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The uptake diversity of soil nitrogen nutrients by main plant species in Kobresia humilis alpine meadow on the Qinghai-Tibet Plateau

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Science China Earth Sciences 2012年第10期55卷 1688-1695页

作者： wang wenying MA YongGui XU Jin wang HuiChun ZHU JinFu ZHOU HuaKun Department of Life and Geography Sciences Qinghai Normal University Xining 810008 China Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810008 China Center for Agricultural Resources Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Shijiazhuang 050021 China

We studied the uptake of ammonium, nitrate, and a variety of amino acids by alpine plant species in the Kobresia humil alpine meadow ecosystem in situ. We examined the extent of niche separation in uptake of N source by different plant species in alpine communities, and investigated the contribution of symbiotically fixed N to the total N in alpine meadow. The results are （1） δ15N natural abundance values of 13 plant species lie between -2.680‰ and 5.169‰, and the scope is 7.849‰. （2） Le- guminous plants, such as Trigonella ruthenica, Gueldenstaedtia diversiffolia, and Oxytyopis ochrocephala, and non-legumi- nous plant Gentiana straminea uptake low amounts of 15N labeled ammonium, nitrate, glycine or aspartate in soil. （3） As far as the plant uptake of organic N is concerned, Kobresia humilis, Poa pratensis, and Gentiuna spathutifolta can effectively uptake organic nitrogen, and about 37%-40% of the nitrogen of these species comes from soil organic nitrogen sources （such as glycine and aspartate）. Stipa aliena can effectively uptake nitrate, and 60% of its nitrogen comes from soil nitrate. Potentilla anserina, Poa pratensis, and Thalictrum alpinum can effectively absorb ammonium in comparason to other plant species in the meadow, and about 25%-27% of the nitrogen in these plants comes from soil ammonium. （4） The contribution of leguminous fixed N to total N is 7.48%-9.26% in Kobresia humilis alpine meadow. （5） These data show many plant species of alpine meadow may effectively utilize dissolved organic nitrogen such as amino acids, and these plants have diverse ways to uptake soil nitrogen in alpine meadows. Based on the results we can partly explain why there are abundant biodiversities and how plants at alpine habitat utilize the limited soil N sources.

关键词： Kobresia humilis alpine meadow 15N tracer technique plant organic nutrition soil nitrogen

Efficient induction of a Her2-specific anti-tumor response by dendritic cells pulsed with a Hsp70L1–Her2_(341–456) fusion protein

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Cellular & Molecular Immunology 2011年第5期8卷 424-432页

作者： Qiang Fu Yanfeng Wu Fang Yan Ning wang wenying wang Xuetao Cao Yajie wang Tao Wan Department of Oncology Changhai Hospital of ShanghaiShanghai 200433China National Key Laboratory of Medical Immunology and Institute of Immunology Second Military Medical UniversityShanghai 200433China

Heat shock proteins(HSPs)have been shown to interact with antigen-presenting cells(APCs),especially dendritic cells(DCs).HSPs act as potent adjuvants,inducing a Th1 response,as well as antigen-specific CD8^(+) cytotoxic T lymphocytes(CTL)via *** previous work has demonstrated that Hsp70-like protein 1(Hsp70L1),a new member of the Hsp70 subfamily,can act as a powerful Th1 adjuvant in a DC-based *** we report the efficient induction of tumor antigen-specific T cell immune response by DCs pulsed with recombinant fusion protein of Hsp70L1 and Her2_(341–456),the latter of which is a fragment of Her2/neu(Her2)containing E75(a HLA-A2 restricted CTL epitope).The fusion protein Hsp70L1–Her2_(341–456) promotes the maturation of DCs and activates them to produce cytokines,such as IL-12 and TNF-a,and chemokines,such as MIP-1a,MIP-1b and *** together,these results indicate that the adjuvant activity of Hsp70L1 is maintained in the fusion ***2-specific HLA-A2.1-restricted CD8^(+) CTLs can be generated efficiently either from the Peripheral blood lymphocytes(PBL)of healthy donors or from the splenocytes of immunized HLA-A2.1/K^(b) transgenic mice by in vitro stimulation or immunization with DCs pulsed with the Hsp70L1–Her2_(341–456) fusion *** results in more potent target cell killing in an antigen-specific and HLA-A2.1-restricted *** transfer of splenocytes from transgenic mice immunized with Hsp70L1–Her2_(341–456)-pulsed DCs can markedly inhibit tumor growth and prolong the survival of nude mice with Her2^(+)/HLA-A2.1^(+) human *** results suggest that Hsp70L1–Her2_(341–456)-pulsed DCs could be a new therapeutic vaccine for patients with Her2^(+) cancer.

