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间充质干细胞及其衍生细胞外囊泡靶向巨噬细胞干预自身免疫性疾病

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中国组织工程研究 2025年第31期29卷 6772-6781页

作者：姚兰宣王雪菲刘洋杨雨佳赵怡齐芳芳李颖辉河北医科大学口腔医学院河北省石家庄市050000 河北省口腔医学重点实验室河北省石家庄市050000 河北医科大学口腔医院牙体牙髓科河北省石家庄市050000 河北医科大学口腔医院正畸科河北省石家庄市050000

背景:巨噬细胞是机体固有免疫的重要组成部分,当机体内环境发生改变时巨噬细胞可以产生不同的极化表型,并发挥相应的炎性免疫作用。间充质干细胞能够分泌较多数量的细胞外囊泡到机体内环境中,具有细胞间信号传导及免疫调节功能。研究表... 详细信息

背景:巨噬细胞是机体固有免疫的重要组成部分,当机体内环境发生改变时巨噬细胞可以产生不同的极化表型,并发挥相应的炎性免疫作用。间充质干细胞能够分泌较多数量的细胞外囊泡到机体内环境中,具有细胞间信号传导及免疫调节功能。研究表明,间充质干细胞及间充质干细胞来源细胞外囊泡可以影响巨噬细胞M1/M2极化平衡,从而治疗机体免疫炎症性疾病。目的:探讨间充质干细胞及其来源细胞外囊泡通过调节巨噬细胞极化来干预自身免疫性疾病的信号机制,以及工程化细胞外囊泡在此领域的相关研究进展。方法:第一作者检索PubMed、中国知网等数据库自建库到2024年6月发表的相关文献,以“间充质干细胞,细胞外囊泡,外泌体,凋亡小体,凋亡囊泡,巨噬细胞极化,M1极化,M2极化,自身免疫性疾病,多发性硬化,类风湿性关节炎,系统性红斑狼疮,1型糖尿病,炎症性肠病,自身免疫性泪腺炎,工程化细胞外囊泡,工程化外泌体,药物递送”为中文检索词;以“macrophage polarization,M1 macrophage,M2 macrophage,autoimmune disease,type 1 diabetes,multiple sclerosis,rheumatoid arthritis,systemic lupus erythematosus,autoimmune dacryadenitis,inflammatory bowel disease,mesenchymal stem cells,extracellular vesicles,engineered extracellular vesicles,engineering exosomes,drug delivery”为英文检索词,阅读每篇文献的文题、摘要进行初筛,最终筛选出70篇文献进行归纳分析。结果与结论:①间充质干细胞可以通过释放或间接作用于功能性蛋白来调节M1/M2极化;②间充质干细胞可以通过作用于炎症小体调控巨噬细胞M2极化;③间充质干细胞可以与常用药物组合以增强药物疗效;④间充质干细胞受到炎性刺激后可以调控细胞外囊泡的释放影响巨噬细胞极化;⑤间充质干细胞来源细胞外囊泡可以通过PTEN、NOTCH、核因子κB、Toll样受体、PI3K/AKT等通路靶向巨噬细胞极化调节自身免疫性疾病;⑥工程化细胞外囊泡可以实现非侵入式靶向给药,延长药物半衰期,推动外泌体口服给药,减轻移植物异体反应,提高中草药生物利用度并攻克血脑屏障,开辟了一条药物递送新路径。

关键词：间充质干细胞细胞外囊泡外泌体巨噬细胞极化 M1/M2极化自身免疫性疾病工程化细胞外囊泡

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Interactions between meso-tetrakis(4-(N-methylpyridiumyl))porphyrin TMPyP4 and DNA G-quadruplex of telomeric repeated sequence TTAGGG

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Science China Chemistry 2008年第5期51卷 452-456页

