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Acta Pharmacologica Sinica 2005年第7期26卷 845-850页

作者： shao-wei CHEN Xiao-fang wang Ying shao hong XUE Li ZHOU Tai YAO Li-min LU~2 Department of Physiology and Pathophysiology,Shanghai Medical College,Fudan University,Shanghai 200032,China Department of Physiology and Pathophysiology Shanghai Medical College Fudan University Shanghai China

Aim:To construct pEGFP-N3 recombinant vectors carrying adrenomedullin(AM)or fragments of the AM gene,and to express AM or fragments of AM from thepEGFP-N3 recombinant vectors(pEGFP-N3-AM 1-2 and pEGFP-N3-AM1-3)andstudy their biological properties on cultured rat renal mesangial cells(RMC).Methods:Total RNA of rat kidney was obtained using TriZol *** cDNAwas synthesized by reverse transcriptase using oligo-deoxythymidine as *** fragments of AM gene were then amplified by polymerase chain reaction(PCR)with specific upstream and downstream *** PCR prod-ucts were digested with EcoRI and BamHI and subcloned into the plasmid *** by cationic liposomes,RMC were transfected with pEGFP-N3-AM1-2 or pEGFP-N3-AM *** 24 h,green fluorescent protein(GFP)fluores-cent images were examined with a fluorescence *** 48 h,the prolif-eration of RMC was detected using the MTT assay,and the mRNA expression oftransforming growthfactor-β1(TGF-β1)was measured by semiquantitative ***:DNA sequence reports verified that pEGFP-N3-AM1-2,which carriedthe full length AM gene translation fragment(preproadrenomedullin preproAM1-185),and pEGFP-N3-AM1-3,which carried the translation fragment of preproAM[without adrenotensin(ADT,preproAM150-185)],were constructed *** 24 h,green fluorescence was observed in RMC into which either pEGFP-N3-AM 1-2 or pEGFP-N3-AM 1-3 was transfected,while in the control cells no fluores-cence was *** pEGFP-N3-AM1-2 or pEGFP-N3-AM1-3 delivery in-hibited the proliferation of RMC(P<0.01)and decreased the mRNA transcriptionlevel of TGF-~I in RMC(P<0.05).However,no significant difference was ob-served between the effects of pEGFP-N3-AM1-2 and ***:pEGFP-N3-AM 1-2 and pEGFP-N3-AM 1-3 were constructed suc-cessfully and were functionally expressed in *** the fragment ofAM without ADT has similar inhibitory biological effects on RMS proliferationand TGF-

关键词： adrenomedullin transfection glomerular mesangium transforming growth factor-β1

Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He Li Li Sai wang shao-Jiang wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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12例急性膝关节后外侧结构损伤的手术治疗

中国骨伤 2012年第5期28卷 400-403页

作者：王绍宏武安市第一人民医院骨科河北武安056300

目的:探讨急性膝关节后外侧结构(posterolateral structures,PLS)损伤的手术方法及疗效。方法:2006年5月至2008年10月收治急性PLS损伤患者12例(12膝),男9例,女3例;年龄23～47岁,平均31岁。其中合并后交叉韧带损伤9例,前后交叉韧带损伤3... 详细信息

目的:探讨急性膝关节后外侧结构(posterolateral structures,PLS)损伤的手术方法及疗效。方法:2006年5月至2008年10月收治急性PLS损伤患者12例(12膝),男9例,女3例;年龄23～47岁,平均31岁。其中合并后交叉韧带损伤9例,前后交叉韧带损伤3例,关节囊撕裂7例。首先在关节镜下探查,修复损伤半月板,前后交叉韧带损伤者与PLS同期重建,根据PLS损伤程度采用异体肌腱解剖重建腘肌腱单位(popliteus muscle tendon unit,PMTU)和(或)外侧副韧带(lateral collateral ligament,LCL),PLS重建完成后进行交叉韧带的重建固定,合并关节囊撕裂的采用局部加强缝合的手术方法治疗,术后根据IKDC及Lysholm功能评定标准评定膝关节功能恢复情况。结果:12例均获随访,时间12～24个月,平均(16.5±3.2)个月。根据IKDC综合评定标准,正常(A级)8膝,接近正常(B级)3膝,异常(C级)1膝。Lysholm膝关节功能评分从术前的(39.6±3.1)分增加到术后的(85.1±2.2)分,患膝红、肿、疼痛均消失。结论:急性PLS损伤需手术解剖重建PMTU、LCL等结构,手术操作的要求精度较高,不仅需要精确地建立骨隧道,还要注意保护腓总神经和避免植入的异体肌腱切割,使重建的膝关节后外侧结构更加稳定。

