检索结果-南通市图书馆

同方期刊数据库

Meta-Analysis:Prevalence of Food Allergy and Food Allergens—China, 2000−2021

在线全文

学校读者我要写书评

暂无评论

China CDC weekly 2022年第34期4卷 766-770,I0003-I0005页

作者： Jiangzuo Luo Qiuyu Zhang Yanjun Gu junjuan wang Guirong Liu Tao He Huilian Che Key Laboratory of Precision Nutrition and Food Quality Key Laboratory of Functional DairyMinistry of EducationCollege of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina

What is already known about this topic?In recent decades,the prevalence of food allergy has increased worldwide;however,a comprehensive estimate of the prevalence of food allergy and allergens in China is not yet *** is added by this report?By searching the English databases PubMed,Embase,Cochrane Library,and Chinese databases CNKI,Wanfang Data,and VIP Chinese epidemiological studies on food allergy,the probability of food allergy in China and related influencing factors were *** are the implications for public health practice?The findings of this study provide up-to-date estimates of the prevalence of food allergy rates in China in terms of age,gender,and the eight major food allergens.

关键词： has searching gender

Genome-wide identification and expression analysis of DNA demethylase family in cotton

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cotton Research 2019年第3期2卷 142-150页

作者： YANG Xiaomin LU Xuke CHEN Xiugui wang Delong wang junjuan wang Shuai GUO Lixue CHEN Chao wang Xiaoge wang Xinlei YE Wuwei Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology Key Laboratory for Cotton Genetic ImprovementMOAA

Background:DNA methylation is an important epigenetic factor that maintains and regulates gene *** mode and level of DNA methylation depend on the roles of DNA methyltransferase and demethylase,while DNA demethylase plays a key role in the process of DNA *** results showed that the plant’s DNA demethylase all contained conserved DNA glycosidase *** study identified the cotton DNA demethylase gene family and analyzed it using bioinformatics methods to lay the foundation for further study of cotton demethylase gene ***:This study used genomic information from diploid Gossypium raimondii JGI(D),Gossypium arboreum ***(A),Gossypium hirsutum ***(AD1) and Gossypium barbadebse L NAU(AD2) to Arabidopsis *** DNA demethylase genes sequence of Arabidopsis as reference,25 DNA demethylase genes were identified in cotton by BLAST *** are 4 genes in the genome D,5 genes in the genome A,10 genes in the genome AD1,and 6 genes in the genome *** gene structure and evolution were analyzed by bioinformatics,and the expression patterns of DNA demethylase gene family in Gossypium hirsutum L were *** the phylogenetic tree analysis,the DNA demethylase gene family of cotton can be divided into four subfamilies:REPRESSOR of SILENCING 1(ROS1),DEMETER(DME),DEMETER-LIKE 2(DML2),and DEMETER-LIKE3(DML3).The sequence similarity of DNA demethylase genes in the same species was higher,and the genetic relationship was also relatively *** of the gene structure revealed that the DNA demethylase gene family members of the four subfamilies varied *** them,the number of introns of ROS1 and DME subfamily was larger,and the gene structure was more *** the analysis of the conserved domain,it was known that the DNA demethylase family gene member has an endonuclease Ⅲ(END03 c) ***:The genes of the DNA demethylase family are distributed differently in different cotton species,and the ge

关键词： DNA demethylase Gossypium arboreum L. Gossypium barbadebse L. Gossypium hirsutum L. Gossypium raimondii

Characters and structures of the nucleobase-ascorbate transporters(NAT)family genes in Gossypium hirsutum and their roles in responding to salt and drought stresses

在线全文

学校读者我要写书评

暂无评论

Journal of Cotton Research 2022年第2期5卷 115-129页

作者： GUO Lixue ZHAO Lanjie LU Xuke CHEN Xiugui wang Shuai wang junjuan wang Delong YIN Zujun CHEN Chao FAN Yapeng ZHANG Yuexin YE Wuwei State Key Laboratory of C otton Biology/I nstitute of Cott on Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base School of Agricultural SciencesZhengzhou University/Key Laboratory for Cotton Genetic ImprovementMOARAAnyang 455000HenanChina

Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in ***: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) ***: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to b

关键词： Gossypium hirsutum NAT gene family VIGS Salt and drought stresses

Functional structure analysis and genome‑wide identification of CNX gene family in cotton

在线全文

学校读者我要写书评

暂无评论

Journal of Cotton Research 2022年第3期5卷 285-301页

作者： XU Nan ZHANG Hong ZHANG Yuexin FAN Yapeng wang Jing MALIK Waqar Afzal RUI Cun HAN Mingge LU Xuke CHEN Xiugui wang junjuan wang Delong wang Shuai CHEN Chao GUO Lixue ZHAO Lanjie YE Wuwei Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Zhengzhou Research Base State Key Laboratory of Cotton Biology/School of Agricultural SciencesZhengzhou UniversityAnyang 455000HenanChina

