检索结果-南通市图书馆

Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency

Journal of Integrative Medicine 2024年第1期22卷 72-82页

作者： Jing-shun Meng Yun He Heng-bin Yang Li-ping Zhou si-yuan wang Xi-lin Feng Omar Yahya Al-shargi Xiao-min Yu Li-qing Zhu Chang-quan Ling Shanghai University of Traditional Chinese Medicine Shanghai 201203China Oncology Department of Traditional Chinese Medicine Changhai HospitalNaval Medical UniversityShanghai 200433China Faculty of Traditional Chinese Medicine Naval Medical UniversityShanghai 200043China State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(Ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Yantai Fuheng Biological Technology Co. Ltd.YantaiShandong Province 264006China. Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

Objective Melittin and its derivatives have been characterized to establish effective gene delivery *** capability of facilitating endosomal release enhances the nanoparticles-based gene ***,little investigation has been conducted to explore its potential application in the context of viral *** Various melittin-derived peptides were inserted into the loop VIII of the capsid proteins of recombinant adeno-associated virus *** vectors carrying either gfp or fluc genes were subjected to qPCR assays and transduction assays of HEK293T cells to investigate the efficiency of vector production and gene *** addition,the ability of a specific p5RHH-rAAV vector to deliver genes was examined through in vitro transduction of different cultured cells and in vivo tail vein administration to C57BL/6 ***,the intricate details of the vector-mediated transduction mechanisms were revealed by specific pharmacological inhibitors of every stage of the rAAV2 intracellular life *** A total of 76 melittin-related peptides were compiled from existing *** them,cMA2,Melt13,p5RHH and aAR3 were found to significantly enhance the gene delivery efficiency of rAAV2 *** p5RHH-rAAV2 vectors efficiently transduced not only rAAV-potent cell lines but also cell lines previously considered resistant to ***,bafilomycin A1,a vacuolar endosome acidification inhibitor,completely inhibited the transgene expression mediated by the p5RHH-rAAV2 *** importantly,p5RHH-rAAV8 vectors also demonstrated increased hepatic transduction in vivo in C57BL/6 *** The incorporation of melittin analogues into the rAAV capsids results in a significant improvement in rAAV-mediated transgene *** further modifications remain an area of interest,our studies have substantially broadened the pharmacological prospects of melittin in the context of viral vector-mediated gene delivery.

关键词： Melittin Recombinant adeno-associated virus Capsid engineering Transduction efficiency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Analytical Solution and simplified Formula for Added Mass of Horizontal and Vertical Motions of Truncated Cylinders Under Earthquake Action

引用

China Ocean Engineering 2024年第1期38卷 54-67页

作者： wang Pi-guang LYU si-yu QU Yang ZHAO Mi DU Xiu-li State Key Laboratory of Bridge Engineering Safety and Resilience Beijing University of TechnologyBeijing 100124China Key Laboratory of Urban Security and Disaster Engineering of Ministry of Education Beijing University of Tech-nologyBeijing 100124China

This paper investigates the hydrodynamic characteristics of floating truncated cylinders undergoing horizontal and vertical motions due to earthquake excitations in the finite water *** governing equation of the hydrodynamic pressure acting on the cylinder is derived based on the radiation theory with the inviscid and incompressible *** governing equation is solved by using the method of separating variables and analytical solutions are obtained by assigning reasonable boundary *** analytical result is validated by a numerical model using the exact artificial boundary simulation of the infinite *** main variation and distribution characteristics of the hydrodynamic pressure acting on the side and bottom of the cylinder are analyzed for different combinations of wide-height and immersion *** added mass coefficient of the cylinder is calculated by integrating the hydrodynamic pressure and simplified formulas are proposed for engineering *** calculation results show that the simplified formulas are in good agreement with the analytical solutions.

