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Spatial patterns of interprovincial mountain geographical names in China and implications for regional governance

Journal of Mountain Science 2018年第11期15卷 2517-2530页

作者： ZHANG Sheng-rui wang ying-jie JU Hong-run ZHANG Tong-yan LI Dai-chao FANG Lei wang ying-ying Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China State Key Laboratory of Resources and Environmental Information Systems Institute of Geographic and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China School of Tourism and Geography Science Qingdao University Oingdao 266071 China

Suffering from fragile environment, poor accessibility and infrastructure, as well as social,political and economic marginality, the interprovincial mountain geographical entities are difficult areas for the regional governance in *** analyzing the spatial patterns and the influencing factors of the interprovincial mountain geographical names(IMGNs), the goal of this research is to clarify the geographical features of IMGNs and offer alternatives for the management of interprovincial mountain regions in China. The spatial visualization,the analysis of spatial agglomeration and the influencing factors of IMGNs were all implemented under a geographical information system. Results showed that there were 6869 IMGNs in China according to the database of "China's Second National Survey of Geographical Names(2014-2018)",including 4209 mountain geographical names, 1684 mountain peak geographical names and 976 the other mountain geographical names. Hunan Province had the largest number of names while Shanghai had the smallest number of names. In addition, the spatial variance of the mountain peak names and the mountain names were larger than that of the other mountain geographical names, and the IMGNs showed a significant clustering phenomenon in the southern part of China. The relative elevation and the population had an impact on the distribution of the IMGNs. The largest number of the names occurred where the relative elevation was between 1000-2000 m and where the population was between 40-50 million. Density of unnamed interprovincial mountain geographical entities declined from west to east in China. The analysis of generic names of different types of IMGNs implied that the naming of IMGNs is inconsistent. Based on these analyses, it is suggested that the government should take the IMGNs as management units, strengthen the naming of unnamed interprovincial mountain geographical entities, standardize the generic names of IMGNs and identify areas of poverty based on

关键词： Interprovincial mountain geographical names Spatial association Spatial variance GIS Regional governance China

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NUMERICAL SIMULATION OF ARTIFICIAL VENTILATED CAVITY

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Journal of Hydrodynamics 2006年第3期18卷 273-279页

作者： JIA Li-ping wang Cong WEI ying-jie wang Hai-bin ZHANG Jia-zhong YU Kai-ping School of Astronautics Harbin Instituteof Technology Harbin 150001 China

In order to determine artificial ventilated cavity shape and provide design reference for engineering applications, the shape of ventilated cavity and the drag of underwater body have been numerically simulated with commercial code in this work. Empirical formulas between ventilated cavity shape and cavitation number are obtained under the conditions of zero incidence and similarity shapes of ventilated cavity and vapor cavity have been validated under the same cavitation number. In addition, the relations between change of cavitator incidence and cavity unsymmetry and the relations of between drag and cavity shape have been primarily analysed. Furthermore, the numerical results fit well with the experimental results.

关键词： ventilated cavity numerical simulation,cavity shape

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Simplified isolation and spheroidal aggregate culture of rat hepatocytes

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World Journal of Gastroenterology 1998年第1期4卷 79-81页

作者： wang ying-jie LI Meng-Dong wang Yu-Min DING Jian NIE Qing-He 不详

IM To explore a simplified method for isolation of hepatocytes and establish a method of primary hepatocyte culture with more aggregates and longer persisence.METHODS Wistar rat hepatocytes were isolated by a single extracorporeal twostep perfusion method, and the cells were seeded on polyHEMA coated flasks and cultured with hormonally defined medium and gentle shaking at regular intervals.RESULTS The total yield of isolating hepatocytes amounted to 108 cells for each rat liver with the viability of more than 90% in all isolations. Under the nonadherent environments, the cells were found to attach to each other and form multicellular aggregates rapidly, and the aggregates became spheroidal shape after two days in culture. The morphologic characteristics and albumin synthetic function of the multicellular spheroidal aggregates can be maintained for one month.CONCLUSION The simple and reliable isolation as well as large scale and longer time culture of hepatocytes can be used for experiments in liver cell transplantation and bioartificial liver support system.

关键词： liver cell cell isolation tissue culure aggregates

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The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

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Journal of Integrative Agriculture 2020年第12期19卷 3054-3064页

作者： ZHANG Ya-ni HU Cai wang ying-jie ZUO Qi-sheng LI Bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China

Lin28A and Lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression and regulation of Lin28A have been well documented, but such information for Lin28B is limited. In this study, a fragment of the Lin28B promoter was cloned, the pEGFP-pLin28B vector was constructed. DF-1 chicken fibroblasts were transfected and the expression of green fluorescent protein (GFP) was measured. Furtherly, Lin28B promoter of different lengths fragments was cloned using the chromosome-walking method and the fragments were ligated into the PGL3-Basic vector, and transfected into DF-1 cells. Results of dual-luciferase reporter assay showed that the core of the Lin28B promoter was included in the sequence from –1 431 to –1 034 bp. The binding sites of the transcription factor TCF7L2 was showed within this sequence by bioinformatics analysis. The promoter activity of Lin28B was downregulated (Pying Lin28B expression.

