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AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam (Ruditapes philippinarum) in China

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Acta Oceanologica Sinica 2007年第1期26卷 150-158页

作者： LIU Xiangquan BAO Zhenmin HU Jingjie wang Shi ZHAN Aibin LIU Hui FANG Jianguang wang rucai Laboratory of Marine Genetics and Breeding （ MGB） Ocean University of China Qingdao 266003 China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Science Qingdao 266071 China

The amplified fragment length polymorphism （AFLP） technology was used to analyze the genetic diversities in four natural populations of Manila clam （ Ruditapes philippinarum）, distributed in four sea areas of China, i.e. , the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea. Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity. The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon＇ s information index was from 0.256 8 to 0. 275 6. By analyzing molecular variance （ AMOVA）, it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas. Cluster analysis by Nei＇ s pairwise distances grouped specimens by geographical origin, except the population of Qingdao. A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations. The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao.

关键词： Ruditapes philippinarum genetic diversity geographical population AFLP

Histological and Histochemical Analyses of the Cuttlebone Sac of the Golden Cuttlefish Sepia esculenta

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Journal of Ocean University of China 2007年第4期6卷 393-397页

作者： ZHENG Xiaodong XIAO Shu wang Zhaoping wang rucai The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China Guangdong Key Laboratory of Applied Marine Biology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 P. R. China

The secretion function of mantle is closely related to shell formation in some bivalves and gastropods. Up to now, few researches have been reported for cuttlebone formation in the class Cephalopoda. In this study, the structure and secretion function of cuttlebone sac of the golden cuttlefish Sepia esculenta was analyzed using the histological and histochemical methods. The results showed that high and columnar cells located in sac epithelium, and fiat cells existed near the base membrane. A lot of fibroblasts were found in the lateral mantle collective tissue. Some mucus, mucopolysaccharide and alkaline phosphatase （ALP） were found in the sac. The ultrastructural characteristics of Quasi-connective-tissue-calcium cells （QCTCC） were observed using a transmission electron microscope （TEM）. The relationship between cuttlebone sac secretion function and shell formation was discussed.

关键词： Sepia esculenta cuttlebone sac histology histochemistry transmission electron microscopy

Sperm Nuclear Expansion and Meiotic Maturation in Normal and Gynogenetic Eggs of the Scallop,Chlamys farreri

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Journal of Ocean University of China 2008年第1期7卷 103-107页

作者： PAN Ying LI Qi YU Ruihai wang rucai College of Fisheries Ocean University of China Qingdao 266003 P. R. China College of Animal Science and Technology Guangxi University Nanning 530004 P. R. China

Sperm nuclear expansion, meiosis and the association of the male and female pronuclei leading to the four-cell stage in normal Chlamys farreri eggs were observed under a fluorescence microscope. The effects of ultraviolet (UV) irradiation on the fer- tilizing sperm were also examined. Both normal and UV-irradiated sperm nuclei enlarged at three distinct phases (phase A, meta- phase I; phase B, polar body formation; and phase C, female pronuclear development and expansion) that were temporally correlated with meiotic process of the maternal chromosomes. Sperm nuclei underwent a rapid, initial enlargement during phase A, but con- densed slightly during phase B, then re-enlarged during phase C. The effects of UV irradiation were not apparent during transforma- tion of the sperm nucleus into a male pronucleus, and there was not any apparent effect on meiotic maturation and development of the female pronucleus. However, the rate of expansion of the UV-irradiated sperm nuclei and the size of male pronuclei were reduced apparently. Unlike the female pronucleus, the male pronucleus derived from sperm genome inactivated by UV irradiation did not form chromosomes, but became a dense chromatin body (DCB). At mitotic anaphase, DCB did not participate in the karyokinesis of the first cleavage as evidenced by chromosomal nondisjunction, demonstrating the effectiveness of using UV irradiation to induce gynogenetic scallop embryos.

关键词： Chlamys farreri sperm nuclear expansion ultraviolet irradiation gynogenesis

Seasonal Variation of the Glycogen Enzyme Activity in Diploid and Triploid Pacific Oyster Gonad During Sexual Maturation

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Journal of Ocean University of China 2007年第4期6卷 383-386页

作者： KONG Lingfeng wang Zhaoping YU Ruihai LI Qi wang rucai College of Fisheries Ocean University of China Qingdao 266003 P. R. China

The glycogen content and the activities of two key enzymes in glycogen metabolism, glycogen phosphorylase and gly- cogen synthetase, in the gonad of diploid and triploid Pacific oysters (Crassostrea gigas) were compared during maturation. The glycogen content in the gonad of diploids decreased with gametogenesis (by 85.7%), but the glycogen content in the gonad of trip- loids did not vary significantly. Activity of glycogen phosphorylase (GP) in the gonad of diploids decreased with gametogenesis (by 55.5%), while GP activity of triploids did not vary significantly during maturation. Activity of glycogen synthetase (GS) in the gonad of diploids increased slightly with gametogenesis, reaching a peak in June. Activity of GS declined sharply from June to July, which might be due to gonad spawning. GS activity of triploid oysters in spawning time (July and August) was significantly higher than that in other months, which might be explained with a ‘compensating’ mechanism for the higher glycogen content in triploids.

