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Communications in Theoretical Physics 2008年第8期50卷 299-302页

作者： wang yi-Hong wang sheng-Kui Department of Mathematics Zhejiang Foresty University Hangzhou 311300 China

In this paper,with the aid of symbolic computation,we present a uniform method for constructing soliton solutions and periodic solutions to (2+1)-dimensional Toda lattice equation.

关键词： soliton solutions periodic solutions (2+l)-dimensional differential-difference system

Effect of insulin on functional status of cord blood-derived dendritic cells and on dendritic cell-induced CTL cytotoxicity against pancreatic cancer cell lines

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Hepatobiliary & Pancreatic Diseases International 2009年第5期8卷 529-534页

作者： Liu, Qiu-Liang wang, yi-sheng wang, Jia-Xiang Zhengzhou Univ Dept Surg Affiliated Hosp 1 Zhengzhou 450052 Peoples R China

BACKGROUND: Dendritic cells (DCs) are the most important antigen-presenting cells in the human body, and DCs with different mature status possess different or even opposite functions. This study was designed to explore the influence of insulin on the functional status of cord blood-derived DCs and on DC-induced cytotoxic T lymphocyte (CTL) activity against pancreatic cancer cell lines. METHODS: Mononuclear cells were isolated from fresh cord blood. Interleukin-4 (IL-4) and granulocytemacrophage colony-stimulating factor (GM-CSF) were used to induce or stimulate the mononuclear cells. Insulin at different concentrations served to modify DCs, and then DC morphology, number, and growth status were assessed. The DC immunophenotype was detected with a flow cytometer. The IL-12 in DC supernatant was determined by ELISA. DC functional status was evaluated by the autologous mixed lymphocyte reaction. T lymphocytes were induced by insulin-modified DCs to become CTLs. The CTL cytotoxicity against pancreatic cancer cell lines was determined. RESULTS: Mononuclear cells from cord blood can be differentiated into DCs by cytokine induction and insulin modification. With the increase in insulin concentration (2.5-25 mg/L), the expression of DC HLA-DR, CD1 alpha, CD80, and CD83 was significantly increased, the DC ability to secrete IL-12 was significantly improved, DC function to activate autologous lymphocytes was significantly enhanced, and the cytotoxicity of CTLs induced by insulin-modified DCs against pancreatic cancer cell lines was significantly strengthened. CONCLUSIONS: Insulin may facilitate DC induction and maturation, and improve the reproductive activity of autologous lymphocytes. The cytotoxicity of CTLs induced by insulin-modified DCs against pancreatic cancer cell lines was significantly enhanced. Insulin may serve as a factor modifying DCs and inducing CTLs in vitro in insulin biotherapy.

关键词： insulin cord blood dendritic cell pancreatic cancer

Acoustic impedance characteristics of linear compressors

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第7期14卷 494-503页

作者： Zhi-hua GAN Long-yi wang sheng-ying ZHAO Yu-jing SONG Wei-wei wang yi-nong WU Institute of Cryogenics and Refrigeration Zhejiang University School of Information and Electrical Engineering Zhejiang University City College Shanghai Institute of Technical Physics Chinese Academy of Sciences

The acoustic field of a linear compressor serves to deliver the compression work to the load, such as the connected cold head of a cryocooler; it plays an equivalently important role as the electrical and mechanical parts, especially in the impedance match issue. This paper studies the acoustic impedance characteristics of a linear compressor. The parameters including the current, the piston displacement, the pressure amplitude, the electrical power dissipation, the power factor, the pressure-volumetric (PV) power delivered, and the efficiency are theoretically and experimentally investigated. Different from previous theoretical studies, optimization for the operations away from the resonance is also included. More general optimization results imply relevance between thermoacoustic engines and linear compressors. The predicted results are validated by the experiments performed on a linear compressor with an adjustable resistive-capacitive (RC) acoustic load. The comparisons between the calculations and the measurements are presented and analyzed. The results provide deeper insight into the mechanism of the linear compressor and the impedance match in a cryocooler system.