关键词： primordial germ cells Lin28B promoter H3K4me2 Wnt5a/β-catenin/TCF7L2

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Study on the transverse structures across Taiwan Strait

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Science China Earth Sciences 2002年第12期45卷 1114-1126页

作者： MA Zongjin (马宗晋) wang Chien-ying (王乾盈) XU jie (徐杰) NIE Fengjun (聂逢君) ZHANG Jin (张进) Institute of Geology China Seismological Bureau Beijing 100029 China Institute of Geophysics National Central University Taibei 32054 China Institute of Geology China Seismological Bureau Beijing 100029 China Institute of Geology China Seismological Bureau Beijing 100029 China Institute of Geology China Seismological Bureau Beijing 100029 China

The mountain ranges are almost NE- or NNE-trending in mainland and Taiwan island across the strait. But a number of correspondent transverse structures across Taiwan Strait have been identified on the basis of morphology, surface structure, deep seismic survey and gravity field etc. Moreover, it is inferred from the square or/and rectangular shape of the isoseist maps of 8 M≥6 earthquakes occurring in the western foothill plain of Taiwan that there is an apparent segmentation of the Taiwan Island along the N-S orientation. This has been verified from the GPS's results of the ground motions measured during the Chi-Chi earthquake in 1999. As a matter of fact, the transverse structures within the Taiwan Island could be correlated with more salient NW- and EW-trending structures in the mainland of Fujian than in Taiwan. The investigation of the transverse structures across Taiwan Strait is conducive to realizing the geology within the strait. Hence, the tectonic sketch map of the strait as well as mainland and island drawn in this context shows NW- and EW-trending structures for the study area.

关键词： Taiwan Strait, transverse structure, Chi-Chi earthquake, GPS.

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Chinese herbal formulae defend against Mycoplasma gallisepticum infection

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Journal of Integrative Agriculture 2022年第10期21卷 3026-3036页

作者： wang ying-jie LIANG Ya-xi HU Fu-li SUN ying-fei ZOU Meng-yun LUO Rong-long PENG Xiu-li Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of EducationHuazhong Agricultural UniversityWuhan 430070P.R.China

Mycoplasma gallisepticum HS strain(MG-HS)is a pathogen that causes chronic respiratory disease(CRD)in chicken,which is characterized by host respiratory inflammatory damage,brings huge economic losses to the poultry ***,emerging Chinese herbal medicines(CHM)have been used to treat *** study was aimed to investigate the preventive and therapeutic effects and their potential mechanisms of Chinese herbal medicinal formulae(CHMF),which consisted of 10 kinds of Chinese herbal medicine including Scutellaria,Houttuynia cordate and licorice,on MGinduced CRD in *** respect to the preventive effect,the results showed that CHMF could effectively recover the MG-induced decrease on body weight and feed conversion *** analysis showed that both prevention and treatment of CHMF could significantly alleviate the severe respiratory inflammation induced by MG ***,compared with the MG infection group,both the prevention groups and the treatment groups of CHMF could effectively reduce the expression of MG adhesion protein(p MGA1.2)to inhibit the proliferation of MG,and thus effectively inhibit the expression of MG-induced inflammatory factors interleukin-1β(IL-1β)and tumour necrosis factor-α(TNF-α).In summary,these findings confirm that CHMF can protect chickens from various tissue damage caused by MG infection and has no adverse effects on the performance of chickens in the short *** its efficacy against MG is equal to or better than that of tiamulin.

关键词： Mycoplasma gallisepticum HS strain(MG-HS) Chinese herbal medicine formulae production performance inflammatory lesions chicken

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Identification of a novel gain-of-function mutant allele,slr1-d5,of rice DELLA protein

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Journal of Integrative Agriculture 2016年第7期15卷 1441-1448页

作者： ZHANG Yun-hui BIAN Xiao-feng ZHANG Suo-bing LING Jing wang ying-jie WEI Xiao-ying FANG Xian-wen The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm/Jiangsu Provincial Key Laboratory of Agrobiology/Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Shandong Business Institute Yantai 264670P.R.China

Controling the height of crops plays a crucial role for their yields. The large scale utilization of semi-dwarf varieties has greatly improved crop yield, providing an effective support for world food security. In rice, a main food for over half of the world’s population, a number of dwarf loci have been identiifed. However, most of them are recessive, such as the ‘green revolution’ genesd1. To gain more beneifcial loci for rice breeding programs, exploring new mutations is needed, especialy the dominant loci which can be used broadly for hybrid breeding. Here, we isolated a novel dominant dwarf rice mutant, slr1-d5. Al of the internodes ofslr1-d5are reduced. We ifnd that the responsiveness ofslr1-d5to gibberelin （GA）, GA3, was signiifcantly reduced. Map-based cloning revealed that the dominant dwarifsm ofslr1-d5was caused by an amino acid substitution in the N-terminal TVHYNP domain of rice DELLA protein, SLR1, where the conserved amino acid Pro （P） was substituted to His （H）. Our ifndings not only further prove the pivotal role of TVHYNP motif in regulating SLR1 stability, but also provide a new dwarf source for improvement of rice germplasms.

