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Anti-hyperalgesia of spinal neurotrophin-3 in CFA induced monoarthritic rats

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Anti-hyperalgesia of spinal neurotrophin-3 in CFA induced mo...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Wen-Li MI,Qi-Liang Mao-YING,Qiong LIU,Xiao-Wei wang,Xiu LI,Chang-Jiang yanG,yan-Qing wang*,Gen-Cheng WU~* Department of Integrative Medicine and Neurobiology,shanghai Medical college,fudan university,shanghai 200032, china

＜正＞Arthritis is a painful and disabling disease that affects millions of *** studies reported beneficial actions of neurotrophin-3（NT-3） related to neuropathic pain,but there are still inconsistent *** the role of NT-3 in the nociceptive signaling needs to be further *** an inflammatory pain model induced by intra-articular injection of complete Freund’s adjuvant（CFA） in rats,the present study demonstrated that（1） the expression of NT-3 mRNA and protein significantly increased in spinal dorsal horn of rats after CFA injection;（2） antisense specifically against NT-3 intrathecally administered 3 days after CFA injection for 7 days,significantly aggravated thermal hyperalgesia induced by CFA injection,compared to the NS or sense treatment;（3） knockdown of NT-3 remarkably increased the expression of GFAP,CD11b and proinflammatory cytokines IL-1β,IL-6 and TNF-αin spinal dorsal horn during inflammatory *** data indicated that endogenous NT-3 might play an anti-hyperalgesic role in inflammatory pain of rats, and the effect is possibly through the inhibition of the activation of astrocyte and microglia as well as the levels of IL-1β, IL-6,and TNF-α.

关键词： inflammatory pain NT-3 glia proinflammatory cytokines

Comprehensive Bile Acid Profiling of ABCB4-mutated Patients and the Prognostic Role of Taurine-conjugated􀀖􀄮􀀏􀀙􀄮􀀏􀀚􀄮􀀏􀀔􀀕􀄮􀀐􀀷􀁈􀁗􀁕􀁄􀁋􀁜􀁇􀁕􀁒􀁛􀁜􀁏􀁄􀁗􀁈􀁇􀀃􀀥􀁌􀁏􀁈􀀃􀀤􀁆􀁌􀁇􀀃􀁌􀁑􀀃􀀦􀁋􀁒􀁏􀁈􀁖􀁗􀁄􀁖􀁌􀁖

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Journal of Clinical and Translational Hepatology 2024年第2期12卷 151-161页

作者： Teng Liu Ren-Xue wang Jun Han Zhong-Die Li Jonathan ASheps Li-Juan Zheng Xiao-Xiao Xu Victor Ling Jian-She wang Department of Pediatrics Shanghai Medical CollegeFudan UniversityThe Center for Pediatric Liver DiseasesChildren’s Hospital of Fudan UniversityShanghaiChina BC Cancer Agency VancouverBritish Columbia V5Z 1L3Canada university of Victoria-Genome BC Proteomics Center University of VictoriaVictoriaBritish Columbia V8Z 7X8Canada shanghai Key Laboratory of Birth Defect ShanghaiChina

Background and Aims:We asked if comprehensive bile acid profiling could provide insights into the physiopathology of ABCB4-mutated patients and evaluated the prognostic value of taurine-conjugated tetrahydroxylated bile acid(tauro-THBA)in ***:Serum bile acid profiles were evaluated in 13 ABCB4-mutated patients with 65 healthy controls by ultra-high-performance liquid chromatography/multiple-reaction monitoring-mass spectrometry(UPLC/MRM-MS).The concentration of tauro-THBA was compared between ABCB4-mutated patients with different *** areas under the curve(AUCs)of tauro-THBA were compared between ABCB11-mutated patients with native liver survival and those who died or underwent liver transplantation before 3 years of age by receiver operating characteristic curve(ROC),with another patient cohort for further ***:The overall hydrophobicity indices of bile acids in ABCB4-mutated patients(12.99±3.25 m)were significantly lower than those of healthy controls(14.02.1.74 m,p11-mutated patients with different prognoses(p=0.0192).Of the 15 patients with good prognosis,14 were classified correctly and four of the five patients with a poor prognosis were classified correctly(14:15 vs.1:5,p=0.005)with tauro-THBA as a ***:Tauro-THBA concentration may be a biomarker for predicting the clinical outcome in low gammaglutamyl transferase intrahepatic cholestasis patients.