关键词： earthquake hydrodynamic pressure truncated cylinder added mass simplified formula

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

BL03HB: a Laue microdiffraction beamline for both protein crystallography and materials science at SSRF

引用

Nuclear Science and Techniques 2024年第7期35卷 68-78页

作者： Zhi-Jun wang si-Sheng wang Zheng-Huang Su Li Yu Yu-Zhu wang Bo Sun Wen Wen Xing-Yu Gao Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai201210China University of Chinese Academy of Science Beijing100049China

A Laue microdiffraction beamline (BL03HB) was constructed at the Shanghai SynchrotronRadiation Facility (SSRF). This beamline featu res two consecutive focusing points in twodifferent sectors within its end station, the first dedicated to protein crystallography and theother tailored to materials science applications. Based on a superbend dipole magnet with amagnetic field of 2.29 T, a two-stage focusing design was implemented with two sets of Kirkpatrick- Baez mirrors to achieve a micro white beam as small as 4.2 um X4.3 pμm at the firstsector and 09 pum X 1.3 pum at the second sector in the standard beamline operation mode at *** X-ray microbeam in the two sectors can be easily switched between monochromatic andwhite beams by moving a four-bounce monochromator in or out of the light path, respectively. Inthe protein crystallography sector, white-beam Laue microdifraction was dermonstrated tosuccessully determine the structure of protein crystals from only a few images of diffraction data .collected by a Pilatus 2 M area detector. In the materials science sector, the white -beam Lauediffraction was collected in a reflection geometry using another Pilatus 2 M area detector, whichcould map the microstructural distribution on the sample surface by scanning the samples. Ingeneral, the BL03HB beamline promotes the application of Laue microdiffraction in both proteincrystallgraphy and materials science. This paper presents a comprehensive overview of theBL03HB beamline, end station, and the first commission results.

关键词： Crystallography White-beam Laue microdiffraction Beamline Synchrotron

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Beam based alignment using a neural network

引用

Nuclear Science and Techniques 2024年第4期35卷 108-118页

作者： Guan-Liang wang Ke-Min Chen si-Wei wang Zhe wang Tao He Masahito Hosaka Guang-Yao Feng Wei Xu National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China Diamond Light Source Oxfordshire OX110DEUK

Beams typically do not travel through the magnet centers because of errors in storage *** beam deviating from the quadrupole centers is affected by additional dipole fields due to magnetic field ***-based alignment(BBA)is often performed to determine a golden orbit where the beam circulates around the quadrupole center *** storage rings with many quadrupoles,the conventional BBA procedure is time-consuming,particularly in the commissioning phase,because of the necessary iterative *** addition,the conventional BBA method can be affected by strong coupling and the nonlinearity of the storage ring *** this study,a novel method based on a neural network was proposed to determine the golden orbit in a much shorter time with reasonable *** golden orbit can be used directly for operation or adopted as a starting point for conventional *** method was demonstrated in the HLS-II storage ring for the first time through simulations and online *** results of the experiments showed that the golden orbit obtained using this new method was consistent with that obtained using the conventional *** development of this new method and the corresponding experiments are reported in this paper.

关键词： Golden orbit Beam-based alignment Neural network Storage ring

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interfacial built-in electric field and crosslinking pathways enabling WS_(2)/Ti_(3)C_(2)T_(x) heterojunction with robust sodium storage at low temperature

引用

Journal of Energy Chemistry 2024年第2期89卷 635-645,I0014页

作者： Jiabao Li Shaocong Tang Jingjing Hao quan Yuan Tianyi wang Likun Pan Jinliang Li Shenbo Yang Chengyin wang College of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002JiangsuChina Shanghai Key Laboratory of Magnetic Resonance School of Physics and Electronic Science East China Normal UniversityShanghai 200241China siyuan Laboratory Guangdong Provincial Engineering Technology Research Center of Vacuum Coating Technologies and New Energy MaterialsGuangdong Provincial Key Laboratory of Nanophotonic ManipulationDepartment of PhysicsJinan UniversityGuangzhou 510632GuangdongChina Hongzhiwei Technology(Shanghai)CO.LTD Shanghai 200241China

Developing efficient energy storage for sodium-ion batteries(siBs)by creating high-performance heterojunctions and understanding their interfacial interaction at the atomic/molecular level holds promise but is also ***,sluggish reaction kinetics at low temperatures restrict the operation of siBs in cold ***,cross-linking nanoarchitectonics of WS_(2)/Ti_(3)C_(2)T_(x) heterojunction,featuring built-in electric field(BIEF),have been developed,employing as a model to reveal the positive effect of heterojunction design and BIEF for modifying the reaction kinetics and electrochemical ***,the theoretical analysis manifests the discrepancy in work functions leads to the electronic flow from the electron-rich Ti_(3)C_(2)T_(x) to layered WS_(2),spontaneously forming the BIEF and“ion reservoir”at the heterogeneous ***,the generation of cross-linking pathways further promotes the transportation of electrons/ions,which guarantees rapid diffusion kinetics and excellent structure ***,superior sodium storage performance is obtained for the WS_(2)/Ti_(3)C_(2)T_(x) heterojunction,with only 0.2%decay per cycle at 5.0 A g^(-1)(25℃)up to 1000 cycles and a high capacity of 293.5 mA h g^(-1)(0.1A g^(-1)after 100 cycles)even at-20℃.Importantly,the spontaneously formed BIEF,accompanied by“ion reservoir”,in heterojunction provides deep understandings of the correlation between structure fabricated and performance obtained.

