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Research 2022年第3期2022卷 205-223页

作者： Kunming Zhao xingwen wang Dong Zhao Qingyu Lin Yi Zhang Ying Hu School of Life Science and Technology Harbin Institute of TechnologyHarbinHeilongjiang ProvinceChina 150001 School of Public Health Qingdao UniversityQingdaoChina 266071

Lactic acid acidifies the tumor microenvironment and promotes multiple critical oncogenic processes,including immune *** kinase M2(PKM2)is a dominant form of pyruvate kinase(PK)expressed in cancers that plays essential roles in metabolic reprograming and lactate production,rendering it as an attractive therapeutic target of ***,the mechanism underlying PKM2 regulation remains ***,we show that long noncoding RNA(lncRNA)HIF-1αinhibitor at transcription level(HITT)inhibits lactate production in a PKM2-dependent ***,it physically interacts with PKM2 mapped to a region that has been involved in both dimer(less-active)and tetramer(more-active)formation,inhibiting PKM2 oligomerization and leading to dramatic reduction of PK *** glucose starvation,HITT was reduced as a result of miR-106 induction,which subsequently facilitates PKM2 oligomerization and increases vulnerability to apoptosis under glucose starvation *** addition,the interaction also reduces lactate secretion from cancer cells,which subsequently polarizes macrophages toward an M2-like anti-inflammatory phenotype and thus possibly contributes to immune escape in *** study highlights an important role of an lncRNA in regulating PKM2 activity and also reveals a metabolic regulatory effect of PKM2 on macrophage polarization.

关键词： PKM2 Polarization rendering

Pseudo-beam method for compressive buckling characteristics analysis of space inflatable load-carrying structures

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Acta Mechanica Sinica 2009年第5期25卷 659-668页

作者： Changguo wang Huifeng Tan xingwen Du Center for Composite Materials,Harbin Institute of Technology, 150001 Harbin, China Center for Composite Materials Harbin Institute of Technology 150001 Harbin China

This paper extends Le van＇s work to the case of nonlinear problem and the complicated configuration. The wrinkling stress distribution and the pressure effects are also included in our analysis. Pseudo-beam method is presented based on the inflatable beam theory to model the inflatable structures as a set of inflatable beam elements with a prestressed state. In this method, the discretized nonlinear equations are given based upon the virtual work principle with a 3-node Timoshenko＇s beam model. Finite element simulation is performed by using a 3-node BEAM189 element incorporating ANSYS nonlinear program. The pressure effect is equivalent included in our method by modifying beam element cross-section parameters related to pressure. A benchmark example, the bending case of an inflatable cantilever beam, is performed to verify the accuracy of our proposed method. The comparisons reveal that the numerical results obtained with our method are close to open published analytical and membrane finite element results. The method is then used to evaluate the whole buckling and the loadcarrying characteristics of an inflatable support frame subjected to a compression force. The wrinkling stress and region characteristics are also shown in the end. This method gives better convergence characteristics, and requires much less computation time. It is very effective to deal with the whole load-carrying ability analytical problems for large scale inflatable structures with complex configuration.

关键词： Inflatable structure - Pseudo-beam method Inflatable beam theory Nonlinear buckling finite element

Introduction of antisense waxy gene into main parent lines of indica hybrid rice

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Chinese Science Bulletin 2002年第14期47卷 1192-1197页

作者： CHEN Xiuhua LIU Qiaoquan wang Zongyang wang xingwen CAI Xiuling ZHANG Jingliu GU Minghong Key Laboratory of Biotechnology of Jiangsu Province Yangzhou UniversityYangzhou 225009China Shanghai Institute of Plant Physiology and Ecology Chinese Academy of ScienceShanghai 200032China

Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains

关键词： transgenic rice indica rice maintain line antisense waxy gene amylose content.

Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua

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Horticulture Research 2023年第9期10卷 220-232页

作者： xingwen wang Lan Wu Li Xiang Ranran Gao Qinggang Yin Mengyue wang Zhaoyu Liu Liang Leng Yanyan Su Huihua Wan Tingyu Ma Shilin Chen Yuhua Shi Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine Artemisinin Research CenterInstitute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Institute of Herbgenomics Chengdu University of Traditional Chinese MedicineChengdu 611137China Amway(China)Botanical R&D Center Wuxi 214115China Key Lab of Chinese Medicine Resources Conservation State Administration of Traditional Chinese Medicine of the People’s Republic of ChinaInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China