作者： ZHANG HuiJuan wang xuefei wang Peng PANG SiPing AI XiCheng ZHANG JianPing School of Life Science&Technology Beijing Institute of TechnologyBeijing 100081China Department of Chemistry Renmin University of ChinaBeijing 100872China Institute of Chemistry Chinese Academy of SciencesBeijing 100080China

The binding properties between meso-tetrakis(4-(N-methylpyridiumyl))porphyrin (TMPyP4) and the parallel DNA G-quadruplex (G4) of telomeric repeated sequence 5′-TTAGGG-3′ have been characterized by means of circular dichroism,steady-state absorption,steady-state fluorescence and picosecond time-resolved fluorescence spectroscopies. The binding constant and the saturated binding number were determined as 1.29×106 (mol/L)-1 and 3,respectively,according to steady-state absorption spec-troscopy. Based on the findings by the use of time-resolved fluorescence spectroscopic technique,it is deduced that TMPyP4 binds to a DNA G-quadruplex with both the thread-intercalating and end-stacking modes and at the saturated binding state,one TMPyP4 molecule intercalates into the intervals of G-tetrads while the other two stack to the ends of the DNA G-quadruplex.

关键词： TMPyP4 DNA G-quadruplex spectroscopic technique binding mode

Ba_(2)B_(9)O_(13)F_(4)·BF_(4):first fluorooxoborate with unprecedented infinite [B_(18)O_(26)F_(8)] tubes and deep-ultraviolet cutoff edge

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Science China Chemistry 2023年第4期66卷 1073-1077页

作者： Huan Pei xuefei wang Jie Zhang Fangfang Zhang Zhihua Yang Shilie Pan Research Center for Crystal Materials CAS Key Laboratory of Functional Materials and Devices for Special EnvironmentsXinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqi 830011China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Fluorooxoborate has emerged as a promising system for exploring optical materials due to their rich structural chemistry and physicochemical properties,yet so far not fully ***,we report a new fluorooxoborate,Ba_(2)B_(9)O_(13)F_(4)·BF_(4),which contains the greatest variety of structural basic units,[BO_(3)],[BO_(2)F_(2)],[BO_(3)F],and[BF_(4)],and exhibits unprecedented 1D infinite[B_(18)O_(26)F_(8)]*** unique structure avoids the dangling bonds caused by terminal oxygens,which is beneficial to the large bandgap and deep-ultraviolet cutoff *** article expands the crystal structure of fluorooxoborates and contributes to the exploration of deep-ultraviolet optical materials in fluorooxoborate systems.

关键词： fluorooxoborate unprecedented infinite tubes four basic units deep-ultraviolet

Effects of laser powder bed fusion process parameters on microstructure and hydrogen embrittlement of high-entropy alloy

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Journal of Materials Science & Technology 2023年第24期155卷 211-226页

作者： Hongxu Cheng Hong Luo Zhimin Pan xuefei wang Qiancheng Zhao Yu Fu Xiaogang Li National Materials Corrosion and Protection Data Center Institute for Advanced Materials and Technology University of Science and Technology Beijing Beijing 100083 China Key Laboratory for Corrosion and Protection of The Ministry of Education (MOE) Beijing 100083 China

Hydrogen embrittlement behavior, micro-deformation, and crack propagation mechanism of CoCrFeNiMn high-entropy alloy (HEA) fabricated by laser powder bed fusion (LPBF) under different parameters were investigated by slow strain rate tensile tests (at room temperature) with/without electrochemical hydrogen pre-charging. The LPBF CoCrFeNiMn HEA shows excellent resistance to hydrogen embrittlement. Unsuitable LPBF parameters are accompanied by many microcracks and holes, resulting in a slight decrease in the hydrogen embrittlement resistance of the material. The electron backscatter diffraction (EBSD), electron channeling contrast image (ECCI) techniques, and transmission electron microscope (TEM) were carried out to research the main influencing factors of hydrogen on the deformation mechanism and crack propagation. Compared with un-charged samples, a larger number of deformation twins (DTs) appear in the deformation process of hydrogen-charged LPBF CoCrFeNiMn, attributing to the reduction of stacking fault energy (SFE) due to the ingress of hydrogen. The nano DTs and crossing twin system contribute to the extra work hardening, and a strain hardening platform is observed for all hydrogen-charged samples, resulting in the increase of strain hardening rate or the mitigation of the loss of strain hardening. Although unsuitable process parameters will trigger fabrication defects and reduce mechanical properties, the cellular structure can bring a hydrogen-induced strain hardening platform for LPBF CoCrFeNiMn to reduce the damage caused by hydrogen embrittlement.