关键词：膝关节韧带创伤和损伤修复外科手术

Fair Electronic Payment Scheme Based on DSA

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Wuhan University Journal of Natural Sciences 2005年第1期10卷 203-206页

作者： wangshao-bin hongFan ZHUXian CollegeofComputerScienceandTechnology HuazhongUniversityofScienceandTechnologyWuhan430074HubeiChina

We present a multi-signature scheme based on DSA and describes a faireleetronic payment scheme based on improved DSA signatures. The scheme, makes both sides in e-qualpositions during the course of electronic transaction A Trusted Third Party (TTP)is involved in thescheme to guarantee the fairness of the scheme for both sides. However, only during the course ofregistration and dispute resolution will TTP be needed TTP is not needed during the normal paymentstage.

关键词： fair electronic payment multi-signatures DSA signatures E-commerce

Regulation of estrogen receptors α and β in human breast carcinoma by exogenous leptin in nude mouse xenograft model

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Chinese Medical Journal 2010年第3期123卷 337-343页

作者： YU Wei GU Jun-chao LIU Jian-zhong wang shao-hong wang Yu ZHANG Zhong-tao MA Xue-mei SONG Mao-min Department of General Surgery Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of General Surgery Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

Background It is essential to clarify the interactions of hormones during the progression of human breast cancer. This study examined the effects of exogenous human leptin on estrogen receptor （ER） α and β in human breast tumor tissue in a nude mouse xenograft model. Methods We created nude mice xenografts of MCF-7 human breast cancer cells, and randomly divided them into an experimental group and a control group. The mice in experimental group were injected subcutaneously around tumors with human leptin, while the control group were injected with the same dose of normal saline. A real-time RT-PCR assay was developed to quantify the mRNA of ERα, β in the tumor tissues. Western blotting analyses were used to assess the relative quantities of the ERα , β proteins. Results Leptin-treated xenografted nude mice were successfully established. The amount of ERa mRNA was significantly higher in the leptin group than in the control group （P 〈0.01）, while the amount of ERβ mRNA was significantly lower in the leptin group than in the control group （P 〈0.01）. Western blotting analyses revealed that the ERa protein level was significantly higher in the leptin group than in the control group （P 〈0.01）, while the ERβ protein level was significantly lower in the leptin group than in the control group （P 〈0.01）. Conclusions Nude mouse xenograft model can be safely and serviceably treated with human leptin by subcutaneous injections around tumor. ERα, β were both targets of leptin in breast cancer. Leptin can up-regulate the expression of ERa and down-regulate the expression of the ERβ in human breast tumor.

关键词： human leptin xenografl model estrogen receptors α and β

基于小波包惩罚函数的烟机振动信号软阈值降噪

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北京理工大学学报 2010年第8期30卷 906-909,914页

作者：陈涛徐小力王少红北京理工大学机械与车辆学院北京100081 北京信息科技大学现代测控教育部重点实验室北京100192

为解决烟机振动信号受到噪声干扰这一问题,研究基于小波包阈值降噪的原理和方法,给出了小波包阈值降噪的步骤,阐述了Birgé-Massart惩罚函数确定阈值的原则和软阈值的量化处理,分析了阈值、信噪比和均方误差随惩罚因子的变化规律.并将... 详细信息

为解决烟机振动信号受到噪声干扰这一问题,研究基于小波包阈值降噪的原理和方法,给出了小波包阈值降噪的步骤,阐述了Birgé-Massart惩罚函数确定阈值的原则和软阈值的量化处理,分析了阈值、信噪比和均方误差随惩罚因子的变化规律.并将基于小波包惩罚函数的软阈值降噪与Rigrsure、Heursure、Sqtwolog、Minimax4种阈值降噪方法进行了比较.结果表明基于惩罚函数的小波包软阈值方法能有效降低噪声.基于该方法的烟机振动信号降噪在保留信号突变部分的同时,具有良好的光滑性.