Artemisia annua is the only known plant source of the potent antimalarial artemisinin,which occurs as the low-and high-artemisinin producing(LAP and HAP)***,the different mechanisms of artemisinin producing between these two chemotypes were still not fully ***,we performed a comprehensive analysis of genome resequencing,metabolome,and transcriptome data to systematically compare the difference in the LAP chemotype JL and HAP chemotype *** analysis revealed that 72.18%of sesquiterpenes was highly accumulated in HAN compared to *** omics analysis found a DBR2-Like(DBR2L)gene may be involved in artemisinin ***2L was highly homologous with DBR2,belonged to ORR3 family,and had the DBR2 activity of catalyzing artemisinic aldehyde to dihydroartemisinic *** resequencing and promoter cloning revealed that complicated variations existed in DBR2L promoters among different varieties of *** and were clustered into three variation *** promoter activity of diverse variant types showed obvious ***,the core region(-625 to 0)of the DBR2L promoter was identified and candidate transcription factors involved in DBR2L regulation were ***,the result indicates that DBR2L is another key enzyme involved in artemisinin *** promoter variation in DBR2L affects its expression level,and thereby may result in the different yield of artemisinin in varieties of *** provides a novel insight into the mechanism of artemisinin-producing difference in LAP and HAP chemotypes of ***,and will assist in a high yield of artemisinin in ***.

关键词： thereby insight homologous

Single stage complete resection of giant dumbbell lumbar ganglioneuroma: a case report and review of the literature

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Chinese Neurosurgical Journal 2016年第4期2卷 239-244页

作者： Zuowei wang xingwen wang Fengzeng Jian Li Chen Department of Neurosurgery Department of Neurosurgery Xuanwu Hospital Capital Medical UniversityDivision of Spine China International Neurological Institute Beijing People's Republic of China Department of Pathology Xuanwu Hospital Capital Medical UniversityBeijing People's Republic of China

Background;Ganglioneuroma is a rare benign tumor,which is usually located in the posterior mediastinum and ***,it involves the paraspinal region with intraspinal extension and becomes dumbbell *** ganglioneuromas rarely affect the lumbar spine,and only nine cases of dumbbell lumbar ganglioneuromas have been reported *** presentation;We are reporting an extremely rare case of a giant dumbbell tumor in the lumbar *** performed a one stage total resection of the tumor with posterior approach and long-segment fixation and *** approach is different from previous *** examination of the surgical specimen confirmed the diagnosis of *** months follow up showed a satisfactory ***:Single-stage posterior tumor resection with fixation and fusion is effective and an appropriate choice for the treatments of lumbar dumbbell ganglioneuromas.

关键词： Ganglioneuroma Dumbbell Lumbar spine Surgery

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单分子荧光成像研究单颗粒纳米催化机制

化学学报 2023年第8期81卷 1002-1014页

作者：王晓王星文肖乐辉南开大学化学学院天津300071 中南大学化学化工学院长沙410083

纳米颗粒通常具有优异的催化性能,但由于其内在的异质性,宏观水平的表征难以确定单个纳米颗粒可靠的构效关系和潜在的催化反应机制.单分子荧光成像技术具有单分子灵敏度、高时空分辨率的优点,可以在单颗粒水平实现反应产物的超灵敏检测... 详细信息

纳米颗粒通常具有优异的催化性能,但由于其内在的异质性,宏观水平的表征难以确定单个纳米颗粒可靠的构效关系和潜在的催化反应机制.单分子荧光成像技术具有单分子灵敏度、高时空分辨率的优点,可以在单颗粒水平实现反应产物的超灵敏检测,因而在纳米催化领域得到了广泛应用.本文综述了单分子荧光成像的发展以及该技术在揭示单颗粒纳米催化反应机制中的应用,主要包括尺寸效应、晶面效应、表面缺陷、等离激元效应、双金属效应、活化能、纳米限域效应以及单颗粒催化通讯等方面.最后总结和展望了单分子荧光成像技术在纳米催化研究中的挑战与发展方向.

关键词：单分子荧光单颗粒时空分辨率纳米催化机制构效关系

Reconsideration of the transoral odontoidectomy in complex craniovertebral junction patients with irreducible anterior compression

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中华神经外科杂志(英文) 2021年第1期6卷 28-35页

作者： wang xingwen Ma Longbing Liu Zhenlei Chen Zan Wu Hao Jian Fengzeng Department of Neurological Surgery Xuanwu Hospital Capital Medical University Beijing 100053 China

Background: Although the single-stage posterior realignment craniovertebral junction (CVJ) surgery could treat most of the basilar invagination (BI) and atlantoaxial dislocation (AAD), there are still some cases with incomplete decompression of the spinal cord, which remains a technique challenging ***: Eleven patients were included with remained myelopathic symptoms after posterior correction due to incomplete decompression of the spinal cord. Transoral odontoidectomy assisted by image-guided navigation and intraoperative CT was performed. Clinical assessment and image measurements were performed preoperatively and at the most recent ***: Eleven patients were followed up for an average of 47 months. Symptoms were alleviated in 10 of 11 patients (90.9%). One patient died of an unknown reason 1 week after the transoral approach. The clinical and radiological parameters pre- and postoperatively were ***: Transoral odontoidectomy as a salvage surgery is safe and effective for properly selected BI and AAD patients after inadequate indirect decompression from posterior distraction and fixation. Image-guided navigation and intraoperative CT can provide precise information and accurate localization during operation, thus enabling complete resection of the odontoid process and decompression of the spinal cord.