Background:Under abiotic stress conditions,cotton growth is inhibited and yield losses are *** of calnexin family members and function analysis under abiotic stress laid the foundation for the screening of stressrelated candidate ***:A total of 60 CNX family members have been identified in Gossypium hirsutum,***,***,and ***,and they were divided into two categories:CNX and CRT *** the construction of a phylogenetic tree,they were subdivided into three *** analysis of chromosome localization,conserved promoters,gene structure and selection under pressure showed that the family members were highly conserved in the evolution *** of cis-acting elements in the promoter regions showed that CNX family genes contain regulatory elements for growth and development,anaerobic,drought,defense and stress response,and plant *** RNA-seq data to study the expression pattern of GhCNX genes under cold,hot,salt stress and Polyethylene glycol,it was observed that the gene expression levels changed by different degrees under different stress conditions,indicating that GhCNX members were involved in the regulation of multiple biological ***:This study provides an insight into the members of cotton CNX *** results of this study suggested that CNX family members play a role in defense against adversity and provide a foundation for the discovery of stress-related genes.

关键词： Calnexin GhCNX Conserved motifs Selection pressure Collinearity Differential expression

Comparative Analysis of Salinity-Induced Proteomic Changes in Cotton (Gossypium hirsutum L.)

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences 2015年第1期6卷 78-86页

作者： Yupeng Cui Xuke Lu Delong wang junjuan wang Zujun Yin Weili Fan Shuai wang Wuwei Ye State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural Sciences Anyang China

Salt stress on cotton varieties of distinct salinity tolerance can induce expression of different proteins. Zhong 07, a salt-tolerant variety and Zhong s9612, a salt-sensitive variety, were utilized as experimental materials. The leaves of trefoil seedlings treated with or without 0.4% NaCl for 24 h were harvested for whole-protein extraction. Two-dimensional technology, combined with mass spectroscopy (MS) analysis and protein database searching, was employed to detect differentially expressed proteins and determine their identities and biological functions. Compared with the control, Zhong 07 showed 10 differentially expressed proteins under salt stress, of which 6 were upregulated and 4 were downregulated. Meanwhile, 12 differentially expressed proteins were detected in Zhong s9612 under salt stress, of which 10 were upregulated and 2 were downregulated. In the matrix-assisted laser desorption-ionization/time of flight-time of flight/MS analysis, 14 differentially expressed proteins were successfully identified, including the ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisco) large subunit-binding protein subunit alpha (RuBisco α), luminal binding protein (LBP), heat shock protein 70 (Hsp1, 2, 3), pathogenesis-related protein class 10 (PR-10), quinoneoxidoreductase-like protein (QOR), S-adenosylmethioninesyn-thetase (SAMS), enolase (EN), and RuBisco large subunit-binding protein subunit beta (RuBisco β). Cellular function is ultimately executed by proteins, and cotton varieties with different salt tolerance can be influenced by salt stress to various degrees, which can provide certain theoretical foundation for the identification of salt tolerance of cotton varieties. The findings also provide some proteins, such as the RuBisco large subunit binding proteins α and β subunits, OEE2 protein, HSP70, and S-adenosylmethionine synthetase, which can be used as protein markers of salt-to-lerance before- and post-treatment, making a big difference in salt-toleranc

关键词： Upland Cotton (Gossypium hirsutum L.) Salt Stress Differential Protein Mass Spectrum Analysis

Identification and Characterization of Salt-responsive MicroRNAs and Their Target Genes in Cotton

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification and Characterization of Salt-responsive Micro...

Transcriptome-Wide Identification of Salt-Responsive miRNA Targets in Cotton Using Degradome Sequencing

2015年全国棉花青年学术研讨会

作者： Yin Zujun wang junjuan wang Delong Fan Weili wang Shuai Ye Wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Soil salinity is one of the most serious factors limiting the productivity of agricultural *** salt concentrations in the soil affect various aspects of plant growth and development,such as inhibition of enzymatic activities and reductions in photosynthetic *** respond to stress using multiple gene regulatory mechanisms including post-transcriptional regulation of gene ***（miRNAs）,an abundant class of short non-coding（21-23 nt） RNAs,are generated from single-strand hairpin RNA precursors and are sometimes conserved over long evolutionary *** regulate gene expression by binding to targeted mRNA sequences in a perfect or near-perfect complementary site,leading to mRNA cleavage or,in a few cases,translational repression in *** this study,three cotton small RNA libraries were constructed and sequenced under normal conditions,short-term salt stress（SS） and long-term salt stress（LS）.The length distribution of obtained small RNAs was significantly different between *** SS sample showed significantly higher 21-nt small RNA abundance（48.86%） than CK（40.63%） and the LS sample（36.31%）.To investigate the repertoire of miRNAs,small RNA reads in three small RNA libraries were first mapped to protein-coding and structural RNA-coding regions in the Rfam and GenBank ***,tRNAs,snRNAs and snoRNAs were ***,the remaining sequences were aligned with all known miRNAs in the central miRNA Registry Database,miRBase *** a result,a total of 201 conserved miRNAs were *** were classified into 88 families on the basis of sequence *** remaining non-encoding sequences were used to predict novel miRNAs using the MIREAP program,based on their secondary *** 24 of them,the complementary miRNA species were cloned in libraries,providing an indication of precise excision from the stem-loop *** first made a comparative analysis of miRNA expression between CK and SS lib