关键词： WS_(2)/Ti_(3)C_(2)T_(x)heterojunction Built-in electric field Ion reservoir Reaction kinetics Sodium storage performance at low temperature

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hydrodynamic Performance of An Integrated System of Breakwater and A Multi-Chamber OWC Wave Energy Converter

引用

China Ocean Engineering 2024年第4期38卷 543-556页

作者： NING De-zhi ZHANG Xiang-yu wang Rong-quan ZHAO Ming State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China Dalian Key Laboratory of Offshore Renewable Energy Dalian University of TechnologyDalian 116024China School of Computing Engineering and MathematicsUniversity of Western SydneyPenrith NSW 2751Australia

A multi-chamber oscillating water column wave energy converter(OWC-WEC)integrated to a breakwater is *** hydrodynamic characteristics of the device are analyzed using an analytical model based on the linear potential flow theory.A pneumatic model is employed to investigate the relationship between the air mass flux in the chamber and the turbine *** effects of chamber width,wall draft and wall thickness on the hydrodynamic performance of a dual-chamber OWC-WEC are *** results demonstrate that the device,with a smaller front wall draft and a wider rear chamber exhibits a broader effective frequency *** device with a chamber-width-ratio of 1:3 performs better in terms of power ***,results from the analysis of a triplechamber OWC-WEC demonstrate that reducing the front chamber width and increasing the rearward chamber width can improve the total performance of the *** the number of chambers from 1 to 2 or 3 can widen the effective frequency bandwidth.

关键词： oscillating water column power extraction efficiency potential flow theory wave energy converter multi-chamber

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multi-objective optimal control of tungiasis diseases with terminal demands

引用

International Journal of Biomathematics 2024年第3期17卷 123-151页

作者： Wei Lv si-Ting Zhang Lei wang Department of Mathematics Shanghai University Shanghai200444P.R.China Key Laboratory for Computational Mathematics and Data Intelligence of Liaoning Province Dalian University of Technology Dalian 116024P.R.China Department of Mathematics School of Science Shihezi UniversityShihezi 832003P.R.China

In this paper,we aim to minimize the epidemic size of tungiasis disease and economic costs simultaneously,with terminal demands for infected humans.A human-jigger parasite control system with four control schemes for humans and jiggers is *** propose a multi-objective optimal control problem with terminal constraints,in which the accumulated number of infected humans and control costs are *** applying the modified normal boundary intersection algorithm and the interior point scheme,numerical simulations for different combinations of control schemes are carried out,and actual data in Madagascar are *** combination schemes are indicated from the perspectives of disease eradication,cost saving and time *** these effective combinations are properly performed,the disease can be *** only minimizing the epidemic size,the combination of the optimal treatments and adulticiding efforts is the best choice in the rainy season;the combination of the optimal personal protections and treatments is the preferential option in the dry *** only minimizing the economical cost,the combination of the optimal adulticide and larvicide is the better selection in the rainy season;the combination of the optimal personal protections,treatments and adulticiding efforts is the prior choose in the dry ***,there is a trade-off between the two objectives for all the effective combinations,decision-makers may choose an appropriate one to control the disease.

关键词： Multi-objective optimal control terminal demand control combination tun-giasis disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Constructing energetic covalent organic frameworks with high stability and low sensitivity

引用

Chinese Chemical Letters 2024年第3期35卷 544-548页

作者： Hui-Min Guo Xiao-Yu Dong Shan wang Qian-You wang Shuang-quan Zang Henan Key Laboratory of Crystalline Molecular Functional Materials Green Catalysis Centerand College of ChemistryZhengzhou UniversityZhengzhou 450001China

Developing new functional explosives that display high stability,good energy performance,and low sensitivity are one of the key directions of energetic materials *** this work,two-dimensional(2D)Schiff-based energetic covalent organic frameworks(COFs)are prepared based on triaminoguanidine salts with different anions as building *** from the robust covalent bond in 2D extended polygons and strongπ-πinteractions in the eclipsed interlayers,the synthesized energetic COFs showed higher thermal stability and lower mechanical sensitivity than their precursor *** importantly,incorporating triaminoguanidine salts into COFs effectively increase the corrosion resistance to metal under high humidity conditions,which is due to the imine moieties in COFs functioning asπacceptors and offering strong bonding with metallic *** work provides a new pathway for the development of high-performance energetic materials.