关键词： Transoral approach Odontoidectomy Basilar invagination Atlantoaxial dislocation Image-guided navigation

城市轨道交通车辆车外辐射噪声关键影响参数研究

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城市轨道交通研究 2020年第6期23卷 68-71,76页

作者：王兴文梁汝军中车南京浦镇车辆有限公司南京210031

以某城市轨道车辆为研究对象,对车辆主要噪声激励源进行调研,并基于几何声线法建立了车辆辐射噪声预测模型。以车辆主要噪声激励源调研结果作为输入,分析讨论了激励源大小对车辆车外辐射噪声影响的量化关系。研究表明:车辆车外辐射噪声... 详细信息

以某城市轨道车辆为研究对象,对车辆主要噪声激励源进行调研,并基于几何声线法建立了车辆辐射噪声预测模型。以车辆主要噪声激励源调研结果作为输入,分析讨论了激励源大小对车辆车外辐射噪声影响的量化关系。研究表明:车辆车外辐射噪声受轮轨噪声激励源影响最显著(轮轨噪声每增大1 dB(A),其辐射噪声增大约0.9~1 dB(A));齿轮箱噪声和牵引电机噪声,受电弓噪声受到的影响最小。

关键词：城市轨道交通车辆辐射噪声轮轨激励几何声线法

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川西页岩-砂岩交互水平井压裂参数优化设计

石油学报 2014年第3期35卷 511-518页

作者：郭建春苟波任山刘林王兴文西南石油大学油气藏地质及开发工程国家重点实验室四川成都610500 中国石油化工股份有限公司西南油气分公司工程技术研究院四川德阳618000

针对川西须家河组五段页岩-砂岩交互及埋藏深情况下的水平井无成熟压裂优化设计方法可借鉴的难题,以XYHF-2井为例,从脆性指数、天然裂缝发育程度、地应力等方面分析了水力裂缝形态;将压后复杂的水力裂缝形态等效为高渗透带,采用数值模... 详细信息

针对川西须家河组五段页岩-砂岩交互及埋藏深情况下的水平井无成熟压裂优化设计方法可借鉴的难题,以XYHF-2井为例,从脆性指数、天然裂缝发育程度、地应力等方面分析了水力裂缝形态;将压后复杂的水力裂缝形态等效为高渗透带,采用数值模拟方法以累积产量为目标优选了高渗透带特征参数;根据等效渗流原理,建立了高渗透带特征参数、岩石力学参数、实验测试导流能力和压裂规模的数学关系模型,并确定了各段压裂规模。研究结果表明:砂岩段容易形成中等水平复杂性的裂缝系统,页岩段容易形成低水平复杂性的裂缝系统;高渗透带特征参数对累积产量的影响程度从大到小依次为:高渗透带数量、高渗透带渗透率和高渗透带长度,XYHF-2井最优的高渗透带数量为10段,渗透率为1.5mD,单翼长度为160m。现场应用结果表明,该设计方法符合须家河组五段地质特征,取得了工区非常规储层的勘探开发突破。该设计方法可以进一步推广至须家河组五段后续压裂井的方案设计。

关键词：页岩-砂岩交互体积压裂复杂裂缝高渗透带水平井优化设计

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裂缝性油藏加砂压裂压力递减分析研究及应用

钻采工艺 2005年第6期28卷 49-51页

作者：王兴文郭建春赵金洲王世泽西南石油学院 "油气藏地质及开发工程"国家重点实验室.西南石油学院中石化西南分公司工程设计研究院

现有的压降分析解释技术只适合于低渗透均质油藏,而不能解释裂缝性油藏的压裂裂缝参数。为了克服传统压降分析解释技术中的不足,在前人研究的基础上,发展和完善了水力压裂压降分析方法,提出了适合裂缝性油藏的压降分析理论和解释技术,... 详细信息

现有的压降分析解释技术只适合于低渗透均质油藏,而不能解释裂缝性油藏的压裂裂缝参数。为了克服传统压降分析解释技术中的不足,在前人研究的基础上,发展和完善了水力压裂压降分析方法,提出了适合裂缝性油藏的压降分析理论和解释技术,并研制了相应的解释软件。该软件可较好地用于裂缝性油藏的压裂压力降落分析,可以解释获得一些重要的裂缝及储层参数,如裂缝滤失系数、初滤失系数、闭合压力、闭合时间、延伸时间、裂缝长度、裂缝宽度,以及压裂液效率等。通过实例计算,说明了解释模型、方法具有一定的可靠性和实用性,这对裂缝性油藏压裂裂缝参数的解释,以及运用获得的参数进行压裂施工效果评价和压裂设计指导具有重要的意义。

关键词：水力压裂裂缝性油藏压降分析解释方法 G函数