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Withdrawal Weighting Image-Impedance Connected SAW Filters with Al-Mo Alloy Electrodes

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稀有金属材料与工程 2006年第A03期35卷 623-624页

作者： Dongmei Li xubo wang Feng Pan Laboratory of Advanced Materials Department of Materials Science and Engineering Tsinghua University Beijing 100084 China College of Chemistry and Chemical Engineering Xinjiang University Wulumuqi 830046 China

To obtain high reliability of surface acoustic wave(SAW)devices,Mo was added to Al electrodes on 128°Y-X LiNbO_3 substrates by a *** sputtering *** of Mo additive on the mechanical properties of Al films was *** was found that the Al-Mo alloy films displayed a superior adhesion to *** the Al-Mo alloy films,a 1.65 GHz-range image-impedance connected SAW filter was designed and *** this structure of SAW filter, for satisfying stopband requirements a dual-track structure was *** weighting of interdigital transducers(IDT) was used to adjust the bandwidth of SAW filter.

关键词： Al-Mo films adhesion surface acoustic wave

Development of polymorphic SSR markers and their applicability in genetic diversity evaluation in Euptelea pleiosperma

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BIOCELL 2022年第11期46卷 2489-2495页

作者： XIAOJUN ZHOU XIAOYU LU xubo wang College of Life Science Luoyang Normal UniversityLuoyang471934China

Euptelea pleiosperma is a characteristic species of East Asian flora with both ornamental and scientific *** on the reduced-representation sequencing(RRS)technology of RAD-Seq,this study conducted high-throughput Illumina paired-end sequencing to find SSR marker information in the genome of ***,and to screen and verify polymorphism of SSR *** obtained 5.5G of high-quality data using *** total number of contigs of the RAD tags was 299,376,with the maximum contig length of 2,062 bp and the average length of 445 *** these sequences,we identified 20,718 SSR loci,with a distribution density of one SSR per 6.45 kb(1/6.45 kb).Among all SSRs,dinucleotides(52.00%)were the most detected SSRs,followed by mononucleotides(21.63%).AG/CT was the dominant motif in the SSR loci,accounting for 34.8%.Primers were successfully designed for 14,593 loci,and 100 pairs of these primers were randomly selected for chemical synthesis and validated by SSR-PCR amplification in 20 individuals of ***-nine primers were able to amplify the target *** 3.0 software was used to analyze the selected 20 SSR loci with good *** the 20 SSR markers,the number of alleles ranged from 4 to 9,and the observed heterozygosity and expected heterozygosity were from 0.35 to 0.75 and 0.541 to 0.875,*** information content of polymorphic loci ranged from 0.463 to 0.848,with an average value of *** them,there were 18 highly polymorphic loci,and 20 SSR loci did not deviate from the Hardy-Weinberg ***,the 20 pairs of SSR primers were used to conduct PCoA analysis based on Nei’s genetic distance of 51 individuals from three *** results showed that these SSR markers could distinguish genetic differences based on different geographical locations.

关键词： Cluster analysis E.pleiosperma RAD-Seq Rare species SSR markers

Genetic Variation Assessed with Microsatellites in Mass Selection Lines of the Pacific Oyster (Crassostrea gigas) in China

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Journal of Ocean University of China 2016年第6期15卷 1039-1045页

作者： wang xubo LI Qi YU Hong KONG Lingfeng Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China

Abstract Four successive mass selection lines of the Pacific oyster, Crassostrea gigas, selected for faster growth in breeding pro- grams in China were examined at ten polymorphic microsatellite loci to assess the level of allelic diversity and estimate the effective population size. These data were compared with those of their base population. The results showed that the genetic variation of the four generations were maintained at high levels with an average allelic richness of 18.8-20.6, and a mean expected heterozygosity of 0.902-0.921. They were not reduced compared with those of their base population. Estimated effective population sizes based on temporal variances in microsatellite frequencies were smaller to that of sex ratio-corrected broodstock count estimates. Using a rela- tively large number ofbroodstock and keeping an equal sex ratio in the broodstock each generation may have contributed to retaining the original genetic diversity and maintaining relatively large effective population size. The results obtained in this study showed that the genetic variation was not affected greatly by mass selection progress and high genetic variation still existed in the mass selection lines, suggesting that there is still potential for increasing the gains in future generations of C. gigas. The present study provided im- portant information for future genetic improvement by selective breeding, and for the design of suitable management guidelines for genetic breeding of C. gigas.

