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Testing of Seedborne Fungi in Wheat Germplasm Conserved in the National Crop Genebank of China

Agricultural Sciences in China 2007年第6期6卷 682-687页

作者： DUAN Can-xing wang xiao-ming ZHU zhen-dong WU xiao-fei Institute of Crop Sciences Chinese Academy of Agricultural Sciences~The National Key Facilities for Crop Gene Resources and GeneticImprovement （NFCRI） Beijing 100081 P.R.China

There is a primary understanding of the dominant fungi in wheat seeds conserved in the National Crop Genebank of China （NCGB） and an evaluation of the healthy status of wheat germplasm propagated in different regions. A total of 1 465 wheat accessions were detected for seedbone fungi by blotter, agar plate, and wash tests. By blotter test, 17 genera of fungi, including more than 30 species, were detected in 712 wheat accessions from Shaanxi, Hebei, and Qinghai provinces, China. Alternaria was the most frequently detected in wheat seeds from Shaanxi Province, followed by Rhizopus, Penicillium, Aspergillus, Bipolaris, Cladosporium, Gonatobotrys, Chaetomium, and others. Seedborne fungi in wheat seeds from Hebei Province, with relatively high incidence were Alternaria, Rhizopus, Penicillium, Aspergillus, Bipolaris, Cladosporium, and Fusarium. In the seeds from Qinghai Province, Alternaria, Rhizopus, Bipolaris, Cladosporium, and Trichothecium are important seed-borne fungi. The seed germination was reduced substantially when seeds were infected by Fusarium verticillioides （syn. F. moniliforme）, Bipolaris nodulosa, and Cladosporium herbarum. Eighteen genera and 25 species of fungi were identified in 353 accessions from Shaanxi Province using the agar plate test. The dominant fungi were Alternaria, Aspergillus, Bipolaris, Gonatobotrys, Penicillium, and Fusarium. The smut fungi, Ustilago tritici, was detected by the wash test in 400 accessions, but it was low in incidence in 300 seed samples from Shaanxi Province （1.3%）, and in 100 samples from Hebei Province （2.0%）. Totally 19 genera of fungi were detected in wheat seed samples, and some seedborne fungi were saprophytic and others were biotrophic which could cause seedborne diseases in the field.

关键词： wheat gerrnplasm seedborne fungi seed health detection

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A novel real-time RT-PCR with TaqM an-MGB probes and its application in detecting BVDV infections in dairy farms

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Journal of Integrative Agriculture 2015年第8期14卷 1637-1643页

作者： ZHANG Yong-qiang LIU Hai-sheng WU xiao-dong wang xiao-zhen LI Jin-ming ZHAO Yong-gang Lü Yan REN Wei-jie GE Sheng-qiang wang Zhi-liang China Animal Health and Epidemiology Center Beijing Dabeinong Technology Group Co. Ltd.

A real-time RT-PCR assay using Taq Man-MGB probes was developed to detect and type the bovine viral diarrhea virus（BVDV） in *** primers and Taq Man-MGB probes were designed from the 5′-untranslated region of known pestiviral *** to optimizing the assay, c RNAs were transcribed in vitro from the BVDV 1 and BVDV 2 RTPCR products to make standard *** detection limit of the assay was 1.72×102 copies for BVDV 1 and 2.14×102copies for BVDV *** specificity of the assay evaluated on several BVDV strains including bovine herpesvirus 1（BHV 1）, foot and mouth disease virus（FMDV） and several classical swine fever virus（CSFV） strains showed specific detection of the positive virus over 40 *** assay was highly reproducible with the coefficient of variance ranging from 1.04 to 1.33% for BVDV 1 and from 0.83 to 1.48% for BVDV 2, *** this method, we tested a total of 2 327 cattle from three dairy farms for the presence of BVDV persistently infected（PI） *** this assay, each RT-PCR template contained a mixture of ten samples from different *** occurrence rate of PI cattle in three farms ranging from 0.9 to 2.54% could represent partly the PI rates in cattle farm in *** conclusion, using our real-time PCR assay, we could effectively detect and type BVDV and identify PI cattle in a rapid and cost-effective manner.

