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Dietary aflatoxin B1 induces abnormal deposition of melanin in the corium layer of the chicken shank possibly via promoting the expression of melanin synthesis-related genes

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Journal of Integrative Agriculture 2023年第6期22卷 1847-1856页

作者： wang yong-li HUANG Chao YU Yang CAI Ri-chun SU yong-chun CHEN Zhi-wu ZHENG Mai-qing CUI Huan-xian State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China Guangxi Jinling Agriculture and Animal Husbandry Group Co. Ltd.Nanning 530049P.R.China

San-Huang chicken is a high-quality breed in China with yellow feather, claw and break. However, the abnormal phenomenon of the yellow shank turning into green shank of San-Huang chicken has been a concern, as it seriously reduces the carcass quality and economic benefit of yellow-feathered broilers. In this study, the cause of this abnormal green skin in shank was systematically investigated. Physiological anatomy revealed that the abnormal skin in shank was primarily due to the deposition of melanin under the dermis. After analyzing multiple potential causes such as heredity(pedigree and genetic markers), environment(water quality monitoring) and feed composition(mycotoxin detection), excessive aflatoxin B1(AFB1) in feed was screened, accompanied with a higher L-dihydroxy-phenylalanine(L-DOPA)(P170 μg kg–1)indeed induced the abnormal green skin in shank compared to the normal AFB1 content(<10 μg kg–1), and the mRNA levels of TYR, TYRP1, MITE, MC1R and EDN3 genes related to melanin deposition would significantly up-regulate(P<0.01) and the content and activity of tyrosinase(TyR) significantly increased(P<0.05). At the same time, the content of L-DOPA and melanin deposition also increased significantly(P<0.01), which also confirmed the effect of excessive AFB1 on melanin deposition in skin of shank. Results of additional experiments revealed that the AFB1's negative effect on melanin deposition in skin of shank could last for a longer time. Taken together, the results of this study explained the occurrence and possible mechanisms of the abnormal AFB1-related green skin in shank of chickens. Excessive AFB1 in diets increased the L-DOPA content and melanin abnormal deposition in the chicken shank possibly via promoting TyR content and activity, and the expression of melanin synthesis-related genes. Furthermor

关键词： aflatoxin B1 melanin deposition skin color in shank chicken negative effect

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Potential regulation of linoleic acid and volatile organic compound contents in meat of chickens by PLCD1

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Journal of Integrative Agriculture 2023年第1期22卷 222-234页

作者： liU Xiao-jing wang yong-li liU li liU Lu ZHAO Gui-ping WEN Jie JIA Ya-xiong CUI Huan-xian State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Omega-3(linolenic acid(ALA),docosapentaenoic acid,eicosapentaenoic acid)and omega-6(linoleic acid(LA),arachidonic acid)polyunsaturated fatty acids are essential for health and normal physiological functioning in humans.Here we report a genome-wide association study(GWAS)on LA content in chicken meat.The 19 significant single nucleotide polymorphisms(SNPs)identified by the GWAS approach were annotated in VILL,PLCD1 and OXSR1 genes with highly polymorphic linkage blocks,and explained 4.5%of the phenotypic variation in the LA content.Specifically,the PLCD1 mRNA expression level was negatively correlated with the LA content,and significantly higher in chickens with low LA content than in those with high LA content.In addition,PLCD1 was found to be involved in metabolic pathways,etc.Furthermore,the LA content was correlated with volatile organic compounds(e.g.,octanal,etc.),but no relationship was found with intramuscular fat and triglycerides in chicken meat.The results indicated that there are key SNPs in PLCD1 that regulate the content of LA,and it has no significant effect on fat deposition,but may affect the content of volatile organic compounds(VOCs).

