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The progress of olfactory transduction and biomimetic olfactory-based biosensors

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Chinese Science Bulletin 2007年第14期52卷 1886-1896页

作者： WU ChunSheng wang lijiang ZHOU Jun ZHAO LuHang wang Ping Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China Department of Biochemistry and Molecular Biology Medical College of Zhejiang University Hangzhou 310058 China

Olfaction is a very important sensation for all animals. Recently great progress has been made in the research of olfactory transduction. Especially the novel finding of the gene superfamily encoding olfactory receptors has led to rapid advances in olfactory transduction. These advances also promoted the research of biomimetic olfactory-based biosensors and some obvious achievements have been obtained due to their potential commercial prospects and promising industrial applications. This paper briefly introduces the biological basis of olfaction, summarizes the progress of olfactory signal transduction in the olfactory neuron, the olfactory bulb and the olfactory cortex, outlines the latest devel- opments and applications of biomimetic olfactory-based biosensors. Finally, the olfactory biosensor based on light addressable potentiometric sensor (LAPS) is addressed in detail based on our recent work and the research trends of olfactory biosensors in future are discussed.

关键词：嗅觉传导仿生学生物传感器生物学

Neurochip Based on Light-addressable Potentiometric Sensor

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稀有金属材料与工程 2006年第A3期35卷 413-416页

作者： Qingjun Liu Hua Cai Ying Xu Lifeng Qin lijiang wang Ping wang Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Education MinistryDepartment of Biomedical Engineering Zhejiang University Hangzhou 310027 China State Key Laboratory of Transducer Technology Chinese Academy of Sciences Shanghai 200050 China

A novel neurochip based on light addressable potentiometric sensor (LAPS) is *** its light addressable *** problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled *** on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by *** results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.

关键词： neurochip cell-based biosensor hippocampal neurons light-addressable potentiometric sensor

A Novel Structure of LAPS Array for Cell-Based Biosensor

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稀有金属材料与工程 2006年第A3期35卷 51-54页

作者： Ying Xu Hua Cai Qingjun Liu Lifeng Qin lijiang wang Ping wang Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Education Ministry Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China State Key Lab of Transducer Technology Chinese Academy of Sciences Shanghai 200050 China

The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) *** depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of *** characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor *** LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also *** paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise *** characteristics of active recording array areas and cell culture conditions are measured.

关键词： light-addressable potentiometric sensor array LAPS sensitivity cell-based biosensor

Porous CuO nanowires as the anode of rechargeable Na-ion batteries

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Nano Research 2014年第2期7卷 199-208页

作者： lijiang wang Kai Zhang Zhe Hu Wenchao Duan Fangyi Cheng Jun Chen Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Chemistry College Collaborative Innovation Center of Chemical Science and Engineering Nankai University Tianjin 300071 China

We report the preparation of porous CuO nanowires that are composed of nanoparticles （-50 nm） via a simple decomposition of a Cu（OH）2 precursor and their application as the anode materials of rechargeable Na-ion batteries. The as-prepared porous CuO nanowires exhibit a Brunauer-Emmett-Teller （BET） surface area of 13.05 m^2.g^-1, which is six times larger than that of bulk CuO （2.16 m^2.g^-1）. The anode of porous CuO nanowires showed discharge capacities of 640 mA.h.g^-1 in the first cycle and 303 mA.h.g^-1 after 50 cycles at 50 mA.g^-1 The high capacity is attributed to porous nanostructure which facilitates fast Na-intercalation kinetics. The mechanism of electrochemical Na-storage based on conversion reactions has been studied through cyclic voltammetry, X-ray diffraction （XRD）, Raman spectroscopy, and high resolution transmission electron microscopy （HRTEM）. It is demonstrated that in the discharge process, Na＋ions first insert into CuO to form a CuⅡ1-x CuⅠ x O1-x/2solid and a Na2O matrix then CuⅡ1-xCu Ⅰ xO1-x/2 reacts with Na＋ to produce Cu2O, and finally Cu2O decompose into Cu nanoparticles enclosed in a Na2O matrix. During the charge process, Cu nanopartides are first oxidized to generate Cu2O and then converted back to CuO. This result contributes to the design and mechanistic analysis of high-performance anodes for rechargeable Na-ion batteries.

关键词： porous CuO nanowires anode materialelectrochemicalconversion reactions Na-ion batteries

The Escherichia coli O157:H7 DNA detection on a gold nanoparticle-enhanced piezoelectric biosensor

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Chinese Science Bulletin 2008年第8期53卷 1175-1184页

作者： wang lijiang WEI QingShan WU ChunSheng HU ZhaoYing JI Jian wang Ping Biosensor National Special Laboratory and Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education Zhejiang University Hangzhou 310027 China

This paper presents development of a quartz crystal microbalance (QCM) biosensor for real-time de- tection of E. coli O157:H7 DNA based on nanogold particles amplification. Many inner Au nanoparticles were immobilized onto the thioled surface of the Au electrode, then more specific thiolated sin- gle-stranded DNA (ssDNA) probes could be fixed through Au-SH bonding. The hybridization was in- duced by exposing the ssDNA probe to the complementary target DNA of E. coli O157:H7 gene eaeA, then resulted in a mass change and corresponding frequency shifts ( △f ) of the QCM. The outer avidin-coated Au nanoparticles could combine with the target DNA to increase the mass. The electro- chemical techniques, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were adopted to manifest and character each step. The target DNA corresponding to 2.0×103 colony forming unit (CFU)/mL E. coli O157:H7 cells can be detected by this biosensor, so it is practical to develop a sensitive and effective QCM biosensor for pathogenic bacteria detection based on specific DNA analy- sis. The piezoelectric biosensing system has potential for further applications, such as food safety and environment monitoring, and this approach lays the groundwork for incorporating the method into an integrated system for in-field bacteria detection.