关键词： CTL dendritic cell heat shock protein Her2/neu immunotherapy

Low-dose decitabine enhances the effect of PD-1 blockade in colorectal cancer with microsatellite stability by re-modulating the tumor microenvironment

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Cellular & Molecular Immunology 2019年第4期16卷 401-409页

作者： Ganjun Yu Yanfeng Wu wenying wang Jia Xu Xiaoping Lv Xuetao Cao Tao Wan National Key Laboratory of Medical Immunology&Institute of Immunology Second Military Medical University800 Xiangyin RoadShanghai 200433China

PD-1 blockade has demonstrated impressive clinical outcomes in colorectal cancers that have high microsatellite ***,the therapeutic efficacy for patients with tumors with low microsatellite instability or stable microsatellites needs further ***,we have demonstrated that low-dose decitabine could increase the expression of immune-related genes such as major histocompatibility complex genes and cytokine-related genes as well as the number of lymphocytes at the tumor site in CT26 colorectal cancer-bearing mice.A more significant inhibition of tumor growth and a prolongation of survival were observed in the CT26 mouse model after treatment with a combination of PD-1 blockade and decitabine than in mice treated with decitabine or PD-1 blockade *** anti-tumor effect of the PD-1 blockade was enhanced by low-dose *** results of RNA sequencing and whole-genome bisulfite sequencing of decitabine-treated CT26 cells and tumor samples with microsatellite stability from the patient tumor-derived xenograft model have shown that many immune-related genes,including antigen-processing and antigen-presenting genes,were upregulated,whereas the promoter demethylation was downregulated after decitabine ***,decitabine-based tumor microenvironment re-modulation could improve the effect of the PD-1 *** application of decitabine in PD-1 blockade-based immunotherapy may elicit more potent immune responses,which can provide clinical benefits to the colorectal cancer patients with low microsatellite instability or stable microsatellites.

关键词： colorectal cancer decitabine microsatellite stability PD-1 blockade tumor microenvironment

AI-Enhanced Secure Data Aggregation for Smart Grids with Privacy Preservation

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Computers, Materials & Continua 2025年第1期82卷 799-816页

作者： Congcong wang Chen wang wenying Zheng Wei Gu School of Software Nanjing University of Information Science and TechnologyNanjing210044China School of Information Science and Engineering Zhejiang Sci-Tech UniversityHangzhou310018China State Key Laboratory of Public Big Data Guizhou UniversityGuiyang550025China School of Computer Science and Technology(School of Artificial Intelligence) Zhejiang Sci-Tech UniversityHangzhou310018China School of Computer Science(School of Cyber Science and Engineering) Nanjing University of Information Science and TechnologyNanjing210044China

As smart grid technology rapidly advances,the vast amount of user data collected by smart meter presents significant challenges in data security and privacy *** research emphasizes data security and user privacy concerns within smart ***,existing methods struggle with efficiency and security when processing large-scale *** efficient data processing with stringent privacy protection during data aggregation in smart grids remains an urgent *** paper proposes an AI-based multi-type data aggregation method designed to enhance aggregation efficiency and security by standardizing and normalizing various data *** approach optimizes data preprocessing,integrates Long Short-Term Memory(LSTM)networks for handling time-series data,and employs homomorphic encryption to safeguard user *** also explores the application of Boneh Lynn Shacham(BLS)signatures for user *** proposed scheme’s efficiency,security,and privacy protection capabilities are validated through rigorous security proofs and experimental analysis.

关键词： Smart grid data security privacy protection artificial intelligence data aggregation

Unique genes carried by abundant species enhance CH_(4) emissions during the growing season at the Tibetan Plateau

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Soil Ecology Letters 2024年第2期6卷 83-95页

作者： Yue Liang Liyuan He Jieying wang Yanfang Liu wenying wang Chengjie Ren Jun wang Yaoxin Guo Ninglian wang Fazhu Zhao Shaanxi Key Laboratory of Earth Surface System and Environmental Carrying Capacity Northwest UniversityXi’an710127China College of Urban and Environmental Sciences Northwest UniversityXi’an710127China Department of Biology San Diego State UniversitySan DiegoCA92182USA Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization in Qinghai-Tibet Plateau School of Life SciencesQinghai Normal UniversityXining810008China College of Agronomy Northwest A&F UniversityYangling712100China The College of Life Sciences Northwest UniversityXi’an710072China Carbon Neutrality College (Yulin) Northwest UniversityXi’an710127China

CH_(4) emission rates followed an increased pattern during the growing season at Tibetan Plateau.•Unique genes carried by abundant species were positively correlated with CH_(4) emission rates.•Climate factors influenced CH_(4) emission rates by regulating microbial community and their *** play pivotal roles in soil methane(CH_(4))emissions and their functional genes are origins of a key mechanism for soil ***,understanding of the roles of specific genes(e.g.,unique or shared genes carried by species)underlying CH_(4)-cycling remains ***,we measured CH_(4) emission rates and investigated variations in microbial community and the abundance of genes carried by species during the growing season in alpine meadow on the Tibetan *** discovered that CH_(4) emission rates increased from 394.4,745.9,and 1092.7µg CH4 m−2 h−1,in April,June,and August,respectively,and had a positive correlation with unique genes carried by abundant species during the growing ***,we found that unique genes carried by abundant species involved in methanogenesis processes have a higher abundance than methanotrophic *** analysis indicated that climate factors(i.e.,mean monthly temperature(MMT)and mean monthly precipitation(MMP))influenced microbial community and their functional genes,and therefore affected the CH_(4) emission ***,the present study provides a novel insight into the variation of soil CH4 emissions from a functional gene perspective,highlighting the important roles of unique genes carried by abundant species in CH4 emissions in the Tibetan Plateau under seasonal variation.