关键词： bovine viral diarrhea virus(BVDV) real-time RT-PCR persistently infected(PI) animals Taq Man-MGB occurrence rate of PI cattle

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Modified trabeculotomy for primary congenital glaucoma

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Chinese Medical Journal 2013年第19期126卷 3793-3794页

作者： WAN Xiu-hua wang xiao-zhen wang Ning-li Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Science Key Laboratory Beijing 100710 China Beijing Institute of Ophthalmology Beijing 100710 China Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Science Key Laboratory Beijing 100710 China

In traditional trabeculotomy,a radial incision is performed at the corneoscleral transition zone to locate the Schlemm＇s ***,in some conditions,finding the Schlemm＇s canal is difficult because of the enlargement of the comea,developmental anomalies of the angle,*** our study,we performed a modified trabeculotomy,to locate the Schlemm＇s canal easily and accurately and evaluated the outcomes.

关键词： congenital glaucoma trabeculotomy intraocular pressure

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Reproducibility of RTVue retinal nerve fiber layer thickness and optic nerve head measurements in normal and glaucoma eyes

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Chinese Medical Journal 2010年第14期123卷 1898-1903页

作者： LI Ji-peng wang xiao-zhen Fu Jing LI Shu-ning wang Ning-li Tongren Eye Center Beijing Tongren Hospital Capital Medical University Ophthalmology and Visual Science Key Laboratory China Beijing 100730 China

Background RTVue spectral-domain optical coherence tomography （OCT） is a new, ultra high-speed and high-resolution instrument, potentially to measure the presence of glaucoma or its progression accurately. The objective of this study was to evaluate its reproducibility of retinal nerve fiber layer （RNFL） thickness and optic nerve head （ONH） measurements in normal and glaucoma eyes. Methods This study was an observational clinical study. One eye was selected randomly from each of 89 normal individuals and 63 glaucoma patients in a range of severity. RNFL thickness and ONH were measured 3 times on the same day to determine intrasession variability. The same instrument was used by the same operator for all scans. Intrasession within-subject standard deviation （Sw）, precision （1.96xSw）, coefficient of variation （CVw, 100xSw/overall mean）, and intraclass coefficient （ICC） were calculated to evaluate reproducibility. Results RTVue OCT demonstrated double hump patterns in the RNFL profiles. High reproducibility was observed in all ONH parameters. For normal eyes, the value of ICC ranged between 0.98 and 1.00. For eyes with different extent of glaucoma, it ranged between 0.94 and 1.00. High reproducibility was also observed in RNFL thickness measurements. The values of ICC for averaged RNFL thickness ranged between 0,95 and 1.00 in all cases. For regional parameters, it ranged from 0,94 to 0.98 for normal eyes, 0.94 to 1.00 for mild glaucoma eyes, 0.87 to 1.00 for moderate glaucoma eyes, and 0.77 to 0.97 for severe glaucoma eyes. The nasal regions of severe glaucoma appeared to be most variable, as nasal lower region and inferior nasal region had the ICC values of 0.77 and 0.87. Conclusion Reproducibility of RTVue RNFL and ONH measurements was excellent in normal and glaucoma groups.

关键词： diagnostic techniques ophthalmological, instrumentation optical coherence tomography standards reproducibility

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Dual modulation of homogeneous nanomaterialization and electrochemical activation enhancing zinc ion storage

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Science China Chemistry 2025年第1期68卷 385-393页

作者： Jianwei wang Huan wang Kangning wang Wenhui wang Wenlin Zhang Yanzhong zhen School of Chemistry & Chemical Engineering Yan'an University

Vanadium-based electrode materials are widely investigated,but the low specific capacity and slow electrochemical kinetics in aqueous zinc-ion batteries still limit their commercial ***,the VS2/Ca V4O9material with the morphology of nanoflower was synthesized by a one-step hydrothermal *** to the blocky structure of pure VS2material,the VS2/Ca V4O9material is composed of thinner homogeneous *** open structures could provide abundant electrochemical active sites and ion transport channels,and then promote the electrochemical reaction *** addition,they can also buffer the bulk strain during the reaction *** improve the utilization of vanadium elements,an in-situ electrochemical activation strategy is used to explore the storage performance of the VS2/Ca V4O9material,the different activation voltage range of 0.4–1.6 and 0.4–1.4 V are selected,*** with the longer activation plateau of activated-VS2,the VS2/Ca V4O9cathode could quickly reach the activation state in the range of 1.4–1.6 V and cause the release of additional Zn storage sites *** VS2/Ca V4O9cathode delivers a higher power density of 37,000 W kg-1and a significant energy density of 423 Wh *** the high current density of 15 A g-1,the VS2/Ca V4O9cathode still has a discharge capacity of 183.9m Ah g-1after 5,000 cycles,and the capacity decay rate per cycle is only 0.0042%.Continuous cyclic voltammetry （CV） curves,electrochemical impedance spectroscopy （EIS） measurements,density functional theory （DFT） calculation and galvanostatic intermittent titration technique （GITT） measurements demonstrate that the VS2/Ca V4O9cathode has a faster ion diffusion/charge transfer ***,the assembled flexible device has an excellent mechanical stability.