关键词： linoleic acid flavor quality intramuscular fat PLCD1 chicken meat

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Inhibitive effect of 3-bromopyruvic acid on human breast cancer MCF-7 cells involves cell cycle arrest and apoptotic induction

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Chinese Medical Journal 2009年第14期122卷 1681-1685页

作者： liU Xiao-hong ZHENG Xue-fang wang yong-li Department of Pharmacology Hebei Medical University Shijiazhuang Hebei 050017 China Department of Pharmacy Tangshan Gongren Hospital Tangshan Hebei 063000 China liaoning Key Lab of Bioorganic Chemistry Dalian University Dalian Liaoning 116622 China

Background Breast cancer is one of the most common malignancies in women and is highly resistant to chemotherapy Due to its high tumour selectivity, 3-bromopyruvic acid （3-BrPA）, a well-known inhibitor of energy metabolism has been proposed as a specific anticancer agent. The present study determined the effect of 3-BrPA on proliferation, cell cycle and apoptosis in the human breast cancer MCF-7 cell line and other antitumour mechanisms. Methods MCF-7 cells were treated with various concentrations of 3-BrPA for 1-4 days, and cell growth was measured by 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide assay. Marked morphological changes in MCF-7 cells after treatment with 3-BrPA were observed using transmission electron microscopy. The distributions of the cell cycle and apoptosis were analyzed by flow cytometry. Immunohistochemistry was used to indicate the changes in the expression of Bcl-2, c-Myc, and mutant p53. Results 3-BrPA （25 μg/ml） significantly inhibited the proliferation of MCF-7 cells in a time-dependent manner. The MCF-7 cells exposed to 3-BrPA showed the typical morphological characteristics of apoptosis, including karyopycnosis, nuclear condensation and oversize cytoplasmic particles. In addition, flow cytometric assay also showed more apoptotic cells after 3-BrPA stimulation. The cells at the GO and G1 phases were dramatically decreased while cells at the S and G2/M phases were increased in response to 3-BrPA treatment after 48 hours. Furthermore, 3-BrPA stimulation decreased the expressions of Bcl-2, c-Myc and mutant p53, which were strongly associated with the programmed cell death signal transduction pathway. Conclusion 3-BrPA inhibits proliferation, induces S phase and G2/M phase arrest, and promotes apoptosis in MCF-7 cells, which processes might be mediated by the downregulation of the expressions of Bcl-2, c-Myc and mutant p53.

关键词： apoptosis cell cycle breast cancer 3-bromopyruvic acid

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贸易政策不确定性与企业全要素生产率

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吉林大学社会科学学报 2024年第3期64卷 125-143,238页

作者：王立勇常清胡睿中央财经大学国际经济与贸易学院北京102206 中央财经大学统计与数学学院中邮创业基金管理股份有限公司创新业务部北京100013

在贸易政策不确定性日益加大的背景下,探究贸易政策不确定性与企业全要素生产率之间的因果关系及其影响机制,对于我国建设现代化经济体系和促进经济高质量发展具有重要意义。文章以中国加入WTO后获得美国给予的永久正常贸易关系为准自... 详细信息

在贸易政策不确定性日益加大的背景下,探究贸易政策不确定性与企业全要素生产率之间的因果关系及其影响机制,对于我国建设现代化经济体系和促进经济高质量发展具有重要意义。文章以中国加入WTO后获得美国给予的永久正常贸易关系为准自然实验,借助双重差分模型推断贸易政策不确定性对企业全要素生产率的因果效应,并借助内生转换回归模型和边际处理效应模型对其影响机制进行检验。结果表明,中国加入WTO之后,企业的全要素生产率得到较大幅度提升,企业的全要素生产率随着贸易政策不确定性的下降表现出上升趋势,二者具有因果关系,其中,企业出口行为是重要的影响机制。基于此,持续推进高水平对外开放,积极加强国际合作,维护贸易环境的稳定,应继续作为我国未来经济发展的重要方向。

关键词：贸易政策不确定性全要素生产率准自然实验内生转换回归模型边际处理效应模型

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长短期财政政策不确定性、企业创新决策与全要素生产率

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中央财经大学学报 2024年第5期 3-14页

作者：王立勇杜文会徐晓莉中央财经大学国际经济与贸易学院中央财经大学统计与数学学院北京交通大学经济管理学院

笔者通过构建包含财政政策反应函数的成分GARCH模型测度了长期和短期财政政策不确定性,基于中国A股上市公司的面板数据推断了长期和短期财政政策不确定性对全要素生产率的因果效应,并从理论和实证角度分析内在作用机制。研究发现:长期... 详细信息