关键词： rice dominant dwarf DELLA protein gibberelin

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Experimental study of bioartificial liver with cultured human liver cells

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World Journal of Gastroenterology 1999年第2期5卷 47-49页

作者： wang ying jie, LI Meng Dong, wang Yu Ming, NIE Qing He and CHEN Guo Zheng

AIM To establish an extracorporeal bioartificial liver support system (EBLSS) using cultured human liver cells and to study its support effect for fulminant hepatic failure (FHF).METHODS The liver support experiment of EBLSS consisting of aggregates cultured human liver cells, hollow fiber bioreactor, and circulation unit was carried out in dizhepatic *** The viability of isolated hepatocytes and nonparenchymal liver cells reached 96%. These cells were successfully cultured as multicellular spheroids with synthetic technique. The typical morphological appearance was retained up to the end of the artificial liver experiment. Compared with the control dogs treated with EBLSS without liver cells, the survival time of artificial liver support dogs was significantly prolonged. The changes of blood pressure, heart rate and ECG were slow. Both serum ammonia and lactate levels were significantly lowered at the 3rd h and 5th h. In addition, a good viability of human liver cells was noted after 5 h *** EBLSS playing a metabolic role of cultured human hepatocytes, is capable of compensating the function of the liver, and could provide effective artificial liver support and therapy for patients with FHF.

关键词： artificial liver cell, cultured hepatocytes fulminant hepatic failure animal model liver support

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透平叶栅模化时粗糙度和雷诺数对性能的影响及修正方法

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工程热物理学报 2022年第1期43卷 91-97页

作者：王英杰戴韧上海理工大学能源与动力工程学院上海200093

基于平板边界层理论和对流换热的雷诺比拟关系,通过粗糙度和雷诺数对表面摩擦因子f的影响规律,建立了透平叶栅模化实验的叶型损失系数ω和表面平均换热系数h^(-)与f之间的修正关联式。对比模化叶型与实验叶型的数值分析结果,在选定参数... 详细信息

基于平板边界层理论和对流换热的雷诺比拟关系,通过粗糙度和雷诺数对表面摩擦因子f的影响规律,建立了透平叶栅模化实验的叶型损失系数ω和表面平均换热系数h^(-)与f之间的修正关联式。对比模化叶型与实验叶型的数值分析结果,在选定参数范围内,ω和h^(-)与f之间呈现良好的相关性,采用近似线性关系修正实验模型的叶型损失和平均表面换热系数,与模化叶型实际性能之间的误差均可控制在10%以内。

关键词：透平叶栅模化实验性能修正表面粗糙度

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Research on the appropriate way to transfer exogenous substances into chicken embryos

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Journal of Integrative Agriculture 2017年第10期16卷 2257-2263页

作者： wang Yi-lin JIN Kai HE Na-na CHENG Shao-ze ZUO Qi-sheng LI Dong wang ying-jie wang Fei JI Yan-qing LU Zhen-yu ZHANG Chen wang Man ZHAO Rui-feng YU Xin-jian ZHANG Ya-ni ZHAO Wen-ming LI Bi-chun College of Animal Science and Technology/Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design Yangzhou University Yangzhou 225009 P.R.China

In biological research, chicken embryos are a classic experimental model for the exploration of the embryonic development and cell differentiation. Transferring exogenous substances into chicken embryos for producing medical antibodies has been widely used in the production practice. However, there are few studies about the effect of the different injection site and dosage on chicken embryos. The aim of this study was to explore the effects of different injection sites and dosages on chicken embryo hatching rate and development, so as to provide a basis for further studies using the chicken embryo model. Freshly laid eggs （Rugao yellow chicken） were injected with different doses of saline at the tip, equatorial plane and the blunt end of the egg shell, respectively. Egg hatching rate was recorded and compared among injection sites and different doses. A trypan blue stain was also injected at the aforementioned sites and the growth of chicken embryos was observed. The SPSS （statistical package for the social science） software was used to analyze the relationship between the chicken eggs hatching rate and the different injection sites or the different dosages. The experimental results showed that there were significant differences on egg hatching rates among the different injection sites and doses （P〈0.05）. The hatchability of the blunt end injection group was significantly higher than that of the other two sites. The egg hatching rate decreased with increased saline doses. The egg hatching rate of the 100 pL saline injection group was higher than the 200 and 300 μL dosage groups. Ultimately, we suggest that the optimal chicken embryo injection process is during early development, at the blunt end site with a dose less than 100 μL to minimize damage to the egg.

关键词： chicken embryos injection dosage hatchability chick embryo development

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