关键词：压电生物受感器黄金纳米颗粒 DNA 大肠埃希氏菌

Resistance to rice blast (Pyricularia oryzae) caused by the expression of trichosanthin gene in transgenic rice plants transferred through agrobacterium method

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Chinese Science Bulletin 2000年第19期45卷 1774-1778页

作者： Xiaotian Ming lijiang wang Chengcai An Huayi Yuan Zhangliang Chen Peking Univ Coll Life Sci Natl Lab Prot Engn & Plant Genet Engn Beijing 100871 Peoples R China

The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and the expression of foreign gene have been proved in regenerated plants. In antifungal assay the degrees of rice blast (Pyricularia oryzae) infection of the transgenic plants expressing trichosanthin and expressing GUS gene as control have been evaluated. The differences such as the time of disease symptom observed, the number of infected plants and damaged leaves, the growth of infected plants of the two transgenic plants after being inoculated by rice blast (Pyricularia oryzae) are significant. The transgenic plants with trichosanthin gene grew faster than the plants with GUS gene, even when humidity environment was removed. The results show that the transgenic plants that expressed trichosanthin are able to delay the infection of rice blast compared with the plants as control. In addition, no damage caused by the expression of trichosanthin gene in transgenic plants has been

关键词： trichosanthin antifungi agrobacterium rice.

The development of taste transduction and taste chip technology

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Chinese Science Bulletin 2005年第14期50卷 1415-1423页

作者： LI Yan LIU Qingjun XU Ying CAI Hua QIN Lifeng wang lijiang wang Ping Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering ZheJiang University Hangzhou 310027 China

The intrinsic perception process of taste is ob- viously far less known than those of vision, audition, touch and olfaction. Despite that taste cells utilize a variety of sen- sory mechanisms to translate plenty of gustatory sensations such as sour, sweet, bitter, salty and umami into cellular sig- nals, gustatory perception mechanisms are still under explo- ration due to the lack of effective methods on cellular and molecular level. Recently the development of molecular bio- logical and electrophysiological studies has promoted explo- ration of olfactory and gustatory transduction and coding mechanisms dramatically. Based on the studies of artificial olfaction, artificial taste and cell-based biosensor in our laboratory, this paper reviews the current research on taste transduction mechanism. We introduce the recent advances in cell chip that combined biology with microelectronics, discuss taste cell chip as well as its potential of prospective application in taste transduction mechanism in detail and propose the research trends of taste chip in future.

关键词：味觉感觉器官细胞芯片分子生物学

A quantum-chemical study on the discharge reaction mechanism of lithium-sulfur batteries

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Journal of Energy Chemistry 2013年第1期22卷 72-77页

作者： lijiang wang Tianran Zhang Siqi Yang Fangyi Cheng Jing Liang Jun Chen Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) College of Chemistry Nankai University

Lithium-sulfur batteries have attracted a great interest in electrochemical energy conversion and storage, but their discharge mechanism remains not well understood up to now. Here, we report density functional theory （DFr） calculation study of the discharge mechanism for lithium-sulfur batteries which are based on the structure of $8 and Li2Sx （l_〈x〈_8） clusters. The results show that for LizSz （1 〈x_8） clusters, the most stable geometry is chainlike when x = 1 and 6, while the minimal-energy structure is found to be cyclic when x = 2-5, 7, 8. The stability of LizSx （l_〈x_〈 8） clusters increases with the decreasing x value, indicating a favorable thermodynamic tendency of transition from $8 to Li2S. A three-step reaction route has been proposed during the discharge process, that is, $8---~Li2S4 at about 2.30 V, Li2S4---~Li2S2 at around 2.22 V, and Li2S2 ~ Li2S at 2.18 V. Furthermore, the effect of the electrolyte on the potential platform has been also investigated. The discharge potential is found to increase with the decrease of dielectric constant of the electrolyte. The computational results could provide insights into further understanding the discharge mechanism of lithium-sulfur batteries.

关键词： lithium-sulfur battery density functional theory discharge mechanism lithium polysulfide discharge potential

DISCUSSING　ON　SOLID　ABRASIVE　LAPPING　PATH

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Chinese Journal of Mechanical Engineering 1997年第2期10卷 101-105,140页

作者： Yang Jiandong ,Wen XuehengChangchun Institute of Optics and Fine MechanicsZhu Yanqiu, wang lijiang Jilin University of Technology

Relative motion path between lapping tool and workpiece in plane solid abrasive lapping is dis- cussed and its path model is set up. The paths simulate. by this model are compared with the one got in experiments. The changing relationship of workpiece rotating speed with the main spindle rotating speed and eccentricity is also discussed.

关键词： Lapping ,Path Solid abrasive