radionuclide.

关键词： molten salt electrolysis 3R-MoS2 nanosheets polypyrrole modification adsorption uranium

Synergy of photocatalytic reduction and adsorption for boosting uranium removal with PMo_(12)/UiO-66 heterojunction

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Chinese Chemical Letters 2022年第7期33卷 3577-3580页

作者： Zhibin Zhang Zifan Li Zhimin Dong Fengtao Yu Yingcai wang youqun wang Xiaohong Cao Yuhui Liu Yunhai Liu Jiangxi Province Key Laboratory of Synthetic Chemistry East China University of TechnologyNanchang 330013China State Key Laboratory of Nuclear Resources and Environment East China University of TechnologyNanchang 330013China

In this work,we proposed a new U(Ⅵ)removal strategy combining adsorption and photocatalytic reduction by the PMo_(12)/UiO-66 *** PMo_(12)has been encapsulated in the cavities of Ui O-66 by a one-step hydrothermal method,and the PMo_(12)/UiO-66 exhibited high adsorption capacity and photocatalytic *** maximal theoretical sorption capacity of U(Ⅵ)on 15%PMo_(12)/UiO-66 reached225.36 mg/g and the photoreduction rate of 15%PMo_(12)/UiO-66 is about thirty times as much as *** the light irradiation,the photogenerated electrons rapidly transport from UiO-66 to PMo_(12),and the photo-generated electrons could efficiently reduce the pre-enriched U(Ⅵ)to U(IV).This work provides new insights into remediation of the radioactive environment.

关键词： UiO-66 PMo12 Uranium Adsorption Photocatalytic reduction

磁性介孔碳负载羟基磷灰石的制备及对U(Ⅵ)的吸附性能

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湿法冶金 2024年第3期43卷 265-274,283页

作者：曾冬玲陈磊郑智阳张志宾曹小红王有群刘云海东华理工大学核资源与环境国家重点实验室江西南昌330000 核工业二九〇研究所广东韶关512029

采用共沉淀法研究制备了不同质量比的磁性介孔碳(MMC)与羟基磷灰石(HAP)复合材料(MMC@HAP-x,x=1、4和6)并用于去除溶液中U(Ⅵ)。采用XRD、FT-IR、Zeta等方法表征了MMC、HAP和MMC@HAP-x的结构、功能基团及表面电位等理化性质。探讨了MMC@... 详细信息

采用共沉淀法研究制备了不同质量比的磁性介孔碳(MMC)与羟基磷灰石(HAP)复合材料(MMC@HAP-x,x=1、4和6)并用于去除溶液中U(Ⅵ)。采用XRD、FT-IR、Zeta等方法表征了MMC、HAP和MMC@HAP-x的结构、功能基团及表面电位等理化性质。探讨了MMC@HAP-x吸附U(Ⅵ)的动力学、热力学及吸附机制。结果表明:MMC@HAP-x对U(Ⅵ)的吸附最佳pH均为4.0,达到吸附平衡所需时间均小于10 min,MMC@HAP-6的理论饱和吸附量可达1164.62 mg/g,且吸附过程是自发的化学吸附过程。U(Ⅵ)在MMC@HAP-x的固定是由于HAP与UO_(2)^(+2)相互作用形成了Ca(UO_(2))_(2)(PO_(4))_(2)·3H_(2)O。MMC@HAP-6是一种快速的铀吸附剂,有望用于放射性废水中铀的去除。

关键词：磁性介孔碳羟基磷灰石 U(Ⅵ) 吸附

维普期刊数据库博看期刊

Numerical Prediction of Ride Comfort of Tracked Vehicle Equipped with Novel Flexible Road Wheels

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Chinese Journal of Mechanical Engineering 2024年第4期37卷 277-289页

作者： Yaoji Deng Zhiyue wang youqun Zhao Junjie Gong Hui Shen Fen Lin College of Mechanical Engineering Yangzhou UniversityYangzhou 225127China College of Energy and Power Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China

Enhancing ride comfort has always constituted a crucial focus in the design and research of modern tracked vehicles,heavily reliant on the driving system's *** the road wheel is a key component of the driving system,traditional road wheels predominantly adopt a solid structure,exhibiting subpar adhesion performance and damping effects,thereby falling short of meeting the demands for high-speed,stable,and long-distance driving in tracked *** this issue,this paper proposes a novel type of flexible road wheel(FRW)characterized by a catenary *** study investigates the ride comfort of tracked vehicles equipped with flexible road wheels by integrating finite element and vehicle ***,three-dimensional(3D)finite element(FE)models of both flexible and rigid road wheels are established,considering material and contact *** models are validated through a wheel radial loading *** on the validated FE model,the paper uncovers the relationship between load and radial deformation of the road wheel,forming the basis for a nonlinear mathematical ***,a half-car model of a tracked vehicle with seven degrees of freedom is established using Newton's second law.A random road model,considering the track effect and employing white noise,is *** study concludes by examining the ride comfort of tracked vehicles equipped with flexible and rigid road wheels under various speeds and road *** results demonstrate that,in comparison to the rigid road wheel(RRW),the flexible road wheel enhances the ride comfort of tracked vehicles on randomly uneven *** research provides a theoretical foundation for the implementation of flexible road wheels in tracked vehicles.

关键词： Ride comfort Flexible road wheel Finite element model Dynamic model Tracked vehicle

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徒手切片与植物细胞微管免疫荧光定位

农业生物技术学报 1999年第4期7卷 382-385页

作者：李岩王幼群中国农业大学生物学院!北京 100094

报道一种植物细胞微管骨架免疫荧光定位的新方法。化学固定后，通过徒手切片，将南瓜茎沿纵切面切成100～200μm的薄片，经过免疫荧光定位方法处理，在共焦激光扫描显微镜下，可以对不同类型细胞中微管骨架的排列进行观察。研究发现，... 详细信息

报道一种植物细胞微管骨架免疫荧光定位的新方法。化学固定后，通过徒手切片，将南瓜茎沿纵切面切成100～200μm的薄片，经过免疫荧光定位方法处理，在共焦激光扫描显微镜下，可以对不同类型细胞中微管骨架的排列进行观察。研究发现，南瓜茎细胞内微管骨架排列的主要方式有以下几种：1、网络状微管，多存在于薄壁细胞中，其中微管往往与液泡膜、核膜及质膜相连；2、周质微管，多存在于导管分子或伸长细胞中，其中微管往往彼此平行，并与细胞的纵轴成一定角度；3、纵向微管，存在于筛分子等少数伸长细胞中，其中微管往往接近与细胞纵轴平行。

关键词：微管免疫荧光定位徒手切片植物细胞南瓜