关键词： Pacific oyster diploid triploid glycogen metabolism gametogenesis

Current status of poly culture and rotation culture in Chinese mariculture industry

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Acta Oceanologica Sinica 2004年第3期23卷 505-512页

作者： wangrucai YANGJianmin ZHENGXiaodong FisheryCollege OceanUniversityofChinaQingdao266003China MarineFisheriesResearchInstituteofShandongProvince Yantai264000China

The status of polyculture and rotational culture in Chinese marine life is reviewed. Some successful experience is summarized.

关键词： polyculture rotation culture manculture

Polyploid Breeding and Sex Control in Maricultured Animals

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Bulletin of the Chinese Academy of Sciences 2001年第2期15卷 95-96页

作者： XiangJianhai, wang rucai, wang Zichen, Jiang Weiguo, Zhang Peijun, wang Qingyin, Guo Ximing, Li Fuhua, wang Zhaoping, Zhang Guofan, He Maoxian, Xu Cheng, Yang Aiguo, Yang Huiping and wang Suoan.

Polyploid breeding and sex con-trolling are conducive to lifting mari-culture *** of Insti-tute of Oceanology,CAS and sev-eral other institutes in China Haverecently developed relevanttechniques. This program,starting from1996,focuses on triplold inductionand breeding,tetraplold inductionmechanisms and sex controlling. At the beginning,there were still

关键词： Polyploid Breeding and Sex Control in Maricultured Animals

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几种帘形目贝类rDNA ITS序列的比较

高技术通讯 2007年第4期17卷 435-440页

作者：刘相全包振民胡景杰王师方建光王如才中国海洋大学生命科学与技术学部青岛266003 山东省海洋水产研究所烟台264006 中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用开放实验室青岛266071

扩增并测序了4种帘形目和1种蚶目贝类（外群）核糖体DNA的转录间隔区（ITS）序列。帘形目贝类序列长度在874bp到1466bp之间,GC含量在62%到67%之间,ITS序列在相近的3种帘蛤科贝类之间表现出长度保守性和碱基组成的相似性。采用ITS1,ITS2... 详细信息

扩增并测序了4种帘形目和1种蚶目贝类（外群）核糖体DNA的转录间隔区（ITS）序列。帘形目贝类序列长度在874bp到1466bp之间,GC含量在62%到67%之间,ITS序列在相近的3种帘蛤科贝类之间表现出长度保守性和碱基组成的相似性。采用ITS1,ITS2,以及ITS1与ITS2的连接序列进行聚类,结果表明：2种蛤仔聚为一枝,表现出较近的亲缘关系,帘蛤科的3种贝类聚在一起,再与同属帘形目的缢蛏相聚。研究的结果揭示了帘形目贝类的系统发生关系。

关键词：帘形目核糖体DNA 转录间隔区系统发生

Phylogeny of the cuttlefishes （Mollusca: Cephalopoda） based on COI and partial 16S rRNA gene sequences

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Phylogeny of the cuttlefishes （Mollusca: Cephalopoda） base...

贝类学会第七次会员代表大会暨第十一次学术讨论会

作者： XiaoDong Zheng rucai wang Qi Li Zhaoping wang The key Lab of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China

＜正＞ To clarify cuttlefish phylogeny, cytochrome c oxidase subunit I （COI） gene and partial 16S rRNA gene were sequenced for members of 7 Sepioid sepecies and several outgroups. Several phylogenetic trees were built by the neighbor-joining method. The coleoids were obviously be divided into two main lineages, Octopoda and Decapoda （Sepioiden + Teuthoidea）. The order Sepioidea, including the

关键词： Phylogeny Cephalopods Cuttlefish Sepiolidae 16S rRNA gene COI gene

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杨桦林经营密度的研究

东北林业大学学报 1999年第3期27卷 7-9页

作者：栾士波肖玉杰王汝才黑龙江省林业科学研究所哈尔滨150040 黑龙江省带岭林业实验局黑龙江省绥棱林业局

采用固定标准地定位观测和临时标准地调查点面结合的方法,研究了杨桦林的密度,得出了杨桦林的最大密度、最佳密度和最小密度数学模型,确定了杨桦林的最佳经营密度与最低经营密度分别为0．70和0．55。

关键词：杨桦林经营密度数学模型