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Bioaccumulation and transformation of cadmium by Phaeodactylum tricornutum

Science China Chemistry 2009年第12期52卷 2373-2380页

作者： SI DongFang YANG LiMin YAN Hua wang qiuquan Department of Chemistry&Key Laboratory of Analytical Sciences College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China State Key Laboratory of Marine Environmental Science Xiamen UniversityXiamen 361005China

In this paper,we report the bioaccumulation and transformation of cadmium (Cd) by Phaeodactylum tricornutum in the presence of ethylenediamine tetra acetic acid (EDTA) and cysteine (Cys). Both EDTA and Cys can alleviate the toxicity of Cd to P. tricornutum. Short term intracellular uptake and extracellular adsorption experiments using ICP-MS indicated that the amounts of Cd accumulated on the cell surface of P. tricornutum and inside the cell decreased along with the increase of EDTA concentration,which conformed to the prediction of the Free Ion Activity Model (FIAM). However,extracellular adsorption of Cd increased at first and then decreased along with the increase in the concentration of Cys,while intracellular uptake increased under Cys concentrations from the blank value to 4.45 μmol/L,and then tended to remain at the same level when the Cys concentration was greater than 4.45 μmol/L,and this deviated remarkably from the FIAM. The interactions of Cd with _Si_OH,_C_OH and NH2(CO) _OH on the cell wall were confirmed using FT-IR and XPS studies. The results obtained using HPLC of the phytochelatins (PCs) produced by P. tricornutum under CdCl2,Cd_EDTA and Cd_Cys stress suggested that the main reason for the different effects of EDTA and Cys on the bioaccumulation and transformation of Cd by P. tricornutum was that Cys is not only a complexing ligand to Cd,as is EDTA,but also it is a precursor of the intracellular synthesizing PCs participating in the cellular defense mechanism against Cd. Furthermore,the discovery of in vivo PCs and oxidized_PCs as well as Cd-PC2 in P. tricornutum using ESI-IT-MS provided the evidence for deactivation of Cd by the PCs,reducing Cd-toxicity to P. tricornutum.

关键词： Phaeodactylum tricornutum cadmium bioaccumulation and transformation cysteine EDTA

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Distribution of lanthanum among the chloroplast subcomponents of spinach and its biological effects on photosynthesis:location of the lanthanum binding sites in photosystem II

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Chinese Science Bulletin 2005年第16期50卷 1714-1720页

作者： YAN Wenwu YANG Limin wang qiuquan Department of Chemistry & the MOE Key Laboratory of Modem Analytical Sciences College of Chemistry and Chemical Engineering Xiamen University Xiamen 361006 China

The effects of lanthanum at different concen-trations on the related photosynthetic activities of Hill reac-tion, Mg2+-ATPase and Ca2+-ATPase in spinach chloroplast were studied. Experimental results showed that lanthanum can increase all the activities at suitable concentration (15-30 mg·L-1), however, it behaves toxically on them when over used (60 mg·L-1). To get an improved understanding of the mechanism of lanthanum effects on the photosynthesis of spinach, the different subcomponents in the chloroplast of the cultured spinach were isolated, and the content of lan-thanum in each subcomponent was determined by ICP-MS. The results obtained indicated that among these different subcomponents, about 90% out of the total chloroplast lan-thanum was located in photosystem II (PS II) while there was little lanthanum in photosystem I (PS I). Moreover, size ex-clusion high performance liquid chromatography (SE-HPLC) coupled with online UV and ICP-MS detections was novelly used for locating lanthanum binding sites in PS II proteins for the first time. It was found that lanthanum has two bind-ing sites in PS II: La associates with chlorophyll together with magnesium in PS II by partly replacing magnesium and also shares the common binding sites of PS II proteins to-gether with the inorganic cofactors of calcium and manga-nese, influencing the process of photosynthesis.