关键词： Bile acids MDR3 BSEP PFIC Liquid chromatography-mass spectrometry

Repeated clomipramine treatment reversed the cell proliferation of adult hippocampus in chronic unpredictable stress-induced depression model rats

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Repeated clomipramine treatment reversed the cell proliferat...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Qiong LIU~(1,2),Jin YU~(1,2),Qi-Liang MAO-YING~(1,2),Wen-Li MI~(1,2),yan-Qing wang~(1,2),Jun wang~(1,2),Gen-Cheng WU~(1,2,3*) 1 Department of Integrative Medicine and Neurobiology.shanghai Medical college 2 Institute of Acupuncture Research, Institutes of Brain Science 3 State Key Laboratory of Medical Neurobiology fudan university.shanghai 200032.china

＜正＞Adult hippocampal neurogenesis has been demonstrated in several species and is regulated by both environmental and pharmacological *** exposure to stress is known to induce the reduction of neurogenesis in the dentate *** present study aimed at determining whether the clinically effective antidepressant clomipramine may influence hippocampal proliferation and neurogenesis in adult rats subjected to the chronic unpredictable stress（CUS） procedure,a model of depression with predictive *** administration of clomipramine（5 mg/kg i.p.） for 3 weeks,starting 2 weeks after the beginning of the stress procedure,significantly reversed the reduction of behavior measured by open field test and forced swimming ***,rats subjected to stress exhibited a 49.9%reduction of cell proliferation at the end of a 5- week stress period,an effect which was overcome by clomipramine *** results demonstrated that exposure to CUS,which results in a state of behavioral depression,decreases hippocampal cell proliferation and that these effects can be counteracted by chronic clomipramine treatment.

关键词： clomipramine depression hippocampus neurogenesis stress rats

Efficacy and safety of anlotinib plus penpulimab as second-line treatment for small cell lung cancer:A multicenter,open-label,single-arm phase II trial

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Cancer Pathogenesis and Therapy 2024年第4期2卷 268-275页

作者： Changgong Zhang Jianhua Chen Huijuan Wu Jun wang Liying Gao Jun Zhao yan Sun Zhongyao Jia Xinlin Mu Chunmei Bai Rui wang Kailiang Wu Qiang Liu Yuankai Shi Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing Key Laboratory of Clinical Study on Anticancer Molecular Targeted DrugsBeijing 100021China Department I of Thoracic Oncology Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityChangshaHunan 410083China Department of Oncology The Affiliated Cancer Hospital of Zhengzhou UniversityZhengzhouHenan 450000China Department II of Head and Neck Tumor Gansu Provincial Cancer HospitalLanzhouGansu 730050China Department III of Radiotherapy Gansu Provincial Cancer HospitalLanzhouGansu 730050China Department I of Thoracic Oncology Beijing Cancer HospitalBeijing 100142China Department of Oncology Linyi People's HospitalLinyiShandong 276002China Department of Respiratory and Critical Care Medicine Peking University People's HospitalBeijing 100032China Department of Medical Oncology Peking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Department of Thoracic Surgery The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebei 050000China Department of Radiation Oncology Shanghai Proton and Heavy Ion CenterFudan University Cancer HospitalShanghai 200030China Department I of Oncology Shenyang Chest HospitalShenyangLiaoning 110044China