关键词： Pacific oyster genetic variation microsatellite mass selection line effective population size

The Fabrication of 2.5 GHz SAW Filter Using ZnO/Diamond Structure

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稀有金属材料与工程 2006年第A03期35卷 538-539页

作者： xubo wang Jingjing Chen Dongmei Li Fei Zeng Feng Pan Laboratory of Advanced Materials Department of Materials Science and Engineering Tsinghua University Beijing 100084 China

Great interest in surface acoustic wave device based on diamond is attracted because diamond has the highest surface acoustic wave velocity of all *** this paper,400 nm ZnO film was deposited on polycrystalline diamond wafer by magnetron *** experimental results indicate that highly c-axis oriented and fine-grain polycrystalline ZnO films have been obtained.100 nm Al thin film was deposited on ZnO/diamond by electron *** interdigital-transducers with the electrodes of 1.0μm width were fabricated on the Al film using photolithography and reactive ion etching (RIE) processes.A 2.48 GHz longitudinally-coupled double-mode surface acoustic wave filter has been successfully fabricated using the Al/ZnO/diamond structure.

关键词： surface acoustic wave filter ZnO film diamond wafer

Giant piezoresponse and promising application of environmental friendly small-ion-doped ZnO

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Science China(Technological Sciences) 2012年第2期55卷 421-436页

作者： PAN Feng LUO JingTing ANG YuChao wang xubo ZENG Fei Key Laboratory of Advanced Materials Department of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

In recent years, with the growing concerns on environmental protection and human health, new materials, such as lead-free piezoelectric materials, have received increasing attention. So far, three types of lead-free piezoelectric systems have been widely researched, i.e., perovskites, bismuth layer-structured ferroelectrics, and tungsten-bronze type ferroelectrics. This article presents a new type of environmental friendly piezoelectric material with simple structure, the transition-metal（TM）-doped ZnO. Through substituting Zn2＋ site with small size ion, we obtained a series of TM-doped ZnO with giant piezoresponse, such as Zno.975Vo.o250 of 170 pC/N, Zn0.94Cr0.06O of 120 pC/N, Zn0.913Mn0.0870 of 86 pC/N and Zn0.988Fe0.0120 of 127 pC/N. The tremendous piezoresponses are ascribed to the introduction of switchable spontaneous polarization and high permittivity in TM-doped ZnO, The microscopic origin of giant piezoresponse is also discussed. Substitution of TM ion with small ionic size for Zn2＋ results in the easier rotation of noncollinear TM-O1 bonds along the c axis under the applied field, which produces large piezoelectric displacement and corresponding piezoresponse enhancement. Furthermore, it proposes a general rule to guide the design of new wurtzite semiconductors with enhanced piezoresponses. That is, TM-dopant with ionic size smaller than Zn2＋ substitutes for Zn2＋ site will increase the piezoresponse of ZnO significantly. Finally, we discuss the improved per- formances of some TM-doped ZnO based piezoelectric devices.

关键词： ZnO lead-free piezoelectric doping modification piezoresponse enhancement

Establishment and Characterization of Four Long-Term Cultures of Neural Stem/Progenitor Cell Lines from the Japanese Flounder Paralichthys olivaceus

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Journal of Ocean University of China 2020年第5期19卷 1153-1162页

作者： GAO Chen SONG Huayu wang Mengxun LIU Xiumei ZHAO Jun wang xubo ZHANG Quanqi Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Qingdao Conson Oceantec Valley Development Co. Ltd.Qingdao 266003China

Neurogenesis is an important progress wherein the neural stem cells(NSCs) differentiate into functional neurons under conductive conditions. Neurogenesis occurs continuously in different areas of the central neural system in adult teleosts compared with adult mammals. Therefore, NSC cell lines must be established to offer a valuable in vitro system for studies on neurogenesis and other related functions. In this study, four cell lines designated as PoB1, PoB2, PoBf and PoBh were established from the brain of the Japanese flounder Paralichthys olivaceus. The cell lines were sub-cultured over 150 times and still grew well in DMEM/F12 medium at 24℃. PoB1, PoB2, PoBf and PoBh were identified as neural stem/progenitor cell lines on the basis of the mRNA expression of nestin and/or aldh111 or slc6 a4 and the formation of neurospheres. The cells transfected with the pEGFP-C1 plasmid showed fluorescent signals with distinct reagent dependencies. The established cell lines from the brain of P. olivaceus offer a valuable system in vitro for the study of neurogenesis, fish neural regulation and endocrinology-related functions.

关键词： brain cell lines Paralichthys olivaceus transfection nestin

Genome-Wide Identification of heat shock protein 10/60 Genes in Japanese Flounder (Paralichthys olivaceus) and Their Regulated Expression After Bacterial Infection

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Journal of Ocean University of China 2021年第4期20卷 886-896页

作者： YAN Weijie QIAO Yingjie LIU Yuxiang LIU Xiumei ZHANG Quanqi wang xubo Key Laboratory of Marine Genetics and Breeding Ministry of EducationOcean University of ChinaQingdao 266003China College of Life Sciences Yantai UniversityYantai 264005China