关键词：菠菜光合作用生物特性镧叶绿体

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Synergistic defensive mechanism of phytochelatins and antioxidative enzymes in Brassica chinensis L. against Cd stress

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Chinese Science Bulletin 2008年第10期53卷 1503-1511页

作者： CHEN LiQin GUO YiFei YANG LiMin wang qiuquan Department of Chemistry and the MOE Key Laboratory of Modern Analytical Science College of Chemistry and Chemical Engi- neering Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

Brassica chinensis L. was chosen and exposed to different concentrations of Cd exposure to evaluate its Cd-accumulating capacity and its potential cellular defensive mechanisms. Cd accumulation in the shoots and roots of B. chinensis was up to 1348.3±461.8 and 3761.0±795.0 mg per killogram of dry weight, respectively, under 200 μmol/L of Cd exposure. Increasing Cd accumulation in the plant was accompanied by rapid accumulation of phytochelatins (PCs), and the sequestration of Cd by PCs provided a primary cellular mechanism for Cd detoxification and tolerance of B. chinensis. Furthermore, malondialdehyde formation, hydrogen peroxide content and antioxidative enzyme activities such as superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase were observed in the shoots of Cd-stressed B. chinensis. Increasing enzyme activities in response to concentrations of 5 to 50 μmol/L Cd showed an efficient defense against oxidative stress, suggesting that the antioxidative system was a secondary defensive mechanism. These resulted in reduced free Cd damage and enhanced Cd accumulation and tolerance. Glutathione plays a pivotal role in these two detoxification pathways. In general, these results suggested that PCs and the antioxidative system are synergistic in combatting Cd-induced oxidative stress and that they play important roles in Cd detoxification of B. chinensis, and also give a deep understanding of the natural defensive mechanisms in plants under heavy metal stress.

关键词：镉芸苔植物化学酶类

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Studies of biouptake and transformation of mercury by a typical unicellular diatom Phaeodactylum tricornutum

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Chinese Science Bulletin 2013年第2期58卷 256-265页

作者： DENG GuiFu ZHANG TianWen YANG LiMin wang qiuquan Department of Chemistry & the Key Laboratory of Analytical Science College of Chemistry and Chemical EngineeringXiamen University State Key Laboratory of Marine Environmental Science Xiamen University

Mercury(Hg) is a toxic heavy metal with its biogeochemical cycling in the ocean depending on the type and behavior of the oceanic *** present work aimed to evaluate bioaccumulation and transformation of Hg by Phaeodactylum tricornutum,a typical unicellular diatom,when exposed to the extremely high level of Hg in order to understand the possible mechanisms of acute stress *** can accumulate Hg(its bioaccumulation factor is at 104 level),and the 96 h EC 50 was estimated to be 145μg *** amounts of surface-bound Hg being about 1.2 to 4.8 times higher than those of intracellular Hg under exposure to HgCl 2(from 20 to 120μg L-1 concentrations) suggested that the cell wall of *** is an important "fence" towards *** entering the *** cell,Hg underwent transformation in its chemical form via interactions with high molecular weight sulfur-containing proteins(accounting for 68% of the intracellular Hg),and glutathione as well as the induced phytochelatins(PCs)(24% Hg) which alleviated the toxicity of *** addition,the existence of organic ligands greatly influenced the uptake and transformation behavior of *** towards HgCl2,especially in the case of cysteine(Cys),which increased the uptake of Hg,but alleviated the toxicity of Hg towards *** due to the fact that Cys is an important precursor for the synthesis of PCs inside the *** uptake process of Hg by *** was in agreement with the Freundlich isotherm,suggesting a typical heterogeneous sorption *** importantly,we observed the conversion of HgCl2 into methylmercury inside the *** cells and its release into the culture solution using HPLC/CVG-AFS and GC-MS,although the mechanism needs to be further investigated.

关键词：三角褐指藻氯化汞单细胞改造硅藻生物地球化学循环 HgCl2 吸收过程

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dentification of AtOPT4 as a Plant Glutathione ;ransporter

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Molecular Plant 2016年第3期9卷 481-484页

作者： Zhongchun Zhang Qingqing Xie Timothy O. Jobe Andrew R. Kau Cun wang Yunxia Li Baosheng Qiu qiuquan wang David G. Mendoza-Cdzatl Julian I. Schroeder Division of Biological Sciences Cell and Developmental Biology Section University of California San Diego La Jolla CA 92093-0116 USA School of Life Sciences Central China Normal University Wuhan 430079 Hubei P.R. China Hubei Key Laboratory of Genetic Regulation and Integrative Biology Central China Normal University Wuhan 430079 Hubei P.R. China Department of Chemistry & the MOE Key Lab of Spectrochemical Analysis and Instrumentation College of Chemistry and Chemical Engineering Xiamen University Xiamen Fujian 361005 China Division of Plant Sciences C.S. Bond Life Sciences Center University of Missouri Columbia MO 65211 USA

Glutathione （GSH） is an essential tri-peptide important for several plant processes, including the control of cellular redox status, detoxification of xenobiotics, root development, heavy metal transport and resista... 详细信息

关键词： detoxification Cadmium phytochelatins

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HISTORICAL POLLUTION LEVEL OF ATMOSPHERIC H-POPS IN SOUTHEASTERN CHINA RECORDED IN TREE BARK AND PEATBOG

HISTORICAL POLLUTION LEVEL OF ATMOSPHERIC H-POPS IN SOUTHEAS...