关键词： cotton microRNAs target genes ARFs

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Transcriptome-Wide Identification of Salt-Responsive miRNA T...

Identification and Expression Analysis Mitogen-Activated Protein Kinase Signalling Cascades in Cotton

中国农学会棉花分会2016年年会

作者： Yin Zujun wang junjuan wang Delong Fan Weili wang Shuai Guo Lixue Ye Wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

＜正＞MicroRNAs（miRNAs）are a class of endogenous,non-coding small RNAs that play important roles in many developmental processes and stress responses in *** finely modulate gene expression by binding to targeted mRN...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification and Expression Analysis Mitogen-Activated Pro...

Cloning and Ectopic Expression of <i>ScYCF</i>1 Gene from <i>Saccharomyces cerevisiae</i>in Cotton

中国农学会棉花分会40年征文暨2019年年会

作者： Zhu Weidong wang junjuan wang Delong wang Shuai Guo Lixue Yin Zujun Ye Wuwei State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences

Mitogen-activated protein kinase(MAPK) cascades are involved in plant growth and development,as well as play a central role in response to biotic and abiotic ***,few complete MAPK signaling cascades have been characterized in *** this study,a total of 169 MAPKKK,25 MAPKK and 56 MAPK genes were identified in Gossypium hirsutum genome and classified into distinct groups supported by the phylogenetic *** structures and conserved motifs analyses pointed out that the majority of MAPKKK,MAPKK and MAPK genes in the same subfamily shared similar gene structures and conserved ***,analyses of cis-acting regulatory elements in promoter regions revealed that MAPK cascades might participate in plant responses to diverse abiotic stresses and ***,the different stages of transcriptome analysis demonstrated that 39 MAPKKK genes,10 MAPKK genes and 20 MAPK genes play an important role in drought stress *** the similarity of expression patterns could indicate the correlation and the similar biological function of *** addition,interaction networks analyses of three gene families suggest that MAPK signalling cascade mediate network participates in multiple stress *** are some high confidence interactive proteins involved in MAPKKK,MAPKK and MAPK networks,including MEKK1,WRKY33,MKKs,and ***,our study will provide a useful reference for further functional analysis of MAPK cascades in cotton.

关键词：

来源： cnki会议 cnki会议评论

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences 2018年第1期9卷 53-68页

作者： Min Mu Na Shu Xuke Lu Xiugui Chen Shuai wang junjuan wang Delong wang Weili Fan Lixue Guo Chao Chen Wuwei Ye State Key Laboratory of Cotton Biology Chinese Academy of Agricultural Sciences Cotton Research Institute Anyang China

Yeast cadmium factor 1 (YCF1), is a member of the ATP-binding cassette (ABC) transporter family. To explore the functions of YCF1 of Saccharomyces cerevisiae (ScYCF1) in the cotton, ScYCF1 was cloned from Saccharomyces cerevisiae As2.375, with the full-length of 4548 bp. The bioinformatics analysis revealed that the largest component of ScYCF1 protein is leucine (12%). ScYCF1 is alkaline and positive charged, stable, and hydrophilic protein. The predictive secondary structure is mainly composed of α-helix areas, random coils and β-sheets. We constructed the pBI121-ScYCF1:GFP infusion expression vector and verified it by enzyme ingestion. The transient expression results of cotton pollen showed that the green fluorescence phenomenon of three kinds of upland cotton pollen significantly increased after transforming ScYCF1. The salt sensitive material upland cotton CCRI12 was transformed in vivo simultaneously, and the germination ability of trans-ScYCF1-gene T0 seeds was much better than the acceptor material CCRI12 under the stress of 100 mM NaCl saline solution. According to the gene nucleotide sequences, four pairs of primers were designed for molecular detection of T0 generation, and the sequencing results of PCR products of four specific primers evidence that the transgene is successful. Salt tolerance analysis of leaf discs of identified transgenic cotton showed that the chlorophyll content of leaf discs of transgenic cotton was higher than the content of the control cotton under salt stress. ScYCF1 gene was cloned and introduced into cotton, showing that ScYCF1 plays an important role in improving the salt tolerance of cotton.

关键词： Saccharomyces cerevisiae ScYCF1 Upland Cotton Pollen Instantaneous Expression Molecular Detection