关键词： Linear compressor Acoustic impedance Resistive-capacitive (RC) load

REAL PIECEWISE ALGEBRAIC VARIETY

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Journal of Computational Mathematics 2003年第4期21卷 473-480页

作者： Ren-hong wang yi-sheng Lai (Institute of Mathematical sciences, Dalian University of Technology, Dalian 116024, China) Institute of Mathematical sciences Dalian University of Technology Dalian 116024 China

We give definitions of real piecewise algebraic variety and its dimension. By using the techniques of real radical ideal, P-radical ideal, affine Hilbert polynomial, Bernstein-net form of polynomials on simplex, and decomposition of semi-algebraic set, etc., we deal with the dimension of the real piecewise algebraic variety and real Nullstellensatz in Cμ spline ring.

关键词： Dimension Real piecewise algebraic variety Cμ spline ring.

The Estimation of Soil Trace Elements Distribution and Soil-Plant-Animal Continuum in Relation to Trace Elements Status of Sheep in Huangcheng Area of Qilian Mountain Grassland,China

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Journal of Integrative Agriculture 2014年第1期13卷 140-147页

作者： wang Hui LIU Yong-ming QI Zhi-ming wang sheng-yi LIU Shi-xiang LI Xia wang Hai-jun wang Xiao-li XIA Xin-chao ZHU Xin-qiang Key Laboratory of Veterinary Pharmaceutics Development Ministry of Agriculture/Key Laboratory of New Animal Drug Project of Gansu Province/Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutics SciencesChinese Academy of Agricultural Sciences

The purpose of the present study was to survey contents of trace elements of Cu, Mn, Fe, and Zn in the surface layer （0-20 cm） in the soil, pasture and serum of sheep in Huangcheng area of Qilian mountain grassland, China. Also the soil-plantanimal continuum was analyzed. Soil （n=300）, pasture （n=60）, and blood serum samples from sheep （n=480） were collected from Huangcheng area of Qilian mountain grassland, China. The contents of trace element in the samples were analyzed by atomic absorption spectrophotometer after digestion. The soil trace elements density distribution shows a ladder-like pattern distribution. Equations developed in the present study for prediction of Fe （R2=0.943） and Zn （R2=0.882） had significant R2 values.

关键词： trace elements minerals deficiency soil type Qilian mountain grassland correlation

Effect of growth hormone on the immune function of dendritic cells

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Chinese Medical Journal 2010年第8期123卷 1078-1083页

作者： LIU Qiu-liang wang yi-sheng wang Jia-xiang Department of Surgery the First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China

Background Dendritic cells （DCs） are one of the most important antigen presenting cells in the human body, and DCs at various stages of maturation possess different or even opposite functions. The aim of this study was to investigate the influence of growth hormones on the functional status of cord blood-derived DCs encompassing immunophenotype, ability to excrete interleukin （IL）-12 and provoke autologous leukomonocyte. Methods Mononuclear cells were isolated from fresh cord blood, with IL-4 and granulocyte-macrophage colony-stimulating factor （GM-CSF） used to induce and stimulate the mononuclear cells. Growth hormone at different concentrations was used to modify DCs, and then DCs morphology, number and growth status were observed. The immunophenotype of DCs was detected with a flow cytometer. The concentration of IL-12 in the DCs supematant was determined by enzyme linked immunosorbent assay （ELISA） and DCs functional status was evaluated by autologous mixed lymphocyte reactions. Results Mononuclear cells from cord blood can be differentiated into DCs by cytokine induction and growth hormone modification. With the increase in growth hormone concentrations （5-100 pg/L）, the expression of DCs HLA-DR, CDla, CD80 and CD83 were significantly increased （P 〈0.05）. The ability of DCs to secrete IL-12 was significantly improved （P 〈0.05）, and the ability of DCs to activate autologous lymphocytes was significantly enhanced （P 〈0.05）. Pegvisomant was able to ablate the effects of growth hormone on DCs. Conclusions Growth hormone may facilitate DCs induction and maturation, and improve the reproductive activity of autologous lymphocytes in a dose-dependent manner. Growth hormone may serve as a factor of modifying DCs to achieving maturity.