关键词： dual modulation homogeneous nanomaterialization electrochemical activation zinc ion storage

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顶空-气相色谱法分析水中的挥发性苯系物

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石油化工 2001年第1期30卷 48-50页

作者：王小珍茂名石化乙烯工业公司中心化验室广东茂名525011

对顶空 -气相色谱法分析水中的苯、甲苯、乙苯、间二甲苯 ,对二甲苯、邻二甲苯等挥发性苯系物的方法进行了研究 ,并讨论了平衡时间、气 /液体积比、水中含盐量等因素对测定的影响。结果表明 ,方法的回收率为 91%～ 98%、相对标准偏差为 ... 详细信息

对顶空 -气相色谱法分析水中的苯、甲苯、乙苯、间二甲苯 ,对二甲苯、邻二甲苯等挥发性苯系物的方法进行了研究 ,并讨论了平衡时间、气 /液体积比、水中含盐量等因素对测定的影响。结果表明 ,方法的回收率为 91%～ 98%、相对标准偏差为 0 4%～ 3 2 %、最小检测限为 0 0 5～ 2 0 μg/L。该方法准确、快速。

关键词：顶空-气相色谱法挥发性苯系物废水测定

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Effects of HepⅡ domain peptides Ⅴ of fibronectin on corneal permeability, endothelial cells, intraocular pressure and morphology of trabecular meshwork in rats

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Chinese Medical Journal 2011年第13期124卷 2015-2020页

作者： ZHANG Qing-wei wang Ning-li LIU Xu-yang CHEN Feng-hua LI Ping-yu wang xiao-zhen Tongren Eye Center Beijing Tongren Hospital Capital MedicalUniversity Beijing 100730 China

Background Trabecular meshwork （TM） cell volume may be an important determinant of aqueous humor outflow in the eye. This study aimed to evaluate the role of Hepll domain peptides V on corneal permeability, corneal endothelial cells, intraocular pressure （lOP） and morphology of trabecular meshwork in rats. Methods The lOP of rat eyes was measured before and 3, 5, 7 and 8 hours after topical delivery of Hepll domain peptides V through intracameral injections. The peptide＇s concentration in aqueous humor was assessed by high performance liquid chromatography （HPLC）. The shape and density of endothelial cells were observed by laser confocal microscopy 8 hours, 3 and 14 days after intracameral injections of Hepll domain peptides V. The morphological changes in TM of rat eyes were assessed by transmission electron microscopy （TEM）. Results Intracameral injection of Hepll domain peptides V significantly （P 〈0.001） decreased lOP by （5.71±2.10） mmHg in rats at 5 hours after injection. There were no obvious changes of the shape and the density of corneal endothelial cells. In addition, morphological changes in the TM of rats were observed including the expansion of intercellular spaces in the juxtacanalicular meshwork, removal of extracellular material, cellular relaxation, and cytoskeleton reorganization. Conclusions Hepll domain peptides V could not penetrate cornea and was safe to corneal endothelial cells. Hepll domain peptides V could significantly decrease lOP in rat probably by disorganizing actin cytoskeleton and cell-junction in the TM.