笔者通过构建包含财政政策反应函数的成分GARCH模型测度了长期和短期财政政策不确定性,基于中国A股上市公司的面板数据推断了长期和短期财政政策不确定性对全要素生产率的因果效应,并从理论和实证角度分析内在作用机制。研究发现:长期和短期财政政策不确定性对全要素生产率的影响方向不同,长期财政政策不确定性有利于全要素生产率提升,短期财政政策不确定性则降低全要素生产率。其中,企业创新决策是长短期财政政策不确定性影响全要素生产率的重要机制,长期财政政策不确定性鼓励企业选择增加创新的决策,而短期财政政策不确定性导致企业选择推迟创新的决策。短期财政政策不确定性对非国有企业全要素生产率的负面影响更大,而长期财政政策不确定性对不同所有权属性企业全要素生产率的影响无差异。

关键词：长期财政政策不确定性短期财政政策不确定性企业创新决策全要素生产率

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The Common Principal Component Analyses of Multi-RCMs

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Atmospheric and Oceanic Science Letters 2013年第1期6卷 14-20页

作者： FENG Jin-Ming wang yong-li FU Cong-Bin Key Laboratory of Regional Climate-Environment for Temperate East Asia Institute of Atmospheric PhysicsChinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Based on a 10-year simulation of six Regional Climate Models(RCMs) in phase II of the Regional Climate Model Inter-Comparison Project(RMIP) for Asia,the multivariate statistical method of common principal components(CPCs) is used to analyze and compare the spatiotemporal characteristics of temperature and precipitation simulated by multi-RCMs over China,including the mean climate states and their seasonal transition,the spatial distribution of interannual variability,and the interannual variation.CPC is an effective statistical tool for analyzing the results of different models.Compared with traditional statistical methods,CPC analyses provide a more complete statistical picture for observation and simulation results.The results of CPC analyses show that the climatological means and the characteristics of seasonal transition over China can be accurately simulated by RCMs.However,large biases exist in the interannual variation in certain years or for individual models.

关键词：主成分分析区域气候模型多元统计方法中国共产党年际变化区域气候模拟模式模拟 CPCS

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Simulation of Extreme Climate Events over China with Different Regional Climate Models

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Atmospheric and Oceanic Science Letters 2011年第1期4卷 47-56页

作者： FENG Jin-Ming wang yong-li FU Cong-Bin Key Laboratory of Regional Climate-Environment for East Asia Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

During phase Ⅱof the Regional Climate Model Inter-comparison Project (RMIP) for Asia,the Asian climate was estimated from July 1988 to December 1998 using six climate models.In this paper,the abilities of six climate models to simulate several important extreme climate events in China during the last years of the last century were analyzed.The modeled results for the intensity of the precipitation anomaly over the Yangtze-Huaihe Valley during the summers of 1991 and 1998 were weaker than the observed values.The positive precipitation anomaly responsible for a catastrophic flood in 1991 was well reproduced in almost all simulation results,but the intensity and range of the precipitation anomaly in 1998 were weaker in the modeled results.The spatial distribution of extreme climate events in 1997,when severe drought affected North China and flood impacted South China,was reproduced by most of the regional models because the anomaly of the large-scale background field was well-simulated,despite poor simulation of high temperature areas in the north during the summer by all models.

关键词：极端气候事件模拟能力中国南方气候模型区域气候模式异常强度降水异常中国北方

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Comprehensive profiling and characterization of the absorbed components and metabolites in mice serum and tissues following oral administration of Qing-Fei-Pai-Du decoction by UHPLC-Q-Exactive-Orbitrap HRMS

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Chinese Journal of Natural Medicines 2021年第4期19卷 305-320页

作者： liU Wei HUANG Jian ZHANG Feng ZHANG Cong-Cong li Rong-Sheng wang yong-li wang Chao-Ran liANG Xin-Miao ZHANG Wei-Dong YANG ling liU Ping GE Guang-Bo Key Laboratory of liver and Kidney Diseases(Ministry of Education) Institute of Liver DiseasesShanghai Key Laboratory of Traditional Chinese Clinical MedicineShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 201203China Institute of Interdisciplinary Integrative Medicine Research Shanghai University of Traditional Chinese MedicineShanghai 201203China Institute of Chinese Materia Medica Shanghai University of Traditional Chinese MedicineShanghai 201203China Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023China

