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Desiccation and osmotic resistance mechanism of Cronobacter spp. isolated from powdered infant formula

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Food Science and Human Wellness 2024年

作者： Shiqian Fu Danliangmin Song Xue Qin lihan wang Qianyu Zhao Chaoxin Man Xinyan Yang Yujun Jiang Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province， Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition， College of Food and Pharmaceutical Sciences， Ningbo University Key Laboratory of Dairy Science， Ministry of Education， Department of Food Science， Northeast Agricultural University

Cronobacter spp. has strong resistance to desiccation and high permeability in Enterobacteriaceae， and powdered infant formula (PIF) is one of the main contamination routes. In recent years， the contamination of Cronobacter spp. in PIF incidents occur from time to time， causing infant serious diseases or death. In this investigation， matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment. Subsequently， the isolates were evaluated for drying and osmotic pressure tolerance. The results showed that the deactivation rate of the strains ranged from 9.01% to 77.57%， and the highest osmotic pressure condition the strains could tolerate was 6% NaCl. In addition， there was a positive correlation between biofilm formation ability and desiccation resistance. Combined with transcriptomics， Cronobacter spp. can activate biofilm synthesis， produce more trehalose， accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions. 31 and 43 genes were found related to desiccation and permeability resistance， respectively. And some genes (cysM， thuF， ycjO， et al) were found to be associated with two tolerances for the first time.

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蛋白质修饰组学-中药作用机制研究的新途径

中国实验方剂学杂志 2024年第17期30卷 211-219页

作者：王砺寒侯静怡张晶晶杨洪军中国中医科学院医学实验中心中医药防治重大疾病基础研究北京市重点实验室北京100700 中国中医科学院中药研究所北京100700

中医药作为我国宝贵的科学遗产与文化瑰宝,承载着中华民族数千年智慧的结晶,其深厚的医学理论和丰富的实践经验在我国卫生保健事业中占据着不可替代的核心地位。面对现代医学挑战与健康需求,深入探究中药作用机制显得尤为迫切和必要。... 详细信息

中医药作为我国宝贵的科学遗产与文化瑰宝,承载着中华民族数千年智慧的结晶,其深厚的医学理论和丰富的实践经验在我国卫生保健事业中占据着不可替代的核心地位。面对现代医学挑战与健康需求,深入探究中药作用机制显得尤为迫切和必要。蛋白质翻译后修饰(PTM),作为调控蛋白质功能复杂性和多样性的核心机制,是连接内外环境刺激与机体内在生理反应的关键桥梁。同时,不同类型PTM之间可相互作用形成复杂的调控网络,符合中药多成分、多靶点的作用特点。尽管蛋白质修饰组学作为探究PTM的有效工具,近些年已经被应用于一些中药作用机制研究,但总体而言,这一领域的研究在中药学领域尚处于初步发展阶段。为此,该文首先概述了PTM的基础理论和蛋白质修饰组学的基本研究流程,继而系统回顾了蛋白质修饰组学在中药作用机制研究领域的应用现状,并针对现存研究局限性进行了深入剖析。该文旨在探寻一种基于PTM视角解析中药作用机制的创新研究范式,为未来深化理解中药作用机理提供理论依据与实践指导。

关键词：蛋白质翻译后修饰蛋白质修饰组学中药研究策略复杂作用机制解析

铁死亡在代谢相关性脂肪性肝病中的作用及中药干预进展

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中医药学报 2024年第4期52卷 105-111页

作者：王丽寒王锐牛捷杨婧黑龙江中医药大学基础医学院黑龙江哈尔滨150040 黑龙江中医药大学药学院黑龙江哈尔滨150040

代谢相关脂肪性肝病(MAFLD)是常见的慢性肝病之一,随着生活方式的改变该病也趋于年轻化。MAFLD的发病机制复杂,目前尚无治疗该病的特性药物。中医药在治疗慢性肝病上有悠久的历史和独特的优势。中药可通过调控铁死亡的信号通路达到抗炎... 详细信息