Background:Currently,the need for new therapeutic strategies involving programmed cell death protein-1(PD-1)monoclonal antibodies in the second-line setting of small cell lung cancer(SCLC)is *** study aimed to evaluate the efficacy and safety of anlotinib plus penpulimab as a second-line treatment for patients with SCLC who progressed after first-line platinum-based ***:This study included the patients from Cohort 4 of a single-arm,open-label,multicenter,phase II clinical trial.A safety run-in phase was performed under anlotinib(10/12 mg quaque die[QD],days 1–14)plus penpulimab(200 mg intravenously[IV],day 1)in a 21-day cycle,followed by the formal trial in which the patients received anlotinib(12 mg QD,days 1–14)plus penpulimab(200 mg IV,day 1)in a 21-day *** primary endpoint of the safety run-in phase was *** primary endpoint of the formal trial phase was the objective response rate(ORR).Results:From April 28,2020,to November 24,2020,21 patients were enrolled from 11 hospitals,including 2 in the safety run-in phase and 19 in the formal trial *** the formal trial phase,the ORR was 42.1.(8/19;95%confidence interval[CI]:17.7–66.6%).The median progression-free survival was 4.8 months(95%CI:2.9–11.3 months),and the median overall survival was 13.0 months(95%CI:4.6–not applicable[NA]months).The incidence of grade 3 treatment-related adverse events(TRAEs)was 52.4%(11/21),and the incidence of treatment-related serious adverse events(AEs)was 28.6%(6/21).Two AE-related deaths *** most common AEs were hypertension(57.1%,12/21),hypothyroidism(42.9%,9/21),and hypertriglyceridemia(38.1%,8/21).Conclusions:In patients with SCLC who progressed after first-line platinum-based chemotherapy,the second-line anlotinib plus penpulimab treatment demonstrates promising anti-cancer activity and a manageable safety profile,which warrants further investigation.

关键词： Anlotinib Penpulimab Small cell lung cancer Efficacy Safety Phase II trial

人纤溶酶原K5区基因真核表达载体的构建和活性鉴定

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复旦学报（医学版） 2003年第5期30卷 409-413,F002页

作者：杨健王跃祥官孝群马春姑陶贤梅宋后燕复旦大学上海医学院细胞与遗传学系-教育部分子医学重点实验室上海200032

目的　构建含人纤溶酶原K5区基因的真核表达载体 ,转染人乳腺癌细胞株MDA MB 2 31,观察阳性克隆表达的K5蛋白对人脐静脉内皮细胞株ECV30 4和MDA MB 2 31细胞增殖的影响。方法　应用PCR将人纤溶酶原信号肽序列引入K5cDNA ,所得的目的片... 详细信息

目的　构建含人纤溶酶原K5区基因的真核表达载体 ,转染人乳腺癌细胞株MDA MB 2 31,观察阳性克隆表达的K5蛋白对人脐静脉内皮细胞株ECV30 4和MDA MB 2 31细胞增殖的影响。方法　应用PCR将人纤溶酶原信号肽序列引入K5cDNA ,所得的目的片段与真核表达载体 pcDNA3重组 ,构建重组质粒pcDNA3K5 ,脂质体法将其转染MDA MB 2 31,G4 18筛选阳性克隆 ,PCR鉴定 ,RT PCR和Westernblot检测K5的表达。将鉴定正确的阳性克隆的培养上清作用于ECV30 4细胞 ,MTT法检测其增殖情况 ,并用MTT法检测转染 pcD NA3K5对MDA MB 2 31细胞增殖的影响。结果　构建的重组质粒 pcDNA3K5经酶切鉴定、测序正确 ,将其转染MDA MB 2 31后挑取的阳性克隆有 3个经PCR鉴定正确 ,并经RT PCR和Westernblot检测证实K5的表达。阳性克隆的培养上清作用于ECV30 4细胞后 ,其存活率降低 ;转染 pcDNA3K5对MDA MB 2 31细胞增殖无明显影响。结论　应用脂质体法将带有人纤溶酶原信号肽序列的K5cDNA转染MDA MB 2 31细胞后 ,其分泌产生的有生物学活性的K5 ,呈现对ECV30 4细胞增殖的抑制作用 ,而对MDA MB 2 31细胞的生长则无影响。