Qing-Fei-Pai-Du decoction(QFPDD)is a Chinese medicine compound formula recommended for combating corona virus disease 2019(COVID-19)by National Health Commission of the People's Republic of China.The latest clinical study showed that early treatment with QFPDD was associated with favorable outcomes for patient recovery,viral shedding,hospital stay,and course of the disease.However,the effective constituents of QFPDD remain unclear.In this study,an UHPLC-Q-Orbitrap HRMS based method was developed to identify the chemical constituents in QFPDD and the absorbed prototypes as well as the metabolites in mice serum and tissues following oral administration of QFPDD.A total of 405 chemicals,including 40 kinds of alkaloids,162 kinds of flavonoids,44 kinds of organic acids,71 kinds of triterpene saponins and 88 kinds of other compounds in the water extract of QFPDD were tentatively identified via comparison with the retention times and MS/MS spectra of the standards or refereed by literature.With the help of the standards and in vitro metabolites,195 chemical components(including 104 prototypes and 91 metabolites)were identified in mice serum after oral administration of QFPDD.In addition,165,177,112,120,44,53 constituents were identified in the lung,liver,heart,kidney,brain,and spleen of QFPDD-treated mice,respectively.These findings provided key information and guidance for further investigation on the pharmacologically active substances and clinical applications of QFPDD.

关键词： Qing-Fei-Pai-Du decoction(QFPDD) Corona virus disease 2019(COVID-19) Chemical profiling Absorbed components High-Resolution Mass Spectrometry(HRMS)

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Individualized treatment of ruptured intracranial aneurysms by microsurgical clipping and endovascular coiling： results from a consecutive series of 212 patients

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Chinese Medical Journal 2012年第19期125卷 3584-3586页

作者： liU Bing WEI Wei wang yong-li YANG Xin-yu ZHU Tao YUE Shu-yuan ZHANG Jian-ning Department of Neurosurgery Tianjin Medical University General HospitalTianjin Neurological InstitutionTianjin 300052China

Subarachnoid hemorrhage （SAH） is a subtype of stroke that is most commonly caused by the rupture of intracranial aneurysm. Endovascular occlusion of intracranial aneurysms has emerged as an alternative to the established microsurgical clipping, The decision process when choosing a treatment for ruptured intracranial aneurysm should comprise an algorithm that was based on the characteristics of the aneurysm and patient status, age, and neurological condition, rather than a blanket assumption that one modality is superior to another.

关键词： intracranial aneurysm clipping coiling subarachnoid hemorrhage

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LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer

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Biomedical and Environmental Sciences 2020年第11期33卷 849-856页

作者： ZENG Yi SI yong Feng LAN Gui Ping wang Zhan ZHOU ling TANG Min Zhong SJ O`Brien LAN Jiao ZHOU Xiang Yang wang yong li TANG Juan ZHOU Zhi Xiang DU Hai Jun liN Hui State Key Laboratory for Infectious Disease Prevention and Control National Institute of Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China People's Hospital of Guangxi Zhuang Autonomous Region Nanning 530021GuangxiChina Cancer center Wuzhou Red Cross HospitalWuzhou 543002GuangxiChina Guy Harvey Oceanographic Center Halmos College of Natural Sciences and OceanographyNova Southeastern UniversityFlorida 33004USA College of life Science and Bioengineering Beijing University of TechnologyBeijing 100124China

Objective To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2(LMP2)modified dendritic cells(DCs)that boosts specific responses of cytotoxic T lymphocytes(CTLs)to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma(NPC).Methods DCs were derived from peripheral blood monocytes of patients with NPC.We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2(rAd-LMP2).NPC patients were immunized with 2×105 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks.Specific responses to LMP2 were detected by enzyme-linked immunospot(EliSPOT)assay at week 0 and at the fifth and eighth weeks.Local clinicians performed the follow-up and tracking of patients.Results We demonstrated that DCs derived from monocytes displayed typical DC morphologies;the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay.Twenty-nine patients with NPC were enrolled in this clinical trial.The LMP2-DCs vaccine was well tolerated in all of the patients.Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC.The follow-up data of 29 immunized patients from April,2010 to April 2015 indicated a five-year survival rate of 94.4%in responders and 45.5%in non-responders.Conclusion In this pilot study,we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC.Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC,which warrants further clinical testing.

关键词： LMP2-DCs Nasopharyngeal carcinoma Immunotherapy

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