关键词： Laser powder bed fusion High-entropy alloy Hydrogen embrittlement Deformation mechanism

Micro-destructive assessment of subgrade compaction quality using ultrasonic pulse velocity

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第11期16卷 4782-4797页

作者： xuefei wang Xuping Dong Xiangdong Li Jianmin Zhang Guowei Ma Jiale Li School of Civil and Transportation Engineering Hebei University of TechnologyTianjinChina Department of Hydraulic Engineering State Key Laboratory of Hydroscience and EngineeringTsinghua UniversityBeijingChina

The ultrasonic pulse velocity(UPV)correlates significantly with the density and pore size of subgrade filling *** research conducts numerous Proctor and UPV tests to examine how moisture and rock content affect compaction *** study measures the changes in UPV across dry density and compaction *** compacted specimens exhibit distinct microstructures and mechanical properties along the dry and wet sides of the compaction curve,primarily influenced by internal water *** maximum dry density exhibits a positive correlation with the rock content,while the optimal moisture content demonstrates an inverse *** the rock content increases,the relative error of UPV measurement *** UPV follows a hump-shaped pattern with the initial moisture *** intelligent models are established to forecast dry *** measure of UPV and PSO-BP-NN model quickly assesses compaction quality.

关键词： Subgrade Compaction quality Ultrasonic pulse velocity(UPV) Intelligent model Proctor test Micro-destructive evaluation

Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission

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Frontiers of Medicine 2017年第4期11卷 548-553页

作者： Xiaohong Shi xuefei wang Xixi Xu Yongliang Feng Shuzhen Li Shuying Feng Bo wang Suping wang Department of Epidemiology School of Public Health Shanxi Medical University Taiyuan 030001 China Department of Obstetrics and Gynaecology The Third PeopIeHospital of Taiyuan City Taiyuan 030001 China

This study determined the effect of hepatitis B virus （HBV） replication in peripheral blood mononuclear cell （PBMC） from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg- positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA （rcDNA）, and covalently closed circular DNA （cccDNA） were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi- square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% （12/150） in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cceDNA in the HBsAg- positive mothers were 36.67% （55/150） and 10% （15/150）, respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission （OR = 6.003, 95% Ch 1.249-28.855）. Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA （OR = 3.896, 95% CI： 1.929-7.876） and PBMC HBV cccDNA （OR = 3.74, 95% CI： 1.186--11.793） in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor ofPBMC HBV cccDNA （OR = 0.312, 95% CI： 0.102-0.954） during pregnancy. The positivity ofPBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers （Zz= 5.087, P = 0.024）. This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.

关键词： PBMC HBV cccDNA HBV rcDNA HBV intrauterine transmission

Effects of acupuncture on functional gastrointestinal disorders:special effects,coeffects,synergistic effects in terms of single or compatible acupoints

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Journal of Traditional Chinese Medicine 2023年第2期43卷 397-408页

作者： ZHAO Jun LI Xia ZHENG Hui YE Kun wang Xin wang xuefei SUN Runquan LI Zhigang School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing 100029China Chengdu University of Traditional Chinese Medicine Chengdu 610075China Department of Acupuncture and Moxibustion The Sixth Medical Center of PLA General HospitalBeijing 100048China Department of Acupuncture and Moxibustion Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China