关键词：乳腺癌基因治疗重组新生血管生成抑制因子K5 基因表达真核表达载体构建活性鉴定

Behavior changes after inescapable shocks

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Behavior changes after inescapable shocks

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Bing LI,Qiong LIU,Xiao-Wei wang,yan-Qing wang,Jin YU,Gen-Cheng WU~* Department of Integrative Medicine and Neurobiology,Institute of Acupuncture Research,State Key Laboratory of Medical Neurobiology,Institutes of Brain Science,shanghai Medical college,fudan university,shanghai,china

＜正＞Aim Inescapable shocks（IS） have been reported to cause various post-traumatic stress disorder（PTSD）-like behavioral changes in *** aim of the present study was therefore to examine whether IS can cause depressive and anxious behaviors in this *** Wister rats were tested the behaviors before inescapable foot-shocks（IS） or not shocked（non-S） in the Elevated Pluz Maze test on day ***,rats were given either IS or non-S treatment in a box on day 1 and day *** day 5,rats treated with IS and non-S were given an avoidance/escape test in the shuttle box and elevated pluz maze test *** In accordance with previously reported results,failures and escape latencies were taken as indicators for helplessness in avoidence/escape *** the IS-treated group,the number of failures and the latency to escape were increased at a significant level in the helplessed *** about 75%IS-treated rats were nonhelplessed. The number of failures and the latency to escape of the non-helplessed rats were not significantly different with non-IS ***,the helplessed rats spent less time in the open arms and increased time in the enclosed arms compared with non-helplesssed rats and non-IS *** The behavioral changes in helplessed rats after IS treatment is similar as the patients with *** learned helplessness will be helpful for performing the further research about the mechanism of PTSD.

关键词：

混合沉积有机分子区域选择性生长的动力学蒙特卡罗模拟研究

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物理学报 2024年第12期73卷 330-337页

作者：汪长超聂青苗石亮陈乃波胡来归鄢波浙江工业大学理学院杭州310023 复旦大学信息科学与工程学院上海200433

由区域选择性生长方法(即分子在预图案表面的自组装技术)制备的有机微纳米图案有望在光电和全彩显示方面发挥巨大作用.而制备多色图案薄膜的有机器件依赖于两种及多种有机分子的成核分配,特别是混合沉积方法的应用有效提高了多色薄膜成... 详细信息

由区域选择性生长方法(即分子在预图案表面的自组装技术)制备的有机微纳米图案有望在光电和全彩显示方面发挥巨大作用.而制备多色图案薄膜的有机器件依赖于两种及多种有机分子的成核分配,特别是混合沉积方法的应用有效提高了多色薄膜成核的效率.本文利用动力学蒙特卡罗方法研究了两种分子间的结合能强弱对混合沉积两种分子以实现相分离式生长的影响.结果表明:当分子间结合能较弱时,两种分子表现出完全不同的相分离生长行为,绝大部分的非平面和平面分子分别沉积在电极顶部和侧面;当分子间结合能较强时,电极顶部及侧面都出现了两类分子的掺杂生长,导致了分子相分离生长的失效.研究证明了分子间的结合能会影响多种有机分子选区生长分离的效果,因此混合沉积两种有机分子时,通过适当调整分子间的结合能,在电极顶部和侧面可分别获得较纯结晶的单色薄膜,从而促进了多色有机微纳米图案在微显示等领域的进一步应用.

关键词：区域选择性生长动力学蒙特卡罗有机分子相分离

Robust spinal neuroinflammation mediates bilateral mechanical allodynia induced by intra-tibia inoculation of Walker 256 cancer cells in rats

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Robust spinal neuroinflammation mediates bilateral mechanica...