关键词： Covalent organic frameworks Energetic materials High thermal stability Low sensitivity Corrosion resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bilayer separator enabling dendrite-free zinc anode with ultralong lifespan >5000 h

引用

Green Energy & Environment 2024年第4期9卷 771-776页

作者： Lu wang Feifei wang Zhe Ding Yingxin Liu Ziyi Zhang Chunpeng Yang Kian Ping Loh quan-Hong Yang Joint School of National University of singapore and Tianjin University International Campus of Tianjin UniversityFuzhou350207China Nanoyang Group State Key Laboratory of Chemical EngineeringSchool of Chemical Engineering and TechnologyTianjin UniversityCollaborative Innovation Center of Chemical Science and Engineering(Tianjin)Tianjin300072China Haihe Laboratory of Sustainable Chemical Transformations Tianjin300192China Department of Chemistry National University of Singapore117543Singapore Institute of Process Engineering Chinese Academy of SciencesBeijing100190China

Aqueous zinc(Zn)batteries with Zn metal anodes are promising clean energy storage devices with intrinsic safety and low ***,Zn dendrite growth severely restricts the use of Zn *** effectively suppress Zn dendrite growth,we propose a bilayer separator consisting of commercial butter paper and glassfiber *** dense cellulose-based butter paper(BP)with low zincophilicity and high mechanical properties prevents the pore-filling behavior of deposited Zn and related separator piercing,effectively suppressing the Zn dendrite *** a result,the bilayer separators endow the ZnjjZn symmetrical batteries with a superlong cycling life of Zn anodes(over 5000 h)at 0.5 mA cm^(-2) and the full batteries enhanced capacity retention,demonstrating the advancement of the bilayer separator to afford excellent cyclability of aqueous metal batteries.

关键词： Zn battery Bilayer separator Butter paper Zn metal anode Zn dendrite

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

3D interlocked all-textile structured triboelectric pressure sensor for accurately measuring epidermal pulse waves in amphibious environments

引用

Nano Research 2024年第3期17卷 1923-1932页

作者： Shaobo si Chenchen Sun Yufen Wu Jingjing Li Han wang Yinggang Lin Jin Yang Zhong Lin wang Department of Optoelectronic Engineering Key Laboratory of Optoelectronic Technology and Systems Ministry of EducationChongqing UniversityChongqing 400044China College of Physics and Electronic Engineering Chongqing Normal UniversityChongqing 401331China Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 100083China School of Materials Science and Engineering Georgia Institute of TechnologyAtlantaGA 30332USA

The performance degradation and even damage of the e-textiles caused by sweat,water,or submersion during all-weather health monitoring are the main reasons that e-textiles have not been commercialized and routinized so ***,we developed an amphibious,high-performance,air-permeable,and comfortable all-textile triboelectric sensor for continuous and precise measurement of epidermal pulse waves during full-day *** on the principle of preparing gas by acid-base neutralization reaction,a one-piece preparation process of amphibious conductive yarn(ACY)with densely porous structures is *** innovative three-dimensional(3D)interlocking fabric knitted from ACYs(0.6 mm in diameter)and polytetrafluoroethylene yarns exhibit high sensitivity(0.433 V·kPa^(-1)),wide bandwidth(up to 10 Hz),and stability(>30,000 cycles).With these benefits,98.8%agreement was achieved between wrist pulse waves acquired by the sensor and a high-precision laser ***,the polytetrafluoroethylene yarn with good compression resilience provides sufficient mechanical support for the contact separation of the ***,the unique skeletonized design of the 3D interlocking structure can effectively relieve the water pressure on the sensor surface to obtain stable and accurate pulse waves(underwater depth of 5 cm).This achievement represents an important step in improving the practicality of e-textiles and early diagnosis of cardiovascular diseases.

关键词： triboelectric pressure sensor amphibious fabric interlocking fabric pulse wave measurement one-piece preparation method

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：