关键词： dendritic cells growth hormone immune pegvisomant

Dexamethasone-induced adipogenesis in primary marrow stromal cell cultures: mechanism of steroid-induced osteonecrosis

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Chinese Medical Journal 2006年第7期119卷 581-588页

作者： yiN Li LI Yue-bai wang yi-sheng Department of Orthopedics First Affiliated Hospital of ZhengzhouUniversity Zhengzhou 450052 China Department of Biochemistry Medical College of ZhengzhouUniversity Zhengzhou 450052 China

Background In steroid-induced osteonecrosis, hypertrophy and hyperplasia of marrow fat cells and lipid deposition of osteocytes can be found in the femoral head. However, the precise reason is not clear yet. The aim of this study was to observe the effect of dexamethasone （Dex） on differentiation of marrow stromal cells （MSCs）, and to investigate the pathobiological mechanism of steroid-induced osteonecrosis.Methods MSCs in cultures were treated with increasing concentrations of Dex （0, 10^-9, 10^-8, 10^-7, and 10^-6 mol/L） continuously for 21 days. The cells, which were exposed to 0 mol/L （control） or 10^-7 mol/L Dex for 4-21 days, were then cultured for 21 days without Dex. MSCs were stained with Sudan Ⅲ. Number of adipocytes was counted under a light microscope. The activity of alkaline phosphatase （ALP） of MSCs treated with 0, 10^-8, 10-7, and 10^-6 mol/L Dex for 12 days, and that treated with 0 mol/L and 10^-7 mol/L Dex for 8, 10, or 12 days were determined. The levels of triglycerides, osteocalcin and cell proliferation of MSCs treated with 0 mol/L and 10^-7 mol/L Dex were detected. The mRNA expression levels of adipose-specific 422（aP2） gene and osteogenic gene type I collagen in MSCs treated with 0 mol/L and 10^-7 mol/L Dex for 6 days were analyzed by whole-cell dot-blot hybridization. Statistical analysis was performed using Student＇s t test and analysis of variance. P values less than 0.05 were considered significant statistically.Results The number of adipocytes in cultures increased with the duration of MSCs＇ exposure to Dex and the concentration of Dex. The level of ALP activity in the MSCs decreased with concentration of Dex. In the control group, it was 8.69 times of that in the 10^-7 mol/L Dex group on day 12 （t=20.51, P〈0.001）. The level of triglycerides in 10^-7 mol/L Dex group was 3.40 times of that in the control （t=11.00, P〈0.001）. The levels of cell proliferation and osteocalcin in the control were 1.54 and 2.42 times of that in the 10^-7 m

关键词： Dexamethasone bone marrow adipocytes osteonecrosis

Titanium mesh fusion device in the treatment of thoracolumbar burst fracture

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Chinese Medical Journal 2007年第3期120卷 246-247页

作者： wang yi-sheng yiN Li BAO Heng wang Wei-dong Department of Orthopedics First Affiliated Hospital of Zhengzhou University. Zhengzhou 450052 China

Thoracolumbar burst fracture, a common condition in clinic, often leads to severe spinal instability and neurologic deficit. In most cases, the compression that caused by backward protrusion of the fracture fragments to the spinal cord, makes complete decompression difficult through a posterior approach. Here, we reviewed the clinical records of 22 patients with thoracolumbar burst fracture treated by anterior corpectomy, decompression of the spinal cord, and implantation of titanium mesh cage.