关键词： HepH domain peptides V intraocular pressure rat eyes trabecular meshwork high performance liquid chromatography laser confocal microscopy

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A promising approach for quantifying focal stroke modeling and assessing stroke progression:optical resolution photoacoustic microscopy photothrombosis

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Neural Regeneration Research 2025年第7期20卷 2029-2037页

作者： xiao Liang Xingping Quan xiaorui Geng Yujing Huang Yonghua Zhao Lei Xi zhen Yuan Ping wang Bin Liu Faculty of Health Sciences University of MacaoMacao Special Administrative RegionChina Center for Cognitive and Brain Sciences University of MacaoMacao Special Administrative RegionChina Department of Biomedical Engineering Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaipaMacao Special Administrative RegionChina Department of Emergency Zhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral ***,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly *** this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed *** observed the molding process in mice of different ages and presented age-dependent vascular embolism ***,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a *** imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.

关键词： age-dependent cerebral cortex ischemic stroke mouse model optical coherence tomography angiography photoacoustic microscopy photothrombosis vascular imaging

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带自适应动态变异和二次变异的差分进化算法

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计算机集成制造系统 2010年第5期16卷 987-993页

作者：俞国燕李鹏何真王筱珍广东海洋大学工程学院广东湛江524025

为解决差分进化算法在解决多目标优化问题时的多样性与收敛性之间的平衡维持难题,首先提出了一种基于自适应动态变异和非支配解二次变异的改进差分进化算法。该算法的核心是将前N代进化的群体多样性值作为多样性判别准则,根据群体多样... 详细信息

为解决差分进化算法在解决多目标优化问题时的多样性与收敛性之间的平衡维持难题,首先提出了一种基于自适应动态变异和非支配解二次变异的改进差分进化算法。该算法的核心是将前N代进化的群体多样性值作为多样性判别准则,根据群体多样性变化情况自适应地选择对应的变异算子产生新个体;其次提出通过对所存档Pareto非支配解进行二次变异来增加新个体解群的优解质量和数量,以同时改进算法的多样性和收敛速度。仿真结果表明,与标准差分进化算法和改进的基于分类排序的Pareto遗传算法相比,所提算法在收敛性、分布性与分散度性能指标上都有较好的表现,多样性和收敛性之间的平衡维持能力则远优于另两种算法。

关键词：差分进化算法多目标优化自适应动态变异二次变异种群多样性

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Measurement of anterior chamber volume with rotating scheimpflug camera and anterior segment optical coherence tomography

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Chinese Medical Journal 2010年第2期123卷 203-207页

作者： FU Jing LI Shu-ning wang xiao-zhen WU Ge-wei MU Da-peng wang Jian wang Ning-li Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology and Visual Science Key Lab Beijing 100730 China Department of Ophthalmology Beijing Shijingshan Hospital Beijing 100038 China Department of Ophthalmology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

Background Measurement of anterior segment parameters plays an important role in diagnosis and treatment of glaucoma. The objective of this study was to evaluate the repeatability and reproducibility of anterior chamber volume （ACV） measurements with rotating scheimpflug camera （RSC） and to examine agreement with anterior segment optical coherence tomography （AS-OCT）. Methods Thirty nine healthy normal subjects were recruited from the Eye Center of Tongren Hospital. ACV was measured using RSC and AS-OCT in a randomly selected eye for each subject. For RSC measurements, both automatic and manual ACV measurements and 2 independent operators＇ ACV measurements were obtained. All subjects were invited for 3 visits within a week to evaluate repeatability and reproducibility of ACV measurement by RSC. Agreement was evaluated between RSC and AS-OCT. Results Good repeatability and reproducibility were found for both automatic and manual ACV measurements obtained by RSC. For intrasession repeatability, coefficient of variation （CVw） and intraclass correlation coefficient （ICC） values for automatic were 3.52% and 0.98; the values for manual were 3.44% and 0.97, respectively. For intersession reproducibility, the respective CVw and ICC values were 3.96% and 0.96. Good agreement was also found in 2 operators for both automatic and manual ACV measurements; nevertheless, poor agreement was found between RSC and AS-OCT （95% confidence interval （CO for agreement of automatic RSC measurement versus AS-OCT were -96.3 to 72.8 pl and 95% CI for agreement of manual RSC measurement versus AS-OCT were between 41.7 to 10.1 #1 ）. Conclusions Both RSC automatic and manual ACV measurements showed good repeatability and reproducibility, and showed comparable agreement between 2 independent operators, but poor agreement was found between RSC and AS-OCT.

关键词： rotating scheimpflug camera anterior segment optical coherence tomography anterior chamber volume

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