代谢相关脂肪性肝病(MAFLD)是常见的慢性肝病之一,随着生活方式的改变该病也趋于年轻化。MAFLD的发病机制复杂,目前尚无治疗该病的特性药物。中医药在治疗慢性肝病上有悠久的历史和独特的优势。中药可通过调控铁死亡的信号通路达到抗炎、抗纤维化、改善肝损伤的作用。因此本文简要阐述铁死亡在MAFLD中的机制,研究中药调节铁死亡在MAFLD中的作用,为进一步研究中医药治疗MAFLD提供参考。

关键词：铁死亡代谢相关脂肪性肝病中医药机制

OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak

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Journal of Integrative Plant Biology 2022年第5期64卷 1116-1130页

作者： lihan wang Jie Chen Yuqin Zhao Shiping wang Meng Yuan National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China

Rice OsLIC encoding a CCCH zinc finger transcription factor plays an important role in ***,the immune signaling pathways that OsLIC-involved and the underlying mechanisms that OsLIC-conferred resistance against pathogens are largely ***,we show that OsLIC,as a substrate for Os MAPK6,negatively regulates resistance to Xanthomonas oryzae ***(Xoo)and *** ***(Xoc)by directly suppressing OsWRKY30 *** assays showed that OsLIC bound to OsWRKY30 promoter and suppressed its *** assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc,knocking out OsWRKY30 in the oslic mutants attenuated the resistance against bacterial *** MAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity,therefore,overexpression of OsLIC partially suppressed Os MAPK6-mediated rice *** addition,both Os MAPK6-phosphorylated activation of OsLIC and phosphorylation-mimic OsLIC_(5D) had reduced DNA-binding activity towards OsWRKY30 promoter,thereby promoting OsWRKY30 ***,these results reveal that Os MAPK6-mediated phosphorylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription,suggesting that Os MAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens.

关键词： OsLIC OsMAPK6 OsWRKY30 rice Xanthomonas oryzae

Impaired SWEET-mediated sugar transportation impacts starch metabolism in developing rice seeds

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The Crop Journal 2022年第1期10卷 98-108页

作者： Pei Li lihan wang Hongbo Liu Meng Yuan National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070HubeiChina

Sugar transportation and sugar-to-starch metabolism are considered important processes in seed development and embryo viability.A few plant SWEET proteins acting as sugar transporters have been reported to function in inflorescence and/or seed ***,we identified seven members of the 21 Os SWEET genes in rice that play essential roles in sugar transportation and sugar-to-starch conversion in seed *** Os SWEET genes exhibiting different expression patterns during inflorescence and seed development were knocked out individually by CRISPR/*** third of the mutants showed decreased fertile pollen viability and shriveled mature caryopses,resulting in weakened seed *** fill-related genes but not representative grain shape-regulating genes showed attenuated expression in the *** of each of these mutants accumulated more sucrose,glucose or fructose but less *** all Os SWEET genes,Os SWEET4 and Os SWEET11 had major effects on caryopsis *** sugar-to-starch metabolic pathway was significantly altered in ossweet11 mutants based on differential expression analysis in RNA sequencing assays,confirming that Os SWEET11 functions as a sugar transporter with a key role in seed *** results help to decipher the multiple functions of Os SWEET genes and to show how they might be used in genetic improvement of rice.

关键词： Oryza sativa Sugar transporter SWEET Sugar Starch

Thermal stability of polyphenylsilsesquioxane-modified meta-aramid insulation paper

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High Voltage 2020年第3期5卷 264-269页

作者： Chao Tang Wei Zheng lihan wang Jufang Xie College of Engineering and Technology Southwest University400715 ChongqingPeople's Republic of China

The development of new materials for use in advanced distribution transformers is required to improve their heat resistance rating,operational safety,and *** combination of polymer and nanoparticles is the preferred approach to improve the thermal stability of *** this study,the thermal stability of laboratory-prepared meta-aramid insulation paper modified with polyphenylsilsesquioxane(PPSQ)was *** results showed that the thermal stability of the modified insulation paper was superior to that of the unmodified *** dynamics simulations were used to analyse the internal mechanism of the performance improvement achieved by modification with *** a microscopic perspective,the addition of PPSQ provided a relatively larger structural space for the meta-aramid fibre and increased the free volume of the entire system,which tightened the meta-aramid fibre molecular chains,increased the density of the structure of the paper,and enhanced the mutual permeability between meta-aramid fibre ***,the stress can be better transmitted,the performance loss such as phase delamination was effectively prevented,and the bonding between the meta-aramid fibres was strengthened.