OBJECTIVE:To investigate how the“special effect”/“coeffect”/“synergistic effect”can achieve a positive result using an“acupuncture prescription”for functional gastrointestinal disorders(FGIDs)in clinical practice,based on the characteristics of single/compatible acupoints and acupuncture ***:According to the search strategy,we searched six electronic bibliographic databases and provided a summary for this ***:A large body of evidence has shown that acupuncture has positive effects in the treatment of ***,the“prescription”intervention involved different single acupoints,compatible acupoints and acupoints based on expert ***:The core acupoints,including Tianshu(ST25),Zusanli(ST36),and Shangjuxu(ST37),emphasize the application of special acupoints,meridian points and nerve segments,and the two-way regulatory effect found in this study is often used as the basis of acupoint selection and acupoint prescription for acupuncture treatment of FGIDs.

关键词： acupuncture gastrointestinal diseases acupuncture points special effects co-effects synergistic effects

Mechanism of intram olecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine

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Science China Chemistry 2004年第5期47卷 367-372页

作者： ZHANG Huijuan wang Peng wang xuefei FENG Juan XU Sichuan AI Xicheng ZHANG Xingkang ZHANG Jianping State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

As a structural analogue of adenine, 2-aminopurine (2Ap) is often used as a fluorescent probe to study the intramolecular charge transfer reaction in DNA. We have designed and synthesized a series of model DNA helix with the variation in the distance between the 2Ap probe and the GGG sequence, and have investigated, by means of picosecond time-resolved fluorescence spectroscopy, the effect of the length of the bridge (consisting of a number of inosines, I) separating the electron donor (???GGG???) and the acceptor (2Ap) on the charge transfer dynamics. The fluorescence dynamics of 2Ap exhibited three exponential decay components, the one with a time constant of a few hundred picoseconds is assigned to the intramolecular charge transfer from GGG to 2Ap. Within 2.4 nm of the donor-acceptor separation, the rate of charge transfer decreased exponentially upon increasing the separation, from which the decay factor β is determined to be 1.3 nrrf-1. Beyond 2.4 nm, however, the rate started toincrease, this abnormal behavior of charge transfer is interpreted in terms of the match of elec- tronic energies between the l-bridge and the donor/acceptor couple.

关键词： 2-aminopurine(2Ap) DNA charge transfer time-resolved fluorescence spectroscopy.

Evaluation and impact factors of indoor and outdoor gas-phase nitrous acid under different environmental conditions

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Journal of Environmental Sciences 2020年第9期32卷 165-171页

作者： Yan Chen Weigang wang Chaofan Lian Chao Peng Wenyu Zhang Junling Li Mingyuan Liu Bo Shi xuefei wang Maofa Ge State Key Laboratory for Structural Chemistry of Unstable and Stable Species Beijing National Laboratory for Molecular Sciences(BNLMS)CAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China School of Chemical Sciences University of Chinese Academy of SciencesBeijing 100049China Center for Excellence in Regional Atmospheric Environment Institute of Urban EnvironmentChinese Academy of ScienceXiamen 361021China

As an important indoor pollutant,nitrous acid(HONO) can contribute to the concentration of indoor OH radicals by photolysis via sunlight penetrating into indoor environments,thus affecting the indoor oxidizing *** order to investigate the concentration of indoor HONO and its impact factors,three different indoor environments and two different locations in urban and suburban areas were selected to monitor indoor and outdoor pollutants simultaneously,including HONO,NO,NO2,nitrogen oxides(NOx),O3,and particle mass *** general,the concentration of indoor HONO was higher than that *** the urban area,indoor HONO with high average concentration(7.10 ppbV) was well-correlated with the *** the suburban area,the concentration of indoor HONO was only about 1-2 ppbV,and had a good correlation with indoor relative *** was mainly attributed to the heterogeneous reaction of NO2 on indoor *** sunlight penetrating into indoor environments from outside had a great influence on the concentration of indoor HONO,leading to a concentration of indoor HONO close to that outdoors.

关键词： impact environments outdoor