关键词： thoracolumbar burst fracture titanium mesh fusion divice surgery

Osteogenic potential of human calcitonin gene-related peptide alpha gene-modified bone marrow mesenchymal stem cells

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Chinese Medical Journal 2011年第23期124卷 3976-3981页

作者： wang yi-sheng wang Ya-han ZHAO Guo-qiang LI Yue-bai Department of Orthopaedics the First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China

Background Most of the basic and clinical studies of osteonecrosis of the femoral head （ONFH） are restricted to bone tissues only, whereas various systems are involved in the onset and development of ONFH, including nervous system. Peptidergic nerve participates in the neuronal regulation of bone metabolism and anabolism, and plays key roles in the growth, repair and reconstruction of bone. Calcitonin gene-related peptide （CGRP）, which is secreted by peptidergic nerve, is the main mediator of bone metabolism. It dramatically promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells （BMSCs）. Additionally, it enhances the osteoblast mass and the rate of osteoblast formation, and reduces the bone resorption by acting on osteoblasts and osteoclasts. Hence, we aimed to construct recombinant retrovirus vector pLNCX2-hCGRPα and to investigate the proliferation and osteogenic potential of hCGRPα-producing BMSCs （BMSCs/pLNCX2-hCGRPα） after virus infection. Methods The constructed recombinant retrovirus vector pLNCX2-hCGRPα was transfected into PT67 packaging cells by lipofectamine 2000. Virus was collected for BMSCs infection. The mRNA and protein expression of hCGRPα was examined by reverse transcription polymerase chain reaction （RT-PCR） and Western blotting, respectively. The cell proliferation was determined by methyl thiazoleterazolium （MTT） assay. The osteogenic potential of BMSCs was evaluated by alkaline phosphatase （ALP） activity. Results Both mRNA and protein expression of hCGRPα was detected in BMSCs/pLNCX2-hCGRPα cells. These cells exhibited significantly elevated proliferation and ALP value as compared with control BMSCs （P 〈0.05）. Conclusion BMSCs/pLNCX2-hCGRPα cells could stably express hCGRPα and showed promoted proliferation ability and osteogenic potential as compared with control BMSCs.

关键词： retrovirus calcitonin gene-related peptide gene recombinant bone marrow mesenchymal stem cells osteonecrosis of the femoral head

Construction and identification of the recombinant adenovirus vector carrying a small interfering RNA targeting the peroxisome proliferator-activated receptor-γ

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Chinese Medical Journal 2012年第4期125卷 671-675页

作者： LIU Ming wang yi-sheng LI Yue-bai ZHAO Guo-qiang Department of Orthopedic Surgery First Affiliated Hospital of Zhengzhou University Zhengzhou Henan 450052 China Basic Medical College Zhengzhou University Zhengzhou Henan 450001 China

Background Steroid-induced osteonecrosis of the femoral head （ONFH） is a common clinical disease,with a high disability *** present,efficient prevention and treatment of steroid-induced ONFH is still *** peroxisome proliferator-activated receptor-γ （PPARγ） is recognized as an important pathogenic gene for the development of steroid-induced *** interference （RNAi） is a tool for functional gene analysis,which has been successfully used to down-regulate the levels of specific target ***,down-regulation of PPARγ expression by RNAi may prevent the incidence of steroid-induced *** According to the principles of siRNA design,three duplex siRNA sequences （971-989,1253-1271 and 1367-1385） derived from the PPARy gene （NM_001082148） were *** duplexes were annealed,purified and ligated into 1.0-cytomegalovirus （CMV） shuttle *** shuttle vector was transfected into HEK293 *** HEK293 generated recombinant adenovirus vector carrying PPARγ siRNA sequences was purified and the titer of recombinant adenovirus was *** After the annealing of single-strand DNA oligo encoding short hairpin RNA （shRNA） sequences,products were identified by gel *** products were ligated into the 1.0-CMV shuttle vector and the recombinant shuttle vectors 1.0-CMV-971,1.0-CMV-1253 and 1.0-CMV-1367 were *** sequences of these recombinant vectors were *** then successfully constructed the recombinant adenovirus vector carrying siRNA targeting PPARγ.After purification,the virus titer was higher than 1010 plaque forming unit （PFU）/*** In this study,three recombinant adenovirus shuttle vectors carrying siRNA targeting PPARγ,including shuttle vectors 1.0-CMV-971,1.0-CMV-1253 and 1.0-CMV-1367,were successfully constructed and high titers of recombinant adenovirus were obtained.

关键词： RNA interference peroxisome proliferator-activated receptor-γ adenovirus vector clone