关键词：降噪振动信号小波包软阈值惩罚函数

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基于物元模型的小区开放对道路通行的影响

应用科学学报 2017年第5期35卷 612-625页

作者：肖同王卓王梦阎少宏华北理工大学建筑工程学院河北唐山063210 华北理工大学理学院河北唐山063210 河北省数据科学与应用重点实验室河北唐山063210

小区开放对道路通行的影响是社会关注的焦点.基于物元理论建立了车辆通行能力模型,选择北京市富力城和海晟名苑两个典型小区为代表.对不同小区以递进形式分别设计3种开放方案,结合物元模型得到富力城、海晟名苑两个小区的级别变量值分别... 详细信息

小区开放对道路通行的影响是社会关注的焦点.基于物元理论建立了车辆通行能力模型,选择北京市富力城和海晟名苑两个典型小区为代表.对不同小区以递进形式分别设计3种开放方案,结合物元模型得到富力城、海晟名苑两个小区的级别变量值分别为2.2、1.3,由物元理论可知小区开放对周边道路通行有正面影响.模型通过关联函数将一般评价取值的闭区间拓展到实数轴,并可推广应用到环境质量、工农业资源等领域.

关键词：小区开放交通量物元模型交通影响度

碳达峰目标下我国能源转型的现状、挑战与突破

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价格理论与实践 2021年第8期 82-86,172页

作者：王少洪(翻译) 中国政法大学政治与公共管理学院

碳达峰目标彰显出我国应对全球气候变化、推动全球可持续发展的大国担当。能源转型是碳达峰、碳中和目标实现乃至将绿色发展转化为新的综合国力和国际竞争优势的关键环节。本文首先从能源消费和生产总量、能源强度、能源消费结构、能源... 详细信息

碳达峰目标彰显出我国应对全球气候变化、推动全球可持续发展的大国担当。能源转型是碳达峰、碳中和目标实现乃至将绿色发展转化为新的综合国力和国际竞争优势的关键环节。本文首先从能源消费和生产总量、能源强度、能源消费结构、能源生产结构四个方面分析我国能源转型的状况。然后分析碳达峰目标下我国能源转型在经济社会发展阶段、能源低碳转型相关机制、技术发展、资源禀赋四个方面的挑战。最后,提出碳达峰目标下我国能源转型的主要路径和对策建议:要充分发挥社会主义制度优越性;立足于"开源"和"节流";完善相关市场机制,从顶层设计引导、稳定和加速能源低碳转型;以增强科技进步、创新为突破,构建以新能源为主体的新型电力系统。

关键词：碳达峰能源转型碳交易市场新型电力系统

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青岛市落实良种补贴项目的思路与成效

种子 2007年第6期26卷 82-84页

作者：王韶红山东省青岛市种子站山东青岛266108

2005年度,在国家农业部、财政部的大力支持下,我市首次承担了国家下达的"百万亩"优质专用小麦良种推广补贴项目,补贴资金1000万元.项目计划下达后,根据农业部办公厅、财政部办公厅《关于下达2005年国家良种补贴项目实施方案的通知》要... 详细信息

2005年度,在国家农业部、财政部的大力支持下,我市首次承担了国家下达的"百万亩"优质专用小麦良种推广补贴项目,补贴资金1000万元.项目计划下达后,根据农业部办公厅、财政部办公厅《关于下达2005年国家良种补贴项目实施方案的通知》要求,我市认真进行了组织实施.从初步显现的效果看,通过该项目各项具体政策、措施的落实,极大地调动了广大农民的种粮积极性,有力地促进了我市粮食生产尤其是优质专用小麦生产的发展.

关键词：补贴资金良种推广青岛市农业部办公厅优质专用小麦项目计划项目实施财政部