关键词： stability insulation fibre

Novel apical-to-femoral rail technique for horizontal aorta in transcatheter aortic valve replacement

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第7期23卷 613-616页

作者： Xianbao LIU Hanyi DAI lihan wang Jiaqi FAN Jian’an wang Department of Cardiology the Second Affiliated HospitalZhejiang University School of MedicineHangzhou310009China Internal Medicine Zhejiang University School of MedicineHangzhou310058China

Transcatheter aortic valve replacement(TAVR)has emerged as a viable treatment option for patients with severe aortic stenosis regardless of its surgical risk stratification(Otto et al.,2021).Aortic angulation is usually measured as the angle between the horizon-tai and the aortic annulus planes based on preprocedural multidetector computed tomography(MDCT)(Al-Lamee et al.,2011).Extremely horizontal aorta,defined as an aortic angulation greater than 70°.

关键词： aortic viable

Exploring Brain Age Calculation Models Available for Alzheimer's Disease

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Journal of Beijing Institute of Technology 2023年第2期32卷 181-187页

作者： lihan wang Honghong Liu Weijia Liu Qunxi Dong Bin Hu School of Computer Science Beijing Institute of TechnologyBeijing 100081China School of Medical Technology Beijing Institute of TechnologyBeijing 100081China

The advantages of structural magnetic resonance imaging(sMRI)-based multidimensional tensor morphological features in brain disease research are the high sensitivity and resolution of sMRI to comprehensively capture the key structural information and quantify the structural ***,its direct application to regression analysis of high-dimensional small-sample data for brain age prediction may cause“dimensional catastrophe”.Therefore,this paper develops a brain age prediction method for high-dimensional small-sample data based on sMRI multidimensional morphological features and constructs brain age gap estimation(BrainAGE)biomarkers to quantify abnormal aging of key subcortical structures by extracting subcortical structural features for brain age prediction,which can then establish statistical analysis models to help diagnose Alzheimer’s disease and monitor health conditions,intervening at the preclinical stage.

关键词： brain age gap estimation(BrainAGE) Alzheimer’s disease(AD) structural magnetic resonance imaging(sMRI)

Increased leptin by hypoxic-preconditioning promotes autophagy of mesenchymal stem cells and protects them from apoptosis

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Science China(Life Sciences) 2014年第2期57卷 171-180页

作者： wang lihan HU XinYang ZHU Wei JIANG Zhi ZHOU Yu CHEN PanPan wang JianAn Cardiovascular Key Lab of Zhejiang Province the Second Affiliated Hospital School of Medicine Zhejiang University

Autophagy is the basic catabolic progress involved in cell degradation of unnecessary or dysfunctional cellular *** has been proven that autophagy could be utilized for cell survival under ***-preconditioning(HPC)could reduce apoptosis induced by ischemia and hypoxia/serum deprivation(H/SD)in bone marrow-derived mesenchymal stem cells(BMSCs).Previous studies have shown that both leptin signaling and autophagy activation were involved in the protection against apoptosis induced by various stress,including ***,it has never been fully understood how leptin was involved in the protective effects conferred by *** the present study,we demonstrated that HPC can induce autophagy in BMSCs by increased LC3-II/LC3-I ratio and autophagosome ***,similar effects were also observed when BMSCs were pretreated with *** beneficial effects offered by HPC were absent when BMSCs were incubated with autophagy inhibitor,3-methyladenine(3-MA).In addition,down-regulated leptin expression by leptin-shRNA also attenuated HPC-induced autophagy in BMSCs,which in turn was associated with increased apoptosis after exposed to sustained H/***,increased AMP-activated protein kinase phosphorylation and decreased mammalian target of rapamycin phosphorylation that were observed in HPC-treated BMSCs can also be attenuated by down-regulation of leptin *** data suggests that leptin has impact on HPC-induced autophagy in BMSCs which confers protection against apoptosis under H/SD,possibly through modulating both AMPK and mTOR pathway.

关键词： BMSCs autophagy hypoxic-preconditioning leptin apoptosis