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Qi-Liang MAO-YING,Xiao-Wei wang,Xiu LI,Wen-Li MI,Gen-Cheng WU,yan-Qing wang Department of Integrative Medicine and Neurobiology,State Key Laboratory of Medical Neurobiology,Institutes of Brain Science, shanghai Medical college,fudan university,shanghai 200032,china

＜正＞It has been reported that remarkable and sustained activation of spinal astrocyte and/or microglia induced by bone cancer pain in the animals,which were quite different from the neuropathic and inflammatory pain. The present study was designed to investigate the role of spinal Toll-like receptor 4（TLR4）-induced glial neuroinflammation,including the astrocyte and microglia activation,proinflammatory expression,in bone cancer pain in a modified rat *** results showed that,eight days after unilateral intra-tibial inoculation of syngeneic Walker 256 mammary gland carcinoma cells,a robust activation of both astrocyte and microglia in bilateral spinal dorsal horn,characterized by enhanced immunostaining of both glial fibrillary acidic protein（GFAP,astrocyte marker） and OX-42（microglia marker）,and elevated level of IL-1β,IL-6 and TNF-αmRNA,was observed as well as significant bilateral mechanical *** mechinacal allodynia and spinal neuroinflammation were sustained until 22 days after intra-tibial *** investigate the role of spinal astrocyte and microglia in the bone cancer pain,single dose of fluorocitrate（inhibitor of glial metabolism,1 nmol） or minocycline（inhibitor of microglia,100μg） were intra-thecal administered by lumbar puncture on day 12 after Walker 256 *** results showed that spinal administration of fluorocitrate or minocycline has significant anti-allodynic effects in this bone cancer pain rats,suggesting that spinal astrocyte and microglia were involved in the process of bone cancer *** has been reported that TLR4 is almost exclusively expressed by microglia and a very intriguing mechanism proposed for spinal microglial activation in response to peripheral nerve injury is activation of *** order to confirm whether TLR4 was involved in bone cancer pain,nonstereoselective TLR4 receptor antagonist naloxone（60μg,i.t.） was also intra-thecally *** result showed that it could significantly block the incre

关键词： bone cancer pain mechanical allodynia neuroinflammation Toll-like receptor 4 rat

逆向云灰色关联相似日的EEMD-RL-GWO-LSTM区域风光功率短期预测

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太阳能学报 2024年第10期45卷 144-152页

作者：张宇华时鑫洋颜楠楠王育飞薛花上海电力大学电气工程学院复旦大学类脑智能科学与技术研究院国网华东电力试验研究院

针对现有方法在风光预测时气象因素考虑不全面且未考虑风光功率关联性的问题，提出一种风光功率短期预测方法。首先，以云模型表征风光出力不确定性，逆向云结合灰色关联度分析不同气象特征对输出功率的影响程度，并设立选取标准及综合... 详细信息

针对现有方法在风光预测时气象因素考虑不全面且未考虑风光功率关联性的问题，提出一种风光功率短期预测方法。首先，以云模型表征风光出力不确定性，逆向云结合灰色关联度分析不同气象特征对输出功率的影响程度，并设立选取标准及综合评分指标；其次，采用集合经验模态分解（EEMD）将选取相似日的功率数据分解为子序列；最后，将子序列和气象数据作为基于折射学习策略（RL）的灰狼算法（GWO）优化的改进长短期记忆网络（LSTM）模型的预测输入进行训练，对待预测日的子序列分别预测，并叠加得到短期区域风光发电功率的预测。以中国西北某风光联合电场数据为例，对该模型进行验证，结果表明，相比于现有预测模型，该文所提方法考虑了天气因素，具有较高的预测精度，能够较好地为区域风光联合电场的功率预测提供参考。

关键词：逆向云灰色关联相似日集合经验模态分解 RL-GWO-LSTM神经网络短期风光功率预测