关键词： soil CH_(4) emissions unique genes abundant species CH_(4)-cycling growing season

Correction to: Low-dose decitabine enhances the effect of PD1 blockade in colorectal cancer with microsatellite stability by re-modulating the tumor microenvironment

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Cellular & Molecular Immunology 2020年第1期17卷 111-112页

作者： Ganjun Yu Yanfeng Wu wenying wang Jia Xu Xiaoping Lv Xuetao Cao Tao Wan National Key Laboratory of Medical Immunology & Institute of Immunology Second Military Medical University 800 Xiangyin Road 200433 Shanghai China

In the published version of this paper,we repeatedly copied the image of the DAC-7 day as the DAC-14 *** corrected Fig.6 is provided *** figure legend was revised in order to avoid confusion and is shown below for *** we regret our mistake during figure assembly and would like to apologize for any inconvenience it may have caused,we did not manipulate our data in any *** unintentional error also has no bearing on the work's scientific conclusions.

关键词： PD1 colorectal repeatedly

PtHAK5, a candidate for mediating high-affinity K^+ uptake in the halophytic grass, Puccinellia tenuiflora

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Frontiers of Agricultural Science and Engineering 2018年第1期5卷 108-117页

作者： Haili YANG Weidan ZHANG Weiwei CHAI wenying wang Li GAO Jing ZHANG Yongping wang Suo-Min wang State Key Laboratory of Grassland Agro-ecosystems/College of Pastoral Agriculture Science and Technology Lanzhou University

Puccinellia tenuiflora is a typical salt-excluding halophytic grass with strong salt-tolerance, which enhances tolerance by restricting Na^+influx as well as having a strong selectivity for K^+ over Na^+. The HAK5 K^+ transporters generally modulate effective K^+acquisition in plants, especially under low K^+ condition. In this study,Pt HAK5 from P. tenuiflora was isolated by RT-PCR and characterized using yeast complementation. The results showed Pt HAK5 consisted of 784 amino acids and shared over 80% homology with the identified high-affinity K^+ transporter HAK5 from other higher plants. The expression of PtHAK5 rescued the K^+ -uptake-defective phenotype of yeast strain CY162. In conclusion, PtHAK5 is a candidate for mediating high-affinity K^+ uptake under low K^+ conditions.

关键词： K+ uptake PtHAK5 Puccinellia tenuiflora yeast complementation

Will Glacier No.1 Tianshan exist in the 22^(nd) century?

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Research in Cold and Arid Regions 2011年第3期3卷 206-215页

作者： wenying wang YongPing Shen Chang＇an University Xi＇an Shaanxi 710054 China Cold and Arid Regions Environmental and Engineering Research Institute Chinese Academy of Sciences Lanzhou Gansu 730000 China

Mountains have been described as the water towers of the world. Almost all major rivers have their sources in mountains; glaciers are important water resources that contribute meltwater to river discharge. Glaciers participate in the global water cycle and, with their solid water storage, are an important component of the water balance. As solid reservoirs, glaciers continue to receive the mass nourishment of solid precipitation from the atmosphere, and their meltwater feed and regulate fiver discharge. Physical changes in glaciers are an indicator of climate change. Over the past half century, the global temperature has increased by 1-2 ℃, which emphasizes the urgent task of monitoring glaciers and predicting their trend. As an example, we have investigated, researched, and surveyed Glacier No. 1 in the Urumqi River source, Tianshan （abbr. Glacier No. 1 Tianshan or Glacier No.l） for half a century. We have found an increase by degrees of the glacial regression during the last 400 years and discovered a terminal moraine which is forming today. The global temperature is rising continually, while the local glacial temperature is 0.4 times that of the global temperature change. Thus, we forecast that Glacier No. 1 Tianshan will disappear during the late 21 st Century （2074-2100 A.D.）.

关键词： glacier retreat change forecast

Total mesh excision via divergent approaches:Two case reports and literature review

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Gynecology and Obstetrics Clinical Medicine 2023年第3期3卷 181-183页

作者： wenying wang Yongxian Lu Department of Obstetrics and Gynecology Fourth Medical CenterGeneral Hospital of People's Liberation ArmyBeijingChina

Dear Editor,The introduction of mesh procedures to treat stress urinary incontinence(SUI)in women represents a significant milestone breakthrough and currently serves as the primary surgical approach for addressing *** the FDA's statement on mesh sling complications in 2008,an increasing number of patients have sought total excision of the polypropylene(PP)sling from their ***,there are a few reports on PP sling removal through different routes,but a unified and specific surgical technique has not yet been *** aim of this article is to report the successful techniques and approaches used in two cases involving the total removal of PP meshes resulting from tension-free vaginal tape(TVT)and transobturator tape(TOT)via different ***,we discuss the clinical outcomes and review relevant literature on this topic.

关键词： excision urinary breakthrough