Heat shock proteins 10/60(hsp10/60)are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful *** the chaperone and housekeeping functions,they are also known to be involved in immune response during bacterial *** this study,we identified and annotated 10 hsp10/60 genes through bioinformatic analysis in Japanese flounder(Paralichthys olivaceus).Among them one member of hsp10(hspe)family and nine members of hsp60(hspd)family were *** and selection pressure analysis showed that the hsp10/60 genes were evolutionarily constrained and their function was ***,hsp10/60 genes were involved in different embryonic and larval stages and acted as the sentinel role in an unchallenged *** addition,we also observed the expression patterns of hsp10/60 genes after Edwardsiella tarda infection,for the first time in Japanese *** out of 10 genes were differentially expressed after bacterial challenges,the significantly regulated expressions of flounder hsp10/60 genes after bacterial infections suggested their involvement in immune response in *** results provide valuable information for clarifying the evolutionary relationship,and early insights of the immune functions of hsp10/60 genes in Japanese flounder.

关键词： heat shock protein Hsp10/60 Japanese flounder Edwardsiella tarda immune response

Characterization of the first mitochondrial genome of Aclerdidae (Hemiptera: Coccoidea) with a novel gene arrangement

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Zoological Systematics 2022年第4期47卷 293-304页

作者： Qingsong Zhou Mei Xiong Arong Luo xubo wang San’an Wu Key Laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China College of Biological Sciences University of Chinese Academy of SciencesBeijing 100049China Southwest Forestry University Kunming 650224China The Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry UniversityBeijing 100083China

Scale insects comprise a diverse group of insects within superfamily Coccoidea in the suborder Sternorrhyncha,many of them are important pests of economic *** advent in next-generation sequencing(NGS)technologies has revolutionized conventional phylogenomic researches,causing a rapid accumulation of sequenced insect mitochondrial ***,there have been few studies on the mitochondrial genome of diverse scale insect *** using NGS methods,the complete mitochondrial genome of Nipponaclerda biwakoensis was obtained in this study,representing the first mtDNA of the family *** whole mitochondrial genome is 16,654 bp in length with an A+T content of 81.3%,including 13 protein-coding genes(PCGs),2 ribosomal RNAs,17 transfer RNAs and a control *** PCGs begin with typical ATN codons and use standard TAA as their termination codons,except nad1 gene stopping with ***,this mitochondrial genome shows a unique gene rearrangement with a long fragment(trnP-trnC-trnI-nad2-trnY-trnT)insertion in the conserved region nad6-cob,which is novel to Hemiptera,even *** phylogeny reconstruction of Sternorrhyncha based on 13 PCGs and 2 rRNAs under Bayesian Inference method support a robust topology that Aleyrodoidea is the earliest branch of Sternorrhyncha,with Psylloidea as the sister group of the(Aphidoidea+Coccoidea)clade.

关键词： Mitochondrial genome scale insects gene arrangement phylogeny Nipponaclerda biwakoensis

Tumor necrosis factor-alpha(TNF-α) in spotted halibut Verasper variegatus at the embryonic and metamorphic stages

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Journal of Oceanology and Limnology 2020年第2期38卷 454-466页

作者： LI Zan LIU Xiumei DU Xinxin ZHANG Kai CHEN Yan wang xubo wang Zhigang YU Haiyang ZHANG Quanqi School of Agriculture Ludong UniversityYantai 264025China College of Life Sciences Yantai UniversityYantai 264005China Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life ScienceOcean University of ChinaQingdao 266003China

As an aquatic fish,the spotted halibut Verasper variegatus is highly susceptible to bacterial and virus *** necrosis factor-alpha(TNF-α)as a cytokine could control the inflammatory *** functions of TNF-αin many species have been widely studied,particularly in ***,little is known about the TNF-αfunctions in *** first cloned and sequenced the TNF-αgene in ***(VvTNF-α).The two conserved cysteine residues,transmembrane sequence,Thr-Leu motif,and TNF family signature,as well as the TA-rich motifs of its proteins related to inflammatory responses had high similarity to those of the other teleost and mammalian TNF-α.The phylogenetic analysis showed that VvTNF-αwas consistent with TNF-αgenes of other *** VvTNF-αtranscripts were extensively distributed in the peripheral blood leukocytes(PBLs),spleen,and gill,indicating that the VvTNF-αhad a role in immune ***,treatment with pathogen-associated molecular patterns(PAMPs)could induce a rapid and significant increase of VvTNF-αin the PBLs,which reveals that VvTNF-αdoes participate in the host immune responses against bacterial and viral *** found that VvTNF-αhad an interesting expression pattern during metamorphosis,showing that the flatfish TNF-αmay have some novel functions during specific developmental *** addition,the 3 D structure prediction of VvTNF-αprovided an indication of how it is likely to interact with other ***,VvTNF-αhas multiple functions,and provides valuable information to explore novel functions of TNF-α.

关键词： tumor necrosis factor-alpha(TNF-α) Verasper variegatus metamorphosis peripheral blood leukocytes(PBLs) 3D modeling pathogen-associated molecular patterns(PAMPs)