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第十二届北京分析测试学术报告会暨展览会(BCEIA 2007)

作者： Yuli ZHAO Limin YANG qiuquan wang Department of Chemistry & the MOE Key Laboratory of Analytical Sciences College of Chemistry & Chemical Engineering Xiamen University

Historical pollution level of persistent organic pollutants(POPs)has been evaluated by the amount of POPs recorded in lake sediment,polar ice,coral,tree year-ring as well as herbarium ***,I will talk about the usefulness of tree bark pocket[1,2]and peatbog for evaluating the historical pollution levels of atmospheric halogenated POPs(H-POPs)in southeastern

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PHOTOCATALYTIC VAPOR GENERATION WITH NANO TiO2-AND ZrO2-BASED SEMICONDUCTOR FOR ELEMENTAL SPECIATION ANALYSIS USING HPLC-AFS

PHOTOCATALYTIC VAPOR GENERATION WITH NANO TiO2-AND ZrO2-BASE...

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第十四届北京分析测试学术报告会暨展览会(BCEIA 2011)

作者： Huamin LI Liming YANG qiuquan wang Department of Chemistry & the Key Laboratory of Analytical Sciences College of Chemistry and Chemical EngineeringXiamen University

Compared with the conventional pneumatic nebulization technique,chemical vapor generation(CVG)greatly improves the sample introduction efficiencyand thus the sensitivity of detection of atomic ***,photo-induced vapor ...

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A METHOD FOR THE ANALYSIS OF POLYBROMINATED DIPHENYL ETHERS IN ENVIRONMENTAL SAMPLES BY LARGE-VOLUME INJECTION GC/NEGATIVE CHEMICAL IONIZATION LOW-RESOLUTION MS

A METHOD FOR THE ANALYSIS OF POLYBROMINATED DIPHENYL ETHERS ...

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第十一届北京分析测试学术报告会暨展览会(BCEIA 2005)

作者： Yuli ZHAO Limin YANG qiuquan wang Department of Chemistry & the MOE Key Laboratory of Modern Analytical Sciences College of Chemistry and Chemical EngineeringXiamen University

A new method of programmable temperature vaporizer large-volume injection GC/Negative Chemical Ionization Low-Resolution MS was developed for the selective and sensitive determination of polybrominated diphenyl ethers...

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SEC-ICPMS AND ESI-MS/MS FOR EVALUATION OF THE BIOCHEMICAL RESPOND OF PLANTS TO HEAVY METAL STRESS

SEC-ICPMS AND ESI-MS/MS FOR EVALUATION OF THE BIOCHEMICAL RE...

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第十二届北京分析测试学术报告会暨展览会(BCEIA 2007)

作者： Liqin Chen Yifei Guo Limin Yang qiuquan wang Department of Chemistry & the MOE Key Laboratory of Analytical Sciences College of Chemistry and Chemical Engineering Xiamen University

Size-exclusion chromatography(SEC)coupled with ICP-MS and ESI-MS/MS have been demonstrated as powerful and promising techniques for screening and identifying the in-vivo metallopeptides,with accurate isotopic distribution assignment,in metal toxicological *** this presentation,I will talk about the identification of in-vivo Cd-PC and Hg-PC complexes in Brassica chinensis

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生物体系中硒代氨基酸的手性形态分析进展

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分析化学 2005年第9期33卷 1330-1334页

作者：黄晓佳王秋泉黄本立厦门大学化学系教育部现代分析科学重点实验室厦门大学化学系教育部现代分析科学重点实验室厦门361005

综述了近年来利用高效液相色谱法(HPLC)、气相色谱法(GC)以及毛细管电泳(CE)等分析手段与其它高灵敏检测器联用对生物体系中硒代氨基酸进行手性形态分析研究情况,参考文献40篇。

关键词：硒代氨基酸手性形态分析生物样品评述

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